Background Iron is a biocorrodible metal that might be used in bioabsorbable stents.This study investigated the effects at the cellular and protein levels of soluble divalent iron （ferrous gluconate） and soluble tri...Background Iron is a biocorrodible metal that might be used in bioabsorbable stents.This study investigated the effects at the cellular and protein levels of soluble divalent iron （ferrous gluconate） and soluble trivalent iron （ferric chloride） on the proliferation of human aortic smooth muscle cell （HASMC） in vitro.Methods The water-soluble tetrazolium （WST-1） test was used to evaluate the effect of iron on proliferation of HASMC and Western blotting was used to measure the levels of signaling proteins involved in proliferative and apoptosis pathways.Results HASMC proliferation was inhibited in a concentration dependent manner after treatment with soluble divalent and trivalent iron at concentrations of 100-500 μmol/L.Western blotting analysis showed that the proliferating cell nuclear antigen （PCNA） expression following treatment with soluble divalent iron and trivalent iron at 100,300 and 500 μmol/L was reduced compared to the control.The PCNA expression decreased with increasing iron concentration and to a greater extent with the trivalent iron than with the divalent iron treatment group.The p53 expression was markedly increased in a concentration dependent manner in both iron treatment groups.Conclusion The soluble divalent iron and,to a greater degree trivalent iron,inhibited HASMC proliferation in a dosedependent manner,which may be attributed to reduction of PCNA expression and increase of p53 expression.展开更多
Background Dicycloplatin is a relatively safe third generation focused on the effects of dicycloplatin on in vitro proliferation （HASMC） and human aortic endothelial cells （HAEC）. platinum-complex anti-cancer drug...Background Dicycloplatin is a relatively safe third generation focused on the effects of dicycloplatin on in vitro proliferation （HASMC） and human aortic endothelial cells （HAEC）. platinum-complex anti-cancer drug. The present study and apoptosis of human aortic smooth muscle cells Methods Proliferation of HASMC and HAEC, DNA content, and cellular levels of proliferation- and apoptosis-related proteins were assessed using the （3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）- 2H-tetrazolium） （MTS） assay, flow cytometry and Western blotting assays, respectively. Results Dicycloplatin at 10 ng/ml significantly inhibited HASMC proliferation, however, 10 μg/ml were required to significantly inhibit HAEC proliferation. Cell cycle analysis showed that dicycloplatin was a non-specific inhibitor of the cell cycle. Although dicycloplatin significantly decreased proliferating cell nuclear antigen （PCNA） expression in HASMC at all concentrations tested, it did not significantly affect PCNA expression in HAEC; Bax and p53 protein expression was upregulated in dicycloplatin groups. Conclusions Dicycloplatin at nanogram concentrations significantly inhibits HASMC proliferation, although the effect is relatively weaker than that of sirolimus. In contrast, the effect of dicycloplatin on inhibition of HAEC proliferation is much less pronounced than that on HASMC. The latter characteristics point to the potential for use of dicycloplatin in drug-eluting stents.展开更多
文摘目的血小板功能检测指导心脏外科手术治疗的价值,目前仍存在争议,相关研究的中国患者的数据较少。本文旨在探讨血栓弹力图(TEG)对CABG术后输血的预测价值。方法入选阜外医院61例行CABG的患者,所有患者术前24 h内行TEG检测,临床主要终点为术后输血。结果CABG术后输血共14人(23.0%)。输血组患者TEG检测的平均二磷酸腺苷诱导的血凝块最大强度[MA(ADP)]值显著低于未输血组(28.67±18.04 vs 37.21±12.64,P=0.023)。选取MA(ADP)值31mm为划分点,≤31mm组患者术后输血风险明显较>31mm组增高(37.5%vs 13.5%,P=0.030)。多因素回归分析显示MA(ADP)≤31mm为CABG术后输血的独立预测因素,MA(ADP)≤31mm患者术后输血的风险是MA(ADP)>31mm患者的8.352倍(95%CI:1.670-41.780,P=0.010)。结论TEG血小板功能检测对CABG术后出血有一定的预测价值;MA(ADP)≤31mm为CABG术后输血的独立危险因素。
文摘Background Iron is a biocorrodible metal that might be used in bioabsorbable stents.This study investigated the effects at the cellular and protein levels of soluble divalent iron （ferrous gluconate） and soluble trivalent iron （ferric chloride） on the proliferation of human aortic smooth muscle cell （HASMC） in vitro.Methods The water-soluble tetrazolium （WST-1） test was used to evaluate the effect of iron on proliferation of HASMC and Western blotting was used to measure the levels of signaling proteins involved in proliferative and apoptosis pathways.Results HASMC proliferation was inhibited in a concentration dependent manner after treatment with soluble divalent and trivalent iron at concentrations of 100-500 μmol/L.Western blotting analysis showed that the proliferating cell nuclear antigen （PCNA） expression following treatment with soluble divalent iron and trivalent iron at 100,300 and 500 μmol/L was reduced compared to the control.The PCNA expression decreased with increasing iron concentration and to a greater extent with the trivalent iron than with the divalent iron treatment group.The p53 expression was markedly increased in a concentration dependent manner in both iron treatment groups.Conclusion The soluble divalent iron and,to a greater degree trivalent iron,inhibited HASMC proliferation in a dosedependent manner,which may be attributed to reduction of PCNA expression and increase of p53 expression.
文摘Background Dicycloplatin is a relatively safe third generation focused on the effects of dicycloplatin on in vitro proliferation （HASMC） and human aortic endothelial cells （HAEC）. platinum-complex anti-cancer drug. The present study and apoptosis of human aortic smooth muscle cells Methods Proliferation of HASMC and HAEC, DNA content, and cellular levels of proliferation- and apoptosis-related proteins were assessed using the （3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）- 2H-tetrazolium） （MTS） assay, flow cytometry and Western blotting assays, respectively. Results Dicycloplatin at 10 ng/ml significantly inhibited HASMC proliferation, however, 10 μg/ml were required to significantly inhibit HAEC proliferation. Cell cycle analysis showed that dicycloplatin was a non-specific inhibitor of the cell cycle. Although dicycloplatin significantly decreased proliferating cell nuclear antigen （PCNA） expression in HASMC at all concentrations tested, it did not significantly affect PCNA expression in HAEC; Bax and p53 protein expression was upregulated in dicycloplatin groups. Conclusions Dicycloplatin at nanogram concentrations significantly inhibits HASMC proliferation, although the effect is relatively weaker than that of sirolimus. In contrast, the effect of dicycloplatin on inhibition of HAEC proliferation is much less pronounced than that on HASMC. The latter characteristics point to the potential for use of dicycloplatin in drug-eluting stents.
