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肠出血性大肠杆菌O157多重PCR检测方法的建立 被引量:4
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作者 张昕杨 李火明 +2 位作者 夏颖 李干武 蔡文通 《中国预防兽医学报》 CAS CSCD 北大核心 2020年第4期360-365,共6页
为了建立肠出血性大肠杆菌(EHEC)O157的多重PCR检测方法,本研究选取调控基因fusR与n5512,联合经典的rfbE、stx1和stx2基因,共同作为靶基因,通过对引物浓度等参数优化,建立了稳定性好的检测EHEC O157的多重PCR方法。并检测了该方法的特... 为了建立肠出血性大肠杆菌(EHEC)O157的多重PCR检测方法,本研究选取调控基因fusR与n5512,联合经典的rfbE、stx1和stx2基因,共同作为靶基因,通过对引物浓度等参数优化,建立了稳定性好的检测EHEC O157的多重PCR方法。并检测了该方法的特异性与敏感性,结果显示,该方法对非O157的产志贺毒素的大肠杆菌、肠致病性大肠杆菌(EPEC)、沙门氏菌、假结核耶尔森菌等检测均为阴性,特异性强;对EHEC基因组DNA的检测下限约为2.27×10-2ng/μL,对EHEC CFU的检测下限约为104cfu/mL。对人工感染菌的样品进行检测,增菌前与增菌后的检测限分别为:饲料,4×103cfu/g和4×10-4cfu/g;土壤,4×103cfu/g和4×10-2cfu/g;牛肉,8×10-1cfu/g和8×10-3cfu/g;废水,4×103cfu/g和4×10-3cfu/g。该方法还能检出感染了EHEC O157小鼠的粪便。综上,本研究建立了一种优化的EHEC O157多重PCR检测方法,为动物养殖与肉品加工过程中EHEC O157的检测提供了便捷、灵敏、可靠的技术手段。 展开更多
关键词 肠出血性大肠杆菌O157 多重PCR 检测
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黄酒曲酵母的筛选和耐受性研究 被引量:2
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作者 巩园园 毛志海 +3 位作者 王奕芳 夏瑛 陈茂彬 方尚玲 《酿酒科技》 2020年第3期93-99,共7页
在酿酒过程中,酵母菌株的优劣直接影响黄酒的酒精含量和风味特征。为筛选到发酵能力强、产香强、繁殖快和耐受性强的酿酒用功能性酵母,从各种酒曲中分离纯化的13株酵母菌通过TTC法初筛和复筛得到Y-3、Y-7、Y-103株酵母菌。通过对Y-3、Y-... 在酿酒过程中,酵母菌株的优劣直接影响黄酒的酒精含量和风味特征。为筛选到发酵能力强、产香强、繁殖快和耐受性强的酿酒用功能性酵母,从各种酒曲中分离纯化的13株酵母菌通过TTC法初筛和复筛得到Y-3、Y-7、Y-103株酵母菌。通过对Y-3、Y-7、Y-10进行耐乙醇能力、耐pH能力、耐温度、耐渗透压分析,筛选出1株发酵力强、产风味物质含量较高的、耐受性较好的酵母菌株Y-10。将Y-3、Y-7、Y-10应用到黄酒生产试验中,结果表明,筛选得到的Y-10酵母在酿造的黄酒中风味物质的种类多于8种;风味物质的含量高于其他两种,高达329.87 mg/mL。由此可见,在黄酒酿造过程中,Y-10在提高黄酒的酒精含量以及增强其风味物质种类与含量方面有不可估量的应用前景。 展开更多
关键词 酵母 酒精发酵 气相色谱(GC) 耐受性 TTC法
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Analysis and compare of location technologies supporting LBS 被引量:1
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作者 CAOHai-bing xiaying +1 位作者 GEJun-wei BAEHae-young 《重庆邮电学院学报(自然科学版)》 2004年第5期42-45,共4页
Location technology is the key part of location based service. Different technologies have different accuracy, modifying to network and cost of operating. Also these technologies have limitation to network. This paper... Location technology is the key part of location based service. Different technologies have different accuracy, modifying to network and cost of operating. Also these technologies have limitation to network. This paper compares existing popular location technologies and gives some suggestions about choosing of location technologies in GSM and CDMA systems. 展开更多
关键词 LBS 定位技术 GPS 单元号 移动通讯
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Design and analysis of generic LBS application developing platform 被引量:1
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作者 xiaying GEJun-wei BAEHae-young 《重庆邮电学院学报(自然科学版)》 2004年第5期58-60,共3页
Location based services is promising due to its novel working style and contents. A software platform is proposed to provide application programs of typical location based services and support new applications develop... Location based services is promising due to its novel working style and contents. A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system. 展开更多
关键词 LBS-ADP 定位信息 移动网络系统 移动通信
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Hybrid location determination technology within urban and indoor environment based on Cell-ID and path loss
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作者 HUSheng LEIJian-jun +2 位作者 xiaying GEJun-wei BAEHae-young 《重庆邮电学院学报(自然科学版)》 2004年第5期46-49,共4页
The location determination technology based on simple delay evaluations or GPS is not accurate enough or even impossible in urban and indoor environments due to the multi path propagation.To enhance the location accur... The location determination technology based on simple delay evaluations or GPS is not accurate enough or even impossible in urban and indoor environments due to the multi path propagation.To enhance the location accuracy and reduce the operation cost within these environments, this paper proposes a novel hybrid location determination technology which combines CELL ID with the database correlation method. The proposed method generate the prediction database of path loss according to CELL ID, and after the computation the smallest squared error of measured path loss and the prediction path loss, the location of the mobile terminal is decided by the coordinates of the best matching matrix entry. 展开更多
关键词 混合定位决定技术 GPS CELL-ID 基础环境
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中国区域经济发展与政策体系演化——基于动力视角的三维分析框架 被引量:3
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作者 夏添 夏迎 +1 位作者 刘晓宇 孙久文 《地理学报》 EI CSCD 北大核心 2023年第8期1904-1919,共16页
当前中国宏观经济虽已初步实现发展目标,但仍深受外部环境和自身结构影响。回顾历史,中国区域经济从近代萌芽和起步,到1949年中华人民共和国成立后才形成发展模式,区域发展由自发转向使动阶段。对比3次区域经济实践后,本文发现区域发展... 当前中国宏观经济虽已初步实现发展目标,但仍深受外部环境和自身结构影响。回顾历史,中国区域经济从近代萌芽和起步,到1949年中华人民共和国成立后才形成发展模式,区域发展由自发转向使动阶段。对比3次区域经济实践后,本文发现区域发展战略与区域发展阶段相对应,并依次形成沿海与内陆、三大地带、四大板块的发展格局。本文进一步通过梳理文献和历史事实,确定了中国区域经济发展的历史起点、划分标准、划分方案,总结出区域发展中的3条主线和3对矛盾,即市场化改革中的政府与市场关系、经济地带分化中的沿海与内陆关系和城镇化历程中城市与农村的关系。因而将中国区域发展的时空过程构建成三维模型,并基于经济体系现代化视角提出新的区域经济历史阶段:工业经济、特区经济、地区经济、区域经济和城市网络经济。最后,以城镇化为视角,本文提出不同城镇化水平与阶段下区域政策依次应该为产业政策、要素政策和财税政策。 展开更多
关键词 政策体系演化 区域发展阶段 区域协调发展 新型城镇化 中国
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A Conflict Detection Scheme for Concurrency Control of R-tree 被引量:1
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作者 xiaying PENGDa-qin 《The Journal of China Universities of Posts and Telecommunications》 EI CSCD 2003年第1期49-54,共6页
Despite extensive research on R-trees, most of the proposed schemes have notbeen integrated into existing DBMS owing to the lack of protocols of concurrency control. R-linktree is an acceptable data structure to deal ... Despite extensive research on R-trees, most of the proposed schemes have notbeen integrated into existing DBMS owing to the lack of protocols of concurrency control. R-linktree is an acceptable data structure to deal with this issue, but problems like phantom still exist.In this paper, we focus on a conflict detection scheme based on R-link tree for completeconcurrency control. An in-memory operation control list is designed to suspend conflictingoperations . The main features of this approach are (1) it can be implemented easily and does notneed any extra information ; (2) No deadlocks are involved in locking scheme ; (3) Non-conflictingoperations are not restricted ; and (4) Phantom problems in R-link tree are avoided throughbeforehand predication . The experiment results show that this scheme is correct and gains bettersystem performance. 展开更多
关键词 concurrency control R-link tree phantom problem conflict detection
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