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脂质体介导温敏型海藻酸/磷酸三钙/胶原Ⅰ水凝胶的性能
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作者 谢宜刚 李小丽 +2 位作者 陈昌盛 廖振华 刘伟强 《中国组织工程研究》 CAS 北大核心 2018年第14期2197-2202,共6页
背景:温敏水凝胶具有仿细胞外基质、良好生物相容性、微创可注射和原位成型等特点,在组织工程中广泛使用。目的:制备脂质体介导的温敏型海藻酸/磷酸三钙/胶原Ⅰ(Alg/TCP/Col)水凝胶,并对其进行表征。方法:通过交错融合制备包载不同浓度... 背景:温敏水凝胶具有仿细胞外基质、良好生物相容性、微创可注射和原位成型等特点,在组织工程中广泛使用。目的:制备脂质体介导的温敏型海藻酸/磷酸三钙/胶原Ⅰ(Alg/TCP/Col)水凝胶,并对其进行表征。方法:通过交错融合制备包载不同浓度钙离子的脂质体Ca-IFVs,综合考察脂质体包封率和载药率,筛选最佳钙离子浓度的脂质体,用于以下实验。将Alg/TCP/Col前体溶液(海藻酸或海藻酸/磷酸三钙前体溶液)与脂质体Ca-IFVs以不同的配比(5,10,15,20)在37℃下混合,制备温敏型水凝胶,观察水凝胶的结构、流变性、体积溶胀比及机械性能。将MC3T3-E1细胞分别接种于Alg/TCP/Col水凝胶、海藻酸水凝胶、海藻酸/磷酸三钙水凝胶表面,接种1,3,7 d后,采用共聚焦显微镜观察细胞形态。结果与结论:(1)冻干水凝胶中孔的尺寸在50-100μm范围,α-磷酸三钙的颗粒均匀地附着在海藻酸/α-磷酸三钙水凝胶的表面上;与海藻酸/α-磷酸三钙水凝胶相比,Alg/TCP/Col水凝胶是具有胶原纤维的致密聚集体;(2)Alg/TCP/Col水凝胶具有在35-39℃之间适当的相转变温度Tm;(3)随着复合材料凝胶中Ca-IFVs体积的增加,凝胶体积溶胀比增加,当将α-磷酸三钙复合物加入到海藻酸/α-磷酸三钙水凝胶中时,溶胀比略有降低;与海藻酸/α-磷酸三钙水凝胶相比,Alg/TCP/Col水凝胶的溶胀比增加;(4)在前体溶液与脂质体配比为10时,Alg/TCP/Col水凝胶、海藻酸/α-磷酸三钙水凝胶的压缩模量高于海藻酸水凝胶(P<0.05);(5)在前体溶液与脂质体配比为10时,海藻酸水凝胶表面的MC3T3-E1细胞呈圆形;海藻酸/α-磷酸三钙水凝胶中的细胞形态趋于扩展,散布在水凝胶孔表面上;在Alg/TCP/Col水凝胶表面的细胞具有应力延长形态,细胞形态趋势相似,并向凝胶内部延伸,同时数量显著增加;(6)结果表明脂质体介导的Alg/TCP/Col水凝胶,具有良好的机械性能及细胞相容性。 展开更多
关键词 水凝胶 胶原 脂质体 磷酸钙类 组织工程 组织修复 温度敏感 国家自然科学基金 生物材料
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A Cosmic-Ray Muon Hodoscope Based on Up-down THGEM Detectors 被引量:1
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作者 PANG Hong-Chao LIU Hong-Bang +7 位作者 CHEN Shi MIN Jian ZHENG Yang-Heng xie yi-gang TANG Ai-Song YANG Ya-Dan DONG Yang LI Min 《Chinese Physics Letters》 SCIE CAS CSCD 2012年第1期75-78,共4页
Thick gas electron multipliers (THGEMs) are new types of gas detectors with merits such as a high counting rate,anti-radiation,high gain,robustness and relatively low cost.We establish an up-down THGEM detector hodosc... Thick gas electron multipliers (THGEMs) are new types of gas detectors with merits such as a high counting rate,anti-radiation,high gain,robustness and relatively low cost.We establish an up-down THGEM detector hodoscope system for detecting and displaying muon tracks.Muon tracks can be well observed with the detectors and the present study lays an important technological foundation for the domestic and mass production of THGEMs. 展开更多
关键词 THGEM FOUNDATION SYSTEM
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Study of Thick Gaseous Electron Multipliers Gain Stability and Some Influencing Factors 被引量:1
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作者 ZHOU Xiao-Kang LIU Qian +13 位作者 CHEN Shi HUANG Wen-Qian WANG Bin-Long ZHANG Yong-Dong GE Dong-Sheng LIU Hong-Bang ZHENG Yang-Heng xie yi-gang DONG Yang ZHANG Qiang CHANG Jie WANG Jing LI Min ZHOU Yan 《Chinese Physics Letters》 SCIE CAS CSCD 2014年第3期47-50,共4页
The working stability of thinner-thick gaseous electron multipliers (THGEMs), which have been developed by the University of the Chinese Academy of Sciences and the Second Academy of China's Aerospace Science and I... The working stability of thinner-thick gaseous electron multipliers (THGEMs), which have been developed by the University of the Chinese Academy of Sciences and the Second Academy of China's Aerospace Science and Industry Corporation, is studied with an 8 keV x-ray on a Cu target. Gains of about 103–104 are achieved with a single board in Ar:iC4H10 (97:3). Environmental factors, such as pressure, temperature and humidity are considered. The thinner-THGEMs are shown to perform stably over two months of studies. 展开更多
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