In order to clarify the targets of Xiaoyao Wan in the treatment of depression and anxiety,the pharmacological database of traditional Chinese medicine system and the analysis platform(TCMSP)were used to search the che...In order to clarify the targets of Xiaoyao Wan in the treatment of depression and anxiety,the pharmacological database of traditional Chinese medicine system and the analysis platform(TCMSP)were used to search the chemical components and potential targets of Xiaoyao Wan.The databases of Malacards and Genecards were used to mine the targets related to depression and anxiety.With the help of Cytoscape3.2.1 software,the“Xiaoyao Wan-targets-diseases”network is constructed.The PPI network is constructed through String database.Common targets are enriched by cluster Profiler software package in R language.161 active components of Xiaoyao Wan were screened,and 247 targets related to Xiaoyao Wan were predicted(excluding repetitive targets).The related targets of depression and anxiety were 379,337 respectively.Through the intersection of Venny diagram,24 targets of Xiaoyao Wan,depression and anxiety were obtained.24 key targets were screened out by constructing the interaction network of Xiaoyao Wan,depression and anxiety.Pathway enrichment analysis showed that Xiaoyao Wan mainly acts on key targets involves D(2)dopamine receptor(DRD2),Interleukin-6(IL6),5-hydroxytryptamine receptor 2A(HTR2A),Interleukin-1 beta(IL1B),5-hydroxytryptamine receptor 3A(HTR3A),Sodium-dependent dopamine transporter(SLC6A3),D(1A)dopamine receptor(DRD1),Interleukin-10(IL10),Sodium-dependent noradrenaline transporterin(SLC6A2)and other key targets in the treatment of depression and anxiety,and participates in important pathway processes such as Serotonergic synapse,Dopaminergic synapse,Neuroactive ligand-receptor interaction and regulation of neurotransmitter levels.It is speculated that Xiaoyao Wan plays a role in the treatment of depression and anxiety by affecting neurotransmitters 5-HT and DA,participating in hypothalamic-pituitary-adrenal(HPA)and interfering in the process of inflammation.展开更多
The effects of long-day photoperiod on growth,photo synthetic fluorescence,carbon and nitrogen metabolism,and yield of Dendrocalamopsis oldhami and the compensation effects of fertilization were investigated.A complet...The effects of long-day photoperiod on growth,photo synthetic fluorescence,carbon and nitrogen metabolism,and yield of Dendrocalamopsis oldhami and the compensation effects of fertilization were investigated.A completely randomized design was used with two light factors(bamboo culms cultivated in solar greenhouse under long-day[Ls]and short-day[Ln]treatments);two organic nitrogen fertilizer levels(application of organic fertilizer[OF]and no organic fertilizer[NF]);and three nitrogen fertilizer levels(Low[N0],medium[N1]and high nitrogen[N2]).Leaf chlorophyll and fluorescence parameters(φPo,PIABS,and ETo/CSm)decreased and DIo/CSmincreased in Ls compared to Ln.Indole acetic acid(IAA)and gibberellic acid(GA3)levels decreased,whereas abscisic acid(ABA)increased.Leaf area decreased and leaf dry mass increased.The contents of carbon and nitrogen metabolism-related enzymes(nitrate reductase,glutamine synthetase,amylase,and sucrose synthase)and products(total nitrogen,organic carbon,soluble sugar,and starch)increased.Single bamboo shoot weight and diameter at breast height decreased,whereas shoot quantity and total yield increased.Fertilizer application significantly affected physiological growth and yield in the two light treatments,thus promoting carbon and nitrogen metabolism.TheφPo,PIABS,IAA,and GA3contents increased slightly,whereas ABA levels decreased.Shoot quantity,individual weight,and total yield improved.IA A,soluble sugar,and total yield to organic manure and light were lower than those of nitrogen levels(FN>FL,FO).Other indicators showed lower responses to different fertilization treatments than the light factor(FL>FN,FO).The ability of D.oldhami to alter its morphological and physiobiochemical traits and yield in response to variations in light applications may translate into high phenotypic plasticity.Fertilization significantly improved photoplasticity of D.oldhami.Under Ls,D.oldhami had high metabolic rates.was easily inhibited by light,and showed accelerated leaf senescence,and shoot quantity and total output increased.However,the quality of individual shoots decreased.Different fertilization treatments affected D.oldhami differently under the two light intensities.Ls sensitivity to nitrogen was higher.Fertilization could delay leaf dormancy and senescence under Ls treatment.Organic fertilizer addition could improve yield more effectively,with OFN1being the optimal fertilization level.展开更多
Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potenti...Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer.Methods:The data were obtained from The Cancer Genome Atlas.Kaplan-Meier analysis,CIBERSORT,gene set enrichment analysis,and gene set variation analysis were performed.The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot.Results:The expression of NSD3 was altered in pan-cancer samples.Patients with higher levels of NDS3 generally had shorter overall survival and disease-specific survival.Levels of NSD3 were positively correlated with DNA copy number variation(CNV)in pan-cancer.NSD3 expression was also associated with tumor mutation burden and microsatellite instability.The levels of immune-cell infiltration differed significantly between high and low NSD3 expression.NSD3 negatively correlated with levels of CD8+T cells.Functional enrichment analysis showed that while NSD3 expression was positively associated with several immune cell-related and histone methylation-related pathways,it was negatively correlated with cell metabolism-related,drug transport-related,and drug metabolismrelated pathways.NSD3 levels in the cell lines tested were significantly different.In U251 and NCI-H23 cells,silencing NSD3 inhibited cell proliferation and promoted apoptosis.Conclusions:NSD3 expression was changed in pan-cancer samples that was also verified in cell lines.NSD3 was associated with CNV and immune-cell infiltration.A poor prognosis was predicted in patients with high expression of NSD3.NSD3 might hence be a potential marker for predicting tumor prognosis.展开更多
Salmonella enterica has a wide diversity,with numerous serovars belonging to six different subspecies with dynamic animal-host tropism.The FimH protein is the adhesin mediating binding to various cells,and slight amin...Salmonella enterica has a wide diversity,with numerous serovars belonging to six different subspecies with dynamic animal-host tropism.The FimH protein is the adhesin mediating binding to various cells,and slight amino acid discrepancy significantly affects the adherence capacities.To date,the general function of FimH variability across dif-ferent subspecies of Salmonella enterica has not been addressed.To investigate the biological functions of FimH among the six Salmonella enterica subspecies,the present study performed several assays to determine biofilm for-mation,Caenorhabditis elegans killing,and intestinal porcine enterocyte cell IPEC-J2 adhesion by using various FimH allele mutants.In general,allelic mutations in both the lectin and pilin domains of FimH could cause changes in bind-ing affnity,such as the N79S mutation.We also observed that the N79S variation in Salmonella Dublin increased the adhesive ability of IPEC-J2 cells.Moreover,a new amino acid substitution,T260M,within the pilin domain in one subspecies llb strain beneficial to binding to cells was highlighted in this study,even though the biofilm-forming and Caenorhabditis elegans-killing abilities exhibited no significant differences in variants.Combined with point muta-tions being a natural tendency due to positive selection in harsh environments,we speculate that allelic variation T26oM probably contributes to pathoadaptive evolution in Salmonella enterica subspecies llb.展开更多
The habitat of giant pandas borders the living areas of farmers in surrounding communities. Due to the low level of economic development and resource used method, the life of community farmers depends on the natural r...The habitat of giant pandas borders the living areas of farmers in surrounding communities. Due to the low level of economic development and resource used method, the life of community farmers depends on the natural resources of the habitat of giant pandas. Analyzing the natural resource utilization of farmers in communities around the habitat of giant pandas is conducive to formulating more reasonable management policies and protecting the habitat of giant pandas more effectively. Based on the field survey data, this paper classifies the main ways of community farmers using the natural resources of the habitat of giant pandas, counts the quantity of resources used and analyzes their change trends. On this basis, this paper selects indicators, constructs a model and analyzes the main influencing factors affecting the natural resources utilization of community farmers from three aspects: the individual characteristics of household heads, the family characteristics of community farmers and the residence characteristics of community farmers.展开更多
Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the is...Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the issue of polyester plastic pollution.Therefore,we explored the interaction between PETase and the substrate(a dimer of the PET monomer ethylene terephthalate,2PET),using a model of PETase and its substrate.In this study,we focused on six key residues around the substrate-binding groove in order to create novel high-efficiency PETase mutants through protein engineering.These PETase mutants were designed and tested.The enzymatic activities of the R61A,L88F,and I179F mutants,which were obtained with a rapid cell-free screening system,exhibited 1.4 fold,2.1 fold,and 2.5 fold increases,respectively,in comparison with wild-type PETase.The I179F mutant showed the highest activity,with the degradation rate of a PET film reaching 22.5 mg perμmol·L^-1 PETase per day.Thus,this study has created enhanced artificial PETase enzymes through the rational protein engineering of key hydrophobic sites,and has further illustrated the potential of biodegradable plastics.展开更多
EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several ...EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several deep photometric surveys and there were about 40 785 EW-type binary systems listed in the international variable star index(VSX)by 2017 March 13.7938 of them were observed with LAMOST by 2016 November 30 and their spectral types were identified.Stellar atmospheric parameters of 5363 EW-type binary stars were determined based on good spectroscopic observations.In the paper,those EWs are cataloged and their properties are analyzed.The distributions of orbital period(P),effective temperature(T),gravitational acceleration(log(g)),metallicity([Fe/H])and radial velocity(RV)are presented for these observed EW-type systems.It is shown that about 80.6% of sample stars have metallicity below zero,indicating that EW-type systems are old stellar populations.This is in agreement with the conclusion that EW binaries are formed from moderately close binaries through angular momentum loss via magnetic braking that takes a few hundred million to a few billion years.The unusually high metallicities of a few percent of EWs may be caused by contamination of material from the evolution of unseen neutron stars or black holes in the systems.The correlations between orbital period and effective temperature,gravitational acceleration and metallicity are presented and their scatters are mainly caused by(i)the presence of third bodies and(ii)sometimes wrongly determined periods.It is shown that some EWs contain evolved component stars and the physical properties of EWs mainly depend on their orbital periods.It is found that extremely short-period EWs may be older than their long-period cousins because they have lower metallicities.This reveals that they have a longer timescale of pre-contact evolution and their formation and evolution are mainly driven by angular momentum loss via magnetic braking.展开更多
Wave energy is one of the most abundant renewable clean energy sources,and has been widely studied because of its advantages of continuity and low seasonal variation.However,its low capture efficiency and narrow captu...Wave energy is one of the most abundant renewable clean energy sources,and has been widely studied because of its advantages of continuity and low seasonal variation.However,its low capture efficiency and narrow capture frequency bandwidth are still technical bottlenecks that restrict the commercial application of wave energy converters(WECs).In recent years,using a nonlinear stiffness mechanism(NSM)for passive control has provided a new way to solve these technical bottlenecks.This literature review focuses on the research performed on the use of nonlinear mechanisms in wave energy device utilization,including the conceptual design of a mechanism,hydrodynamic models,dynamic characteristics,response mechanisms,and some examples of experimental verification.Finally,future research directions are discussed and recommended.展开更多
基金Key discipline project of traditional Chinese medicine pharmaceutical engineering of Shanxi provincial administration of traditional Chinese medicine(2017)Discipline innovation team project of Shanxi university of Chinese medicine(No.2019-YL11)。
文摘In order to clarify the targets of Xiaoyao Wan in the treatment of depression and anxiety,the pharmacological database of traditional Chinese medicine system and the analysis platform(TCMSP)were used to search the chemical components and potential targets of Xiaoyao Wan.The databases of Malacards and Genecards were used to mine the targets related to depression and anxiety.With the help of Cytoscape3.2.1 software,the“Xiaoyao Wan-targets-diseases”network is constructed.The PPI network is constructed through String database.Common targets are enriched by cluster Profiler software package in R language.161 active components of Xiaoyao Wan were screened,and 247 targets related to Xiaoyao Wan were predicted(excluding repetitive targets).The related targets of depression and anxiety were 379,337 respectively.Through the intersection of Venny diagram,24 targets of Xiaoyao Wan,depression and anxiety were obtained.24 key targets were screened out by constructing the interaction network of Xiaoyao Wan,depression and anxiety.Pathway enrichment analysis showed that Xiaoyao Wan mainly acts on key targets involves D(2)dopamine receptor(DRD2),Interleukin-6(IL6),5-hydroxytryptamine receptor 2A(HTR2A),Interleukin-1 beta(IL1B),5-hydroxytryptamine receptor 3A(HTR3A),Sodium-dependent dopamine transporter(SLC6A3),D(1A)dopamine receptor(DRD1),Interleukin-10(IL10),Sodium-dependent noradrenaline transporterin(SLC6A2)and other key targets in the treatment of depression and anxiety,and participates in important pathway processes such as Serotonergic synapse,Dopaminergic synapse,Neuroactive ligand-receptor interaction and regulation of neurotransmitter levels.It is speculated that Xiaoyao Wan plays a role in the treatment of depression and anxiety by affecting neurotransmitters 5-HT and DA,participating in hypothalamic-pituitary-adrenal(HPA)and interfering in the process of inflammation.
基金Basic Scientific Research Funding of the International Center for Bamboo and Rattan(1632020023)。
文摘The effects of long-day photoperiod on growth,photo synthetic fluorescence,carbon and nitrogen metabolism,and yield of Dendrocalamopsis oldhami and the compensation effects of fertilization were investigated.A completely randomized design was used with two light factors(bamboo culms cultivated in solar greenhouse under long-day[Ls]and short-day[Ln]treatments);two organic nitrogen fertilizer levels(application of organic fertilizer[OF]and no organic fertilizer[NF]);and three nitrogen fertilizer levels(Low[N0],medium[N1]and high nitrogen[N2]).Leaf chlorophyll and fluorescence parameters(φPo,PIABS,and ETo/CSm)decreased and DIo/CSmincreased in Ls compared to Ln.Indole acetic acid(IAA)and gibberellic acid(GA3)levels decreased,whereas abscisic acid(ABA)increased.Leaf area decreased and leaf dry mass increased.The contents of carbon and nitrogen metabolism-related enzymes(nitrate reductase,glutamine synthetase,amylase,and sucrose synthase)and products(total nitrogen,organic carbon,soluble sugar,and starch)increased.Single bamboo shoot weight and diameter at breast height decreased,whereas shoot quantity and total yield increased.Fertilizer application significantly affected physiological growth and yield in the two light treatments,thus promoting carbon and nitrogen metabolism.TheφPo,PIABS,IAA,and GA3contents increased slightly,whereas ABA levels decreased.Shoot quantity,individual weight,and total yield improved.IA A,soluble sugar,and total yield to organic manure and light were lower than those of nitrogen levels(FN>FL,FO).Other indicators showed lower responses to different fertilization treatments than the light factor(FL>FN,FO).The ability of D.oldhami to alter its morphological and physiobiochemical traits and yield in response to variations in light applications may translate into high phenotypic plasticity.Fertilization significantly improved photoplasticity of D.oldhami.Under Ls,D.oldhami had high metabolic rates.was easily inhibited by light,and showed accelerated leaf senescence,and shoot quantity and total output increased.However,the quality of individual shoots decreased.Different fertilization treatments affected D.oldhami differently under the two light intensities.Ls sensitivity to nitrogen was higher.Fertilization could delay leaf dormancy and senescence under Ls treatment.Organic fertilizer addition could improve yield more effectively,with OFN1being the optimal fertilization level.
基金supported in part by the National Natural Science Foundation of China(Grant No.81703005)the Natural Science Foundation of Hunan Province(Grant Nos.2017JJ3195,2018JJ3317)the Key Research and Development Projects of Hunan Province(Grant No.2018SK2120).
文摘Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer.Methods:The data were obtained from The Cancer Genome Atlas.Kaplan-Meier analysis,CIBERSORT,gene set enrichment analysis,and gene set variation analysis were performed.The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot.Results:The expression of NSD3 was altered in pan-cancer samples.Patients with higher levels of NDS3 generally had shorter overall survival and disease-specific survival.Levels of NSD3 were positively correlated with DNA copy number variation(CNV)in pan-cancer.NSD3 expression was also associated with tumor mutation burden and microsatellite instability.The levels of immune-cell infiltration differed significantly between high and low NSD3 expression.NSD3 negatively correlated with levels of CD8+T cells.Functional enrichment analysis showed that while NSD3 expression was positively associated with several immune cell-related and histone methylation-related pathways,it was negatively correlated with cell metabolism-related,drug transport-related,and drug metabolismrelated pathways.NSD3 levels in the cell lines tested were significantly different.In U251 and NCI-H23 cells,silencing NSD3 inhibited cell proliferation and promoted apoptosis.Conclusions:NSD3 expression was changed in pan-cancer samples that was also verified in cell lines.NSD3 was associated with CNV and immune-cell infiltration.A poor prognosis was predicted in patients with high expression of NSD3.NSD3 might hence be a potential marker for predicting tumor prognosis.
基金supported by the National Program on Key Research Project of China(2022YFC2604201)well as the European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No.861917-SAFFl,Zhejiang Provincial Key R&D Program of China(2023C03045)+2 种基金Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(2021JJLH0083)Key Research and Development Program of Hangzhou(202203A08)District-level project for high-level innovative and entrepreneurial talents of"Zijinshan Talents Gaochun Plan"(202100677).
文摘Salmonella enterica has a wide diversity,with numerous serovars belonging to six different subspecies with dynamic animal-host tropism.The FimH protein is the adhesin mediating binding to various cells,and slight amino acid discrepancy significantly affects the adherence capacities.To date,the general function of FimH variability across dif-ferent subspecies of Salmonella enterica has not been addressed.To investigate the biological functions of FimH among the six Salmonella enterica subspecies,the present study performed several assays to determine biofilm for-mation,Caenorhabditis elegans killing,and intestinal porcine enterocyte cell IPEC-J2 adhesion by using various FimH allele mutants.In general,allelic mutations in both the lectin and pilin domains of FimH could cause changes in bind-ing affnity,such as the N79S mutation.We also observed that the N79S variation in Salmonella Dublin increased the adhesive ability of IPEC-J2 cells.Moreover,a new amino acid substitution,T260M,within the pilin domain in one subspecies llb strain beneficial to binding to cells was highlighted in this study,even though the biofilm-forming and Caenorhabditis elegans-killing abilities exhibited no significant differences in variants.Combined with point muta-tions being a natural tendency due to positive selection in harsh environments,we speculate that allelic variation T26oM probably contributes to pathoadaptive evolution in Salmonella enterica subspecies llb.
文摘The habitat of giant pandas borders the living areas of farmers in surrounding communities. Due to the low level of economic development and resource used method, the life of community farmers depends on the natural resources of the habitat of giant pandas. Analyzing the natural resource utilization of farmers in communities around the habitat of giant pandas is conducive to formulating more reasonable management policies and protecting the habitat of giant pandas more effectively. Based on the field survey data, this paper classifies the main ways of community farmers using the natural resources of the habitat of giant pandas, counts the quantity of resources used and analyzes their change trends. On this basis, this paper selects indicators, constructs a model and analyzes the main influencing factors affecting the natural resources utilization of community farmers from three aspects: the individual characteristics of household heads, the family characteristics of community farmers and the residence characteristics of community farmers.
基金the Key Research and Development Program of Hunan province(2020WK2008)the Talent Scholars of Hunan Province for the Innovation of Science and Technology(2020RC5002)the Natural Science Foundation of Hunan Province(2021JJ31130,2020JJ5903),China.
基金supported by the National Natural Science Foundation of China(51572253,21771171)Scientific Research Grant of Hefei National Synchrotron Radiation Laboratory(UN2017LHJJ)+1 种基金the Fundamental Research Funds for the Central Universitiescooperation between NSFC and Netherlands Organization for Scientific Research(51561135011)~~
基金National Program on Key Basic Research Project by the Ministry of Science and Technology of China(2014CB745100)the National Natural Science Foundation of China(21676190 and 21621004)the Innovative Talents and Platform Program of Tianjin(16PTGCCX00140 and 16PTSYJC00050).
文摘Poly(ethylene terephthalate)hydrolase(PETase)from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate)(PET)at room temperature,and is thus regarded as a potential tool to solve the issue of polyester plastic pollution.Therefore,we explored the interaction between PETase and the substrate(a dimer of the PET monomer ethylene terephthalate,2PET),using a model of PETase and its substrate.In this study,we focused on six key residues around the substrate-binding groove in order to create novel high-efficiency PETase mutants through protein engineering.These PETase mutants were designed and tested.The enzymatic activities of the R61A,L88F,and I179F mutants,which were obtained with a rapid cell-free screening system,exhibited 1.4 fold,2.1 fold,and 2.5 fold increases,respectively,in comparison with wild-type PETase.The I179F mutant showed the highest activity,with the degradation rate of a PET film reaching 22.5 mg perμmol·L^-1 PETase per day.Thus,this study has created enhanced artificial PETase enzymes through the rational protein engineering of key hydrophobic sites,and has further illustrated the potential of biodegradable plastics.
基金supported by the National Natural Science Foundation of China (No. 11325315)National Major Scientific Project built by the Chinese Academy of SciencesFunding for the project has been provided by the National Development and Reform Commission
文摘EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several deep photometric surveys and there were about 40 785 EW-type binary systems listed in the international variable star index(VSX)by 2017 March 13.7938 of them were observed with LAMOST by 2016 November 30 and their spectral types were identified.Stellar atmospheric parameters of 5363 EW-type binary stars were determined based on good spectroscopic observations.In the paper,those EWs are cataloged and their properties are analyzed.The distributions of orbital period(P),effective temperature(T),gravitational acceleration(log(g)),metallicity([Fe/H])and radial velocity(RV)are presented for these observed EW-type systems.It is shown that about 80.6% of sample stars have metallicity below zero,indicating that EW-type systems are old stellar populations.This is in agreement with the conclusion that EW binaries are formed from moderately close binaries through angular momentum loss via magnetic braking that takes a few hundred million to a few billion years.The unusually high metallicities of a few percent of EWs may be caused by contamination of material from the evolution of unseen neutron stars or black holes in the systems.The correlations between orbital period and effective temperature,gravitational acceleration and metallicity are presented and their scatters are mainly caused by(i)the presence of third bodies and(ii)sometimes wrongly determined periods.It is shown that some EWs contain evolved component stars and the physical properties of EWs mainly depend on their orbital periods.It is found that extremely short-period EWs may be older than their long-period cousins because they have lower metallicities.This reveals that they have a longer timescale of pre-contact evolution and their formation and evolution are mainly driven by angular momentum loss via magnetic braking.
基金the State Key Laboratory of Coastal and Offshore Engineering of China(No.LP2019)the 2020 Research Program of Sanya Yazhou Bay Science and Technology City of China(No.SKJC-2020-01-006)+1 种基金the Hainan Provincial Natural Science Foundation of China(No.520QN290)the High-tech Ship Research Projects Sponsored by MIIT of China(No.[2019]357)。
文摘Wave energy is one of the most abundant renewable clean energy sources,and has been widely studied because of its advantages of continuity and low seasonal variation.However,its low capture efficiency and narrow capture frequency bandwidth are still technical bottlenecks that restrict the commercial application of wave energy converters(WECs).In recent years,using a nonlinear stiffness mechanism(NSM)for passive control has provided a new way to solve these technical bottlenecks.This literature review focuses on the research performed on the use of nonlinear mechanisms in wave energy device utilization,including the conceptual design of a mechanism,hydrodynamic models,dynamic characteristics,response mechanisms,and some examples of experimental verification.Finally,future research directions are discussed and recommended.