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基于Sentinel-2影像的东寨港国家级自然保护区景观格局变化及其驱动力研究
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作者 李庆庆 李诗菲 +3 位作者 张悦 许道艳 熊德琪 廖国祥 《海洋开发与管理》 2024年第3期91-101,共11页
文章以东寨港国家级自然保护区及1 km外围地带为研究区域,选取2017—2021年的Sentinel-2遥感影像数据,采用人机交互方式解译景观类型,计算景观格局指数,进而对比分析5年期间保护区内外的景观格局变化情况及其主要驱动力。结果表明:(1)... 文章以东寨港国家级自然保护区及1 km外围地带为研究区域,选取2017—2021年的Sentinel-2遥感影像数据,采用人机交互方式解译景观类型,计算景观格局指数,进而对比分析5年期间保护区内外的景观格局变化情况及其主要驱动力。结果表明:(1)在保护区内,红树林湿地面积呈现上升趋势,林地面积有所减少,养殖水面面积显著减少;保护区景观连通性较好、碎度较低,其中红树林为优势景观。(2)在外围地带,红树林湿地面积有所增加,林地面积有所减少,养殖水面和建筑面积均减少,但耕地面积有所增加;与保护区相比,景观破碎化程度较高、连接度与整体性较差。(3)红树林生态保护修复活动是保护区景观格局发生显著变化的主要驱动力,而养殖、建筑等人类活动是改变外围地带景观格局的主要因素。研究结果对于优化保护区的保护管理和加强外围地带的人类活动管控具有参考价值。 展开更多
关键词 东寨港 自然保护区 Sentinel-2影像 景观格局 驱动力
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Research on the risk-based model for regional emergency resource allocation for ship-source oil spill 被引量:3
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作者 ZHANG Chunchang AN Wei +2 位作者 xiong deqi LIU Baozhan SONG Shasha 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2018年第11期133-138,共6页
The key point for rational allocation of emergency resources is to match the oil spill response capacity with the risk of oil spill. This paper proposes an innovative risk-based model for quantitative regional emergen... The key point for rational allocation of emergency resources is to match the oil spill response capacity with the risk of oil spill. This paper proposes an innovative risk-based model for quantitative regional emergency resource allocation, which comprehensively analyzes the factors such as oil spill probability, hazard consequences, oil properties, weathering process and operation efficiency, etc. The model calculates three major resources, i.e., mechanical recovery, dispersion and absorption, according to the results of risk assessment. In a field application in Xiaohu Port, Guangzhou, China, and the model achieved scientific and rational allocation of emergency resources by matching the assessed risk with the regional capacity, and allocating emergency resources according to capability target. The model is considered to be beneficial to enhancing the resource efficiency and may contribute to the planning of capacity-building programs in high-risk areas. 展开更多
关键词 oil spill risk oil spill quantity PROBABILITY emergency capability
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蜘蛛香环烯醚萜类成分对大鼠脊髓损伤后氧化应激的影响 被引量:4
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作者 黄姣娟 王文春 +5 位作者 熊德启 卢豪 向武 赵小华 周蕊寒 张安仁 《康复学报》 CSCD 2019年第3期27-32,共6页
目的:分别从组织学和分子生物学层面观察蜘蛛香环烯醚萜类成分对大鼠脊髓损伤后氧化应激反应的影响。方法:选取体质量(250±30)g的雄性SD大鼠60只,采用随机数字表法分为假手术组、模型组和蜘蛛香环烯醚萜类成分治疗组(简称治疗组),... 目的:分别从组织学和分子生物学层面观察蜘蛛香环烯醚萜类成分对大鼠脊髓损伤后氧化应激反应的影响。方法:选取体质量(250±30)g的雄性SD大鼠60只,采用随机数字表法分为假手术组、模型组和蜘蛛香环烯醚萜类成分治疗组(简称治疗组),每组20只。采用医用动脉瘤夹建立大鼠脊髓损伤模型,假手术组大鼠只进行椎板切除而不进行脊髓损伤。造模成功2h后,治疗组给予灌胃10mg/kg蜘蛛香环烯醚萜类成分溶液,假手术组、模型组给予灌胃等体积羧甲基纤维素钠(CMC-Na)溶液,1次/d。3d后处死大鼠,酶联免疫吸附法(ELISA)检测血清中氧化应激因子超氧化物歧化酶(SOD)和丙二醛(MDA)含量;生化试剂盒检测脊髓组织谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)活力;Westernblot法观察凋亡相关蛋白:胱天蛋白酶-3(Caspase-3)、B细胞淋巴瘤-2(Bcl-2)相关X蛋白(Bax)和Bcl-2的表达水平;苏木精-伊红(HE)染色观察脊髓组织病理变化;末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法(TUNEL法)观察脊髓组织神经细胞凋亡情况。结果:ELISA检测结果显示,与模型组比较,治疗组血清中SOD含量显著增加(P<0.05),MDA含量显著降低(P<0.01);试剂盒检测结果显示,与模型组比较,治疗组显著增加了脊髓组织中GSH-PX(P<0.05)和CAT(P<0.01)抗氧化酶活力;Westernblot结果显示,与模型组比较,治疗组促凋亡蛋白Caspase-3、Bax表达明显降低(P<0.05),抗凋亡蛋白Bcl-2表达明显增加(P<0.05),且Bax/Bcl-2的比例明显下降(P<0.01)。HE染色结果显示,假手术组中脊髓组织灰质及白质结构正常,神经元结构清晰,未见显著的脊髓受损;与假手术组比较,模型组大鼠的脊髓组织中观察到脊髓结构严重受损,脊髓中央部的灰质和位于周围部的白质结构模糊,大量神经元变性、坏死,细胞结构被破坏,胞核皱缩或溶解消失并伴有不同程度的中性粒细胞浸润及大量小胶质细胞增生;与模型组比较,治疗组脊髓结构明显改善,脊髓组织病变相对较轻,小区域的神经元坏死,少量的嗜酸性细胞增多,小胶质细胞浸润,且组织病理学评分低于模型组(P<0.05)。同时TUNEL法检测结果显示,假手术未看到明显的棕黄色凋亡神经细胞,而模型组中观察到明显的棕黄色的凋亡神经细胞,与模型组比较,治疗组凋亡神经细胞数明显减少,差异具有统计学意义(P<0.05)。结论:蜘蛛香环烯醚萜类成分对脊髓损伤具有神经保护作用,其机制可能与减轻脊髓损伤后氧化应激反应有关。 展开更多
关键词 脊髓损伤 蜘蛛香环烯醚萜类成分 氧化应激 神经保护
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Comparison of toxicity effects of fuel oil treated by different dispersants on marine medaka(Oryzias melastigma) embryo
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作者 GAO Xiang DING Guanghui +1 位作者 LI Xishan xiong deqi 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2018年第11期123-132,共10页
This study aims to evaluate the subacute toxic effects of oil under different treatments on marine organism by simulating natural contaminative processes. In this study, 120# (RMD15) fuel oil was selected as the pol... This study aims to evaluate the subacute toxic effects of oil under different treatments on marine organism by simulating natural contaminative processes. In this study, 120# (RMD15) fuel oil was selected as the pollutant and marine medaka (Oryzias melastigma) embryos as the experimental organism. The developmental toxicity of different volume concentrations (0.05%, 0.2%, 1% and 5%) of water-accommodated fractions, biologically-enhanced water-accommodated fractions, and chemically-enhanced water-accommodated fractions on the embryos in different exposure time (8, 15 and 22 d) were compared and the content of relevant polycyclic aromatic hydrocarbons (PAHs) was studied (in dispersion and in vivo). The subacute toxic effects were assessed in terms of antioxidant activities of enzymes (superoxide dismutase, catalase and glutathione S-transferase) and the blue sac disease (BSD) indexes.The results showed that the BSD indexes of the treatment groups were significantly higher than the respective control groups and showed positive correlations with both concentration and exposure time. The experiments with three antioxidant enzymes indicated that enzymatic activities of the embryos changed dramatically under the oxidation stress of petroleum hydrocarbons, especially after adding the dispersants. With the increase of petroleum hydrocarbon concentration and exposure time, the three enzymes showed different degrees of induction and inhibition effects. 展开更多
关键词 marine medaka petroleum hydrocarbon oil dispersant antioxidant enzymes 120# fuel oil
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