Objective：Pulmonary fibrosis is a common pathological phenomena in lung cancer patients after chemotherapy or radiotherapy, which is a key factor hindering to transport ion of high concentrated drug to the lung tissu...Objective：Pulmonary fibrosis is a common pathological phenomena in lung cancer patients after chemotherapy or radiotherapy, which is a key factor hindering to transport ion of high concentrated drug to the lung tissue, peptide trans-porter has become targets of the rational design of peptides and peptide drug. The purpose of the study is to investigate the expression of PEPT2 mRNA in the lung of rats with bleomycin （BLM）-induced pulmonary fibrosis. Methods：Fifty healthy adult Spragne-Dawley rats were randomized into five groups, the rats in BLM 7d, 14d and 28d groups were treated with a single instil ation of 5 mg/kg of BLM, to induced pulmonary fibrosis models. On days 7, 14 and 28, the animals were kil ed by exsan-guination respectively. Normal saline （NS） group were treated by NS, on days 14, the animals were kil ed by exsanguinations. Control group were untreated. The lung samples were processed for light microscopy and determined the hydroxyproline （HYP） concentration. The expression of PEPT2 mRNA were measured by RT-PCR. PEPT2 cDNA fragments were tested by dideoxy chain termination. Results：Compared with control and NS group, HYP levels increased on day 7 of BLM group, but there was no statistical significant dif erence （P〉0.05）. HYP levels markedly increased on days 14 and 28 of BLM group, there was statistical significant dif erence （P〈0.01）. The morphological study showed that col agenous fiber proliferated on days 14 and 28 of BLM group, especial y on day 28, formed pulmonary fibrosis. There were no significant changes of pulmo-nary PEPT2 mRNA expression at dif erent groups （P〉0.05）. Conclusion：The pulmonary fibrosis models of SD rats can be induced by a single instil ation of 5 mg/kg of bleomycin on 28d. There were no significant changes of PEPT2 mRNA expression in the lung of rats with bleomycin-induced pulmonary fibrosis.展开更多
Traditional descriptive observation and advanced geometric morphometric are employed to study the morphological characteristics of Zhoukoudian mandibular premolars, and simultaneously with the specimens of Australopit...Traditional descriptive observation and advanced geometric morphometric are employed to study the morphological characteristics of Zhoukoudian mandibular premolars, and simultaneously with the specimens of Australopithecus, African early Homo, Homo erectus in other areas of Asia except Zhoukoudian, Europe Pleistocene fossil hominins, and recent Chinese (72 P3 and 69 P4 on the whole) being included as comparisons. Results suggest obvious evolutionary changes in the occlusal morphology and crown outline shape of mandibular premolars. Australopithecus P3s are found with extremely asymmetrical crown outlines, prominent mesial and distal vertical grooves on the buccal side, open foveas, and well-developed talonids, and P4s with similarly asymmetrical crown outlines, distinct mesial and distal vertical grooves on the buccal side, and "H" type of grooves combination. These characteristics are also expressed in the later hominin specimens, but with a decreased extent and lower frequency. The P3s of recent Chinese, slightly asymmetrical in the crown lingual contours, and P4s, basically symmetrical in the crown outlines, become faint or disappeared in the mesial or distal vertical buccal grooves, and decreased in the talonid size. It is suggested that Zhoukoudian mandibular premolars preserve some primitive traits of earlier hominins, including asymmetrical crown outlines and prominent mesial and distal vertical grooves of buccal side. Moreover, Zhoukoudian specimens exhibit obvious morphological variation between individuals, expressed mainly by aspects of mesial and distal vertical buccal grooves in various degrees, symmetry or asymmetry extent of crown lingual contour, relative location of polygon to the corresponding surrounding outline, as well as the relative size of talonid. When compared with Homo erectus from the other areas of Asia, Zhoukoudian specimens show their similarity with those of Xichuan and S-1, and at the same time great discrepancy with S-6 in the occlusal morphology and crown outline shape. When it comes to the relationship between specimens of Europe Pleistocene and Zhoukoudian, disparity is expressed in a stronger way than the corresponding similarity.展开更多
Background:Disease activity indices(DAIs)including disease activity score 28(DAS28),simplified disease activity index(SDAI),and clinical disease activity index(CDAI)have been widely used in clinical practice and resea...Background:Disease activity indices(DAIs)including disease activity score 28(DAS28),simplified disease activity index(SDAI),and clinical disease activity index(CDAI)have been widely used in clinical practice and research studies of rheumatoid arthritis(RA).The objective of our study was to evaluate the correlation and concordance among different DAIs in Chinese patients with RA.Methods:A cross-sectional study,including patients enrolled in the Chinese registry of rheumatoid arthritis from November 2016 to August 2018,was conducted.The correlations were evaluated using Spearman correlation coefficient and concordance with Bland-Altman plots,quadratic weighted kappa,and discordance rates in the crosstab.For other indices,the optimal cutoff points corresponding to SDAI remission were explored through receiver operating characteristic curve analysis.Results:A total of 30,501 patients were included,of whom 80.46%were women.Most individuals were with moderate disease activity or high disease activity.High correlations among DAS28-erythrocyte sedimentation rate(ESR)and DAS28-C-reactive protein(CRP),SDAI and CDAI were observed.Similarly,the weighted kappa value among the indices was high.In Bland-Altman plots,a positive difference between DAS28-ESR and DAS28-CRP was observed,with an absolute difference of>1.2 in 3079(10.09%)patients.In crosstab,approximately 30%of the patients were classified into different groups.Concordance values between SDAI remission and the optimal cutoff points of DAS28-ESR,DAS28-CRP,and CDAI were 3.06,2.37,and 3.20,respectively.Conclusions:Although DAIs had high correlations and weighted kappa values,the discordance between DAIs was significant in Chinese patients with RA.The four DAIs are not interchangeable.展开更多
Pt-based ultrathin nanowires (NWs) are considered as one of the most intriguing catalysts for fuel cells.However,the delicate controllability of surface structure of ultrathin NWs to regulate their catalytic performan...Pt-based ultrathin nanowires (NWs) are considered as one of the most intriguing catalysts for fuel cells.However,the delicate controllability of surface structure of ultrathin NWs to regulate their catalytic performances is still a challenge.Here,two kinds of one-nanometer-thick Pt-based NWs with smooth surfaces (S-NWs) and rough surfaces (R-NWs) are demonstrated,in which the combined use of hexadecyltrimethylammonium bromide and oleylamine plays an essential role,as they could form soft-templates to direct the growth of NWs.Due to its high-density of low-coordinated sites on the surface,Pt-based R-NWs exhibit higher oxygen reduction reaction (ORR) activities but lower stabilities than corresponding S-NWs.Notably,Pt0.78Ni0.22 R-NWs possess the highest mass activity (1.07 A-mgpt^-1) and specific activity (1.02 mA·cm^-2) among all Pt-based NWs.After 10,000 sweeping cycles,the mass activity still exhibits 5.7-fold enhancement compared to the corresponding commercial Pt/C.This work presents a new approach to delicately control the surface structure of ultrathin Pt-based NWs as advanced ORR catalysts.展开更多
基金Supported by a grant from the Natural Science Foundation of Yunnan Province(No.2011FZ129)
文摘Objective：Pulmonary fibrosis is a common pathological phenomena in lung cancer patients after chemotherapy or radiotherapy, which is a key factor hindering to transport ion of high concentrated drug to the lung tissue, peptide trans-porter has become targets of the rational design of peptides and peptide drug. The purpose of the study is to investigate the expression of PEPT2 mRNA in the lung of rats with bleomycin （BLM）-induced pulmonary fibrosis. Methods：Fifty healthy adult Spragne-Dawley rats were randomized into five groups, the rats in BLM 7d, 14d and 28d groups were treated with a single instil ation of 5 mg/kg of BLM, to induced pulmonary fibrosis models. On days 7, 14 and 28, the animals were kil ed by exsan-guination respectively. Normal saline （NS） group were treated by NS, on days 14, the animals were kil ed by exsanguinations. Control group were untreated. The lung samples were processed for light microscopy and determined the hydroxyproline （HYP） concentration. The expression of PEPT2 mRNA were measured by RT-PCR. PEPT2 cDNA fragments were tested by dideoxy chain termination. Results：Compared with control and NS group, HYP levels increased on day 7 of BLM group, but there was no statistical significant dif erence （P〉0.05）. HYP levels markedly increased on days 14 and 28 of BLM group, there was statistical significant dif erence （P〈0.01）. The morphological study showed that col agenous fiber proliferated on days 14 and 28 of BLM group, especial y on day 28, formed pulmonary fibrosis. There were no significant changes of pulmo-nary PEPT2 mRNA expression at dif erent groups （P〉0.05）. Conclusion：The pulmonary fibrosis models of SD rats can be induced by a single instil ation of 5 mg/kg of bleomycin on 28d. There were no significant changes of PEPT2 mRNA expression in the lung of rats with bleomycin-induced pulmonary fibrosis.
基金Supported by the National Natural Science Foundation of China (Grant No. 40772016)International Cooperation Program of MST of China (Grant No. 2007DFB20330)Cultural Relics Protected Program in Danjiangkou Reservoir Area of the South-to-North Water Diversion Project Held by Hubei Cultural Relics Bureau (Grant No. NK13)
文摘Traditional descriptive observation and advanced geometric morphometric are employed to study the morphological characteristics of Zhoukoudian mandibular premolars, and simultaneously with the specimens of Australopithecus, African early Homo, Homo erectus in other areas of Asia except Zhoukoudian, Europe Pleistocene fossil hominins, and recent Chinese (72 P3 and 69 P4 on the whole) being included as comparisons. Results suggest obvious evolutionary changes in the occlusal morphology and crown outline shape of mandibular premolars. Australopithecus P3s are found with extremely asymmetrical crown outlines, prominent mesial and distal vertical grooves on the buccal side, open foveas, and well-developed talonids, and P4s with similarly asymmetrical crown outlines, distinct mesial and distal vertical grooves on the buccal side, and "H" type of grooves combination. These characteristics are also expressed in the later hominin specimens, but with a decreased extent and lower frequency. The P3s of recent Chinese, slightly asymmetrical in the crown lingual contours, and P4s, basically symmetrical in the crown outlines, become faint or disappeared in the mesial or distal vertical buccal grooves, and decreased in the talonid size. It is suggested that Zhoukoudian mandibular premolars preserve some primitive traits of earlier hominins, including asymmetrical crown outlines and prominent mesial and distal vertical grooves of buccal side. Moreover, Zhoukoudian specimens exhibit obvious morphological variation between individuals, expressed mainly by aspects of mesial and distal vertical buccal grooves in various degrees, symmetry or asymmetry extent of crown lingual contour, relative location of polygon to the corresponding surrounding outline, as well as the relative size of talonid. When compared with Homo erectus from the other areas of Asia, Zhoukoudian specimens show their similarity with those of Xichuan and S-1, and at the same time great discrepancy with S-6 in the occlusal morphology and crown outline shape. When it comes to the relationship between specimens of Europe Pleistocene and Zhoukoudian, disparity is expressed in a stronger way than the corresponding similarity.
基金supported by grants from the Chinese National Key Research R&D Program(Nos.2017YFC0907601,2017YFC0907604)the CAMS Innovation Fund for Medical Sciences(CIFMS)(No.2019-I2M-2-008)the Fundamental Research Funds for CAMS&PUMC(No.2019PT330004).
文摘Background:Disease activity indices(DAIs)including disease activity score 28(DAS28),simplified disease activity index(SDAI),and clinical disease activity index(CDAI)have been widely used in clinical practice and research studies of rheumatoid arthritis(RA).The objective of our study was to evaluate the correlation and concordance among different DAIs in Chinese patients with RA.Methods:A cross-sectional study,including patients enrolled in the Chinese registry of rheumatoid arthritis from November 2016 to August 2018,was conducted.The correlations were evaluated using Spearman correlation coefficient and concordance with Bland-Altman plots,quadratic weighted kappa,and discordance rates in the crosstab.For other indices,the optimal cutoff points corresponding to SDAI remission were explored through receiver operating characteristic curve analysis.Results:A total of 30,501 patients were included,of whom 80.46%were women.Most individuals were with moderate disease activity or high disease activity.High correlations among DAS28-erythrocyte sedimentation rate(ESR)and DAS28-C-reactive protein(CRP),SDAI and CDAI were observed.Similarly,the weighted kappa value among the indices was high.In Bland-Altman plots,a positive difference between DAS28-ESR and DAS28-CRP was observed,with an absolute difference of>1.2 in 3079(10.09%)patients.In crosstab,approximately 30%of the patients were classified into different groups.Concordance values between SDAI remission and the optimal cutoff points of DAS28-ESR,DAS28-CRP,and CDAI were 3.06,2.37,and 3.20,respectively.Conclusions:Although DAIs had high correlations and weighted kappa values,the discordance between DAIs was significant in Chinese patients with RA.The four DAIs are not interchangeable.
基金This work was supported by the National Natural Science Foundation of China(NSFC)(No.51772142)Shenzhen Science and Technology Innovation Committee(Nos.KQJSCX20170328155428476 and KQTD2016053019134356)+1 种基金Development and Reform Commission of Shenzhen Municipality(Novel Nanomaterial Discipline Construction Plan)the China Postdoctoral Science Foundation(No.2018M641633).
文摘Pt-based ultrathin nanowires (NWs) are considered as one of the most intriguing catalysts for fuel cells.However,the delicate controllability of surface structure of ultrathin NWs to regulate their catalytic performances is still a challenge.Here,two kinds of one-nanometer-thick Pt-based NWs with smooth surfaces (S-NWs) and rough surfaces (R-NWs) are demonstrated,in which the combined use of hexadecyltrimethylammonium bromide and oleylamine plays an essential role,as they could form soft-templates to direct the growth of NWs.Due to its high-density of low-coordinated sites on the surface,Pt-based R-NWs exhibit higher oxygen reduction reaction (ORR) activities but lower stabilities than corresponding S-NWs.Notably,Pt0.78Ni0.22 R-NWs possess the highest mass activity (1.07 A-mgpt^-1) and specific activity (1.02 mA·cm^-2) among all Pt-based NWs.After 10,000 sweeping cycles,the mass activity still exhibits 5.7-fold enhancement compared to the corresponding commercial Pt/C.This work presents a new approach to delicately control the surface structure of ultrathin Pt-based NWs as advanced ORR catalysts.
