Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily.In this study,we identified two novel members of the Smad family,termed as AmphiSmad1/5/8 and AmphiSmad4,from Chinese amp...Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily.In this study,we identified two novel members of the Smad family,termed as AmphiSmad1/5/8 and AmphiSmad4,from Chinese amphioxus.Both AmphiSmad1/5/8 and AmphiSmad4 showed a typical domain structure of Smad proteins consisting of conserved MH1 and MH2 domains.Phylogenetic analysis placed AmphiSmad1/5/8 in the Smad1,5 and 8 subgroup of the R-Smad subfamily,and AmphiSmad4 in the Co-Smad subfamily.The spatial and temporal gene expression patterns of AmphiSmad1/5/8 and AmphiSmad4 showed that they may be involved in the embryonic development of notochord,myotome and alimentary canal,and may help to establish the specification of dorsal-ventral axis of amphioxus.Moreover,AmphiSmad1/5/8 and AmphiSmad4 showed extensive distribution in all adult tissues examined,suggesting that these two genes may play important roles in the morphogenesis of a variety of tissues especially notochord and gonad.展开更多
现代生命科学的兴起引发了学术界在思维方式及对生命本质认知上的巨大变革,随着现代科学技术的快速发展,以精准医学和系统生物学为代表的现代生物医学,在认识人类生命的范畴上,不断地与中医关于生命认识的整体观和天人合一的思维接近。...现代生命科学的兴起引发了学术界在思维方式及对生命本质认知上的巨大变革,随着现代科学技术的快速发展,以精准医学和系统生物学为代表的现代生物医学,在认识人类生命的范畴上,不断地与中医关于生命认识的整体观和天人合一的思维接近。二者虽属不同的学术思维体系,但随着全球人类对于生命呵护需求的趋同化和高质化,中医和西医最终走向融合是未来发展的必然趋势。为了推动这种融合更加有序和快速形成,在此试图提出一个崭新的学科概念:中医生命科学(Life Science From the Perspective of Chinese Medicine)。此概念涵盖四大要素,即物质、能量、信息、时空。通过深入挖掘中医生命观与现代科学的结合点,以期推动基于中医生命科学的新医学发展。起始的具体研究方向分别是中医生命的系统物质基础,中医生命的能量、信息、时空变化与规律,中医药大数据的智慧凝练,中医生命医学工程的设备开发。使中医在现代的环境下能够更加有效地服务于人类健康事业,实现中西医学整合框架下的融合,为新医学的建立提供生命科学的基础支撑。展开更多
Limited genetic information is available concerning the polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. The aim of this study is to identify the allelic frequencies of the chemokine and chemo...Limited genetic information is available concerning the polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. The aim of this study is to identify the allelic frequencies of the chemokine and chemokine receptor genes in the Chinese mainland. Genomic DNA samples extracted from whole blood of 2318 subjects were analyzed by using PCR or PCR/restriction fragment length polymorphism (RFLP) assays, and further confirmed by direct DNA sequencing. Higher frequencies of mutant CCR2-64I (19.15%-28.79%) and SDF1-3’A (19.10% -29.86%) alleles were found in subjects of 8 ethnic groups in the Chinese mainland. In contrast, the △32 mutation in CCRS gene occurs at a very low frequency (0.0016, n=1287) in Han population. A relatively high frequency of CCR5-wt/△32 heterozygotes was observed in Uygurian and Mongolian populations. No A32 mutation allele was detected in Tibetan and other 4 ethnic groups in Yunnan Province. There was no CCR5-m303 mutation in subjects of any ethnic group in the Chinese展开更多
基金supported by the National Basic Research Program of China(Grant No. 2007CB815800)the National High Technology Research and Development Program of China(Grant No. 2006330004104456)the National Natural Science Foundation of China(Grant Nos. 30300264,30270693 and 30570967)
文摘Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily.In this study,we identified two novel members of the Smad family,termed as AmphiSmad1/5/8 and AmphiSmad4,from Chinese amphioxus.Both AmphiSmad1/5/8 and AmphiSmad4 showed a typical domain structure of Smad proteins consisting of conserved MH1 and MH2 domains.Phylogenetic analysis placed AmphiSmad1/5/8 in the Smad1,5 and 8 subgroup of the R-Smad subfamily,and AmphiSmad4 in the Co-Smad subfamily.The spatial and temporal gene expression patterns of AmphiSmad1/5/8 and AmphiSmad4 showed that they may be involved in the embryonic development of notochord,myotome and alimentary canal,and may help to establish the specification of dorsal-ventral axis of amphioxus.Moreover,AmphiSmad1/5/8 and AmphiSmad4 showed extensive distribution in all adult tissues examined,suggesting that these two genes may play important roles in the morphogenesis of a variety of tissues especially notochord and gonad.
文摘现代生命科学的兴起引发了学术界在思维方式及对生命本质认知上的巨大变革,随着现代科学技术的快速发展,以精准医学和系统生物学为代表的现代生物医学,在认识人类生命的范畴上,不断地与中医关于生命认识的整体观和天人合一的思维接近。二者虽属不同的学术思维体系,但随着全球人类对于生命呵护需求的趋同化和高质化,中医和西医最终走向融合是未来发展的必然趋势。为了推动这种融合更加有序和快速形成,在此试图提出一个崭新的学科概念:中医生命科学(Life Science From the Perspective of Chinese Medicine)。此概念涵盖四大要素,即物质、能量、信息、时空。通过深入挖掘中医生命观与现代科学的结合点,以期推动基于中医生命科学的新医学发展。起始的具体研究方向分别是中医生命的系统物质基础,中医生命的能量、信息、时空变化与规律,中医药大数据的智慧凝练,中医生命医学工程的设备开发。使中医在现代的环境下能够更加有效地服务于人类健康事业,实现中西医学整合框架下的融合,为新医学的建立提供生命科学的基础支撑。
文摘Limited genetic information is available concerning the polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. The aim of this study is to identify the allelic frequencies of the chemokine and chemokine receptor genes in the Chinese mainland. Genomic DNA samples extracted from whole blood of 2318 subjects were analyzed by using PCR or PCR/restriction fragment length polymorphism (RFLP) assays, and further confirmed by direct DNA sequencing. Higher frequencies of mutant CCR2-64I (19.15%-28.79%) and SDF1-3’A (19.10% -29.86%) alleles were found in subjects of 8 ethnic groups in the Chinese mainland. In contrast, the △32 mutation in CCRS gene occurs at a very low frequency (0.0016, n=1287) in Han population. A relatively high frequency of CCR5-wt/△32 heterozygotes was observed in Uygurian and Mongolian populations. No A32 mutation allele was detected in Tibetan and other 4 ethnic groups in Yunnan Province. There was no CCR5-m303 mutation in subjects of any ethnic group in the Chinese
