We detected loss of heterozygosity(LOH)and microsatellite instabilities(MSI),as well as extron expression of the fragile histidine triad(FHIT)gene in gastric carcinoma(GC),in order to evaluate their association with c...We detected loss of heterozygosity(LOH)and microsatellite instabilities(MSI),as well as extron expression of the fragile histidine triad(FHIT)gene in gastric carcinoma(GC),in order to evaluate their association with clinicopathological processes in gastric carcinogenesis.LOH and MSI of the FHIT were detected by using PCR at 4 microsatellite loci:D3S 1300,D3S 4103,D3S 1481,D3S 1234 in cancer tissues from 50 patients with primary GC,with normal mucosa acting as matched controls.FHIT transcripts were detected by nested RT-PCR in 30 cases of GC and their products were sequenced.Results show that the average frequencies of LOH and MSI of the FHIT gene in GC were 32.4%and 26.4%,respectively.There was no correlation between LOH and MSI of the FHIT gene in GC and the histological characteristics of gastric carcinoma(Bormann’s or Lauren’s classification).LOH of the FHIT gene in GC was related to depth invasiveness,and its frequency in GC where serosa was penetrated was significantly higher than that in GC without serosa penetration(73.5%vs 37.5%,P<0.05).The frequency of MSI in GC without lymph node metastasis was significantly higher than that in GC with lymph node metastasis(66.7%vs 34.3%,P<0.05).Aberrant transcripts were found in 11/30 GC tissues.Sequencing analysis of the aberrant fragments found a RT-PCR product missing exons 5–7 in one case of GC,and another product missing exons 4–7.Four of 10(40.0%)cases of primary GC showed absent or decreased expression of the FHIT protein as compared to their matched normal tissues.The findings in this study suggest that LOH and MSI of FHIT gene may induce aberrant extron expression,which might play a role in gastric carcinogenesis.展开更多
基金Project supported by the Major Research Program from the State Ministry of Science and Technology(No.2012CB933301)the National Natural Science Foundation of China(No.21574068)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.YX03001)the Natural Science Foundation of Jiangsu Province(No.BM2012010)~~
基金This project was supported by the National Natural Sciences Foundation of China(Grant Nos.30600286,No.30371607).
文摘We detected loss of heterozygosity(LOH)and microsatellite instabilities(MSI),as well as extron expression of the fragile histidine triad(FHIT)gene in gastric carcinoma(GC),in order to evaluate their association with clinicopathological processes in gastric carcinogenesis.LOH and MSI of the FHIT were detected by using PCR at 4 microsatellite loci:D3S 1300,D3S 4103,D3S 1481,D3S 1234 in cancer tissues from 50 patients with primary GC,with normal mucosa acting as matched controls.FHIT transcripts were detected by nested RT-PCR in 30 cases of GC and their products were sequenced.Results show that the average frequencies of LOH and MSI of the FHIT gene in GC were 32.4%and 26.4%,respectively.There was no correlation between LOH and MSI of the FHIT gene in GC and the histological characteristics of gastric carcinoma(Bormann’s or Lauren’s classification).LOH of the FHIT gene in GC was related to depth invasiveness,and its frequency in GC where serosa was penetrated was significantly higher than that in GC without serosa penetration(73.5%vs 37.5%,P<0.05).The frequency of MSI in GC without lymph node metastasis was significantly higher than that in GC with lymph node metastasis(66.7%vs 34.3%,P<0.05).Aberrant transcripts were found in 11/30 GC tissues.Sequencing analysis of the aberrant fragments found a RT-PCR product missing exons 5–7 in one case of GC,and another product missing exons 4–7.Four of 10(40.0%)cases of primary GC showed absent or decreased expression of the FHIT protein as compared to their matched normal tissues.The findings in this study suggest that LOH and MSI of FHIT gene may induce aberrant extron expression,which might play a role in gastric carcinogenesis.
