Using a cDNA microarray consisting of 9198 expressed sequence tags,we surveyed the gene expression profiles in shoots and roots of a rice hybrid,Liangyoupei 9 and its parents Peiai 64s and 93-11 at 72 h after germinat...Using a cDNA microarray consisting of 9198 expressed sequence tags,we surveyed the gene expression profiles in shoots and roots of a rice hybrid,Liangyoupei 9 and its parents Peiai 64s and 93-11 at 72 h after germination.A total of 8587 sequences had detectable signals in both shoots and roots of the three genotypes.A total of 1571 sequences exhibited significant(P<0.01)expression differences in shoots or roots among the three genotypes,of which 121 showed expression poly-morphisms in both shoots and roots,and 870 revealed significant expression differences between the hybrid and one of the parents.The expression polymorphism of the sequences was associated with the functional categories of the sequences.They occurred more frequently in categories of carbohy-drate,energy and lipid metabolisms and stress response than expected,while less frequently in categories of amino acid metabolism,transcription and translation regulation,and signal transduction.A total of 214 sequences exhibited significant(P<0.05)mid-parent heterosis in expression,of which 117 had homology to genes with known functions,assigned in the categories of basic metabolism,genetic information processing,cell growth and death,signal transduction,transportation and stress response.The results may provide useful information for exploring the relationship between gene expression polymorphism and phenotypic variation,and for characterizing the molecular mechanism of seedling development and heterosis in rice.展开更多
To enhance understanding of the genetic basis of trait correlation in rice,a recombinant inbred line(RIL)population(F6 and F7) from a cross between Zhenshan97 and HR5 was employed to identify main quantitative trait l...To enhance understanding of the genetic basis of trait correlation in rice,a recombinant inbred line(RIL)population(F6 and F7) from a cross between Zhenshan97 and HR5 was employed to identify main quantitative trait loci(QTLs)and epistatic QTL(E-QTL).Highly significant positive correlations were detected among five traits of heading date(HD),plant height(PH),panicle length(PL),flag leaf length(FLL)and flag leaf width(FLW)in 2 environments.Four to 8 main QTLs were detected for an individual trait.No E-QTL was detected for PH.One,4,4 and 5 E-QTLs were detected for FLL,HD,FLW and PL,respectively.Each E-QTL individually explained less than 3%of trait variation except E-QFll1.Comparison of QTL results was made in order to dissect the genetic basis of trait correlation.We found that main QTLs with pleiotropic effects and QTL clusters were the main genetic basis of trait correlation.No E-QTL had pleiotropic effects.E-QTL played an important role in the genetic basis of individual trait,but it made a little contribution to trait correlation.展开更多
基金supported by the National Program on the Development of Basic Research(Grant No.2001CB1088)the National Special Key Project of Functional Genomics and Biochips(Grant No.2002AA2Z1002)the National Natural Science Foundation of China(Grant No.30321005).
文摘Using a cDNA microarray consisting of 9198 expressed sequence tags,we surveyed the gene expression profiles in shoots and roots of a rice hybrid,Liangyoupei 9 and its parents Peiai 64s and 93-11 at 72 h after germination.A total of 8587 sequences had detectable signals in both shoots and roots of the three genotypes.A total of 1571 sequences exhibited significant(P<0.01)expression differences in shoots or roots among the three genotypes,of which 121 showed expression poly-morphisms in both shoots and roots,and 870 revealed significant expression differences between the hybrid and one of the parents.The expression polymorphism of the sequences was associated with the functional categories of the sequences.They occurred more frequently in categories of carbohy-drate,energy and lipid metabolisms and stress response than expected,while less frequently in categories of amino acid metabolism,transcription and translation regulation,and signal transduction.A total of 214 sequences exhibited significant(P<0.05)mid-parent heterosis in expression,of which 117 had homology to genes with known functions,assigned in the categories of basic metabolism,genetic information processing,cell growth and death,signal transduction,transportation and stress response.The results may provide useful information for exploring the relationship between gene expression polymorphism and phenotypic variation,and for characterizing the molecular mechanism of seedling development and heterosis in rice.
基金supported by grants from the National Basic Research Program of China(2007CB109001)the Doctoral Fund for Promoting Scientific Research from the Natural Science Foundation of Guangdong Province(8452840301001691)
文摘To enhance understanding of the genetic basis of trait correlation in rice,a recombinant inbred line(RIL)population(F6 and F7) from a cross between Zhenshan97 and HR5 was employed to identify main quantitative trait loci(QTLs)and epistatic QTL(E-QTL).Highly significant positive correlations were detected among five traits of heading date(HD),plant height(PH),panicle length(PL),flag leaf length(FLL)and flag leaf width(FLW)in 2 environments.Four to 8 main QTLs were detected for an individual trait.No E-QTL was detected for PH.One,4,4 and 5 E-QTLs were detected for FLL,HD,FLW and PL,respectively.Each E-QTL individually explained less than 3%of trait variation except E-QFll1.Comparison of QTL results was made in order to dissect the genetic basis of trait correlation.We found that main QTLs with pleiotropic effects and QTL clusters were the main genetic basis of trait correlation.No E-QTL had pleiotropic effects.E-QTL played an important role in the genetic basis of individual trait,but it made a little contribution to trait correlation.
