A metamaterial vibration isolator,termed as wave-insulating isolator,is proposed,which preserves enough load-bearing capability and offers ultra-low and broad bandgaps for greatly enhanced wave insulation.It consists ...A metamaterial vibration isolator,termed as wave-insulating isolator,is proposed,which preserves enough load-bearing capability and offers ultra-low and broad bandgaps for greatly enhanced wave insulation.It consists of plate-shaped metacells,whose symmetric and antisymmetric local resonant modes offer several low and broad mode bandgaps although the complete bandgap remains high and narrow.The bandgap mechanisms,vibration isolation properties,effects of key parameters,and robustness to complex conditions are clarified.As experimentally demonstrated,the wave-insulating isolator can improve the vibration insulation in the ranges of[50 Hz,180 Hz]and[260 Hz,400 Hz]by 15 dB and 25 dB,respectively,in contrast to the conventional isolator with the same first resonant frequency.展开更多
The violent vibration of supersonic wings threatens aircraft safety.This paper proposes the strongly nonlinear acoustic metamaterial(NAM)method to mitigate aeroelastic vibration in supersonic wing plates.We employ the...The violent vibration of supersonic wings threatens aircraft safety.This paper proposes the strongly nonlinear acoustic metamaterial(NAM)method to mitigate aeroelastic vibration in supersonic wing plates.We employ the cantilever plate to simulate the practical behavior of a wing.An aeroelastic vibration model of the NAM cantilever plate is established based on the mode superposition method and a modified third-order piston theory.The aerodynamic properties are systematically studied using both the timedomain integration and frequency-domain harmonic balance methods.While presenting the flutter and post-flutter behaviors of the NAM wing,we emphasize more on the preflutter broadband vibration that is prevalent in aircraft.The results show that the NAM method can reduce the low-frequency and broadband pre-flutter steady vibration by 50%-90%,while the post-flutter vibration is reduced by over 95%,and the critical flutter velocity is also slightly delayed.As clarified,the significant reduction arises from the bandgap,chaotic band,and nonlinear resonances of the NAM plate.The reduction effect is robust across a broad range of parameters,with optimal performance achieved with only 10%attached mass.This work offers a novel approach for reducing aeroelastic vibration in aircraft,and it expands the study of nonlinear acoustic/elastic metamaterials.展开更多
To solve the problem of low broadband multi-directional vibration control of fluid-conveying pipes,a novel metamaterial periodic structure with multi-directional wide bandgaps is proposed.First,an integrated design me...To solve the problem of low broadband multi-directional vibration control of fluid-conveying pipes,a novel metamaterial periodic structure with multi-directional wide bandgaps is proposed.First,an integrated design method is proposed for the longitudinal and transverse wave control of fluid-conveying pipes,and a novel periodic structure unit model is constructed for vibration reduction.Based on the bandgap vibration reduction mechanism of the acoustic metamaterial periodic structure,the material parameters,structural parameters,and the arrangement interval of the periodic structure unit are optimized.The finite element method(FEM)is used to predict the vibration transmission characteristics of the fluid-conveying pipe installed with the vibration reduction periodic structure.Then,the wave/spectrum element method(WSEM)and experimental test are used to verify the calculated results above.Lastly,the vibration attenuation characteristics of the structure under different conditions,such as rubber material parameters,mass ring material,and fluid-structure coupling effect,are analyzed.The results show that the structure can produce a complete bandgap of 46 Hz-75 Hz in the low-frequency band below 100 Hz,which can effectively suppress the low broadband vibration of the fluidconveying pipe.In addition,a high damping rubber material is used in the design of the periodic structure unit,which realizes the effective suppression of each formant peak of the pipe,and improves the vibration reduction effect of the fluid-conveying pipe.Meanwhile,the structure has the effect of suppressing both bending vibration and longitudinal vibration,and effectively inhibits the transmission of transverse waves and longitudinal waves in the pipe.The research results provide a reference for the application of acoustic metamaterials in the multi-directional vibration control of fluid-conveying pipes.展开更多
Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza,and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo....Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza,and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo.SmMYB36 has been found to have important regulatory functions in the synthesis of tanshinone and phenolic acid;paradoxically,its mechanism of action in S.miltiorrhiza is not clear.Here,we demonstrated that SmMYB36 functions as a promoter of tanshinones accumulation and a suppressor of phenolic acids through the generation of SmMYB36 overexpressed and chimeric SmMYB36-SRDX(EAR repressive domain)repressor hairy roots in combination with transcriptomic-metabolomic analysis.SmMYB36 directly down-regulate the key enzyme gene of primary metabolism,SmGAPC,up-regulate the tanshinones biosynthesis branch genes SmDXS2,SmGGPPS1,SmCPS1 and down-regulate the phenolic acids biosynthesis branch enzyme gene,SmRAS.Meanwhile,SmERF6,a positive regulator of tanshinone synthesis activating SmCPS1,was up-regulated and SmERF115,a positive regulator of phenolic acid biosynthesis activating SmRAS,was down-regulated.Furthermore,the seven acidic amino acids at the C-terminus of SmMYB36 are required for both self-activating domain and activation of target gene expression.As a consequence,this study contributes to reveal the potential relevance of transcription factors synergistically regulating the biosynthesis of tanshinone and phenolic acid.展开更多
In Alzheimer’s disease,the transporter P-glycoprotein is responsible for the clearance of amyloid-βin the brain.Amyloid-βcorrelates with the sphingomyelin metabolism,and sphingomyelin participates in the regulation...In Alzheimer’s disease,the transporter P-glycoprotein is responsible for the clearance of amyloid-βin the brain.Amyloid-βcorrelates with the sphingomyelin metabolism,and sphingomyelin participates in the regulation of P-glycoprotein.The amyloid cascade hypothesis describes amyloid-βas the central cause of Alzheimer’s disease neuropathology.Better understanding of the change of P-glycoprotein and sphingomyelin along with amyloid-βand their potential association in the pathological process of Alzheimer’s disease is critical.Herein,we found that the expression of P-glycoprotein in APP/PS1 mice tended to increase with age and was significantly higher at 9 and 12 months of age than that in wild-type mice at comparable age.The functionality of P-glycoprotein of APP/PS1 mice did not change with age but was significantly lower than that of wild-type mice at 12 months of age.Decreased sphingomyelin levels,increased ceramide levels,and the increased expression and activity of neutral sphingomyelinase 1 were observed in APP/PS1 mice at 9 and 12 months of age compared with the levels in wild-type mice.Similar results were observed in the Alzheimer’s disease mouse model induced by intracerebroventricular injection of amyloid-β1-42 and human cerebral microvascular endothelial cells treated with amyloid-β1-42.In human cerebral microvascular endothelial cells,neutral sphingomyelinase 1 inhibitor interfered with the changes of sphingomyelin metabolism and P-glycoprotein expression and functionality caused by amyloid-β1-42 treatment.Neutral sphingomyelinase 1 regulated the expression and functionality of P-glycoprotein and the levels of sphingomyelin and ceramide.Together,these findings indicate that neutral sphingomyelinase 1 regulates the expression and function of P-glycoprotein via the sphingomyelin/ceramide pathway.These studies may serve as new pursuits for the development of anti-Alzheimer’s disease drugs.展开更多
Social infrastructures such as dams are likely to be exposed to high risk of terrorist and military attacks,leading to increasing attentions on their vulnerability and catastrophic consequences under such events.This ...Social infrastructures such as dams are likely to be exposed to high risk of terrorist and military attacks,leading to increasing attentions on their vulnerability and catastrophic consequences under such events.This paper tries to develop advanced deep learning approaches for structural dynamic response prediction and dam health diagnosis.At first,the improved long short-term memory(LSTM)networks are proposed for data-driven structural dynamic response analysis with the data generated by a single degree of freedom(SDOF)and the finite numerical simulation,due to the unavailability of abundant practical structural response data of concrete gravity dam under blast events.Three kinds of LSTM-based models are discussed with the various cases of noise-contaminated signals,and the results prove that LSTM-based models have the potential for quick structural response estimation under blast loads.Furthermore,the damage indicators(i.e.,peak vibration velocity and domain frequency)are extracted from the predicted velocity histories,and their relationship with the dam damage status from the numerical simulation is established.This study provides a deep-learning based structural health monitoring(SHM)framework for quick assessment of dam experienced underwater explosions through blastinduced monitoring data.展开更多
Potential damage in composite structures caused by hail ice impact is an essential safety threat to the aircraft in flight.In this study,a nonlinear finite element(FE)model is developed to investigate the dynamic resp...Potential damage in composite structures caused by hail ice impact is an essential safety threat to the aircraft in flight.In this study,a nonlinear finite element(FE)model is developed to investigate the dynamic response and damage behavior of hybrid corrugated sandwich structures subjected to high velocity hail ice impact.The impact and breaking behavior of hail are described using the FE-smoothed particle hydrodynamics(FE-SPH)method.A rate-dependent progressive damage model is employed to capture the intra-laminar damage response;cohesive element and surface-based cohesive contact are implemented to predict the inter-laminar delamination and sheet/core debonding phenomena respectively.The transient processes of sandwich structure under different hail ice impact conditions are analyzed.Comparative analysis is conducted to address the influences of core shape and impact position on the impact performance of sandwich structures and the corresponding energy absorption characteristics are also revealed.展开更多
To provide a more accurate evaluation of the scale of deep underground resources,a new pressure coring system was carefully developed and its strength and safety of the engineering were verified by theoretical analyse...To provide a more accurate evaluation of the scale of deep underground resources,a new pressure coring system was carefully developed and its strength and safety of the engineering were verified by theoretical analyses and numerical simulation.The designed pressure coring system can obtain cores with length of 3 m and diameter of 50 mm at 70 MPa.The results of the ball-drop operation experiment demonstrate that differential motion assembly can effectively cut a safety pin by applying a tensile force of 4852 N,and it can lift the core tube through the center pole to complete a series of mechanical actions to seal the pressure.Additionally,by maintaining pressures at 70 MPa for 30 min,the pressure sealing capacity of the system was proven.Furthermore,a core sample with a diameter of 50 mm was obtained through a core drilling experiment and the coring performance of the pressure coring system was verified.This study can not only enrich the existing onshore coring technology but also provide a theoretical guide and design criteria for the development of similar pressure coring systems to meet the demand for deeper underground exploration.展开更多
BACKGROUND Through significant advances in the treatment of peripheral arterial occlusive disease,acute ischemia of the lower extremity is still associated with significant morbidity,limb threat and mortality.The two ...BACKGROUND Through significant advances in the treatment of peripheral arterial occlusive disease,acute ischemia of the lower extremity is still associated with significant morbidity,limb threat and mortality.The two main causes of acute ischemia in lower extremities are arterial embolism and atherosclerotic arteries.Timely recognition and treatment of acute limb ischemia in emergency situations is essential in order to minimize the duration of ischemia.AIM To investigate the application effect of angiojet thrombolysis in the treatment of acute lower extremity arterial embolization.METHODS Sixty-two patients with acute lower extremity arterial embolization admitted to our hospital from May 2018 to May 2020 were selected.Among them,the observation group(twenty-eight cases)had received angiojet thrombolysis,and the control group(thirty-four cases)had received femoral artery incision and thrombectomy.After thrombus clearance,significant residual stenosis of the lumen was combined with balloon dilation and/or stent implantation.When the thrombus removal was not satisfactory,catheter-directed thrombolysis was performed.The incidence of postoperative complications,recurrence rate and recovery of the two groups were compared.RESULTS There were no significant differences in postoperative recurrence(target vessel reconstruction rate),anklebrachial index and the incidence of postoperative complications between the two groups(P>0.05);there were statistically significant differences in postoperative pain score and postoperative rehabilitation between the two groups(P<0.05).CONCLUSION The application of angiojet in the treatment of acute lower limb artery thromboembolism disease is safe and effective,minimally invasive,quicker recovery after operation,less postoperative complications,which is more suitable for the treatment of femoral popliteal arterial thromboembolism lesions.If the thrombus removal is not satisfactory,the combination of coronary artery aspiration catheter and catheterized directed thrombolysis can be used.Balloon dilation and stent implantation can be considered for obvious lumen stenosis.展开更多
Cognitive dysfunction in Alzheimer's disease is strongly associated with a reduction in synaptic plasticity, which may be induced by oxidative stress. Testosterone is beneficial in learning and memory, although th...Cognitive dysfunction in Alzheimer's disease is strongly associated with a reduction in synaptic plasticity, which may be induced by oxidative stress. Testosterone is beneficial in learning and memory, although the underlying protective mechanism of testosterone on cognitive performance remains unclear. This study explored the protective mechanism of a subcutaneous injection of 0.75 mg testosterone on cognitive dysfunction induced by bilateral injections of amyloid beta 1–42 oligomers into the lateral ventricles of male rats. Morris water maze test results demonstrated that testosterone treatment remarkably reduced escape latency and path length in Alzheimer's disease rat models. During probe trials, testosterone administration significantly elevated the percentage of time spent in the target quadrant and the number of platform crossings. However, flutamide, an androgen receptor antagonist, inhibited the protective effect of testosterone on cognitive performance in Alzheimer's disease rat models. Nissl staining, immunohistochemistry, western blot assay, and enzyme-linked immunosorbent assay results showed that the number of intact hippocampal pyramidal cells, the dendritic spine density in the hippocampal CA1 region, the immune response and expression level of postsynaptic density protein 95 in the hippocampus, and the activities of superoxide dismutase and glutathione peroxidase were increased with testosterone treatment. In contrast, testosterone treatment reduced malondialdehyde levels. Flutamide inhibited the effects of testosterone on all of these indicators. Our data showed that the protective effect of testosterone on cognitive dysfunction in Alzheimer's disease is mediated via androgen receptors to scavenge free radicals, thereby enhancing synaptic plasticity.展开更多
Isoflurane and sevoflurane are both inhalation anesthetics,but in clinical application,sevoflurane has been considered to be less suitable for long-term anesthesia because of its catabolic compounds and potential neph...Isoflurane and sevoflurane are both inhalation anesthetics,but in clinical application,sevoflurane has been considered to be less suitable for long-term anesthesia because of its catabolic compounds and potential nephrotoxicity.Nevertheless,recent studies have shown that these two inhalation anesthetics are similar in hepatorenal toxicity,cost,and long-term anesthetic effect.Moreover,sevoflurane possibly has less cognitive impact on young mice.In this study,C57BL/6 mice aged 8–10 weeks were exposed to 1.2%isoflurane or 2.4%sevoflurane for 6 hours.Cognitive function and memory were examined in young mice using the novel object recognition,contextual fear conditioning,and cued-fear extinction tests.Western blot assay was performed to detect expression levels of D1 dopamine receptor,catechol-O-methyltransferase,phospho-glycogen synthase kinase-3β,and total glycogen synthase kinase-3βin the hippocampus.Our results show that impaired performance was not detected in mice exposed to sevoflurane during the novel object recognition test.Contextual memory impairment in the fear conditioning test was shorter in the sevoflurane group than the isoflurane group.Long-term sevoflurane exposure did not affect memory consolidation,while isoflurane led to memory consolidation and reduced retention.Downregulation of hippocampal D1 dopamine receptors and phosphorylated glycogen synthase kinase-3β/total glycogen synthase kinase-3βand upregulation of catechol-O-methyltransferase may be associated with differing memory performance after exposure to isoflurane or sevoflurane.These results confirm that sevoflurane has less effect on cognitive impairment than isoflurane,which may be related to expression of D1 dopamine receptors and catechol-O-methyltransferase and phosphorylation of glycogen synthase kinase-3βin the hippocampus.This study was approved by the Institutional Animal Care and Use Committee,Nanjing University,China on November 20,2017(approval No.20171102).展开更多
Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive dysfunction.Photoacoustic treatments including light and sound stimulation have been found to b...Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive dysfunction.Photoacoustic treatments including light and sound stimulation have been found to be effective in modulating sleep patterns and improving cognitive behavior in abnormal sleep-wake pattern experiments.In this study,we examined whether light and sound interventions could reduce sleep-wake pattern disturbances and memory deficits in a sleep rhythm disturbance model.We established a model of sleep rhythm disturbance in C57 BL/6 J mice via a sleep deprivation method involving manual cage tapping,cage jostling,and nest disturbance.We used a Mini Mitter radio transmitter device to monitor motor activity in the mice and fear conditioning tests to assess cognitive function.Our results indicated that an intervention in which the mice were exposed to blue light(40-Hz flickering frequency)for 1 hour during their subjective daytime significantly improved the 24-hour-acrophase shift and reduced the degree of memory deficit induced by sleep deprivation.However,interventions in which the mice were exposed to a 40-Hz blue light at offset time or subjective night time points,as well as 2 Hz-blue light at 3 intervention time points(subjective day time,subjective night time,and offset time points),had no positive effects on circadian rhythm shift or memory deficits.Additionally,a 2000-Hz sound intervention during subjective day time attenuated the24-hour-acrophase shift and memory decline,while 440-Hz and 4000-Hz sounds had no effect on circadian rhythms.Overall,these results demonstrate that photoacoustic treatment effectively corrected abnormal sleep-wake patterns and cognitive dysfunction associated with sleep-deprivation-induced disturbances in sleep-wake rhythm.All animal experiments were approved by the Experimental Animal Ethics Committee of Drum Tower Hospital Affiliated to the Medical College of Nanjing University,China(approval No.20171102)on November20,2017.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52241103 and 52322505)the Natural Science Fund for Distinguished Young Scholars of Hunan Province of China(No.2023JJ10055)。
文摘A metamaterial vibration isolator,termed as wave-insulating isolator,is proposed,which preserves enough load-bearing capability and offers ultra-low and broad bandgaps for greatly enhanced wave insulation.It consists of plate-shaped metacells,whose symmetric and antisymmetric local resonant modes offer several low and broad mode bandgaps although the complete bandgap remains high and narrow.The bandgap mechanisms,vibration isolation properties,effects of key parameters,and robustness to complex conditions are clarified.As experimentally demonstrated,the wave-insulating isolator can improve the vibration insulation in the ranges of[50 Hz,180 Hz]and[260 Hz,400 Hz]by 15 dB and 25 dB,respectively,in contrast to the conventional isolator with the same first resonant frequency.
基金supported by the National Natural Science Foundation of China(Nos.52241103,52322505,and 11991032)the Natural Science Foundation of Hunan Province of China(No.2023JJ10055)。
文摘The violent vibration of supersonic wings threatens aircraft safety.This paper proposes the strongly nonlinear acoustic metamaterial(NAM)method to mitigate aeroelastic vibration in supersonic wing plates.We employ the cantilever plate to simulate the practical behavior of a wing.An aeroelastic vibration model of the NAM cantilever plate is established based on the mode superposition method and a modified third-order piston theory.The aerodynamic properties are systematically studied using both the timedomain integration and frequency-domain harmonic balance methods.While presenting the flutter and post-flutter behaviors of the NAM wing,we emphasize more on the preflutter broadband vibration that is prevalent in aircraft.The results show that the NAM method can reduce the low-frequency and broadband pre-flutter steady vibration by 50%-90%,while the post-flutter vibration is reduced by over 95%,and the critical flutter velocity is also slightly delayed.As clarified,the significant reduction arises from the bandgap,chaotic band,and nonlinear resonances of the NAM plate.The reduction effect is robust across a broad range of parameters,with optimal performance achieved with only 10%attached mass.This work offers a novel approach for reducing aeroelastic vibration in aircraft,and it expands the study of nonlinear acoustic/elastic metamaterials.
基金supported by the National Natural Science Foundation of China(Nos.11991032 and 52241103)。
文摘To solve the problem of low broadband multi-directional vibration control of fluid-conveying pipes,a novel metamaterial periodic structure with multi-directional wide bandgaps is proposed.First,an integrated design method is proposed for the longitudinal and transverse wave control of fluid-conveying pipes,and a novel periodic structure unit model is constructed for vibration reduction.Based on the bandgap vibration reduction mechanism of the acoustic metamaterial periodic structure,the material parameters,structural parameters,and the arrangement interval of the periodic structure unit are optimized.The finite element method(FEM)is used to predict the vibration transmission characteristics of the fluid-conveying pipe installed with the vibration reduction periodic structure.Then,the wave/spectrum element method(WSEM)and experimental test are used to verify the calculated results above.Lastly,the vibration attenuation characteristics of the structure under different conditions,such as rubber material parameters,mass ring material,and fluid-structure coupling effect,are analyzed.The results show that the structure can produce a complete bandgap of 46 Hz-75 Hz in the low-frequency band below 100 Hz,which can effectively suppress the low broadband vibration of the fluidconveying pipe.In addition,a high damping rubber material is used in the design of the periodic structure unit,which realizes the effective suppression of each formant peak of the pipe,and improves the vibration reduction effect of the fluid-conveying pipe.Meanwhile,the structure has the effect of suppressing both bending vibration and longitudinal vibration,and effectively inhibits the transmission of transverse waves and longitudinal waves in the pipe.The research results provide a reference for the application of acoustic metamaterials in the multi-directional vibration control of fluid-conveying pipes.
基金This work was supported by the National Natural Science Foundation of China(Project No.32270278).
文摘Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza,and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo.SmMYB36 has been found to have important regulatory functions in the synthesis of tanshinone and phenolic acid;paradoxically,its mechanism of action in S.miltiorrhiza is not clear.Here,we demonstrated that SmMYB36 functions as a promoter of tanshinones accumulation and a suppressor of phenolic acids through the generation of SmMYB36 overexpressed and chimeric SmMYB36-SRDX(EAR repressive domain)repressor hairy roots in combination with transcriptomic-metabolomic analysis.SmMYB36 directly down-regulate the key enzyme gene of primary metabolism,SmGAPC,up-regulate the tanshinones biosynthesis branch genes SmDXS2,SmGGPPS1,SmCPS1 and down-regulate the phenolic acids biosynthesis branch enzyme gene,SmRAS.Meanwhile,SmERF6,a positive regulator of tanshinone synthesis activating SmCPS1,was up-regulated and SmERF115,a positive regulator of phenolic acid biosynthesis activating SmRAS,was down-regulated.Furthermore,the seven acidic amino acids at the C-terminus of SmMYB36 are required for both self-activating domain and activation of target gene expression.As a consequence,this study contributes to reveal the potential relevance of transcription factors synergistically regulating the biosynthesis of tanshinone and phenolic acid.
基金supported by the National Key Research and Development Program of ChinaNos.2021YFC2 701800 and 2021YFC2 701805 (to QY)+2 种基金Open Research Fund of State Key Laboratory of Genetic EngineeringFudan UniversityNo.SKLGE-21 19 (to TXH and QY)
文摘In Alzheimer’s disease,the transporter P-glycoprotein is responsible for the clearance of amyloid-βin the brain.Amyloid-βcorrelates with the sphingomyelin metabolism,and sphingomyelin participates in the regulation of P-glycoprotein.The amyloid cascade hypothesis describes amyloid-βas the central cause of Alzheimer’s disease neuropathology.Better understanding of the change of P-glycoprotein and sphingomyelin along with amyloid-βand their potential association in the pathological process of Alzheimer’s disease is critical.Herein,we found that the expression of P-glycoprotein in APP/PS1 mice tended to increase with age and was significantly higher at 9 and 12 months of age than that in wild-type mice at comparable age.The functionality of P-glycoprotein of APP/PS1 mice did not change with age but was significantly lower than that of wild-type mice at 12 months of age.Decreased sphingomyelin levels,increased ceramide levels,and the increased expression and activity of neutral sphingomyelinase 1 were observed in APP/PS1 mice at 9 and 12 months of age compared with the levels in wild-type mice.Similar results were observed in the Alzheimer’s disease mouse model induced by intracerebroventricular injection of amyloid-β1-42 and human cerebral microvascular endothelial cells treated with amyloid-β1-42.In human cerebral microvascular endothelial cells,neutral sphingomyelinase 1 inhibitor interfered with the changes of sphingomyelin metabolism and P-glycoprotein expression and functionality caused by amyloid-β1-42 treatment.Neutral sphingomyelinase 1 regulated the expression and functionality of P-glycoprotein and the levels of sphingomyelin and ceramide.Together,these findings indicate that neutral sphingomyelinase 1 regulates the expression and function of P-glycoprotein via the sphingomyelin/ceramide pathway.These studies may serve as new pursuits for the development of anti-Alzheimer’s disease drugs.
基金supported by a grant from the National Natural Science Foundation of China(Grant No.52109163 and 51979188).
文摘Social infrastructures such as dams are likely to be exposed to high risk of terrorist and military attacks,leading to increasing attentions on their vulnerability and catastrophic consequences under such events.This paper tries to develop advanced deep learning approaches for structural dynamic response prediction and dam health diagnosis.At first,the improved long short-term memory(LSTM)networks are proposed for data-driven structural dynamic response analysis with the data generated by a single degree of freedom(SDOF)and the finite numerical simulation,due to the unavailability of abundant practical structural response data of concrete gravity dam under blast events.Three kinds of LSTM-based models are discussed with the various cases of noise-contaminated signals,and the results prove that LSTM-based models have the potential for quick structural response estimation under blast loads.Furthermore,the damage indicators(i.e.,peak vibration velocity and domain frequency)are extracted from the predicted velocity histories,and their relationship with the dam damage status from the numerical simulation is established.This study provides a deep-learning based structural health monitoring(SHM)framework for quick assessment of dam experienced underwater explosions through blastinduced monitoring data.
基金supported by the Natural Science Foundation of Jiangsu Province(Grant No.BK20180855)Research Fund of State Key Laboratory of Mechanics and Control of Mechanical Structures(Grant No.MCMS-E-0219Y01)Research and Practice Innovation Program of postgraduates in Jiangsu Province(Grant No.KYCX20-3076)。
文摘Potential damage in composite structures caused by hail ice impact is an essential safety threat to the aircraft in flight.In this study,a nonlinear finite element(FE)model is developed to investigate the dynamic response and damage behavior of hybrid corrugated sandwich structures subjected to high velocity hail ice impact.The impact and breaking behavior of hail are described using the FE-smoothed particle hydrodynamics(FE-SPH)method.A rate-dependent progressive damage model is employed to capture the intra-laminar damage response;cohesive element and surface-based cohesive contact are implemented to predict the inter-laminar delamination and sheet/core debonding phenomena respectively.The transient processes of sandwich structure under different hail ice impact conditions are analyzed.Comparative analysis is conducted to address the influences of core shape and impact position on the impact performance of sandwich structures and the corresponding energy absorption characteristics are also revealed.
基金the Shenzhen National Science Fund for Distinguished Young Scholars(No.RCJC20210706091948015)National Science Fund for Distinguished Young Scholars(No.52225403)the National Natural Science Foundation of China(No.51827901).
文摘To provide a more accurate evaluation of the scale of deep underground resources,a new pressure coring system was carefully developed and its strength and safety of the engineering were verified by theoretical analyses and numerical simulation.The designed pressure coring system can obtain cores with length of 3 m and diameter of 50 mm at 70 MPa.The results of the ball-drop operation experiment demonstrate that differential motion assembly can effectively cut a safety pin by applying a tensile force of 4852 N,and it can lift the core tube through the center pole to complete a series of mechanical actions to seal the pressure.Additionally,by maintaining pressures at 70 MPa for 30 min,the pressure sealing capacity of the system was proven.Furthermore,a core sample with a diameter of 50 mm was obtained through a core drilling experiment and the coring performance of the pressure coring system was verified.This study can not only enrich the existing onshore coring technology but also provide a theoretical guide and design criteria for the development of similar pressure coring systems to meet the demand for deeper underground exploration.
基金Supported by Hangzhou Medical Health Science and Technology Project,No.20220919Y001and No.20220919Y004.
文摘BACKGROUND Through significant advances in the treatment of peripheral arterial occlusive disease,acute ischemia of the lower extremity is still associated with significant morbidity,limb threat and mortality.The two main causes of acute ischemia in lower extremities are arterial embolism and atherosclerotic arteries.Timely recognition and treatment of acute limb ischemia in emergency situations is essential in order to minimize the duration of ischemia.AIM To investigate the application effect of angiojet thrombolysis in the treatment of acute lower extremity arterial embolization.METHODS Sixty-two patients with acute lower extremity arterial embolization admitted to our hospital from May 2018 to May 2020 were selected.Among them,the observation group(twenty-eight cases)had received angiojet thrombolysis,and the control group(thirty-four cases)had received femoral artery incision and thrombectomy.After thrombus clearance,significant residual stenosis of the lumen was combined with balloon dilation and/or stent implantation.When the thrombus removal was not satisfactory,catheter-directed thrombolysis was performed.The incidence of postoperative complications,recurrence rate and recovery of the two groups were compared.RESULTS There were no significant differences in postoperative recurrence(target vessel reconstruction rate),anklebrachial index and the incidence of postoperative complications between the two groups(P>0.05);there were statistically significant differences in postoperative pain score and postoperative rehabilitation between the two groups(P<0.05).CONCLUSION The application of angiojet in the treatment of acute lower limb artery thromboembolism disease is safe and effective,minimally invasive,quicker recovery after operation,less postoperative complications,which is more suitable for the treatment of femoral popliteal arterial thromboembolism lesions.If the thrombus removal is not satisfactory,the combination of coronary artery aspiration catheter and catheterized directed thrombolysis can be used.Balloon dilation and stent implantation can be considered for obvious lumen stenosis.
基金supported by the Natural Science Foundation of Inner Mongolia Autonomous Region of China,No.2017LH0301(to JXJ),2016MS08108(to ZJY)Science and Technology Planning Project of Inner Mongolia Autonomous Region of China,No.201602069(to ZJY)+1 种基金PhD Scientific Research Fund of Baotou Medical College of China,No.BSJJ201606(to JXJ)"Dengfeng Project" Scientific Research Fund of Baotou Medical College of China,No.BYJJ-DF 201703(to JXJ)
文摘Cognitive dysfunction in Alzheimer's disease is strongly associated with a reduction in synaptic plasticity, which may be induced by oxidative stress. Testosterone is beneficial in learning and memory, although the underlying protective mechanism of testosterone on cognitive performance remains unclear. This study explored the protective mechanism of a subcutaneous injection of 0.75 mg testosterone on cognitive dysfunction induced by bilateral injections of amyloid beta 1–42 oligomers into the lateral ventricles of male rats. Morris water maze test results demonstrated that testosterone treatment remarkably reduced escape latency and path length in Alzheimer's disease rat models. During probe trials, testosterone administration significantly elevated the percentage of time spent in the target quadrant and the number of platform crossings. However, flutamide, an androgen receptor antagonist, inhibited the protective effect of testosterone on cognitive performance in Alzheimer's disease rat models. Nissl staining, immunohistochemistry, western blot assay, and enzyme-linked immunosorbent assay results showed that the number of intact hippocampal pyramidal cells, the dendritic spine density in the hippocampal CA1 region, the immune response and expression level of postsynaptic density protein 95 in the hippocampus, and the activities of superoxide dismutase and glutathione peroxidase were increased with testosterone treatment. In contrast, testosterone treatment reduced malondialdehyde levels. Flutamide inhibited the effects of testosterone on all of these indicators. Our data showed that the protective effect of testosterone on cognitive dysfunction in Alzheimer's disease is mediated via androgen receptors to scavenge free radicals, thereby enhancing synaptic plasticity.
基金supported by the National Natural Science Foundation of China,No.81730033(to XPG),No.81701371(to TJX)
文摘Isoflurane and sevoflurane are both inhalation anesthetics,but in clinical application,sevoflurane has been considered to be less suitable for long-term anesthesia because of its catabolic compounds and potential nephrotoxicity.Nevertheless,recent studies have shown that these two inhalation anesthetics are similar in hepatorenal toxicity,cost,and long-term anesthetic effect.Moreover,sevoflurane possibly has less cognitive impact on young mice.In this study,C57BL/6 mice aged 8–10 weeks were exposed to 1.2%isoflurane or 2.4%sevoflurane for 6 hours.Cognitive function and memory were examined in young mice using the novel object recognition,contextual fear conditioning,and cued-fear extinction tests.Western blot assay was performed to detect expression levels of D1 dopamine receptor,catechol-O-methyltransferase,phospho-glycogen synthase kinase-3β,and total glycogen synthase kinase-3βin the hippocampus.Our results show that impaired performance was not detected in mice exposed to sevoflurane during the novel object recognition test.Contextual memory impairment in the fear conditioning test was shorter in the sevoflurane group than the isoflurane group.Long-term sevoflurane exposure did not affect memory consolidation,while isoflurane led to memory consolidation and reduced retention.Downregulation of hippocampal D1 dopamine receptors and phosphorylated glycogen synthase kinase-3β/total glycogen synthase kinase-3βand upregulation of catechol-O-methyltransferase may be associated with differing memory performance after exposure to isoflurane or sevoflurane.These results confirm that sevoflurane has less effect on cognitive impairment than isoflurane,which may be related to expression of D1 dopamine receptors and catechol-O-methyltransferase and phosphorylation of glycogen synthase kinase-3βin the hippocampus.This study was approved by the Institutional Animal Care and Use Committee,Nanjing University,China on November 20,2017(approval No.20171102).
基金supported by the National Natural Science Foundation of China,No.81730033(to XPG),No.81701371(to TJX),No.81801380(to XZ)the Natural Science Foundation of Jiangsu Province of China,No.BK20170654(to TJX),No.BK20170129(to XZ)the Key Talent’s 13th Five-Year Plan for Strengthening Health of Jiangsu Province of China,No.ZDRCA2016069(to XPG)
文摘Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive dysfunction.Photoacoustic treatments including light and sound stimulation have been found to be effective in modulating sleep patterns and improving cognitive behavior in abnormal sleep-wake pattern experiments.In this study,we examined whether light and sound interventions could reduce sleep-wake pattern disturbances and memory deficits in a sleep rhythm disturbance model.We established a model of sleep rhythm disturbance in C57 BL/6 J mice via a sleep deprivation method involving manual cage tapping,cage jostling,and nest disturbance.We used a Mini Mitter radio transmitter device to monitor motor activity in the mice and fear conditioning tests to assess cognitive function.Our results indicated that an intervention in which the mice were exposed to blue light(40-Hz flickering frequency)for 1 hour during their subjective daytime significantly improved the 24-hour-acrophase shift and reduced the degree of memory deficit induced by sleep deprivation.However,interventions in which the mice were exposed to a 40-Hz blue light at offset time or subjective night time points,as well as 2 Hz-blue light at 3 intervention time points(subjective day time,subjective night time,and offset time points),had no positive effects on circadian rhythm shift or memory deficits.Additionally,a 2000-Hz sound intervention during subjective day time attenuated the24-hour-acrophase shift and memory decline,while 440-Hz and 4000-Hz sounds had no effect on circadian rhythms.Overall,these results demonstrate that photoacoustic treatment effectively corrected abnormal sleep-wake patterns and cognitive dysfunction associated with sleep-deprivation-induced disturbances in sleep-wake rhythm.All animal experiments were approved by the Experimental Animal Ethics Committee of Drum Tower Hospital Affiliated to the Medical College of Nanjing University,China(approval No.20171102)on November20,2017.