Analysis of functional hub genes indicates DLGAP5 is linked to lung adenocarcinoma prognosis

Introduction:The difficulty in treating lung adenocarcinoma(LUAD)is caused by a shortage of knowledge about the biological mechanisms and a lack of treatment choices.Objectives:The aim of this study was to identify a valuable molecular target for the treatment of LUAD.Methods:Using multiple databases,we screened for hub genes in LUAD using Cytoscape and explored the expression and prognosis of DLG associated protein 5(DLGAP5)in LUAD.We investigated the genetic variation,functional enrichment,and epigenetic activity of DLGAP5.Furthermore,we evaluated the relationship between the tumor microenvironment(TME)and DLGAP5.Results:Our study identified 10 hub genes in LUAD:CDC45,KIAA0101,DLGAP5,CDT1,NCAPG,CCNB1,CDCA5,CDC20,KIF11,and AURKA.We discovered that DLGAP5 was overexpressed and associated with poor prognosis in LUAD.DLGAP5 exhibited an overall genetic variation frequency of 2%,and its DNA promoter was hypomethylated in LUAD(p<0.05).The expression of DLGAP5 in LUAD showed a positive correlation with the majority of N6-methyladenosine(m6A)-methylation genes.Additionally,DLGAP5 was primarily associated with the cell cycle in LUAD.Notably,there was a significant favorable association between DLGAP5 and CD274,CTLA4,HAVCR2,and LAG3 in LUAD.Conclusion:DLGAP5 may be a therapeutic target for LUAD,as it affects cancer cells proliferation and development through the regulation of cell-cycle checkpoints and modulation of immune cell infiltration and immune checkpoints in the TME.

Effect of Trap Space Layout on Trapped Number of Spodoptera litura in Tobacco Field

[Objectives] This study was to find out the effect of trap space layout on the trapped number of Spodoptera litura in tobacco field. [Methods]From2015 to 2017,investigations were made using different hanging heights,distances( densities) and equidistant plane layout in the tobacco planting area in central Guizhou Province. [Results] The trapped number of S. litura was different at different hanging heights of the trap,and some had the difference reached the5% significant level in the trapped number. The optimum hanging height was 1. 5-2. 0 m. The trapping effect was the best at the field layout distance of20. 0-30. 0 m( density of 5-8 traps/hm^2). Under the conditions of even distribution at the equal distance between traps of 40.0 m in the field,the accumulated trapped number of S. litura from April to August was in the order of outer ring > central ring > inner ring,presenting the zonal distribution. The difference in the trapped numbers was significant among different rings,reaching the 5% significant level. [Conclusions]This study provided theoretical bases for the physiochemical control of S. litura in tobacco field.