Objective To investigate the effects of low dose Oxymatrine(OMT)on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE.Methods CFDA-SE staining and flow cytometry were used t...Objective To investigate the effects of low dose Oxymatrine(OMT)on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE.Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT.Then,related software was used to analyze the effects of OMT on mouse lymphocyte proliferation.Results After cultured for 48 h,CFSE fluorescence could be detected by cytometer,filial generation peaks did not appear in control group,which indicated that lymphocytes did not proliferate.Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group.In groups with Con A and OMT treated,Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only.This indicated OMT obviously promote lymphocyte proliferation.After cultured for 72 h,the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h.Analyzed with CELLQuest software,it is shown that OMT could promote lymphocyte proliferation in 16,8,4 and 2μg/mL respectively.Conclusions 1)CFDA-SEdyeing and flow cytometer were both reliable tools to analyze lymphocyte proliferation;2)lower dosage of OMTcould promote the proliferation of lymphocyte as a immunopotentiator.展开更多
文摘Objective To investigate the effects of low dose Oxymatrine(OMT)on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE.Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT.Then,related software was used to analyze the effects of OMT on mouse lymphocyte proliferation.Results After cultured for 48 h,CFSE fluorescence could be detected by cytometer,filial generation peaks did not appear in control group,which indicated that lymphocytes did not proliferate.Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group.In groups with Con A and OMT treated,Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only.This indicated OMT obviously promote lymphocyte proliferation.After cultured for 72 h,the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h.Analyzed with CELLQuest software,it is shown that OMT could promote lymphocyte proliferation in 16,8,4 and 2μg/mL respectively.Conclusions 1)CFDA-SEdyeing and flow cytometer were both reliable tools to analyze lymphocyte proliferation;2)lower dosage of OMTcould promote the proliferation of lymphocyte as a immunopotentiator.
