The role of AFAP1-AS1 in mitotic catastrophe and metastasis of triple-negative breast cancer cells by activating the PLK1 signaling pathway

Triple-negative breast cancer(TNBC)is characterized by fast growth,high metastasis,high invasion,and a lack of therapeutic targets.Mitosis and metastasis of TNBC cells are two important biological behaviors in TNBC malignant progression.It is well known that the long noncoding RNA AFAP1-AS1 plays a crucial role in various tumors,but whether AFAP1-AS1 is involved in the mitosis of TNBC cells remains unknown.In this study,we investigated the functional mechanism of AFAP1-AS1 in targeting Polo-like Kinase 1(PLK1)activation and participating in mitosis of TNBC cells.We detected the expression of AFAP1-AS1 in the TNBC patient cohort and primary cells by in situ hybridization(ISH),northern blot,fluorescent in situ hybridization(FISH)and cell nucleus/cytoplasm RNA fraction isolation.High AFAP1-AS1 expression was negatively correlated with overall survival(OS),disease-free survival(DFS),metastasis-free survival(MFS)and recurrence-free survival(RFS)in TNBC patients.We explored the function of AFAP1-AS1 by transwell,apoptosis,immunofluorescence(IF)and patient-derived xenograft(PDX)models in vitro and in vivo.We found that AFAP1-AS1 promoted TNBC primary cell survival by inhibiting mitotic catastrophe and increased TNBC primary cell growth,migration and invasion.Mechanistically,AFAP1-AS1 activated phosphorylation of the mitosis-associated kinase PLK1 protein.Elevated levels of AFAP1-AS1 in TNBC primary cells increased PLK1 pathway downstream gene expression,such as CDC25C,CDK1,BUB1 and TTK.More importantly,AFAP1-AS1 increased lung metastases in a mouse metastasis model.Taken together,AFAP1-AS1 functions as an oncogene that activates the PLK1 signaling pathway.AFAP1-AS1 could be used as a potential prognostic marker and therapeutic target for TNBC.

Analyzing the Design and Promotion of Ideological and Political Content in Online Finance and Economics Courses

In the context of the new media age,the emergence and development of finance and economics courses along with online course resources have greatly enriched the presentation of teaching resources in colleges and universities.However,subjected to the flawed design and poor promotion of ideological and political contents,which are less popular,online finance and economics courses often do not reflect the meaning well or maximize the application of the courses’ideological and political reform.This paper explores the dilemma and reasons for the promotion of the courses’ideological and political content,in hope to provide suggestions for the design and promotion of ideological and political content in online finance and economics courses in colleges and universities.

Status of Finance and Economics Students’Identity with the Civics of their Major Courses and the Path to Enhance the Teaching Effect

Under the requirements of curriculum education in the new era,the need to integrate professional courses in the Civic Education has become more urgent.In this paper,Attention,Relevance,Confidence,and Satisfaction(ARCS)motivation model from the perspective of students is used to explore the problems in the implementation of Civic Education in accounting courses from the perspective of students’learning needs and motivation as well as the essential needs of Civic Education in the curriculum.We also propose countermeasures and suggestions to meet the requirements of teachers and colleges and universities,to help in building a“student-centered”teaching mode of curriculum thinking and government.

A Study of the Impact of Fiscal Decentralization on the Efficiency of Public Health Expenditure

In this present-day global pandemic that has not been completely resolved,health is a major concern among people,and correspondingly,people are demanding higher standards for public health products and services provided by the government.In this paper,we measure the technical efficiency of public health expenditure in each province by using the data envelopment analysis(DEA)model,and examine the impact of decentralization on the efficiency of public health expenditure under the fiscal decentralization system using the panel data from 31 provinces from 2012-2019 in a panel model subject to fixed effects.