Dof(DNA binding with one finger)蛋白是植物特有的一类转录因子,在植物的多种生命进程中发挥着重要作用。为解析烟草中Dof家族成员在生长发育过程中的潜在作用,本研究利用比较基因组学、进化分析、共线性分析等方法解析烟草中的Dof家...Dof(DNA binding with one finger)蛋白是植物特有的一类转录因子,在植物的多种生命进程中发挥着重要作用。为解析烟草中Dof家族成员在生长发育过程中的潜在作用,本研究利用比较基因组学、进化分析、共线性分析等方法解析烟草中的Dof家族成员的潜在生物学功能。结果表明,在烟草基因组中共鉴定到69个Dof转录因子,其Dof功能域在进化上十分保守,根据系统进化分析结果将其划分为9个亚家族。共线性分析表明,烟草中的16个Dof基因与拟南芥17个Dof基因有27个共线性基因对,同时,25个NtDof基因被检测到存在17个全基因组复制对,表明复制事件在烟草Dof基因家族的扩张中起关键作用。此外,表达模式分析表明NtDof基因的表达存在明显的组织特异性,其中NtDof05和NtDof28基因可能与根系生长发育有关。本研究对烟草基因组中的Dof转录因子进行了系统的鉴定和分析,为烟草Dof转录因子家族成员的功能研究奠定了基础。展开更多
The expression of FOAl （F-box overexpressed/oppressed ABA signaling） in different organs of Arabidopsis, and in response to ABA and NaCI, was analyzed. The expression level of FOAl is higher in the root and is lower...The expression of FOAl （F-box overexpressed/oppressed ABA signaling） in different organs of Arabidopsis, and in response to ABA and NaCI, was analyzed. The expression level of FOAl is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaC1. The phenotypes of T-DNA insertion mutant foal and FOAl overexpression lines FOAloxl and FOAlox2 were analyzed. The foal mutant exhibited a lower germination rate, shorter root length, more stomatal opening, in- creased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foal mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foal mutant. This study demonstrates that FOAl is an ABA signaling-related gene, and may play a negative role in ABA signaling.展开更多
文摘Dof(DNA binding with one finger)蛋白是植物特有的一类转录因子,在植物的多种生命进程中发挥着重要作用。为解析烟草中Dof家族成员在生长发育过程中的潜在作用,本研究利用比较基因组学、进化分析、共线性分析等方法解析烟草中的Dof家族成员的潜在生物学功能。结果表明,在烟草基因组中共鉴定到69个Dof转录因子,其Dof功能域在进化上十分保守,根据系统进化分析结果将其划分为9个亚家族。共线性分析表明,烟草中的16个Dof基因与拟南芥17个Dof基因有27个共线性基因对,同时,25个NtDof基因被检测到存在17个全基因组复制对,表明复制事件在烟草Dof基因家族的扩张中起关键作用。此外,表达模式分析表明NtDof基因的表达存在明显的组织特异性,其中NtDof05和NtDof28基因可能与根系生长发育有关。本研究对烟草基因组中的Dof转录因子进行了系统的鉴定和分析,为烟草Dof转录因子家族成员的功能研究奠定了基础。
基金supported by the National Natural Science Foundation of China(Grant No.31171176)Research Fund for the Doctoral Program of Higher Education of China(Grant No.755228001)+1 种基金Natural Science Foundation of Hunan Province(Grant No.11JJA002)the National Key Laboratory of Plant Molecular Genetics(Grant No.Y109Z711T1)
文摘The expression of FOAl （F-box overexpressed/oppressed ABA signaling） in different organs of Arabidopsis, and in response to ABA and NaCI, was analyzed. The expression level of FOAl is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaC1. The phenotypes of T-DNA insertion mutant foal and FOAl overexpression lines FOAloxl and FOAlox2 were analyzed. The foal mutant exhibited a lower germination rate, shorter root length, more stomatal opening, in- creased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foal mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foal mutant. This study demonstrates that FOAl is an ABA signaling-related gene, and may play a negative role in ABA signaling.
