Myostatin(MSTN) is a negative regulator of skeletal muscle growth and development. The skeletal muscle in MSTN^(-/-)mice is significantly hypertrophied, with muscle fiber type II increasing significantly while muscle ...Myostatin(MSTN) is a negative regulator of skeletal muscle growth and development. The skeletal muscle in MSTN^(-/-)mice is significantly hypertrophied, with muscle fiber type II increasing significantly while muscle fiber type I decreasing.However, it is still not clear how the skeletal muscle types change in MSTN^(-/-)pigs, and how the mechanism for MSTN regulates fiber types, especially in large animals like pigs. This study conducted a comprehensive analysis of the composition of skeletal muscle fibers in MSTN^(-/-)pigs produced in our laboratory. It was observed that, compared with wild-type(WT) pigs, both the total mass of skeletal muscle and type IIb muscle fibers increased significantly(P<0.01),while the type I and type IIa muscle fibers decreased significantly(P<0.01), in MSTN^(-/-)Meishan pigs. In addition, to explore the influence of MSTN on muscle fiber type and its regulation mechanism in the embryonic stage, this study selected a few genes(Myf5, Mef2 d, MyoD and Six1) associated with muscle fiber type and validated their expression by quantitative RT-PCR. Herein, it was found that Myh7, Myh2, Myh4 and Myh1 can be detected in the skeletal muscle of pigs at 65 days of gestation(dg). Compared with WT pigs, in MSTN^(-/-)Meishan pigs, Myh7 decreased significantly(P<0.01), while Myh4(P<0.001) and Myh1(P<0.05) increased significantly. Meanwhile, the increased expression of Myf5(P<0.05), Mef2 d(P<0.01) and Six1(P<0.05) in MSTN^(-/-)Meishan pigs suggested that MSTN should regulate the directional development of muscle fiber types in the early stage of embryonic development. Thus, at the embryonic stage, the type II muscle fibers began to increase in MSTN^(-/-)pigs. These results can provide valuable information not only for pig meat quality improvement, but also for the study of human skeletal muscle development and disease treatment.展开更多
Gastroesophageal reflux disease(GERD)is one of refractory digestive disease in clinic characterized by high prevalence,complex and recurrent symptoms.Traditional Chinese medicine(TCM)has a significant effect on the tr...Gastroesophageal reflux disease(GERD)is one of refractory digestive disease in clinic characterized by high prevalence,complex and recurrent symptoms.Traditional Chinese medicine(TCM)has a significant effect on the treatment of GERD.Spleen deficiency and damp-heat syndrome becomes one of the main syndrome patterns of GERD adapting with the changes of modern lifestyle.Although the therapy of strengthening the spleen,clearing heat and dampness is widely used in treatment of GERD,further studies still need to be conducted.The article comprehensively analyses the theoretical basis,mechanism and clinical efficacy of the therapy in order to provide more ideas for clinical treatment.展开更多
目的:筛选女贞子-黄芪药对抗癌治疗的主要活性成分,预测活性成分的作用靶点,建立药物成分-活性成分-作用靶点网络,进一步探讨女贞子-黄芪药对抗癌治疗的潜在作用机制。方法:采用中药系统药理学技术平台(TCMSP)中筛选女贞子-黄芪的潜在...目的:筛选女贞子-黄芪药对抗癌治疗的主要活性成分,预测活性成分的作用靶点,建立药物成分-活性成分-作用靶点网络,进一步探讨女贞子-黄芪药对抗癌治疗的潜在作用机制。方法:采用中药系统药理学技术平台(TCMSP)中筛选女贞子-黄芪的潜在活性成分,采用Dis Ge NET数据库与人类孟德尔遗传数据库(Online Mendelian Inheritance in Man,OMIM)预测和筛选其治疗癌症的作用靶点,采用omicshare平台(http://www. omicshare. com/)对药物靶点和疾病靶点进行匹配。借助Cytoscape软件构建"药物-活性成分-关键作用靶点"网络。采用String数据库构建蛋白质相互作用网络。采用DAVID数据库对女贞子-黄芪关键作用靶点进行生物功能及代谢通路分析。结果:通过筛选得到33个药物活性成分,共涉及203个作用靶点,与疾病靶点有关的活性成分为14个,主要通过调控ERBB2,AR,SRC,EGFR,ESR1等靶蛋白及癌症中的蛋白多糖通路、癌症通路、癌症中的微小mRNA(micro RNA)等通路等发挥抗癌治疗作用。结论:女贞子-黄芪药对对癌症的治疗作用体现了中药多成分-多靶点-多途径的特点,为阐释其抗癌治疗的作用机制与物质基础提供了科学依据。展开更多
基金supported by the National Natural Science Foundation of China(81800215)the Shandong Provincial Natural Science Foundation,China(ZR2017BH032)the National Transgenic Project of China(2014ZX08006003,2016ZX08006-001)。
文摘Myostatin(MSTN) is a negative regulator of skeletal muscle growth and development. The skeletal muscle in MSTN^(-/-)mice is significantly hypertrophied, with muscle fiber type II increasing significantly while muscle fiber type I decreasing.However, it is still not clear how the skeletal muscle types change in MSTN^(-/-)pigs, and how the mechanism for MSTN regulates fiber types, especially in large animals like pigs. This study conducted a comprehensive analysis of the composition of skeletal muscle fibers in MSTN^(-/-)pigs produced in our laboratory. It was observed that, compared with wild-type(WT) pigs, both the total mass of skeletal muscle and type IIb muscle fibers increased significantly(P<0.01),while the type I and type IIa muscle fibers decreased significantly(P<0.01), in MSTN^(-/-)Meishan pigs. In addition, to explore the influence of MSTN on muscle fiber type and its regulation mechanism in the embryonic stage, this study selected a few genes(Myf5, Mef2 d, MyoD and Six1) associated with muscle fiber type and validated their expression by quantitative RT-PCR. Herein, it was found that Myh7, Myh2, Myh4 and Myh1 can be detected in the skeletal muscle of pigs at 65 days of gestation(dg). Compared with WT pigs, in MSTN^(-/-)Meishan pigs, Myh7 decreased significantly(P<0.01), while Myh4(P<0.001) and Myh1(P<0.05) increased significantly. Meanwhile, the increased expression of Myf5(P<0.05), Mef2 d(P<0.01) and Six1(P<0.05) in MSTN^(-/-)Meishan pigs suggested that MSTN should regulate the directional development of muscle fiber types in the early stage of embryonic development. Thus, at the embryonic stage, the type II muscle fibers began to increase in MSTN^(-/-)pigs. These results can provide valuable information not only for pig meat quality improvement, but also for the study of human skeletal muscle development and disease treatment.
基金Nursery Project of Xiyuan Hospital,China Academy of Chinese Medical Sciences-Peripheral mechanism of mast cell activation of PAR2/TRPV1 pathway in NERD esophageal hypersensitivity and the intervention of Jianpi Qinghua Granules(2019XYMP-48)。
文摘Gastroesophageal reflux disease(GERD)is one of refractory digestive disease in clinic characterized by high prevalence,complex and recurrent symptoms.Traditional Chinese medicine(TCM)has a significant effect on the treatment of GERD.Spleen deficiency and damp-heat syndrome becomes one of the main syndrome patterns of GERD adapting with the changes of modern lifestyle.Although the therapy of strengthening the spleen,clearing heat and dampness is widely used in treatment of GERD,further studies still need to be conducted.The article comprehensively analyses the theoretical basis,mechanism and clinical efficacy of the therapy in order to provide more ideas for clinical treatment.
文摘目的:筛选女贞子-黄芪药对抗癌治疗的主要活性成分,预测活性成分的作用靶点,建立药物成分-活性成分-作用靶点网络,进一步探讨女贞子-黄芪药对抗癌治疗的潜在作用机制。方法:采用中药系统药理学技术平台(TCMSP)中筛选女贞子-黄芪的潜在活性成分,采用Dis Ge NET数据库与人类孟德尔遗传数据库(Online Mendelian Inheritance in Man,OMIM)预测和筛选其治疗癌症的作用靶点,采用omicshare平台(http://www. omicshare. com/)对药物靶点和疾病靶点进行匹配。借助Cytoscape软件构建"药物-活性成分-关键作用靶点"网络。采用String数据库构建蛋白质相互作用网络。采用DAVID数据库对女贞子-黄芪关键作用靶点进行生物功能及代谢通路分析。结果:通过筛选得到33个药物活性成分,共涉及203个作用靶点,与疾病靶点有关的活性成分为14个,主要通过调控ERBB2,AR,SRC,EGFR,ESR1等靶蛋白及癌症中的蛋白多糖通路、癌症通路、癌症中的微小mRNA(micro RNA)等通路等发挥抗癌治疗作用。结论:女贞子-黄芪药对对癌症的治疗作用体现了中药多成分-多靶点-多途径的特点,为阐释其抗癌治疗的作用机制与物质基础提供了科学依据。
