With the global spread of antimicrobial resistance,it is imperative to satisfy the need to develop novel antibacterial agents.Here,we synthesized chiral Co_(3)O_(4) nanoparticles (NPs) with obvious bactericidal effect...With the global spread of antimicrobial resistance,it is imperative to satisfy the need to develop novel antibacterial agents.Here,we synthesized chiral Co_(3)O_(4) nanoparticles (NPs) with obvious bactericidal effects.It was found that the circular dichroism (CD) spectra of L-Co_(3)O_(4) NPs and D-Co_(3)O_(4) NPs exhibited perfect mirror image inversion.Specifically,the survival rate of primary uterine fibroblast (PCS) cells reached above 90% by co-culturing with chiral Co_(3)O_(4) NPs.Besides,the chiral Co_(3)O_(4) NPs showed obvious killing ability on Gram-positive (Staphylococcus aureus);Gram-negative (Escherichia coli O157:H7) bacteria both in vitro and in vivo.Particularly,D-Co_(3)O_(4) NPs showed better antibacterial activity.Mechanistic studies confirmed that the level of reactive oxygen species level generated by D-Co_(3)O_(4) NPs was higher than that of L-Co_(3)O_(4) NPs.Overall,the synthesized D-Co_(3)O_(4) NPs have the potential to become a kind of novel antibacterial nanomaterials.展开更多
基金supported by the National Key R&D Program of China(No.2022YFF1102600).
文摘With the global spread of antimicrobial resistance,it is imperative to satisfy the need to develop novel antibacterial agents.Here,we synthesized chiral Co_(3)O_(4) nanoparticles (NPs) with obvious bactericidal effects.It was found that the circular dichroism (CD) spectra of L-Co_(3)O_(4) NPs and D-Co_(3)O_(4) NPs exhibited perfect mirror image inversion.Specifically,the survival rate of primary uterine fibroblast (PCS) cells reached above 90% by co-culturing with chiral Co_(3)O_(4) NPs.Besides,the chiral Co_(3)O_(4) NPs showed obvious killing ability on Gram-positive (Staphylococcus aureus);Gram-negative (Escherichia coli O157:H7) bacteria both in vitro and in vivo.Particularly,D-Co_(3)O_(4) NPs showed better antibacterial activity.Mechanistic studies confirmed that the level of reactive oxygen species level generated by D-Co_(3)O_(4) NPs was higher than that of L-Co_(3)O_(4) NPs.Overall,the synthesized D-Co_(3)O_(4) NPs have the potential to become a kind of novel antibacterial nanomaterials.
