目的系统评价先天性白内障患儿父母的照护体验,了解其在患儿确诊至术后康复期间的心理历程。方法检索外文数据库The Cochrane Library、PubMed、Scopus、Web of science以及中文检索库CNKI、万方、维普、CBM数据库中的质性研究,收集先...目的系统评价先天性白内障患儿父母的照护体验,了解其在患儿确诊至术后康复期间的心理历程。方法检索外文数据库The Cochrane Library、PubMed、Scopus、Web of science以及中文检索库CNKI、万方、维普、CBM数据库中的质性研究,收集先天性白内障患儿父母真实的照护体验,检索时间限定在建库至2022年12月31日。结果共纳入8篇文献,提取38个结果,归纳成9个新类别,合成3个整合结果。整合结果为:(1)父母在照护体验上存在性别、地域差异,围手术期存在明显负性情绪与压力源,多数随诊疗进展在术后康复期发展为正性心理成长。(2)家庭关系发生改变,照护者在患儿视觉缺陷和自身能力之间寻求平衡,同时对其角色不断进行调整与适应。(3)患儿父母存在自我期望与能力不符的问题,社会支持需求迫切。结论医护人员应积极关注先天性白内障患儿父母的真实照护体验,提供个性化的心理及信息支持,帮助其建立积极的观念,促进角色调整与适应,提高其生活质量。展开更多
Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of ...Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development.In this study,we investigate the effect of Scriptaid,a low-toxicity histone deacetylase inhibitor,on the developmental competence of porcine SCNT embryos.We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate(27.7%) compared with the untreated group(control)(12.2%,P<0.05).Using an immunofluorescence technique to measure the average fluorescence intensity,we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14(acH3K14).Furthermore,treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development(Oct4,Klf4 at the blastocyst stage and Nanog at the 4-cell stage).Moreover,the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages.In conclusion,these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos.展开更多
文摘目的系统评价先天性白内障患儿父母的照护体验,了解其在患儿确诊至术后康复期间的心理历程。方法检索外文数据库The Cochrane Library、PubMed、Scopus、Web of science以及中文检索库CNKI、万方、维普、CBM数据库中的质性研究,收集先天性白内障患儿父母真实的照护体验,检索时间限定在建库至2022年12月31日。结果共纳入8篇文献,提取38个结果,归纳成9个新类别,合成3个整合结果。整合结果为:(1)父母在照护体验上存在性别、地域差异,围手术期存在明显负性情绪与压力源,多数随诊疗进展在术后康复期发展为正性心理成长。(2)家庭关系发生改变,照护者在患儿视觉缺陷和自身能力之间寻求平衡,同时对其角色不断进行调整与适应。(3)患儿父母存在自我期望与能力不符的问题,社会支持需求迫切。结论医护人员应积极关注先天性白内障患儿父母的真实照护体验,提供个性化的心理及信息支持,帮助其建立积极的观念,促进角色调整与适应,提高其生活质量。
基金supported by the National Basic Research Program of China(2009CB941000)
文摘Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development.In this study,we investigate the effect of Scriptaid,a low-toxicity histone deacetylase inhibitor,on the developmental competence of porcine SCNT embryos.We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate(27.7%) compared with the untreated group(control)(12.2%,P<0.05).Using an immunofluorescence technique to measure the average fluorescence intensity,we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14(acH3K14).Furthermore,treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development(Oct4,Klf4 at the blastocyst stage and Nanog at the 4-cell stage).Moreover,the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages.In conclusion,these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos.
