Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly ...Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly understood.In this study,efforts were made to elucidate how MFT influences the fruit quality of Ponkan.The results showed that MFT substantially increased fruit size and elevated fruit total soluble solids in comparison with the fruit from the unthinned trees(used as control).Expression analyses demonstrated that m RNA abundance of three important sugar transporter genes,including CrSUT1,CrSTP1 and CrTMT1,was evidently elevated in the flesh of thinned fruit when compared with those of the control.In addition,MFT prominently up-regulated the transcript levels of various auxin and gibberellin(GA)biosynthesis and signaling genes,including CrYUC6,CrAUX/IAA,CrGA20ox1 and CrGA3ox1.Concurrently,the contents of endogenous IAA and GA3,measured at 90 d after fruit thinning,were notably elevated in the fruit from trees with the thinning treatment relative to the control,although no difference was detected in the two groups before the thinning manipulation.Taken together,these results indicate that manual fruit thinning could greatly improve fruit quality,which may be attributed to promoting fruit expansion due to the increased auxin levels and expediting sugar accumulation through the up-regulation of sugar transporter genes.展开更多
目的弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)预后因素尚不明确.本研究探讨TLR4、CD163和CD206在DLBCL组织表达及临床意义,并寻找新的DLBCL预后指标.方法回顾性分析2011-06-01-2017-06-30中南大学湘雅医学院附属海口医...目的弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)预后因素尚不明确.本研究探讨TLR4、CD163和CD206在DLBCL组织表达及临床意义,并寻找新的DLBCL预后指标.方法回顾性分析2011-06-01-2017-06-30中南大学湘雅医学院附属海口医院确诊的84例DLBCL患者,收集患者临床资料、治疗及预后信息,采用免疫组织化学法检测DLBCL组织中TLR4、CD163和CD206分子表达,分析其表达程度与临床病理特征及预后关系,组间比较采用χ^2检验,应用Kaplan-Meier法绘制生存曲线,Log-rank法进行曲线间单因素分析,多因素分析采用Cox回归模型.结果 TLR4蛋白表达阳性与LDH水平升高有关联,其中LDH水平升高者占TLR4蛋白表达阳性患者的58.1%(25/43),差异有统计学意义,χ^2=4.858,P=0.031.TLR4、CD163和CD206蛋白表达在患者年龄、性别、Ann Arbor分期、美国东部肿瘤协作组评分、国际预后指数评分、B症状、Hans法分型之间差异均无统计学意义,均P>0.05.Spearman秩次相关分析结果显示,在DLBCL组织中TLR4与CD163(r=0.167,P=0.190)和CD206表达(r=0.191,P=0.087)无关联.单因素分析显示,年龄(χ^2=5.192,P=0.023)、TLR4(χ^2=5.772,P=0.016)和CD206表达水平(χ^2=4.575,P=0.032)与中位无进展生存期有关联,CD206表达水平(χ^2=5.060,P=0.024)与中位总生存期有关联.多因素分析显示,TLR4(HR=1.755,P=0.019)和CD206(HR=1.638,P=0.036)是影响无进展生存期独立预后危险性因素;CD206(HR=1.668,P=0.029)是影响总生存期独立预后危险性因素.结论TLR4和CD206蛋白表达对判断DLBCL患者预后有一定临床价值,有望成为评估DLBCL预后的新指标.展开更多
目的了解2009~2018年癌症康复相关研究的研究现状、研究热点及研究前沿。方法检索2009~2018年Web of Science核心数据库中癌症康复相关文献,利用CiteSpace软件生成可视化科学知识图谱并进行分析。结果2009~2018年,Web of Science核心数...目的了解2009~2018年癌症康复相关研究的研究现状、研究热点及研究前沿。方法检索2009~2018年Web of Science核心数据库中癌症康复相关文献,利用CiteSpace软件生成可视化科学知识图谱并进行分析。结果2009~2018年,Web of Science核心数据库中共检索到癌症康复相关论文13956篇,年度发表论文数量总体呈增长趋势;《SUPPORTIVE CARE IN CANCER》是发表论文最多的期刊,美国是发表文章数量最多、最具权威性的国家,UNIVERSITY OF ALBERTA(阿尔伯塔大学)是最活跃的机构,Courneya KS是发表文献数量最多的作者;癌症研究领域热点词包括生活质量、体育活动、康复、运动、随机对照试验、前列腺癌、乳腺癌、淋巴水肿和肺癌。癌症、绝经后妇女、心血管疾病、主要结局指标、规律运动、氧化应激、社会支持、住院时间等可能是癌症康复未来潜在的研究方向。结论癌症康复研究正在经历快速发展的阶段。近10年来,癌症康复研究形成了以国家和研究机构为单位的研究群体,其中欧美国家对癌症康复的研究处于世界领先水平,美国在癌症康复领域的研究具有相当重要的地位。癌症康复领域研究热点和学术前沿包括研究疾病、研究对象和研究方法3个方面,研究疾病主要集中于前列腺癌、乳腺及肺癌及并发症,研究群体以女性居多,研究方法多采用随机对照试验。展开更多
基金supported by the Hubei Provincial Agriculture Research System,Chinathe Special Program for Technology Innovation of Hubei Province,China(2020BBA036)the Hubei Provincial Agricultural Science and Technology Innovation,China。
文摘Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly understood.In this study,efforts were made to elucidate how MFT influences the fruit quality of Ponkan.The results showed that MFT substantially increased fruit size and elevated fruit total soluble solids in comparison with the fruit from the unthinned trees(used as control).Expression analyses demonstrated that m RNA abundance of three important sugar transporter genes,including CrSUT1,CrSTP1 and CrTMT1,was evidently elevated in the flesh of thinned fruit when compared with those of the control.In addition,MFT prominently up-regulated the transcript levels of various auxin and gibberellin(GA)biosynthesis and signaling genes,including CrYUC6,CrAUX/IAA,CrGA20ox1 and CrGA3ox1.Concurrently,the contents of endogenous IAA and GA3,measured at 90 d after fruit thinning,were notably elevated in the fruit from trees with the thinning treatment relative to the control,although no difference was detected in the two groups before the thinning manipulation.Taken together,these results indicate that manual fruit thinning could greatly improve fruit quality,which may be attributed to promoting fruit expansion due to the increased auxin levels and expediting sugar accumulation through the up-regulation of sugar transporter genes.
文摘目的了解2009~2018年癌症康复相关研究的研究现状、研究热点及研究前沿。方法检索2009~2018年Web of Science核心数据库中癌症康复相关文献,利用CiteSpace软件生成可视化科学知识图谱并进行分析。结果2009~2018年,Web of Science核心数据库中共检索到癌症康复相关论文13956篇,年度发表论文数量总体呈增长趋势;《SUPPORTIVE CARE IN CANCER》是发表论文最多的期刊,美国是发表文章数量最多、最具权威性的国家,UNIVERSITY OF ALBERTA(阿尔伯塔大学)是最活跃的机构,Courneya KS是发表文献数量最多的作者;癌症研究领域热点词包括生活质量、体育活动、康复、运动、随机对照试验、前列腺癌、乳腺癌、淋巴水肿和肺癌。癌症、绝经后妇女、心血管疾病、主要结局指标、规律运动、氧化应激、社会支持、住院时间等可能是癌症康复未来潜在的研究方向。结论癌症康复研究正在经历快速发展的阶段。近10年来,癌症康复研究形成了以国家和研究机构为单位的研究群体,其中欧美国家对癌症康复的研究处于世界领先水平,美国在癌症康复领域的研究具有相当重要的地位。癌症康复领域研究热点和学术前沿包括研究疾病、研究对象和研究方法3个方面,研究疾病主要集中于前列腺癌、乳腺及肺癌及并发症,研究群体以女性居多,研究方法多采用随机对照试验。
