目的:观察肿瘤坏死因子α诱导蛋白2(TNF-α-induced protein 2,TNFAIP2)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达以及对细胞增殖、凋亡、迁移和侵袭能力的影响。方法:RT-PCR检测NSCLC中TNFAIP2 m RNA表达情况;免疫组...目的:观察肿瘤坏死因子α诱导蛋白2(TNF-α-induced protein 2,TNFAIP2)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达以及对细胞增殖、凋亡、迁移和侵袭能力的影响。方法:RT-PCR检测NSCLC中TNFAIP2 m RNA表达情况;免疫组化检测NSCLC中TNFAIP2蛋白表达情况。构建TNFAIP2基因短发夹RNA(short hairpin RNA,shRNA)干扰慢病毒载体(shRNA-TNFAIP2)和阴性对照慢病毒载体(shRNA-NC)分别感染H1299细胞后采用RT-PCR和Western blot检测感染效率。用Transwell实验检测细胞的迁移、侵袭能力;用CCK-8检测细胞的增殖能力;用流式细胞术检测细胞的凋亡能力;用Western blot检测细胞蛋白E-cadherin、N-cadherin、Vimentin表达的变化。结果:TNFAIP2 m RNA和蛋白质在NSCLC癌组织中的表达明显高于癌旁组织中的表达。慢病毒下调TNFAIP2表达后,shRNA-TNFAIP2组细胞迁移数(170±11)较shRNA-NC组(329±31)明显减少(P=0.001);侵袭细胞数(75±10)较shRNA-NC组(135±8)明显减少(P=0.001)。CCK-8结果提示,shRNATNFAIP2组的吸光度值明显低于shRNA-NC组(P=0.000);流式细胞术结果显示,shRNA-TNFAIP2组的凋亡率(18.730±0.490)%明显高于shRNA-NC组(6.570±0.870)%(P=0.000);Western blot结果显示,shRNA-TNFAIP2组较shRNA-NC组细胞蛋白N-cadherin、Vimentin表达降低,E-cadherin表达增加。结论:TNFAIP2在NSCLC中存在高表达,下调能够抑制细胞的增殖、迁移和侵袭能力,促进细胞凋亡,逆转上皮间质转化。展开更多
Five nearly E-W-trending alkali-rich igneous rock belts are distributed from north to south in northern Xinjiang, and they are composed mainly of riebeckite, K-feldspar granite and high-K and medium-K calc-alkaline vo...Five nearly E-W-trending alkali-rich igneous rock belts are distributed from north to south in northern Xinjiang, and they are composed mainly of riebeckite, K-feldspar granite and high-K and medium-K calc-alkaline volcanic rocks and shoshonite. They were mainly emplaced or erupted between the Carboniferous and Permian. The compositions of Sr, Nd, Pb, and O isotopes imply that their principal resource materials are derived from the upper mantle or juvenile crust, indicating obvious continental growth in the Phanerozoic. The trace element association implies that their tectonic settings are within plate and volcanic arc for alkali-rich granites, and post-collisional are, late oceanic are and continental are for alkali-rich volcanic rocks. An archipelago model was suggested for the tectonic evolution in northern Xinjiang. It can be named the central-Asia-type orogeny, which is different from the so called circum-Pacific ocean-continent-type tectonics or the Alpine-Himalayan continent-continent-collisional orogeny.展开更多
文摘目的:观察肿瘤坏死因子α诱导蛋白2(TNF-α-induced protein 2,TNFAIP2)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达以及对细胞增殖、凋亡、迁移和侵袭能力的影响。方法:RT-PCR检测NSCLC中TNFAIP2 m RNA表达情况;免疫组化检测NSCLC中TNFAIP2蛋白表达情况。构建TNFAIP2基因短发夹RNA(short hairpin RNA,shRNA)干扰慢病毒载体(shRNA-TNFAIP2)和阴性对照慢病毒载体(shRNA-NC)分别感染H1299细胞后采用RT-PCR和Western blot检测感染效率。用Transwell实验检测细胞的迁移、侵袭能力;用CCK-8检测细胞的增殖能力;用流式细胞术检测细胞的凋亡能力;用Western blot检测细胞蛋白E-cadherin、N-cadherin、Vimentin表达的变化。结果:TNFAIP2 m RNA和蛋白质在NSCLC癌组织中的表达明显高于癌旁组织中的表达。慢病毒下调TNFAIP2表达后,shRNA-TNFAIP2组细胞迁移数(170±11)较shRNA-NC组(329±31)明显减少(P=0.001);侵袭细胞数(75±10)较shRNA-NC组(135±8)明显减少(P=0.001)。CCK-8结果提示,shRNATNFAIP2组的吸光度值明显低于shRNA-NC组(P=0.000);流式细胞术结果显示,shRNA-TNFAIP2组的凋亡率(18.730±0.490)%明显高于shRNA-NC组(6.570±0.870)%(P=0.000);Western blot结果显示,shRNA-TNFAIP2组较shRNA-NC组细胞蛋白N-cadherin、Vimentin表达降低,E-cadherin表达增加。结论:TNFAIP2在NSCLC中存在高表达,下调能够抑制细胞的增殖、迁移和侵袭能力,促进细胞凋亡,逆转上皮间质转化。
基金Thanks are due to the financial support from the National 305 Project of Xinjiang(859020601,859020607,959160302)the National ClimbingProgram of China(A-30,95-Y-25).
文摘Five nearly E-W-trending alkali-rich igneous rock belts are distributed from north to south in northern Xinjiang, and they are composed mainly of riebeckite, K-feldspar granite and high-K and medium-K calc-alkaline volcanic rocks and shoshonite. They were mainly emplaced or erupted between the Carboniferous and Permian. The compositions of Sr, Nd, Pb, and O isotopes imply that their principal resource materials are derived from the upper mantle or juvenile crust, indicating obvious continental growth in the Phanerozoic. The trace element association implies that their tectonic settings are within plate and volcanic arc for alkali-rich granites, and post-collisional are, late oceanic are and continental are for alkali-rich volcanic rocks. An archipelago model was suggested for the tectonic evolution in northern Xinjiang. It can be named the central-Asia-type orogeny, which is different from the so called circum-Pacific ocean-continent-type tectonics or the Alpine-Himalayan continent-continent-collisional orogeny.
