针对短期电力负荷在线预测问题,结合多变量相空间重构以及多核函数最小二乘支持向量机(least squares support vector machine,LS-SVM),提出一种基于滑动窗口策略与改进人工鱼群算法(artificial fish swarm algorithm,AFSA)的短期电力...针对短期电力负荷在线预测问题,结合多变量相空间重构以及多核函数最小二乘支持向量机(least squares support vector machine,LS-SVM),提出一种基于滑动窗口策略与改进人工鱼群算法(artificial fish swarm algorithm,AFSA)的短期电力负荷在线预测综合优化方法。首先,利用多变量相空间重构还原真实电力系统动力学特性;然后,将核函数进行排列组合,从而将组合核函数的构造问题转换为权值系数的优化问题;进一步,将延迟时间、嵌入维数、LS-SVM参数及核函数权值作为整体参数向量,利用混沌自适应人工鱼群算法对训练数据预测精度的适应度函数进行优化,从而得到最优的预测模型参数;最后,通过滑动时窗策略将得到的预测模型对短期电力负荷进行在线预测,结果证明了提出方法的有效性。展开更多
Objective:To investigate the effect and possible mechanism of hydroxysafflor yellow A(HSYA) on human immortalized keratinocyte cell proliferation and migration.Methods:HaCaT cells were treated with HSYA.Cell prolifera...Objective:To investigate the effect and possible mechanism of hydroxysafflor yellow A(HSYA) on human immortalized keratinocyte cell proliferation and migration.Methods:HaCaT cells were treated with HSYA.Cell proliferation was detected by the cell counting kit-8 assay,and cell migration was measured using wound healing assay and Transwell migration assay.The mRNA and protein expression levels of heparin-binding epidermal growth factor(EGF)-like growth factor(HBEGF),EGF receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),mammalian target of rapamycin(mTOR),and hypoxia-inducible factor-1α(HIF-1α) were detected by quantitative real-time polymerase chain reaction(qRT-PCR) and Western blot,respectively.Circ_0084443-overexpressing HaCaT cells and empty plasmid HaCaT cells were constructed using the lentiviral stable transfection and treated with HSYA.The expression of circ_0084443 was detected by qRT-PCR.Results:HSYA(800 μmol/L) significantly promoted HaCaT cell proliferation and migration(P<0.05or P<0.01).It also increased the mRNA and protein expression levels of HBEGF,EGFR,PI3K,AKT,mTOR and HIF-1α,and increased the phosphorylation levels of PI3K and AKT(P<0.05 or P<0.01).Furthermore,HSYA promoted HaCaT cell proliferation and migration via the HBEGF/EGFR and PI3K/AKT/m TOR signaling pathways(P<0.01).Circ_0084443 attenuated the mRNA expression levels of HBEGF,EGFR,PI3K,AKT,mTOR and HIF-1α(P<0.05).HSYA inhibited the circ_0084443 expression,further antagonized the inhibition of circ_0084443on HBEGF,EGFR,PI3K,AKT,m TOR and HIF-1α,and promoted the proliferation of circ_0084443-overexpressing HaCaT cells(P<0.05 or P<0.01).However,HSYA could not influence the inhibitory effect of circ_0084443 on HaCaT cell migration(P>0.05).Conclusion:HSYA played an accelerative role in HaCaT cell proliferation and migration,which may be attributable to activating HBEGF/EGFR and PI3K/AKT signaling pathways,and had a particular inhibitory effect on the keratinocyte negative regulator circ_0084443.展开更多
文摘针对短期电力负荷在线预测问题,结合多变量相空间重构以及多核函数最小二乘支持向量机(least squares support vector machine,LS-SVM),提出一种基于滑动窗口策略与改进人工鱼群算法(artificial fish swarm algorithm,AFSA)的短期电力负荷在线预测综合优化方法。首先,利用多变量相空间重构还原真实电力系统动力学特性;然后,将核函数进行排列组合,从而将组合核函数的构造问题转换为权值系数的优化问题;进一步,将延迟时间、嵌入维数、LS-SVM参数及核函数权值作为整体参数向量,利用混沌自适应人工鱼群算法对训练数据预测精度的适应度函数进行优化,从而得到最优的预测模型参数;最后,通过滑动时窗策略将得到的预测模型对短期电力负荷进行在线预测,结果证明了提出方法的有效性。
基金Supported by the Natural Science Fund of Liaoning Provincial Science and Technology Department (No.20180530070)Science and Technology Innovation Foundation of Dalian (No.2020JJ27SN073)。
文摘Objective:To investigate the effect and possible mechanism of hydroxysafflor yellow A(HSYA) on human immortalized keratinocyte cell proliferation and migration.Methods:HaCaT cells were treated with HSYA.Cell proliferation was detected by the cell counting kit-8 assay,and cell migration was measured using wound healing assay and Transwell migration assay.The mRNA and protein expression levels of heparin-binding epidermal growth factor(EGF)-like growth factor(HBEGF),EGF receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),mammalian target of rapamycin(mTOR),and hypoxia-inducible factor-1α(HIF-1α) were detected by quantitative real-time polymerase chain reaction(qRT-PCR) and Western blot,respectively.Circ_0084443-overexpressing HaCaT cells and empty plasmid HaCaT cells were constructed using the lentiviral stable transfection and treated with HSYA.The expression of circ_0084443 was detected by qRT-PCR.Results:HSYA(800 μmol/L) significantly promoted HaCaT cell proliferation and migration(P<0.05or P<0.01).It also increased the mRNA and protein expression levels of HBEGF,EGFR,PI3K,AKT,mTOR and HIF-1α,and increased the phosphorylation levels of PI3K and AKT(P<0.05 or P<0.01).Furthermore,HSYA promoted HaCaT cell proliferation and migration via the HBEGF/EGFR and PI3K/AKT/m TOR signaling pathways(P<0.01).Circ_0084443 attenuated the mRNA expression levels of HBEGF,EGFR,PI3K,AKT,mTOR and HIF-1α(P<0.05).HSYA inhibited the circ_0084443 expression,further antagonized the inhibition of circ_0084443on HBEGF,EGFR,PI3K,AKT,m TOR and HIF-1α,and promoted the proliferation of circ_0084443-overexpressing HaCaT cells(P<0.05 or P<0.01).However,HSYA could not influence the inhibitory effect of circ_0084443 on HaCaT cell migration(P>0.05).Conclusion:HSYA played an accelerative role in HaCaT cell proliferation and migration,which may be attributable to activating HBEGF/EGFR and PI3K/AKT signaling pathways,and had a particular inhibitory effect on the keratinocyte negative regulator circ_0084443.
