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利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103 被引量:4
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作者 张怀志 谢菁忠 +9 位作者 陈永兴 刘旭 王勇 闫素红 杨兆生 赵虹 王西成 贾联合 曹廷杰 刘志勇 《作物学报》 CAS CSCD 北大核心 2017年第11期1643-1649,共7页
郑麦103是一个高抗条锈病的小麦新品种,为明确其携带的抗病基因,用郑麦103与感条锈病品种农大399杂交构建分离群体,用条锈菌CYR32、CYR33和CRY34(V26)混合菌系进行田间接种和成株期抗性鉴定,对214个F2:3家系的条锈病抗性进行遗传分析,... 郑麦103是一个高抗条锈病的小麦新品种,为明确其携带的抗病基因,用郑麦103与感条锈病品种农大399杂交构建分离群体,用条锈菌CYR32、CYR33和CRY34(V26)混合菌系进行田间接种和成株期抗性鉴定,对214个F2:3家系的条锈病抗性进行遗传分析,初步确定郑麦103的抗条锈性由单个主效基因控制,定名为Yr ZM103。通过BSR-Seq技术开发了6个与Yr ZM103紧密连锁的分子标记,将Yr ZM103定位于染色体臂7BL分子标记ZM215和ZM221之间,遗传距离分别为11.8 c M和6.9 c M。利用7BL染色体上与其他已知抗条锈病基因紧密连锁的分子标记进行比较作图,发现Yr ZM103是不同于7BL末端其他抗条锈病基因的新基因。 展开更多
关键词 郑麦103 条锈病 分子标记 BSR-Seq 小麦
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QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6 被引量:1
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作者 ZHOU Sheng-hui FU Lin +12 位作者 WU Qiu-hong CHEN Jiao-jiao CHEN Yong-xing xie jing-zhong WANG Zhen-zhong WANG Guo-xin ZHANG De-yun LIANG Yong ZHANG Yan YOU Ming-shan LIANG Rong-qi HAN Jun LIU Zhi-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第2期435-444,共10页
Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative tr... Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant inbred lines (RILs) population of Yanda 1817xBeinong 6. Using phenotypic data on two locations (Beijing and Shijiazhuang, China) in two years (2012 and 2013 harvesting seasons), five QTLs, designated as QPhs.cau-3A. 1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau-4A, and QPhs.cau-6A, for PHS (GP) were detected by inclusive composite interval mapping (ICIM) (LOD≥2.5). Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29-21.65% and 4.36-5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection (MAS). 展开更多
关键词 WHEAT pre-harvest sprouting quantitative trait loci SNP TaVp-IA
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Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics 被引量:1
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作者 xie jing-zhong WANG Li-li +10 位作者 WANG Yong ZHANG Huai-zhi ZHOU Sheng-hui WU Qiu-hong CHEN Yong-xing WANG Zhen-zhong WANG Guo-xin ZHANG De-yun ZHANG Yan HU Tie-zhu LIU Zhi-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第3期540-550,共11页
Powdery mildew,caused by Blumeria graminis f.sp.tritici,is one of the most severe wheat diseases.Mining powdery mildew resistance genes in wheat cultivars and their appliance in breeding program is a promising way to ... Powdery mildew,caused by Blumeria graminis f.sp.tritici,is one of the most severe wheat diseases.Mining powdery mildew resistance genes in wheat cultivars and their appliance in breeding program is a promising way to control this disease.Genetic analysis revealed that a single dominant resistance gene named PmTm4 originated from Chinese wheat line Tangmai 4 confers resistance to prevailing isolates of B.graminis f.sp.tritici isolate E09.Detailed comparative genomics analyses helped to develop closely linked markers to PmTm4 and a fine genetic map was constructed using large F2population,in which PmTm4 was located into a 0.66-c M genetic interval.The orthologous subgenome region of PmTm4in Aegilops tauschii was identified,and two resistance gene analogs(RGA)were characterized from the corresponding sequence scaffolds of Ae.tauschii draft assembly.The closely linked markers and identified Ae.tauschii orthologs in the mapping interval provide an entry point for chromosome landing and map-based cloning of PmTm4. 展开更多
关键词 powdery mildew resistance gene PmTm4 genetic mapping comparative genomic analysis
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Comparative genetic mapping revealed powdery mildew resistance gene MlWE4 derived from wild emmer is located in same genomic region of Pm36 and Ml3D232 on chromosome 5BL 被引量:1
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作者 ZHANG Dong OUYANG Shu-hong +9 位作者 WANG Li-li CUI Yu WU Qiu-hong LIANG Yong WANG Zhen-zhong xie jing-zhong ZHANG De-yun WANG Yong CHEN Yong-xing LIU Zhi-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第4期603-609,共7页
Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat(Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. ... Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat(Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated Ml WE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of Ml WE4 was constructed, and Ml WE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes Ml WE4, Pm36 and Ml3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of Ml WE4, Pm36 and Ml3D232. 展开更多
关键词 wild emmer powdery mildew resistance gene Pm36 comparative genomics
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Molecular mapping of YrTZ2,a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii
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作者 WANG Zhen-zhong xie jing-zhong +15 位作者 GUO Li ZHANG De-yun LI Gen-qiao FANG Ti-lin CHEN Yong-xing LI Jun WU Qiu-hong LU Ping LI Miao-miao WU Hai-bin ZHANG Huai-zhi ZHANG Yan YANG Wu-yun LUO Ming-cheng Fahima Tzion LIU Zhi-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第6期1267-1275,共9页
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease that can cause severe yield losses. Identification and utilization of stripe rust resistance genes are essential for e... Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease that can cause severe yield losses. Identification and utilization of stripe rust resistance genes are essential for effective breeding against the disease. Wild emmer accession TZ-2, originally collected from Mount Hermon, Israel, confers near-immunity resistance against several prevailing Pst races in China. A set of 200 F6:7 recombinant inbred lines (RILs) derived from a cross between susceptible durum wheat cultivar Langdon and TZ-2 was used for stripe rust evaluation. Genetic analysis indicated that the stripe rust resistance of TZ-2 to Pst race CYR34 was controlled by a single dominant gene, temporarily designated YrTZ2. Through bulked segregant analysis (BSA) with SSR markers, YrTZ2 was located on chromosome arm 1BS flanked by Xwmc230 and Xgwm413 with genetic distance of 0.8 cM (distal) and 0.3 cM (proximal), respectively. By applying wheat 90K iSelect SNP genotyping assay, 11 polymorphic loci (consisting of 250 SNP markers) closely linked to YrTZ2 were identified. YrTZ2 was further delimited into a 0.8-cM genetic interval between SNP marker IWB19368 and SSR marker Xgwm413, and cosegregated with SNP marker IWB28744 (co-segregated with 28 SNP). Comparative genomics analyses revealed high level of collinearity between the YrTZ2 genomic region and the orthologous region of Aegilops tauschii 1DS. The genomic region between loci IWB19368 and IWB31649 harboring YrTZ2 is orthologous to a 24.5-Mb genomic region between AT1D0112 and AT1D0150, spanning 15 contigs on chromosome 1DS. The genetic and comparative maps of YrTZ2 rovide a framework for map-based cloning and marker-assisted selection of YrTZ2. 展开更多
关键词 Triticum dicoccoides Puccinia striformis f. sp. tritici SNP comparative genomics
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中国“国庆阅兵”报道中的隐喻集群研究 被引量:2
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作者 谢精忠 刘丽群 《新闻大学》 CSSCI 北大核心 2022年第6期75-88,122,共15页
本文基于概念隐喻理论,探讨1949年以来《人民日报》15次国庆阅兵仪式新闻报道的隐喻话语。研究发现,主流媒体基于历史文化与日常生活元素,主要采用路途隐喻等12种相对固定的隐喻类型对国庆阅兵仪式加以呈现,形成三个特定场景运用的隐喻... 本文基于概念隐喻理论,探讨1949年以来《人民日报》15次国庆阅兵仪式新闻报道的隐喻话语。研究发现,主流媒体基于历史文化与日常生活元素,主要采用路途隐喻等12种相对固定的隐喻类型对国庆阅兵仪式加以呈现,形成三个特定场景运用的隐喻集群:旅程场景集群、生活场景集群和仪式场景集群。分别发挥和强化了“勾勒社会进程”“厘定成员角色”和“营造现场氛围”的政治功能,描绘出国庆阅兵的隐喻图景。隐喻集群的变化也反映出国家发展、社会变革以及执政党的执政理念的变迁。国庆阅兵的新闻实践可以通过隐喻系统建构受众的政治认知框架,以唤起强烈的国家认同与民族情感归属。 展开更多
关键词 《人民日报》 国庆阅兵 隐喻集群 场景 政治仪式
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