目的评价核桃蛋白水解肽(walnut protein hydrolysates,WPH)对酒精性认知下降的改善效应并探讨其作用机制。方法40只雄性SD大鼠随机分为空白对照组(Control)、酒精模型组(Model)、WPH干预低、中、高剂量组(WPH-L、WPH-M、WPH-H)。Morri...目的评价核桃蛋白水解肽(walnut protein hydrolysates,WPH)对酒精性认知下降的改善效应并探讨其作用机制。方法40只雄性SD大鼠随机分为空白对照组(Control)、酒精模型组(Model)、WPH干预低、中、高剂量组(WPH-L、WPH-M、WPH-H)。Morris水迷宫检测空间学习记忆能力,分析免疫相关血细胞指标,组织病理学分析海马区神经元细胞密度,超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)分析大脑皮质内记忆相关神经递质含量,蛋白质印迹法(Western blotting)和逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测突触信号传递标记蛋白及其基因表达水平。结果与Control相比,Model组平台潜伏期、运动总路程显著延长,其目标象限平台穿越次数与停留时间显著下降(P<0.05);WPH干预后平台潜伏期、运动总路程均剂量依赖性降低,目标象限平台穿越次数、停留时间均剂量依赖性增加,且在WPH-H组达到显著性水平(P<0.05),表明WPH干预后能有效缓解长期酒精摄入导致的认知下降;长期酒精摄入诱导炎性细胞(白细胞和淋巴细胞)水平显著升高(P<0.05),海马CA1区神经元细胞明显减少,细胞核出现固缩;高剂量WPH干预后炎性细胞水平显著降低(P<0.05),神经元数目明显上升,细胞排列较为整齐,表明WPH可有效减轻长期酒精摄入诱导的海马区神经元丢失;此外,高剂量WPH可明显改善长期酒精摄入引起的大脑皮质神经递质紊乱(乙酰胆碱、多巴胺、5-羟色胺和γ-氨基丁酸),且可显著逆转神经细胞突触信号传递关键蛋白分子BDNF、CREB、PSD95和ERK1/2及其基因表达水平的下降(P<0.05)。结论本研究初步揭示WPH改善酒精性认知下降的海马结构基础和突触分子机制,为核桃蛋白的高值化利用奠定了理论基础。展开更多
Two new lignan glucosides,tinsinlignans A and B(1 and 2),two new oxyneolignans,tinsinlignans C and D(3 and 4),along with one known analogue(5),were isolated from the stems of Tinospora sinensis.The structures of the n...Two new lignan glucosides,tinsinlignans A and B(1 and 2),two new oxyneolignans,tinsinlignans C and D(3 and 4),along with one known analogue(5),were isolated from the stems of Tinospora sinensis.The structures of the new compounds were elucidated based on analysis of spectroscopic data,and the absolute configuration of 1 was determined through electronic circular dichroism(ECD)calculation based on the time-dependent density functional theory(TD-DFT).Compounds 1−4 were evaluated for their inhibitory effects on nitric oxide(NO)production induced by lipopolysaccharide(LPS)in murine RAW264.7 macrophage cells and compounds 1 and 2 exhibited moderate inhibitory activities with IC50 values of 18.5±2.0 and 28.8±1.2μmol·L^(−1),respectively.展开更多
基金supported by the Natural Science Foundation of Shandong Province(No.JQ201721)the Young Taishan Scholars Program(No.tsqn20161037)+1 种基金the Scientific Research Starting Fund from University of Jinan(No.1009472)the Innovation Team Project of Jinan Science&Technology Bureau(No.2018GXRC003).
文摘Two new lignan glucosides,tinsinlignans A and B(1 and 2),two new oxyneolignans,tinsinlignans C and D(3 and 4),along with one known analogue(5),were isolated from the stems of Tinospora sinensis.The structures of the new compounds were elucidated based on analysis of spectroscopic data,and the absolute configuration of 1 was determined through electronic circular dichroism(ECD)calculation based on the time-dependent density functional theory(TD-DFT).Compounds 1−4 were evaluated for their inhibitory effects on nitric oxide(NO)production induced by lipopolysaccharide(LPS)in murine RAW264.7 macrophage cells and compounds 1 and 2 exhibited moderate inhibitory activities with IC50 values of 18.5±2.0 and 28.8±1.2μmol·L^(−1),respectively.
