Non-alcoholic fatty liver disease(NAFLD)is associated with mutations in lipopolysaccharide-binding protein(LBP),but the underlying epigenetic mechanisms remain understudied.Herein,LBP^(-/-)rats with NAFLD were establi...Non-alcoholic fatty liver disease(NAFLD)is associated with mutations in lipopolysaccharide-binding protein(LBP),but the underlying epigenetic mechanisms remain understudied.Herein,LBP^(-/-)rats with NAFLD were established and used to conduct integrative targetingactive enhancer histone H3 lysine 27 acetylation(H3K27ac)chromatin immunoprecipitation coupled with high-throughput and transcriptomic sequencing analysis to explore the potential epigenetic pathomechanisms of active enhancers of NAFLD exacerbation upon LBP deficiency.Notably,LBP^(-/-)reduced the inflammatory response but markedly aggravated high-fat diet(HFD)-induced NAFLD in rats,with pronounced alterations in the histone acetylome and regulatory transcriptome.In total,1128 differential enhancer-target genes significantly enriched in cholesterol and fatty acid metabolism were identified between wild-type(WT)and LBP^(-/-)NAFLD rats.Based on integrative analysis,CCAAT/enhancer-binding proteinβ(C/EBPβ)was identified as a pivotal transcription factor(TF)and contributor to dysregulated histone acetylome H3K27ac,and the lipid metabolism gene SCD was identified as a downstream effector exacerbating NAFLD.This study not only broadens our understanding of the essential role of LBP in the pathogenesis of NAFLD from an epigenetics perspective but also identifies key TF C/EBPβand functional gene SCD as potential regulators and therapeutic targets.展开更多
Insufficient observations near the origin of the Kuroshio have led to incomplete understanding of the intraseasonal variability(ISV)of the Kuroshio.Direct measurements of the Kuroshio velocity were performed with an a...Insufficient observations near the origin of the Kuroshio have led to incomplete understanding of the intraseasonal variability(ISV)of the Kuroshio.Direct measurements of the Kuroshio velocity were performed with an array of three profiler moorings(122.7°E,123°E,and 123.3°E)along 18°N from January 2018 to February 2020.The ISV of the Kuroshio at 18°N was investigated based on a combination of mooring observations and global high-resolution HYbrid Coordinate Ocean Model reanalysis data.The estimated time-averaged transport in the upper 350 m across the observation transect was 6.5±2.6 Sv(1.0 Sv=10^(6)m^(3)/s).Two significant ISV peaks at 50-60 and~100 d were recognized in the power spectra of the meridional velocity and transport.Further analysis indicated that the ISV at 50-60 d was caused by westward-propagating eddies at average propagation speed of~13 cm/s and wavelength of~635 km.Another ISV peak at~100 d was mainly caused by northward-propagating eddies generated in the North Equatorial Current region.Further investigation indicated that the ISV of the Kuroshio at 18°N is dominated by meridional transport,rather than by the zonal migration of the Kuroshio main axis.Our findings provide a better understanding of the ISV of the Kuroshio east of Luzon Island.展开更多
BACKGROUND Stem cell therapy has shown great potential for treating diabetic foot(DF).AIM To conduct a bibliometric analysis of studies on the use of stem cell therapy for DF over the past two decades,with the aim of ...BACKGROUND Stem cell therapy has shown great potential for treating diabetic foot(DF).AIM To conduct a bibliometric analysis of studies on the use of stem cell therapy for DF over the past two decades,with the aim of depicting the current global research landscape,identifying the most influential research hotspots,and providing insights for future research directions.METHODS We searched the Web of Science Core Collection database for all relevant studies on the use of stem cell therapy in DF.Bibliometric analysis was carried out using CiteSpace,VOSviewer,and R(4.3.1)to identify the most notable studies.RESULTS A search was conducted to identify publications related to the use of stem cells for DF treatment.A total of 542 articles published from 2000 to 2023 were identified.The United States had published the most papers on this subject.In this field,Iran’s Shahid Beheshti University Medical Sciences demonstrated the highest productivity.Furthermore,Dr.Bayat from the same university has been an outstanding researcher in this field.Stem Cell Research&Therapy is the journal with the highest number of publications in this field.The main keywords were“diabetic foot ulcers,”“wound healing,”and“angiogenesis.”CONCLUSION This study systematically illustrated the advances in the use of stem cell therapy to treat DF over the past 23 years.Current research findings suggested that the hotspots in this field include stem cell dressings,exosomes,wound healing,and adipose-derived stem cells.Future research should also focus on the clinical translation of stem cell therapies for DF.展开更多
高比例可再生能源的接入给微电网的稳定运行带来的巨大挑战,传统随机优化和鲁棒优化方法在处理不确定性方面仍存在一定的局限性。综合考虑风电、负荷的双重不确定性,采用分布鲁棒优化方法,构建了基于豪斯多夫(Hausdorff)距离的微电网两...高比例可再生能源的接入给微电网的稳定运行带来的巨大挑战,传统随机优化和鲁棒优化方法在处理不确定性方面仍存在一定的局限性。综合考虑风电、负荷的双重不确定性,采用分布鲁棒优化方法,构建了基于豪斯多夫(Hausdorff)距离的微电网两阶段分布鲁棒优化调度模型,第一阶段利用场景预测信息,制定包含储能充放电计划、购售电计划的预调度方案,第二阶段基于各典型场景信息制定微电网各单元出力调整计划;然后采用列与约束生成算法(columns and constraints generation,CCG)进行迭代求解,得到最恶劣概率分布下运行成本最低的调度方案;最后,通过算例仿真分析验证了所提方法的有效性,所得到的决策方案兼顾了经济性和安全性。展开更多
Tbx18,Wt1,and Tcf21 have been identified as epicardial markers during the early embryonic stage.However,the gene markers of mature epicardial cells remain unclear.Single-cell transcriptomic analysis was performed with...Tbx18,Wt1,and Tcf21 have been identified as epicardial markers during the early embryonic stage.However,the gene markers of mature epicardial cells remain unclear.Single-cell transcriptomic analysis was performed with the Seurat,Monocle,and CellphoneDB packages in R software with standard procedures.Spatial transcriptomics was performed on chilled Visium Tissue Optimization Slides(10x Genomics)and Visium Spatial Gene Expression Slides(10x Genomics).Spatial transcriptomics analysis was performed with Space Ranger software and R software.Immunofluorescence,whole-mount RNA in situ hybridization and X-gal staining were performed to validate the analysis results.Spatial transcriptomics analysis revealed distinct transcriptional profiles and functions between epicardial tissue and non-epicardial tissue.Several gene markers specific to postnatal epicardial tissue were identified,including Msln,C3,Efemp1,and Upk3b.Single-cell transcriptomic analysis revealed that cardiac cells from wildtype mouse hearts(from embryonic day 9.5 to postnatal day 9)could be categorized into six major cell types,which included epicardial cells.Throughout epicardial development,Wt1,Tbx18,and Upk3b were consistently expressed,whereas genes including Msln,C3,and Efemp1 exhibited increased expression during the mature stages of development.Pseudotime analysis further revealed two epicardial cell fates during maturation.Moreover,Upk3b,Msln,Efemp1,and C3 positive epicardial cells were enriched in extracellular matrix signaling.Our results suggested Upk3b,Efemp1,Msln,C3,and other genes were mature epicardium markers.Extracellular matrix signaling was found to play a critical role in the mature epicardium,thus suggesting potential therapeutic targets for heart regeneration in future clinical practice.展开更多
Longan originates from southern China and has high nutritional and health value.Recent phytochemistry and pharmacology studies have shown that polysaccharides are a main bioactive component of longan.Longan polysaccha...Longan originates from southern China and has high nutritional and health value.Recent phytochemistry and pharmacology studies have shown that polysaccharides are a main bioactive component of longan.Longan polysaccharides possess antioxidant,anti-aging,anti-tumor,immunomodulatory,and other bioactivities.Hot-water extraction,ethanol precipitation,and ultrasonic extraction are generally used to extract water-soluble longan polysaccharides.However,the relationship between the structure and bioactivity of longan polysaccharides remains unclear,requiring further investigation.The aim of this review is to evaluate the current literature focusing on the extraction,purification,structural characterization,and biological activity of longan polysaccharides.We believe that this review would provide a useful bibliography for further innovation and a basis for using longan polysaccharides in functional food.展开更多
In existing remote sensing image retrieval(RSIR)datasets,the number of images among different classes varies dramatically,which leads to a severe class imbalance problem.Some studies propose to train the model with th...In existing remote sensing image retrieval(RSIR)datasets,the number of images among different classes varies dramatically,which leads to a severe class imbalance problem.Some studies propose to train the model with the ranking‐based metric(e.g.,average precision[AP]),because AP is robust to class imbalance.However,current AP‐based methods overlook an important issue:only optimising samples ranking before each positive sample,which is limited by the definition of AP and is prone to local optimum.To achieve global optimisation of AP,a novel method,namely Optimising Samples after positive ones&AP loss(OSAP‐Loss)is proposed in this study.Specifically,a novel superior ranking function is designed to make the AP loss differentiable while providing a tighter upper bound.Then,a novel loss called Optimising Samples after Positive ones(OSP)loss is proposed to involve all positive and negative samples ranking after each positive one and to provide a more flexible optimisation strategy for each sample.Finally,a graphics processing unit memory‐free mechanism is developed to thoroughly address the non‐decomposability of AP optimisation.Extensive experimental results on RSIR as well as conventional image retrieval datasets show the superiority and competitive performance of OSAP‐Loss compared to the state‐of‐the‐art.展开更多
基金supported by the National Natural Science Foundation of China(81971875,82300661)Natural Science Foundation of Anhui province(2308085QH246)+3 种基金Natural Science Foundation of the Anhui Higher Education Institutions(KJ2021A0205)Basic and Clinical Cooperative Research Program of Anhui Medical University(2019xkjT002,2019xkjT022,2022xkjT013)Talent Training Program,School of Basic Medical Sciences,Anhui Medical University(2022YPJH102)National College Students Innovation and Entrepreneurship Training Program of China(202210366024)。
文摘Non-alcoholic fatty liver disease(NAFLD)is associated with mutations in lipopolysaccharide-binding protein(LBP),but the underlying epigenetic mechanisms remain understudied.Herein,LBP^(-/-)rats with NAFLD were established and used to conduct integrative targetingactive enhancer histone H3 lysine 27 acetylation(H3K27ac)chromatin immunoprecipitation coupled with high-throughput and transcriptomic sequencing analysis to explore the potential epigenetic pathomechanisms of active enhancers of NAFLD exacerbation upon LBP deficiency.Notably,LBP^(-/-)reduced the inflammatory response but markedly aggravated high-fat diet(HFD)-induced NAFLD in rats,with pronounced alterations in the histone acetylome and regulatory transcriptome.In total,1128 differential enhancer-target genes significantly enriched in cholesterol and fatty acid metabolism were identified between wild-type(WT)and LBP^(-/-)NAFLD rats.Based on integrative analysis,CCAAT/enhancer-binding proteinβ(C/EBPβ)was identified as a pivotal transcription factor(TF)and contributor to dysregulated histone acetylome H3K27ac,and the lipid metabolism gene SCD was identified as a downstream effector exacerbating NAFLD.This study not only broadens our understanding of the essential role of LBP in the pathogenesis of NAFLD from an epigenetics perspective but also identifies key TF C/EBPβand functional gene SCD as potential regulators and therapeutic targets.
基金Supported by the National Natural Science Foundation of China(Nos.41976011,42022040)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB42010102)+1 种基金the Shandong Provincial Natural Science Foundation(No.ZR2020JQ18)Shijian HU is a member of the CAS Interdisciplinary Innovation Team(No.JCTD2020-12)。
文摘Insufficient observations near the origin of the Kuroshio have led to incomplete understanding of the intraseasonal variability(ISV)of the Kuroshio.Direct measurements of the Kuroshio velocity were performed with an array of three profiler moorings(122.7°E,123°E,and 123.3°E)along 18°N from January 2018 to February 2020.The ISV of the Kuroshio at 18°N was investigated based on a combination of mooring observations and global high-resolution HYbrid Coordinate Ocean Model reanalysis data.The estimated time-averaged transport in the upper 350 m across the observation transect was 6.5±2.6 Sv(1.0 Sv=10^(6)m^(3)/s).Two significant ISV peaks at 50-60 and~100 d were recognized in the power spectra of the meridional velocity and transport.Further analysis indicated that the ISV at 50-60 d was caused by westward-propagating eddies at average propagation speed of~13 cm/s and wavelength of~635 km.Another ISV peak at~100 d was mainly caused by northward-propagating eddies generated in the North Equatorial Current region.Further investigation indicated that the ISV of the Kuroshio at 18°N is dominated by meridional transport,rather than by the zonal migration of the Kuroshio main axis.Our findings provide a better understanding of the ISV of the Kuroshio east of Luzon Island.
基金Supported by:The study was funded by the National Natural Science Foundation of China(82274528)Construction Task Book for the Three-Year Action Plan for Accelerating the Inheritance and Innovative Development of Traditional Chinese Medicine in Shanghai(2021-2023)(ZY(2021-2023)-0211)+4 种基金Shanghai Municipal Health Commission Scientific Research Programme Mission Statement(202240228)Special Youth Project for Clinical Research of Shanghai Municipal Health Commission(20234Y0162)Clinical Research Talent Training Program of Shanghai University of Traditional Chinese Medicine Affiliated Hospital(2023LCRC06)Four Bright Foundations of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine(SGKJ-202301)Shanghai Pudong New Area Health and Family Planning Commission’s Pudong Famous TCM Cultivation Program project(PWRZM2020-18).
文摘BACKGROUND Stem cell therapy has shown great potential for treating diabetic foot(DF).AIM To conduct a bibliometric analysis of studies on the use of stem cell therapy for DF over the past two decades,with the aim of depicting the current global research landscape,identifying the most influential research hotspots,and providing insights for future research directions.METHODS We searched the Web of Science Core Collection database for all relevant studies on the use of stem cell therapy in DF.Bibliometric analysis was carried out using CiteSpace,VOSviewer,and R(4.3.1)to identify the most notable studies.RESULTS A search was conducted to identify publications related to the use of stem cells for DF treatment.A total of 542 articles published from 2000 to 2023 were identified.The United States had published the most papers on this subject.In this field,Iran’s Shahid Beheshti University Medical Sciences demonstrated the highest productivity.Furthermore,Dr.Bayat from the same university has been an outstanding researcher in this field.Stem Cell Research&Therapy is the journal with the highest number of publications in this field.The main keywords were“diabetic foot ulcers,”“wound healing,”and“angiogenesis.”CONCLUSION This study systematically illustrated the advances in the use of stem cell therapy to treat DF over the past 23 years.Current research findings suggested that the hotspots in this field include stem cell dressings,exosomes,wound healing,and adipose-derived stem cells.Future research should also focus on the clinical translation of stem cell therapies for DF.
文摘高比例可再生能源的接入给微电网的稳定运行带来的巨大挑战,传统随机优化和鲁棒优化方法在处理不确定性方面仍存在一定的局限性。综合考虑风电、负荷的双重不确定性,采用分布鲁棒优化方法,构建了基于豪斯多夫(Hausdorff)距离的微电网两阶段分布鲁棒优化调度模型,第一阶段利用场景预测信息,制定包含储能充放电计划、购售电计划的预调度方案,第二阶段基于各典型场景信息制定微电网各单元出力调整计划;然后采用列与约束生成算法(columns and constraints generation,CCG)进行迭代求解,得到最恶劣概率分布下运行成本最低的调度方案;最后,通过算例仿真分析验证了所提方法的有效性,所得到的决策方案兼顾了经济性和安全性。
基金supported by grants from the National Natural Science Foundation of China(Grant No.:82270281)Chongqing Medical University Program for Youth Innovation in Future Medicine(Grant No.:W0133)+2 种基金Senior Medical Talents Program of Chongqing for Young and Middle-aged,China(Program No.:JianlinDu[2022])Postdoctoral Research Funding of the Second Affiliated Hospital of Chongqing Medical University,China(Grant No.:rsc-postdoctor114)and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University,China(Program No.:kryc-gg-2102).
文摘Tbx18,Wt1,and Tcf21 have been identified as epicardial markers during the early embryonic stage.However,the gene markers of mature epicardial cells remain unclear.Single-cell transcriptomic analysis was performed with the Seurat,Monocle,and CellphoneDB packages in R software with standard procedures.Spatial transcriptomics was performed on chilled Visium Tissue Optimization Slides(10x Genomics)and Visium Spatial Gene Expression Slides(10x Genomics).Spatial transcriptomics analysis was performed with Space Ranger software and R software.Immunofluorescence,whole-mount RNA in situ hybridization and X-gal staining were performed to validate the analysis results.Spatial transcriptomics analysis revealed distinct transcriptional profiles and functions between epicardial tissue and non-epicardial tissue.Several gene markers specific to postnatal epicardial tissue were identified,including Msln,C3,Efemp1,and Upk3b.Single-cell transcriptomic analysis revealed that cardiac cells from wildtype mouse hearts(from embryonic day 9.5 to postnatal day 9)could be categorized into six major cell types,which included epicardial cells.Throughout epicardial development,Wt1,Tbx18,and Upk3b were consistently expressed,whereas genes including Msln,C3,and Efemp1 exhibited increased expression during the mature stages of development.Pseudotime analysis further revealed two epicardial cell fates during maturation.Moreover,Upk3b,Msln,Efemp1,and C3 positive epicardial cells were enriched in extracellular matrix signaling.Our results suggested Upk3b,Efemp1,Msln,C3,and other genes were mature epicardium markers.Extracellular matrix signaling was found to play a critical role in the mature epicardium,thus suggesting potential therapeutic targets for heart regeneration in future clinical practice.
基金the National Natural Science Foundation of China(32201969,82204668)Natural Science Foundation of Henan Province(212300410297)+3 种基金Hebei Natural Science Foundation(H2022423376)Basic Research Plan of Higher Education School Key Scientific Research Project of Henan Province(21A550014)Doctoral Research Foundation of Zhengzhou University of Light Industry(2020BSJJ015)Science and Technology Research Project of Higher Education in Hebei Province(QN2020233).
文摘Longan originates from southern China and has high nutritional and health value.Recent phytochemistry and pharmacology studies have shown that polysaccharides are a main bioactive component of longan.Longan polysaccharides possess antioxidant,anti-aging,anti-tumor,immunomodulatory,and other bioactivities.Hot-water extraction,ethanol precipitation,and ultrasonic extraction are generally used to extract water-soluble longan polysaccharides.However,the relationship between the structure and bioactivity of longan polysaccharides remains unclear,requiring further investigation.The aim of this review is to evaluate the current literature focusing on the extraction,purification,structural characterization,and biological activity of longan polysaccharides.We believe that this review would provide a useful bibliography for further innovation and a basis for using longan polysaccharides in functional food.
基金supported by the National Nature Science Foundation of China(No.U1803262,62176191,62171325)Nature Science Foundation of Hubei Province(2022CFB018)financially supported by fund from Hubei Province Key Laboratory of Intelligent Information Processing and Real‐time Industrial System(Wuhan University of Science and Technology)(ZNXX2022001).
文摘In existing remote sensing image retrieval(RSIR)datasets,the number of images among different classes varies dramatically,which leads to a severe class imbalance problem.Some studies propose to train the model with the ranking‐based metric(e.g.,average precision[AP]),because AP is robust to class imbalance.However,current AP‐based methods overlook an important issue:only optimising samples ranking before each positive sample,which is limited by the definition of AP and is prone to local optimum.To achieve global optimisation of AP,a novel method,namely Optimising Samples after positive ones&AP loss(OSAP‐Loss)is proposed in this study.Specifically,a novel superior ranking function is designed to make the AP loss differentiable while providing a tighter upper bound.Then,a novel loss called Optimising Samples after Positive ones(OSP)loss is proposed to involve all positive and negative samples ranking after each positive one and to provide a more flexible optimisation strategy for each sample.Finally,a graphics processing unit memory‐free mechanism is developed to thoroughly address the non‐decomposability of AP optimisation.Extensive experimental results on RSIR as well as conventional image retrieval datasets show the superiority and competitive performance of OSAP‐Loss compared to the state‐of‐the‐art.
