区块链视角下新能源汽车电池逆向物流网络构建及优化

新能源汽车产业步入飞速发展的新时期,但其汽车动力电池的巨大消耗给我国新能源汽车电池逆向物流带来了新的挑战。文中根据区块链技术特点,采用系统动力学的方法,构建区块链视角下的新能源汽车电池逆向物流网络模型,分析新能源汽车电池逆向物流网络的特点,并对该模型进行了仿真,根据仿真的结果,提出了相对应的政策建议。

Development of SNP markers using RNA-seq technology and tetraprimer ARMS-PCR in sweetpotato

The information of single nucleotide polymorphisms （SNPs） is quite unknown in sweetpotato. In this study, two sweetpotato varieties （Xushu 18 and Xu 781） were sequenced by Illumina technology, as well as de novo transcriptome assembly, functional annotation, and in silico discovery of potential SNP molecular markers. Tetra-primer Amplification Refractory Mutation System PCR （ARMS-PCR） is a simple and sufficient method for detecting different alleles in SNP locus. Total 153 sets of ARMS-PCR primers were designed to validate the putative SNPs from sequences. PCR products from 103 sets of primers were different between Xu 781 and Xushu 18 via agarose gel electrophoresis, and the detection rate was 67.32%. We obtained the expected results from 32 sets of primers between the two genotypes. Furthermore, we ascertained the optimal annealing temperature of 32 sets of primers. These SNPs might be used in genotyping, QTL mapping, or marker-assisted trait selection further in sweetpotato. To our knowledge, this work was the first study to develop SNP markers in sweetpotato by using tetra-primer ARMS-PCR technique. This method was a simple, rapid, and useful techn!que to develop SNP markers, and will provide a potential and preliminary application in discriminating cultivars in sweetpotato.

滚珠丝杠副表面质量对噪声性能影响的试验研究

针对滚珠丝杠副噪声测量试验给出了测量系统的组成和原理,在此基础上针对不同表面粗糙度和不同转速下的KD4032端塞式滚珠丝杠副进行了噪声测量试验。试验结果分析表明,滚道表面波纹度的激励频率计算值与噪声测量所得的主频基本一致且波纹度为噪声的主要来源,可以通过提高表面加工质量达到降低噪声、改善国产滚珠丝杠副高速性能的目的。

三产融合发展 助推乡村振兴——以湖南新化县油溪桥村为例

在对湖南省新化县油溪桥村五次深入跟踪采访、调研、考察、讲学的基础上,研究该村三产融合发展实践。研究结果表明:三产的融合方式及配套的利益联结机制极大地促进了该村的经济发展;同时也指出该村存在三产业融合深度不够、优质技能人才严重不足、商标和品牌意识淡薄、资金短缺、示范引领作用不很明显等问题;最后从推进农旅产品转型升级、培育人才、引进技术、组建乡贤参事会、加速品牌建设、弘扬梅山文化、改善村容风貌等方面提出了八点建议。