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Effect of Eliminated the Breeding Pigs with Unqualified CSFV Antibody on Sows' Reproductive Performance and Pigs' Growth Performance
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作者 xiu jin-sheng WU Shun-yi +5 位作者 ZHOU Lun-jiang YE Yao-hui YU Dao-jin CHEN Ru-jing WANGLong-bai ZHANG Xin-liang 《Agricultural Science & Technology》 CAS 2011年第5期728-732,共5页
[Objective] The aim was to explore the effect of eliminated the breeding pigs with unqualified CSFV antibody on sow's reproductive performance and pig's growth performance so as to provide a scientific basis f... [Objective] The aim was to explore the effect of eliminated the breeding pigs with unqualified CSFV antibody on sow's reproductive performance and pig's growth performance so as to provide a scientific basis for the eliminating of the breeding pigs with unqualified CSFV antibody in large-scale pig farms.[Method] Serum samples from three scale pig farms were collected and tested with HerdChek CSFV-ELISA kit,then provided a certain scientific basis for scale pig farm to eliminate the unqualified(antibody blocking rate<50%)breeders after booster immunization.[Result ] After eliminating the breeding pigs with unqualified CSFV antibody,the average litter number of piglet in three pig farms(A,B,C)were increased by 0.29(P<0.05),0.40(P<0.01),0.39(P<0.01)respectively;the average number of survival piglets were increased by 0.54(P<0.01),0.35(P<0.01),0.62(P<0.01)respectively;the average litter number of weaned piglets were increased by 0.65(P<0.01),0.71(P<0.01),0.81(P<0.01)respectively.The difference in weight gain of piglet at 30-60 d of age was extremely significant(P<0.01),but inconspicuousness for the swine pregnancy rate,the survival rate of weaned piglet and piglet at 30-60 d of age.[Conclusion] The eliminating the breeding pigs with unqualified CSFV antibody can significantly improve the performance of breeder and piglets. 展开更多
关键词 断奶仔猪 猪瘟抗体 生长性能 繁殖性能 母猪 检测试剂盒 病毒抗体 种猪
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猪伪狂犬病病毒MQ18变异株的分离鉴定 被引量:6
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作者 曾显成 池雪林 +4 位作者 潘启东 陈珍珍 林琳 修金生 江斌 《中国兽医科学》 CAS CSCD 北大核心 2019年第8期1025-1033,共9页
为了解福建省猪伪狂犬病病毒(PRV)野毒株的病原学特性及分子遗传变异情况,本研究用羊胚胎鼻甲细胞(OFTu)从疑似暴发伪狂犬病猪场死亡的育肥猪中分离到1株病毒,该病毒能够产生典型的PRV细胞病变,病毒液接种小鼠(0.1 mL/只)及成年兔(0.5 ... 为了解福建省猪伪狂犬病病毒(PRV)野毒株的病原学特性及分子遗传变异情况,本研究用羊胚胎鼻甲细胞(OFTu)从疑似暴发伪狂犬病猪场死亡的育肥猪中分离到1株病毒,该病毒能够产生典型的PRV细胞病变,病毒液接种小鼠(0.1 mL/只)及成年兔(0.5 mL/只)在48~60 h死亡,均表现出典型的伪狂犬病症状。经PCR试验、电镜观察及特异性血清间接免疫荧光试验鉴定为伪狂犬病病毒,命名为MQ18株;并对其进行病毒效价的测定及gC、gE基因核苷酸和氨基酸序列差异性分析。结果显示,分离株TCID50为10-7.71/0.1 mL;将其gC和gE基因序列与国内外17个PRV参考毒株进行同源性比较,核苷酸序列同源性分别为93.5%~99.9%和97.8%~100%,氨基酸序列同源性分别为88.7%~99.8%和95.7%~100%;分子遗传进化树分析显示,gC和gE基因均与国内近6年分离的PRV变异株集聚在一起,属于同一个进化分支;与经典株相比,gC基因编码的氨基酸序列在第63~69位有1个AAASTPA的连续7个氨基酸插入,gE基因编码的氨基酸序列分别在第48位和第496位有1个天冬氨酸(D)的插入,与PRV变异株变异位点相一致。以上结果证实,从发病猪群中分离到1株PRV变异株,本研究结果为选择合适疫苗来防控猪伪狂犬病以及病原学研究提供了参考。 展开更多
关键词 伪狂犬病病毒 变异 分离鉴定 序列分析
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