采用代谢组学方法探究炙淫羊藿温肾助阳和炮制增效机制。建立氢化可的松诱导的大鼠肾阳虚证模型,基于UPLC-Q-TOF-MS(ultra-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry)代谢组学方法,...采用代谢组学方法探究炙淫羊藿温肾助阳和炮制增效机制。建立氢化可的松诱导的大鼠肾阳虚证模型,基于UPLC-Q-TOF-MS(ultra-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry)代谢组学方法,联合多元统计分析和单变量统计分析,筛选并鉴定血浆、尿液样品中与肾阳虚相关的潜在生物标志物,分析羊脂油组、淫羊藿生品组、淫羊藿加热品组、淫羊藿炙品组改善肾阳虚证的代谢调控机制。结果显示,氢化可的松诱导的肾阳虚大鼠血浆、尿液代谢呈现明显的轨迹变化,在血浆和尿液中鉴定出15种与肾阳虚相关的生物标志物,涉及5条代谢通路,分别为甘油磷脂代谢、鞘脂代谢、硫代谢、乙醛酸和二羧酸代谢、半胱氨酸和蛋氨酸代谢。炙淫羊藿温肾助阳的代谢通路涉及甘油磷脂代谢、半胱氨酸和蛋氨酸代谢,而炙淫羊藿两个炮制因素“加热”“羊脂油”分别通过调节甘油磷脂代谢、半胱氨酸和蛋氨酸代谢来增强其温肾助阳的作用,进而阐明了炙淫羊藿的炮制增效机制。本文涉及的动物实验符合伦理学标准,并且已获得江苏省中医药研究院动物伦理委员会批准(批准号:AEWC-20200702-119)。展开更多
Triptolide(TP) from Tripterygium wilfordii has been demonstrated to possess anti-inflammatory, immunosuppressive, and anticancer activities. TP is specially used for the treatment of awkward rheumatoid arthritis, but ...Triptolide(TP) from Tripterygium wilfordii has been demonstrated to possess anti-inflammatory, immunosuppressive, and anticancer activities. TP is specially used for the treatment of awkward rheumatoid arthritis, but its clinical application is confined by intense side effects. It is reported that licorice can obviously reduce the toxicity of TP, but the detailed mechanisms involved have not been comprehensively investigated. The current study aimed to explore metabolomics characteristics of the toxic reaction induced by TP and the intervention effect of licorice water extraction(LWE) against such toxicity. Obtained urine samples from control, TP and TP + LWE treated rats were analyzed by UPLC/ESI-QTOF-MS. The metabolic profiles of the control and the TP group were well differentiated by the principal component analysis and orthogonal partial least squares-discriminant analysis. The toxicity of TP was demonstrated to be evolving along with the exposure time of TP. Eight potential biomarkers related to TP toxicity were successfully identified in urine samples. Furthermore, LWE treatment could attenuate the change in six of the eight identified biomarkers. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan, pantothenic acid, and porphyrin metabolism. Therefore, it was concluded that LWE demonstrated interventional effects on TP toxicity through regulation of tryptophan, pantothenic acid, and porphyrin metabolism pathways, which provided novel insights into the possible mechanisms of TP toxicity as well as the potential therapeutic effects of LWE against such toxicity.展开更多
Boiling processing is commonly used in post-harvest handling of White Paeony Root(WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a high...Boiling processing is commonly used in post-harvest handling of White Paeony Root(WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 min could both make the WPR bright-colored and improve the contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling(2-10 min) is recommended for post-harvest handling of WPR.展开更多
文摘采用代谢组学方法探究炙淫羊藿温肾助阳和炮制增效机制。建立氢化可的松诱导的大鼠肾阳虚证模型,基于UPLC-Q-TOF-MS(ultra-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry)代谢组学方法,联合多元统计分析和单变量统计分析,筛选并鉴定血浆、尿液样品中与肾阳虚相关的潜在生物标志物,分析羊脂油组、淫羊藿生品组、淫羊藿加热品组、淫羊藿炙品组改善肾阳虚证的代谢调控机制。结果显示,氢化可的松诱导的肾阳虚大鼠血浆、尿液代谢呈现明显的轨迹变化,在血浆和尿液中鉴定出15种与肾阳虚相关的生物标志物,涉及5条代谢通路,分别为甘油磷脂代谢、鞘脂代谢、硫代谢、乙醛酸和二羧酸代谢、半胱氨酸和蛋氨酸代谢。炙淫羊藿温肾助阳的代谢通路涉及甘油磷脂代谢、半胱氨酸和蛋氨酸代谢,而炙淫羊藿两个炮制因素“加热”“羊脂油”分别通过调节甘油磷脂代谢、半胱氨酸和蛋氨酸代谢来增强其温肾助阳的作用,进而阐明了炙淫羊藿的炮制增效机制。本文涉及的动物实验符合伦理学标准,并且已获得江苏省中医药研究院动物伦理委员会批准(批准号:AEWC-20200702-119)。
基金supported by the National Natural Science Foundation of China(Grant Nos.81160541and 81373946)the Project of Health Department of Jiangxi Province(Grant No.2011A143)the priority academic program development of Jiangsu higher education institutions(PAPD)
文摘Triptolide(TP) from Tripterygium wilfordii has been demonstrated to possess anti-inflammatory, immunosuppressive, and anticancer activities. TP is specially used for the treatment of awkward rheumatoid arthritis, but its clinical application is confined by intense side effects. It is reported that licorice can obviously reduce the toxicity of TP, but the detailed mechanisms involved have not been comprehensively investigated. The current study aimed to explore metabolomics characteristics of the toxic reaction induced by TP and the intervention effect of licorice water extraction(LWE) against such toxicity. Obtained urine samples from control, TP and TP + LWE treated rats were analyzed by UPLC/ESI-QTOF-MS. The metabolic profiles of the control and the TP group were well differentiated by the principal component analysis and orthogonal partial least squares-discriminant analysis. The toxicity of TP was demonstrated to be evolving along with the exposure time of TP. Eight potential biomarkers related to TP toxicity were successfully identified in urine samples. Furthermore, LWE treatment could attenuate the change in six of the eight identified biomarkers. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan, pantothenic acid, and porphyrin metabolism. Therefore, it was concluded that LWE demonstrated interventional effects on TP toxicity through regulation of tryptophan, pantothenic acid, and porphyrin metabolism pathways, which provided novel insights into the possible mechanisms of TP toxicity as well as the potential therapeutic effects of LWE against such toxicity.
基金supported by a special fund of China State Administration of Traditional Chinese Medicine(No.201307008-2)National High Technology Research and Development Plan of China(863 Plain)(No.2014AA022204)+1 种基金Jiangsu Province Six Talent Project(YY-007)Jiangsu Branch of China Academy of Chinese Medical Science(No.JSBN1301)
文摘Boiling processing is commonly used in post-harvest handling of White Paeony Root(WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 min could both make the WPR bright-colored and improve the contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling(2-10 min) is recommended for post-harvest handling of WPR.
