Objectives:To investigate the effect on essential hypertension of the topical application of TAT-Cu,Zn-superoxide dismutase(TAT-SOD)at left acupoint Zusanli(ST 36),and to observe whether the change of electrical poten...Objectives:To investigate the effect on essential hypertension of the topical application of TAT-Cu,Zn-superoxide dismutase(TAT-SOD)at left acupoint Zusanli(ST 36),and to observe whether the change of electrical potential difference(EPD)can be related to the change of blood pressure.Methods:Sixteen patients with essential hypertension and 16 healthy subjects were included in the study.EPD between the left acupoints of Yanglingquan(GB 34)and Qiuxu(GB 40)was firstly screened out for the EPD detection.An intracellular superoxide quenching enzyme,TAT-SOD,was topically applied to the acupoint ST 36 within an area of 1 cm^2 once a day,and the influence on EPD was investigated.The dosage applied to TAT-SOD group(n=8)was 0.2 mL of 3000 U/mL TAT-SOD cream prepared by adding purified TAT-SOD to a vehicle cream,while placebo group(n=8)used the vehicle cream instead.The left acupoints of Yanglingquan(GB 34)and Qiuxu(GB 40)were selected for EPD measurement after comparing EPD readings between 5 acupoints on each of all 12 meridians.Results:EPDs between the left acupoints of GB 34 and GB 40 for 16 patients of essential hypertension and 16 healthy subjects were 44.9±6.4 and 5.6±0.9 mV,respectively.Daily application of TAT-SOD for 15 days at ST 36 of essential hypertension patients significantly decreased systolic blood pressure(SBP)and diastolic blood pressure(DBP)of 179.6 and 81.5 mm Hg to 153.1 and 74.1 mm Hg,respectively.Responding to the change in blood pressure,EPD between the left acupoints of GB 34 and GB 40 also declined from 44.4 to 22.8 mV with the same trend.No change was observed with SBP,DBP and EPD between the left acupoints of GB 34 and GB 40 with the daily application of the placebo cream.Conclusion:Enzymatic scavenging of the intracellular superoxide at ST 36 proved to be effective in decreasing SBP and DBP.The results reconfirm the involvement of superoxide anions and its transportation along the meridians,and demonstrate that EPD between acupoints may be an indicator to reflect its functioning status.Moreover,prellmlnary results suggest a close correlatlon between EPD and blood pressure readings,implying a possibility of using EPD as a sensitive parameter for blood pressure and to monitor the effect of antihypertensive treatment.展开更多
Objective:To evaluate the effect of the pulse width of electroacupuncture(EA)in the treatment of denervation-induced skeletal muscle atrophy in rats and examine the role of insulin-like growth factor 1(IGF-1)/phosphat...Objective:To evaluate the effect of the pulse width of electroacupuncture(EA)in the treatment of denervation-induced skeletal muscle atrophy in rats and examine the role of insulin-like growth factor 1(IGF-1)/phosphatidylinositol 3-kinase(PI3 K)/Akt signaling pathway during EA.Methods:Sciatic nerve functional index(SFI),muscle wet weight and the cross-sectional area(CSA)of the gastrocnemius muscle were analyzed after treatment in model rats with EA of various pulse widths(0.5,50,100 and 200 ms).The apoptosis index(AI)and paired box(PAX)3 and PAX7 protein expression were also determined.Further,the mRNA and protein expressions of components of IGF-1/PI3 K/Akt pathway and their downstream targets were determined,along with the inhibiting effect of the pathway with a PI3-specific inhibitor.Results:EA with a pulse width of 200 ms was found to have the best effect with regard to increasing SFI,CSA and muscle weight,decreasing AI,and increasing the expression of PAX3 and PAX7.The IGF-1/PI3 K/Akt pathway was found to be activated by denervation,although the downstream forkhead box O(FoxO)pathway was not suppressed by its activation.The PI3 K/Akt pathway and its downstream molecule mammalian target of rapamycin(mTOR)were up-regulated further by EA to promote muscle protein synthesis.Meanwhile,the expressions of downstream FoxO and F-box protein 32(ATROGIN-1)were down-regulated to reduce protein degradation.Conclusions:EA with 200-ms pulse width was found to have a more significant effect than 0.5-ms EA.The positive effects of EA disappeared after inhibition of the PI3 K/Akt pathway.展开更多
基金National Key R&D Program of China(No.2016YFD0400202)National Natural Science Foundation of China(No.31500685,30973720)+1 种基金Zhejiang Provincial Natural Science Foundation of China(No.LY16C050002)Start-Up Fund of Research in Quanzhou Normal University(No.G16062)。
文摘Objectives:To investigate the effect on essential hypertension of the topical application of TAT-Cu,Zn-superoxide dismutase(TAT-SOD)at left acupoint Zusanli(ST 36),and to observe whether the change of electrical potential difference(EPD)can be related to the change of blood pressure.Methods:Sixteen patients with essential hypertension and 16 healthy subjects were included in the study.EPD between the left acupoints of Yanglingquan(GB 34)and Qiuxu(GB 40)was firstly screened out for the EPD detection.An intracellular superoxide quenching enzyme,TAT-SOD,was topically applied to the acupoint ST 36 within an area of 1 cm^2 once a day,and the influence on EPD was investigated.The dosage applied to TAT-SOD group(n=8)was 0.2 mL of 3000 U/mL TAT-SOD cream prepared by adding purified TAT-SOD to a vehicle cream,while placebo group(n=8)used the vehicle cream instead.The left acupoints of Yanglingquan(GB 34)and Qiuxu(GB 40)were selected for EPD measurement after comparing EPD readings between 5 acupoints on each of all 12 meridians.Results:EPDs between the left acupoints of GB 34 and GB 40 for 16 patients of essential hypertension and 16 healthy subjects were 44.9±6.4 and 5.6±0.9 mV,respectively.Daily application of TAT-SOD for 15 days at ST 36 of essential hypertension patients significantly decreased systolic blood pressure(SBP)and diastolic blood pressure(DBP)of 179.6 and 81.5 mm Hg to 153.1 and 74.1 mm Hg,respectively.Responding to the change in blood pressure,EPD between the left acupoints of GB 34 and GB 40 also declined from 44.4 to 22.8 mV with the same trend.No change was observed with SBP,DBP and EPD between the left acupoints of GB 34 and GB 40 with the daily application of the placebo cream.Conclusion:Enzymatic scavenging of the intracellular superoxide at ST 36 proved to be effective in decreasing SBP and DBP.The results reconfirm the involvement of superoxide anions and its transportation along the meridians,and demonstrate that EPD between acupoints may be an indicator to reflect its functioning status.Moreover,prellmlnary results suggest a close correlatlon between EPD and blood pressure readings,implying a possibility of using EPD as a sensitive parameter for blood pressure and to monitor the effect of antihypertensive treatment.
基金Supported by the National Natural Science Foundation of China(No.81503657,No.81373733)the Basic Research Project of Public Welfare Research Institutes in Fujian Province(No.2016R1033-6)。
文摘Objective:To evaluate the effect of the pulse width of electroacupuncture(EA)in the treatment of denervation-induced skeletal muscle atrophy in rats and examine the role of insulin-like growth factor 1(IGF-1)/phosphatidylinositol 3-kinase(PI3 K)/Akt signaling pathway during EA.Methods:Sciatic nerve functional index(SFI),muscle wet weight and the cross-sectional area(CSA)of the gastrocnemius muscle were analyzed after treatment in model rats with EA of various pulse widths(0.5,50,100 and 200 ms).The apoptosis index(AI)and paired box(PAX)3 and PAX7 protein expression were also determined.Further,the mRNA and protein expressions of components of IGF-1/PI3 K/Akt pathway and their downstream targets were determined,along with the inhibiting effect of the pathway with a PI3-specific inhibitor.Results:EA with a pulse width of 200 ms was found to have the best effect with regard to increasing SFI,CSA and muscle weight,decreasing AI,and increasing the expression of PAX3 and PAX7.The IGF-1/PI3 K/Akt pathway was found to be activated by denervation,although the downstream forkhead box O(FoxO)pathway was not suppressed by its activation.The PI3 K/Akt pathway and its downstream molecule mammalian target of rapamycin(mTOR)were up-regulated further by EA to promote muscle protein synthesis.Meanwhile,the expressions of downstream FoxO and F-box protein 32(ATROGIN-1)were down-regulated to reduce protein degradation.Conclusions:EA with 200-ms pulse width was found to have a more significant effect than 0.5-ms EA.The positive effects of EA disappeared after inhibition of the PI3 K/Akt pathway.
