Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton

BACKGROUND Glioma is one of the most common intracranial tumors,characterized by invasive growth and poor prognosis.Actin cytoskeletal rearrangement is an essential event of tumor cell migration.The actin dynamics-related protein scinderin(SCIN)has been reported to be closely related to tumor cell migration and invasion in several cancers.AIM To investigate the role and mechanism of SCIN in glioma.METHODS The expression and clinical significance of SCIN in glioma were analyzed based on public databases.SCIN expression was examined using real-time quantitative polymerase chain reaction and Western blotting.Gene silencing was performed using short hairpin RNA transfection.Cell viability,migration,and invasion were assessed using cell counting kit 8 assay,wound healing,and Matrigel invasion assays,respectively.F-actin cytoskeleton organization was assessed using F-actin staining.RESULTS SCIN expression was significantly elevated in glioma,and high levels of SCIN were associated with advanced tumor grade and wild-type isocitrate dehydrogenase.Furthermore,SCIN-deficient cells exhibited decreased proliferation,migration,and invasion in U87 and U251 cells.Moreover,knockdown of SCIN inhibited the RhoA/focal adhesion kinase(FAK)signaling to promote F-actin depolymerization in U87 and U251 cells.CONCLUSION SCIN modulates the actin cytoskeleton via activating RhoA/FAK signaling,thereby promoting the migration and invasion of glioma cells.This study identified the cancer-promoting effect of SCIN and provided a potential therapeutic target for the treatment of glioma.

Toward understanding the microstructure characteristics,phase selection and magnetic properties of laser additive manufactured Nd-Fe-B permanent magnets

Nd-Fe-B permanent magnets play a crucial role in energy conversion and electronic devices.The essential magnetic properties of Nd-Fe-B magnets,particularly coercivity and remanent magnetization,are significantly infuenced by the phase characteristics and microstructure.In this work,Nd-Fe-B magnets were manufactured using vacuum induction melting(VIM),laser directed energy deposition(LDED)and laser powder bed fusion(LPBF)technologies.Themicrostructure evolution and phase selection of Nd-Fe-B magnets were then clarified in detail.The results indicated that the solidification velocity(V)and cooling rate(R)are key factors in the phase selection.In terms of the VIM-casting Nd-Fe-B magnet,a large volume fraction of theα-Fe soft magnetic phase(39.7 vol.%)and Nd2Fe17Bxmetastable phase(34.7 vol.%)areformed due to the low R(2.3×10-1?C s-1),whereas only a minor fraction of the Nd2Fe14B hard magnetic phase(5.15 vol.%)is presented.For the LDED-processed Nd-Fe-B deposit,although the Nd2Fe14B hard magnetic phase also had a low value(3.4 vol.%)as the values of V(<10-2m s-1)and R(5.06×103?C s-1)increased,part of theα-Fe soft magnetic phase(31.7vol.%)is suppressed,and a higher volume of Nd2Fe17Bxmetastable phases(47.5 vol.%)areformed.As a result,both the VIM-casting and LDED-processed Nd-Fe-B deposits exhibited poor magnetic properties.In contrast,employing the high values of V(>10-2m s-1)and R(1.45×106?C s-1)in the LPBF process resulted in the substantial formation of the Nd2Fe14B hard magnetic phase(55.8 vol.%)directly from the liquid,while theα-Fe soft magnetic phase and Nd2Fe17Bxmetastable phase precipitation are suppressed in the LPBF-processed Nd-Fe-B magnet.Additionally,crystallographic texture analysis reveals that the LPBF-processedNd-Fe-B magnets exhibit isotropic magnetic characteristics.Consequently,the LPBF-processed Nd-Fe-B deposit,exhibiting a coercivity of 656 k A m-1,remanence of 0.79 T and maximum energy product of 71.5 k J m-3,achieved an acceptable magnetic performance,comparable to other additive manufacturing processed Nd-Fe-B magnets from MQP(Nd-lean)Nd-Fe-Bpowder.

Demonstration of a small‐scale power generator using supercritical CO_(2)

The supercritical CO_(2)(sCO_(2))power cycle could improve efficiencies for a wide range of thermal power plants.The sCO_(2)turbine generator plays an important role in the sCO_(2)power cycle by directly converting thermal energy into mechanical work and electric power.The operation of the generator encounters challenges,including high temperature,high pressure,high rotational speed,and other engineering problems,such as leakage.Experimental studies of sCO_(2)turbines are insufficient because of the significant difficulties in turbine manufacturing and system construction.Unlike most experimental investigations that primarily focus on 100 kW‐or MW‐scale power generation systems,we consider,for the first time,a small‐scale power generator using sCO_(2).A partial admission axial turbine was designed and manufactured with a rated rotational speed of 40,000 rpm,and a CO_(2)transcritical power cycle test loop was constructed to validate the performance of our manufactured generator.A resistant gas was proposed in the constructed turbine expander to solve the leakage issue.Both dynamic and steady performances were investigated.The results indicated that a peak electric power of 11.55 kW was achieved at 29,369 rpm.The maximum total efficiency of the turbo‐generator was 58.98%,which was affected by both the turbine rotational speed and pressure ratio,according to the proposed performance map.

煤炭地下气化通道传热下顶板岩温度和应力研究

针对煤炭地下气化中的顶板岩稳定和气化热利用问题,建立了热—流—固耦合的流场和应力场计算模型,模拟研究了气化通道传热下顶板岩的温度、应力变化。结果表明,燃烧面前的顶板岩升温幅度低于燃烧面后的;随着燃烧面的推进,气化通道周围顶板岩的升温幅度和升温区域增大;高温可大幅增加顶板岩底部的拉应力和剪应力,最大可增加10倍以上;最大拉应力和最大剪应力发生在燃烧面所在的顶板岩底部位置,顶板岩主要发生拉伸破坏。

不同温度下绿砂岩微观结构及热损伤规律试验研究

为探讨不同温度作用下绿砂岩微细观结构变化及热损伤规律,采用扫描电子显微镜等试验手段,对热处理后的绿砂岩表观形态及微观结构变化进行研究。结果表明:温度对绿砂岩的微细观结构具有重要影响。试件表观形态变化的临界温度为400℃;当温度高于600℃时,砂岩内部的微裂纹数量明显增加,裂隙网络的贯通性增强;1000℃时微裂纹增加最为显著。绿砂岩微观结构变化的主要原因是内部矿物颗粒的性质不同,导致不同温度下产生不同的微观变化。

Effect of hot isostatic pressure on the microstructure and tensile properties of γ'-strengthened superalloy fabricated through induction-assisted directed energy deposition

The microstructure characteristics and strengthening mechanism of Inconel738LC(IN-738LC) alloy prepared by using induction-assisted directed energy deposition(IDED) were elucidated through the investigation of samples subjected to IDED under 1050℃ preheating with and without hot isostatic pressing(HIP,1190℃,105 MPa,and 3 h).Results show that the as-deposited sample mainly consisted of epitaxial columnar crystals and inhomogeneously distributed γ’ phases in interdendritic and dendritic core regions.After HIP,grain morphology changed negligibly,whereas the size of the γ’ phase became increasingly even.After further heat treatment(HT,1070℃,2 h + 845℃,24 h),the γ’ phase in the as-deposited and HIPed samples presented a bimodal size distribution,whereas that in the as-deposited sample showed a size that remained uneven.The comparison of tensile properties revealed that the tensile strength and uniform elongation of the HIP + HTed sample increased by 5% and 46%,respectively,due to the synergistic deformation of bimodal γ’phases,especially large cubic γ’ phases.Finally,the relationship between phase transformations and plastic deformations in the IDEDed sample was discussed on the basis of generalized stability theory in terms of the trade-off between thermodynamics and kinetics.

Ethanol extract of Abelmoschus manihot suppresses endoplasmic reticulum stress in contrast-induced nephropathy

Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2)with iopromide-induced injury were employed to mimic CIN conditions.The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated.In rat models,kidney function,histology,oxidative stress and apoptosis were determined.In HK-2 cells,cell viability,apoptosis,mitochondrial membrane potential,and endoplasmic reticulum stress were assessed.Results:Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats.Additionally,the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation.Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.

催化剂BCE-H 200在高密度聚乙烯装置上的应用

在中韩(武汉)石油化工有限公司Hostalen ACP高密度聚乙烯装置上,采用国产催化剂BCE-H 200分别替代进口催化剂Z 501和Z 509生产了高密度聚乙烯注塑料和薄膜料。分析对比了3种催化剂的活性及粉料粒径分布、堆积密度,并对生产的注塑料和薄膜料进行了表征分析。结果表明:BCE-H 200的活性较Z 501低13.8%,较Z 509高49.2%,其氢调敏感性较Z 501和Z 509低;BCE-H 200生产注塑料和薄膜料时,粉料的粒径分布比进口催化剂Z 501和Z 509更集中,细粉更少,但堆积密度低;采用催化剂BCE-H 200替代催化剂Z 501生产的注塑料刚性较高,韧性较低,相对分子质量分布更宽;采用催化剂BCE-H 200替代催化剂Z 509生产的薄膜料,力学性能变化不大,相对分子质量分布更宽。

粤味童行:岭南美食玩具书设计

基于岭南美食的玩具书设计,是一种结合地方特色美食文化与儿童互动学习的创新方式。这样的书籍不仅提供视觉上的美感,还能激发儿童的味觉和触觉感知,通过多感官的交互增强学习体验。

汽车尺寸精致工艺的开发及提升

随着汽车行业的竞争加剧,围绕质量提升、成本降低、交付加速的新车型开发正在进行优化。为了更好地提升视觉感知质量,文章对汽车尺寸精致工艺进行了研究,建立了相关流程并明确了子车型各阶段的关注点,重点对设计阶段的精致工艺评价方法进行了说明。方法包括:规范评价视角和评价维度,利用CATIA和VRED等软件进行数模分析和渲染分析,并且使用穿戴设备进行了虚拟现实模拟;归纳分缝的方案,从“视觉间隙、视觉面差、外观R角”这3个维度展开说明精致感提升的具体方法。

煤炭地下气化典型有机物热解产出研究

煤炭地下气化过程中尤其是干馏干燥区,热解产生的大量有机物,会对周围含水层造成潜在的污染风险,严重制约煤炭地下气化的产业化发展。通过对煤样进行200~600℃不同终温下慢速热解,模拟气化通道干馏干燥区无氧条件下的热解反应实验,探究煤热解产生典型有机质种类及含量的变化规律。研究表明,热解温度为200℃时,热解产物中含氧化合物含量最高,高达36.19%,随着温度升高,其含量变化较小;酚类化合物随着热解温度的升高而增多,600℃时含量达到17.97%;芳香烃化合物在原煤中含量较高,温度高于300℃时开始大量释放,占热解总产物的比重较高;在300℃时热解产生了大量脂肪烃化合物,随着温度继续升高其含量不断下降,600℃时脂肪烃含量下降至总产物的10%左右。

Direct fitting measurement of gas content in coalbed and selection of reasonable sampling time

In this study, we selected 9 typical coal samples with different metamorphic grades as the study subjects,measured their initial 30-min gas desorption at 30℃ and different pressure using a self-developed gas adsorption/desorption device. Based on the characteristics of gas desorption from coal samples, we proposed a direct fitting method for measurement of gas content in coalbed, analyzed the effects of sampling time on the measurement results and determined the reasonable sampling time of coal samples with different metamorphic grades at different gas adsorption pressure at equilibrium. The results show that (1)the error of gas contents obtained using the direct fitting method relative to that obtained using indirect method is less than 10%, which meets the actual on-site requirements and verifies the feasibility of the direct fitting method;(2) when the relative error is controlled within ±10%, the reasonable sampling time of coal samples is linearly related to the gas adsorption pressure at equilibrium;(3) the reasonable sampling time of coal samples with the same metamorphic grade exhibits a shortening trend with increasing gas adsorption pressure at equilibrium;(4) for coal samples with similar gas adsorption pressure at equilibrium, the reasonable sampling time of coal samples displays a shortening trend with increasing metamorphic grade. Overall, the study provides a basis for improving the measurement accuracy of gas content in coalbed.

煤炭地下气化燃空区残留物中重金属在模拟围岩中的迁移规律研究

煤炭地下气化燃空区中重金属残留物在地下水的浸出作用下会溶解于地下水中,并在高温产生的围岩裂隙中进行迁移扩散,对周围的地下水造成潜在风险。本文研究了同一渗透压条件下,受不同热损伤的模拟煤层围岩多孔介质中重金属离子的种类及迁移富集情况,得到了以下主要结论:重金属离子渗透迁移的距离与多孔介质热损伤有关;受层析原理影响,不同重金属离子在多孔介质中的迁移能力有所差异,锌离子和砷离子的迁移扩散能力最强。

快速水雾化降尘技术在隧道爆破中的应用

爆破作业是地铁隧道施工常用的技术手段,但随着隧道长度、深度的延伸,地铁隧道掘进过程中,常出现爆后粉尘弥漫时间长、降尘效率低等问题,严重威胁施工人员的身体健康,降低施工效率。为更加高效快速地减少钻爆法施工过程中产生的粉尘颗粒,提出了一种快速水雾化除尘技术。依托青岛地铁6号线华山1路西侧斜井工程进行现场试验,利用液态CO_(2)吸热产生的相位变化提升储罐内压,形成高速、高压的水射流可以远距离喷射至掌子面处,最终通过雾化的水滴将粉尘颗粒包裹成团,通过重力自降达到快速降尘的目的。研究结果表明:在爆后15 min范围内,快速水雾化除尘技术可使爆破后产生的PM10、TSP浓度的最大降幅分别达到24.84%、27.68%。快速水雾化降尘技术可以短时间内达到爆后除尘的目的,有效改善施工环境,加快施工进度。

广东省某屠宰场猪源肠球菌的分离鉴定及耐药性和毒力基因分析

【目的】了解猪源性肠球菌分离株的耐药情况及毒力因子携带情况,为肠球菌的耐药监测提供数据支持并为制定合理的治疗方案提供科学依据。【方法】本研究对广州市某大型屠宰场屠宰环节156份猪小肠样品进行肠球菌的分离培养、革兰染色、PCR鉴定,采用琼脂扩散法对菌株进行14种抗菌药物和2种消毒剂的最小抑菌浓度(MIC)测定,利用PCR技术检测耐药基因、毒力基因和消毒剂抗性基因的携带情况。【结果】本研究分离到84株肠球菌(分离率为53.85%),包括44株粪肠球菌和40株屎肠球菌;分离培养可见菌落边缘光滑整齐、圆形或卵圆形排列、菌落周围培养基呈现黑色的菌落;疑似菌落革兰染色后呈圆形的革兰阳性球菌,单个或成堆排列在一起。对疑似菌落DNA进行PCR扩增,其中粪肠球菌引物扩增出大小约941 bp的条带,16S rRNA引物扩增出大小约1500 bp的条带。药敏试验结果显示,44株粪肠球菌对克林霉素、多西环素、头孢噻呋、庆大霉素、红霉素、苯扎氯铵和氟苯尼考的耐药率较高,分别为97.7%、90.9%、88.6%、88.6%、81.8%、81.8%和77.3%;40株屎肠球菌对克林霉素、头孢噻呋、多西环素、庆大霉素、氟苯尼考和红霉素的耐药率分别为90.0%、90.0%、85.0%、77.5%、72.5%和67.5%。毒力基因与消毒剂抗性基因检测结果显示,粪肠球菌efa、gelE、fsrC毒力基因的检出率较高,分别为95.5%、84.1%和84.1%;emeA、gsp 65消毒剂抗性基因的检出率较高,分别为95.5%和90.9%。屎肠球菌仅检测到fsrB毒力基因,检出率为2.5%,无消毒剂抗性基因的检出。【结论】本研究分离的猪源肠球菌对大部分抗菌药物具有较高的耐药率,部分菌株还对利奈唑胺产生耐药,亟需加强对猪源肠球菌的流行病学监测。

煤升温过程中声发射信号变化及产生机制研究

煤自燃预警存在火灾信息感知难的问题,基于煤岩热损伤破坏产生的声发射信号能够进行温度感知,进而预警煤自燃演化的状态。为研究煤在升温及燃烧过程中声发射信号变化,探究煤自燃过程中不同温度阶段煤释放的声发射信号差异及时域、频域变化,并研究声发射产生机制,可为声发射信号反演煤体温度、监测煤自燃提供理论基础。构建了声发射信号测试系统,选取东滩煤矿的烟煤进行试验,测试煤在升温过程中的声发射信号,分析煤在温度作用下变形破裂演化过程以及煤体成分变化,探讨煤升温过程中的声发射信号产生机制。研究表明:煤在升温过程中,伴随产生声发射信号,煤在升温直至燃烧过程中振铃计数呈阵发性增长,通过线性拟合得到相关系数超过0.87,表明煤在升温过程中变形破裂的频次呈增大的趋势;随着温度的升高,煤体产生声发射信号振铃计数增长率与声发射平均频率(AF值)在初始升温阶段较低,在快速升温时数值较高;累计损伤参数在煤温超过80℃时出现拐点,主要损伤模式由快速扩展损伤变为渐进式损伤。通过分析不同温度煤体结构和成分的宏微观变化可知,随着温度升高,煤中的矿物转化速率影响煤体内部主要损伤模式,高矿物转化速率对应煤体内部快速扩展损伤,促使煤体孔裂隙衍生扩展,产生逐渐增大的声发射信号。

Surgical treatment for recurrent hepatocellular carcinoma:Current status and challenges

Primary liver cancer is the sixth most commonly diagnosed cancer and was the third leading cause of cancer deaths worldwide in 2020.It includes hepatocellular carcinoma(HCC)(representing 75%-85%of cases),intrahepatic cholangiocarcinoma(representing 10%-15%of cases),and other rare types.The survival rate of patients with HCC has risen with improved surgical technology and perioperative management in recent years;however,high tumor recurrence rates continue to limit long-term survival,even after radical surgical resection(exceeding 50%recurrence).For resectable recurrent liver cancer,surgical removal[either salvage liver transplantation(SLT)or repeat hepatic resection]remains the most effective therapy that is potentially curative for recurrent HCC.Thus,here,we introduce surgical treatment for recurrent HCC.Areas Covered:A literature search was performed for recurrent HCC using Medline and PubMed up to August 2022.Expert commentary:In general,long-term survival after the reresection of recurrent liver cancer is usually beneficial.SLT has equivalent outcomes to primary liver transplantation for unresectable recurrent illness in a selected group of patients;however,SLT is constrained by the supply of liver grafts.SLT seems to be inferior to repeat liver resection when considering operative and postoperative results but has the major advantage of disease-free survival.When considering the similar overall survival rate and the current situation of donor shortages,repeat liver resection remains an important option for recurrent HCC.

The “No-touch” technique improves the survival of patients with advanced hepatocellular carcinomas treated by liver transplantation: A single-center prospective randomized controlled trial

Background:Liver transplantation(LT)is the best treatment for patients with hepatocellular carcinoma(HCC).However,the surgical technique needs to be improved.The present study aimed to evaluate the“no-touch”technique in LT.Methods:From January 2018 to December 2019,we performed a prospective randomized controlled trial on HCC patients who underwent LT.The patients were randomized into two groups:a no-touch technique LT group(NT group,n=38)and a conventional LT technique group(CT group,n=46).Operative outcomes and survival in the two groups were analyzed.Results:The perioperative parameters were comparable between the two groups(P>0.05).There was no significant difference between the two groups in disease-free survival(DFS)(P=0.732)or overall survival(OS)(P=0.891).Of 36 patients who were beyond the Hangzhou criteria for LT,the DFS of the patients in the NT group was significantly longer than that in the CT group(median 402 vs.126 days,P=0.025).In 31 patients who had portal vein tumor thrombosis(PVTT),DFS and OS in the NT group were significantly better than those in the CT group(median DFS 420 vs.167 days,P=0.022;2-year OS rate 93.8%vs.66.7%,P=0.043).In 14 patients who had diffuse-type HCCs,DFS and OS were significantly better in the NT group than those in the CT group(median DFS 141 vs.56 days,P=0.008;2-year OS rate 75.0%vs.33.3%,P=0.034).Multivariate analysis showed that for patients with PVTT and diffusetype HCCs,the no-touch technique was an independent favorable factor for OS(PVTT:HR=0.018,95%CI:0.001-0.408,P=0.012;diffuse-type HCCs:HR=0.034,95%CI:0.002-0.634,P=0.024).Conclusions:The no-touch technique improved the survival of patients with advanced HCC compared with the conventional technique.The no-touch technique may provide a new and effective LT technique for advanced HCCs.

Blob properties in I-mode and ELM-free H-mode compared to L-mode on EAST

The blob properties in I-mode and ELM-free H-mode plasmas compared to L-mode have been investigated on the EAST tokamak,including the blob detection rate Nb,sizeδb,lifetimeτb and radial velocity vr,b.The blob properties in L-mode and I-mode are similar,and those in ELM-free H-mode are different to them.The blob Nbis smaller whileτbis larger in H-mode.The experimental blob sizeδband velocity scalings vr,b-δb show a good agreement with the theoretical models.The variation in blob properties during the L-I and H-L transitions,and their relations to the scrape-off layer(SOL)density,edge and SOL turbulence,and SOL collisionality are discussed.The suppression of the edge(inside the last closed flux surface)turbulence is not reflected in the blob behavior,while the blob detection rate shows a correlation with the SOL density and its low-frequency(3–50 kH z)fluctuations.In addition,the blob detection rate is found to increase with the divertor collisionalityΛdiv,indicating a dependence of blob behavior onΛdiv.The differences in blob detection rates among the three operating regimes might be due to their different SOL densities and collisionalities.The investigation contributes to understanding the influences of edge and SOL plasma parameters on the blob behavior.

Diagnosis and treatment of McCune-Albright syndrome:A case report

BACKGROUND McCune-Albright syndrome(MAS)is extremely rare clinically.We here report a case of MAS with severe symptoms that have not been reported previously.CASE SUMMARY A 10-year-old boy attended our outpatient clinic due to craniofacial malformations found two years ago.He underwent temporal bone computed tomography and digital radiography photography.Based on a literature review combined with the patient's medical history and imaging examination findings,he was diagnosed with multiple fibrous dysplasia of bone.As the clinical symptoms related to MAS in this patient were not obvious,he was only followed up and not given any special treatment.CONCLUSION The unique clinical manifestations in this MAS patient may be related to mutations in the GNAS gene.