Objective To investigate the occurrence of important foodbome pathogens in shellstock Pacific oysters in the food markets in South China. Methods From July 2007 to June 2008, retail oysters were collected in different...Objective To investigate the occurrence of important foodbome pathogens in shellstock Pacific oysters in the food markets in South China. Methods From July 2007 to June 2008, retail oysters were collected in different seasons from South China and analyzed for the prevalence and levels of Listeria monocytogenes, Vibrio vulnificus and Vibrio parahaemolyticus. Results None of L. monocytogenes could be detected in any of the 202 oyster samples tested, while E vulnificus and E parahaemolyticus could be detected in 67 （54.9%） and 109 （89.3%） of the 122 oyster samples analyzed, respectively, with an MPN （most probable number） value greater than or equal to 3. V. wdnificus and Eparahaemolyticus with a more than 102 MPN/g were found in 36 （29.5%） and 59 （48.4%） of the 122 oyster samples, respectively. The tdh and trh genes were detected in 4 （0.3%） and 8 （0.6%） of the 1 349 V. parahaemolyticus isolates, respectively. Of the 122 samples, 4 （3.3%） was positive for either tdh or trh. The levels of E vulnificus and total V. parahaemolyticus in oysters in South China varied in different seasons. Conclusion V. vulnificus and pathogenic V. parahaemolyticus are frequently found in oysters in south China, which may pose a potential threat to public health. Data presented here will be useful for the microbiological risk assessment in oysters in China.展开更多
Metallocene based polyethylenes were prepared by SMOPEC's 'metallocene adduct' technology in a gas phase fluidized bed model reactor. The C-13-NMR spectra of ethylene/1-butene (S-34) and ethylene/1-hexene(...Metallocene based polyethylenes were prepared by SMOPEC's 'metallocene adduct' technology in a gas phase fluidized bed model reactor. The C-13-NMR spectra of ethylene/1-butene (S-34) and ethylene/1-hexene(S-43) copolymers were studied in a manner analogous to that established by Hsieh and Cheng. The comonomer sequence distributions of copolymer samples were obtained. The results show that these metallocene based copolymers contain a small amount of butene and hexene, and the EE and EEE sequences are dominant.展开更多
Objective:To assess the relationship between the severity of atopic dermatitis(AD)in children of varying weight categories and their serum 25-hydroxyvitamin D[25(OH)D]levels.Methods:The study population comprised 899 ...Objective:To assess the relationship between the severity of atopic dermatitis(AD)in children of varying weight categories and their serum 25-hydroxyvitamin D[25(OH)D]levels.Methods:The study population comprised 899 patients with AD and 854 age-and sex-matched controls.The Mann-Whitney U test and Kruskal-Wallis H tests were used to assess differences between groups,and Spearman correlation analysis was used to test correlation.Results:The 25(OH)D level in the AD group was M(Q_(25),Q_(75)),24.0(19.7,28.4)ng/mL,which was significantly lower than the control group(26.4[23.6,29.9]ng/mL;Z=-3.34,P=0.001).25(OH)D levels in children with AD were negatively correlated with body mass index(r=-0.30,P<0.001),Severity scoring of Atopic Dermatitis(SCORAD;r=-0.14,P<0.001),total immunoglobulin E(r=-0.13,P<0.001),and eosinophil(r=-0.08,P=0.017).There were statistically significant differences in 25(OH)D(H=18.46,P<0.001),total immunoglobulin E(H=9.13,P=0.010),eosinophil(H=67.17,P<0.001),and SCORAD(H=10.49,P=0.005)among groups with different body mass index classification.The 25(OH)D levels were 22.3(17.5,27.1)ng/mL in the overweight AD group and 22.3(17.6,25.7)ng/mL in the obese AD group,which were significantly lower than those in the normal-weight AD group(24.7[20.4,25.5]ng/mL;P=0.003,P=0.004).25(OH)D levels were negatively correlated with SCORAD in obese AD patients(r=-0.25,P=0.010).Conclusion:Vitamin D insufficient or deficient is obvious in children AD patients.The 25(OH)D levels in the overweight/obese AD group are significantly lower than those in the normal-weight AD group.Vitamin D level is negatively correlated with SCORAD in obese children with AD.展开更多
Calf spleen extract （CSE） has been clinically used as an adjuvant agent in malignant tumor therapy. It can improve the physical status of patients. However, its mechanism of action remains relatively unclear. In thi...Calf spleen extract （CSE） has been clinically used as an adjuvant agent in malignant tumor therapy. It can improve the physical status of patients. However, its mechanism of action remains relatively unclear. In this study, we investigated the effect of CSE on leucopenia in mice and on promyelocytic leukemia cells （HL60）. CSE in 10 mL/kg （2.5 mg bioactive polypeptides and 190 pg ribose/mL） significantly increased leukocyte numbers in leucopenia mice. The effect on neutrophil numbers, among all leukocytes, was the most evident. CSE stimulated the proliferation of bone marrow cells in vitro and decreased the GM-CSF level in serum. In addition, CSE significantly reduced the viability of HL60 cells, decreased the production of lactate and adenosine triphosphate （ATP） of these cells. CSE induced the apoptosis and S-phase arrest in HL60 cells. In conclusion, CSE can enhance leukocyte numbers, which may be attributed to its direct stimulatory effect on bone marrow cells. CSE is an inhibitor ofpromyelocytic leukemia cell viability, which may be attributed to the induction of the apoptosis, the arrest of cell cycle and the inhibition of the glycolysis of cells.展开更多
基金supported by grants from the Key Projects in the National Science and Technology Pillar Program of China in the Eleventh Five-year Plan Period (Contract No. 2006BAK02A15)National Natural Science Foundation of China (Contract No. 30600499)
文摘Objective To investigate the occurrence of important foodbome pathogens in shellstock Pacific oysters in the food markets in South China. Methods From July 2007 to June 2008, retail oysters were collected in different seasons from South China and analyzed for the prevalence and levels of Listeria monocytogenes, Vibrio vulnificus and Vibrio parahaemolyticus. Results None of L. monocytogenes could be detected in any of the 202 oyster samples tested, while E vulnificus and E parahaemolyticus could be detected in 67 （54.9%） and 109 （89.3%） of the 122 oyster samples analyzed, respectively, with an MPN （most probable number） value greater than or equal to 3. V. wdnificus and Eparahaemolyticus with a more than 102 MPN/g were found in 36 （29.5%） and 59 （48.4%） of the 122 oyster samples, respectively. The tdh and trh genes were detected in 4 （0.3%） and 8 （0.6%） of the 1 349 V. parahaemolyticus isolates, respectively. Of the 122 samples, 4 （3.3%） was positive for either tdh or trh. The levels of E vulnificus and total V. parahaemolyticus in oysters in South China varied in different seasons. Conclusion V. vulnificus and pathogenic V. parahaemolyticus are frequently found in oysters in south China, which may pose a potential threat to public health. Data presented here will be useful for the microbiological risk assessment in oysters in China.
文摘Metallocene based polyethylenes were prepared by SMOPEC's 'metallocene adduct' technology in a gas phase fluidized bed model reactor. The C-13-NMR spectra of ethylene/1-butene (S-34) and ethylene/1-hexene(S-43) copolymers were studied in a manner analogous to that established by Hsieh and Cheng. The comonomer sequence distributions of copolymer samples were obtained. The results show that these metallocene based copolymers contain a small amount of butene and hexene, and the EE and EEE sequences are dominant.
基金supported by the Key Project of Social Development in Jiangsu Province(No.BE2020632)National Natural Science Foundation of China(Nos.82073446 and 82103735)+1 种基金CAMS Innovation Fund for Medical Sciences(No.2021-I2M-1-059)Nanjing Incubation Program for National Clinical Research Center(No.2019060001)
文摘Objective:To assess the relationship between the severity of atopic dermatitis(AD)in children of varying weight categories and their serum 25-hydroxyvitamin D[25(OH)D]levels.Methods:The study population comprised 899 patients with AD and 854 age-and sex-matched controls.The Mann-Whitney U test and Kruskal-Wallis H tests were used to assess differences between groups,and Spearman correlation analysis was used to test correlation.Results:The 25(OH)D level in the AD group was M(Q_(25),Q_(75)),24.0(19.7,28.4)ng/mL,which was significantly lower than the control group(26.4[23.6,29.9]ng/mL;Z=-3.34,P=0.001).25(OH)D levels in children with AD were negatively correlated with body mass index(r=-0.30,P<0.001),Severity scoring of Atopic Dermatitis(SCORAD;r=-0.14,P<0.001),total immunoglobulin E(r=-0.13,P<0.001),and eosinophil(r=-0.08,P=0.017).There were statistically significant differences in 25(OH)D(H=18.46,P<0.001),total immunoglobulin E(H=9.13,P=0.010),eosinophil(H=67.17,P<0.001),and SCORAD(H=10.49,P=0.005)among groups with different body mass index classification.The 25(OH)D levels were 22.3(17.5,27.1)ng/mL in the overweight AD group and 22.3(17.6,25.7)ng/mL in the obese AD group,which were significantly lower than those in the normal-weight AD group(24.7[20.4,25.5]ng/mL;P=0.003,P=0.004).25(OH)D levels were negatively correlated with SCORAD in obese AD patients(r=-0.25,P=0.010).Conclusion:Vitamin D insufficient or deficient is obvious in children AD patients.The 25(OH)D levels in the overweight/obese AD group are significantly lower than those in the normal-weight AD group.Vitamin D level is negatively correlated with SCORAD in obese children with AD.
文摘Calf spleen extract （CSE） has been clinically used as an adjuvant agent in malignant tumor therapy. It can improve the physical status of patients. However, its mechanism of action remains relatively unclear. In this study, we investigated the effect of CSE on leucopenia in mice and on promyelocytic leukemia cells （HL60）. CSE in 10 mL/kg （2.5 mg bioactive polypeptides and 190 pg ribose/mL） significantly increased leukocyte numbers in leucopenia mice. The effect on neutrophil numbers, among all leukocytes, was the most evident. CSE stimulated the proliferation of bone marrow cells in vitro and decreased the GM-CSF level in serum. In addition, CSE significantly reduced the viability of HL60 cells, decreased the production of lactate and adenosine triphosphate （ATP） of these cells. CSE induced the apoptosis and S-phase arrest in HL60 cells. In conclusion, CSE can enhance leukocyte numbers, which may be attributed to its direct stimulatory effect on bone marrow cells. CSE is an inhibitor ofpromyelocytic leukemia cell viability, which may be attributed to the induction of the apoptosis, the arrest of cell cycle and the inhibition of the glycolysis of cells.
