Density functional theory is used to investigate the complexes structures and properties of poly(vinyl ethylene carbonate)(PVEC/LiTFSI)and poly(vinylene carbonate)(PVCA/LiDFOB)electrolytes containing five-membered rin...Density functional theory is used to investigate the complexes structures and properties of poly(vinyl ethylene carbonate)(PVEC/LiTFSI)and poly(vinylene carbonate)(PVCA/LiDFOB)electrolytes containing five-membered ring carbonate groups under the polymer/Li^(+)model and the polymer/lithium salt model.In addition,the calculated and experimental values of the oxidation potentials of the two electrolytes were compared,and the reasons for the differences in the oxidation potentials of the two electrolytes are elucidated.The calculation results show that the PVEC/LiTFSI has more free ion structures and diverse coordination structures compared to PVCA/LiDFOB electrolyte.This provides a reasonable theoretical explanation for its higher ionic conductivity and lower cation mobility number.The PVEC/LiTFSI electrolyte has a lower oxidation potential compared to the PVCA/LiDFOB electrolyte,which is attributed to the proton transfer that occurs during its oxidation.展开更多
目的评估生物可降解镁合金材料在肩袖撕裂修复中促进肌腱-骨愈合的效果,并探讨其潜在的生物学机制。方法取8周龄SD大鼠48只,随机分为A、B、C 3组。A、B组大鼠制备肩袖撕裂模型后分别通过镁合金缝线或Vicryl Plus 4-0可吸收缝线进行修复,...目的评估生物可降解镁合金材料在肩袖撕裂修复中促进肌腱-骨愈合的效果,并探讨其潜在的生物学机制。方法取8周龄SD大鼠48只,随机分为A、B、C 3组。A、B组大鼠制备肩袖撕裂模型后分别通过镁合金缝线或Vicryl Plus 4-0可吸收缝线进行修复,C组仅行皮下切开和缝合。术后1、2周取A、B组内脏行HE染色评估镁合金的安全性;术后2、4、8、12周取3组冈上肌腱-肱骨近端标本,其中4、12周标本行大体观察,4、8、12周标本行生物力学检测A、B组愈合部位的极限载荷及刚度;2、4、12周标本行以下检测:Micro-CT评估A、B组骨隧道形成情况,标本脱钙切片后行HE染色和Masson染色观察肌腱-骨界面纤维软骨再生情况,Goldner三色染色评估钙化情况,免疫组织化学染色检测肌腱-骨界面血管VEGF、BMP-2等成血管因子及成骨因子的表达,免疫荧光染色通过检测精氨酸酶1、整合素β2亚基观察肌腱-骨界面巨噬细胞M1、M2极化情况,通过实时荧光定量PCR分析磷脂酰肌醇3激酶(phosphatidylinositede 3-kinase,PI3K)/蛋白激酶B(protein kinase B,AKT)信号通路在肌腱-骨愈合中的作用机制。结果内脏组织HE染色示,镁合金降解过程中释放的镁离子对心脏、肝脏、脾脏、肺、肾脏等器官未产生明显毒性作用,其生物安全性良好。冈上肌腱-肱骨近端标本组织学染色示,A组肌腱-骨界面处的纤维软骨再生恢复较早,且纤维软骨数量明显多于B组,提示镁合金材料对肌腱-骨界面修复具有积极作用。Micro-CT分析示,A组骨隧道形成快于B组,进一步验证了镁合金材料对骨愈合的促进作用。生物力学测试示,A组的极限载荷均高于B组,4周时A组刚度高于B组,表明A组肌腱-骨界面修复后的组织承载能力更强,镁合金具有改善肌腱-骨愈合的潜力。免疫组织化学染色示,肌腱-骨愈合早期VEGF和BMP-2表达显著上调,提示镁合金能够有效促进血管生成与骨形成,从而加速愈合进程。免疫荧光染色示,修复早期镁离子通过调节巨噬细胞的极化状态,促进其向M2型巨噬细胞极化,发挥显著抗炎作用。实时荧光定量PCR检测表明,镁离子可以通过调控PI3K/AKT信号通路促进肌腱-骨愈合过程。结论生物可降解镁合金材料通过调控PI3K/AKT信号通路,加速大鼠肩袖撕裂修复中肌腱-骨界面的纤维软骨再生和钙化,显著提升肌腱-骨愈合效果。展开更多
文摘Density functional theory is used to investigate the complexes structures and properties of poly(vinyl ethylene carbonate)(PVEC/LiTFSI)and poly(vinylene carbonate)(PVCA/LiDFOB)electrolytes containing five-membered ring carbonate groups under the polymer/Li^(+)model and the polymer/lithium salt model.In addition,the calculated and experimental values of the oxidation potentials of the two electrolytes were compared,and the reasons for the differences in the oxidation potentials of the two electrolytes are elucidated.The calculation results show that the PVEC/LiTFSI has more free ion structures and diverse coordination structures compared to PVCA/LiDFOB electrolyte.This provides a reasonable theoretical explanation for its higher ionic conductivity and lower cation mobility number.The PVEC/LiTFSI electrolyte has a lower oxidation potential compared to the PVCA/LiDFOB electrolyte,which is attributed to the proton transfer that occurs during its oxidation.