目的:探讨吲哚菁绿荧光成像技术在完全腹腔镜结肠癌手术中的应用价值。方法:回顾分析2018年1月至2020年2月行完全腹腔镜手术并经病理确诊的结肠癌患者的临床资料,其中研究组37例(吲哚菁绿荧光成像),对照组39例(传统腹腔镜或3D腹腔镜手...目的:探讨吲哚菁绿荧光成像技术在完全腹腔镜结肠癌手术中的应用价值。方法:回顾分析2018年1月至2020年2月行完全腹腔镜手术并经病理确诊的结肠癌患者的临床资料,其中研究组37例(吲哚菁绿荧光成像),对照组39例(传统腹腔镜或3D腹腔镜手术),对比分析两组患者病理特征、手术及术后并发症情况。结果:两组患者年龄、性别、BMI、ASA分级、肿瘤部位、术前合并症差异均无统计学意义(P>0.05)。研究组中位手术时间短于对照组(184 min vs.200 min),差异有统计学意义(P<0.05);淋巴结清扫数量多于对照组[(27.5±4.1)vs.(23.7±5.0)],差异有统计学意义(P<0.05)。两组均未出现吻合口狭窄、吻合口漏等严重并发症,两组手术并发症、术后住院时间差异无统计学意义。结论:吲哚菁绿荧光成像技术在完全腹腔镜结肠癌手术中是安全、可行的,有助于目标肠段的定位及吻合口血供的快速判断,可缩短手术时间,减少吻合口漏的发生。展开更多
Soybean is a global principal source of edible plant oil. As more soybean oil-related quantitative trait loci(QTLs) have been located in the collective genome, it is urgent to establish a classification system for the...Soybean is a global principal source of edible plant oil. As more soybean oil-related quantitative trait loci(QTLs) have been located in the collective genome, it is urgent to establish a classification system for these distributed QTLs. A collinear platform may be useful to characterize and identify relationships among QTLs as well as aid in novel gene discovery. In this study, the collinearity MCScan X algorithm and collective soybean genomic information were used to construct collinearity blocks, to which soybean oil-related QTLs were mapped. The results demonstrated that 666 collinearity blocks were detected in the soybean genome across 20 chromosomes, and 521 collinearity relationships existed in 231 of the 242 effective soybean oil-related QTLs. This included 214 inclusion relationships and 307 intersecting relationships. Among them, the collinearity among QTLs that are related to soybean oil content was shown on a maximum of seven chromosomes and minimum of one chromosome, with the majority of QTLs having collinearity on two chromosomes. Using overlapping hotspot regions in the soybean oil QTLs with collinearity, we mined for novel oil content-related genes. Overall, we identified 23 putatively functional genes associated with oil content in soybean and annotated them using a number of annotation databases. Our findings provide a valuable framework for elucidating evolutionary relationships between soybean oil-related QTLs and lay a foundation for functional marker-assisted breeding relating to soybean oil content.展开更多
文摘目的:探讨吲哚菁绿荧光成像技术在完全腹腔镜结肠癌手术中的应用价值。方法:回顾分析2018年1月至2020年2月行完全腹腔镜手术并经病理确诊的结肠癌患者的临床资料,其中研究组37例(吲哚菁绿荧光成像),对照组39例(传统腹腔镜或3D腹腔镜手术),对比分析两组患者病理特征、手术及术后并发症情况。结果:两组患者年龄、性别、BMI、ASA分级、肿瘤部位、术前合并症差异均无统计学意义(P>0.05)。研究组中位手术时间短于对照组(184 min vs.200 min),差异有统计学意义(P<0.05);淋巴结清扫数量多于对照组[(27.5±4.1)vs.(23.7±5.0)],差异有统计学意义(P<0.05)。两组均未出现吻合口狭窄、吻合口漏等严重并发症,两组手术并发症、术后住院时间差异无统计学意义。结论:吲哚菁绿荧光成像技术在完全腹腔镜结肠癌手术中是安全、可行的,有助于目标肠段的定位及吻合口血供的快速判断,可缩短手术时间,减少吻合口漏的发生。
基金financially supported by the National Key R&D Program of China (2016YFD0100500, 2016YFD0100300, 2016YFD0100201-21)the National Natural Science Foundation of China (31701449, 31471516, 31401465, 31400074, 31501332)+7 种基金the Natural Science Foundation of Heilongjiang Province, China (QC2017013)the Young Innovative Talent Training Plan of Undergraduate Colleges and Universities in Heilongjiang Province, China (UNPYSCT-2016144)the Special Financial Aid to Postdoctor Research Fellow in Heilongjiang, China (To Qi Zhaoming)the Heilongjiang Funds for Distinguished Young Scientists, China (JC2016004)the Outstanding Academic Leaders Projects of Harbin, China (2015RQXXJ018)the China Post Doctoral Project (2015M581419)the Dongnongxuezhe Project, China (to Chen Qingshan)the Young Talent Project of Northeast Agricultural University, China (to Qi Zhaoming, 518062)
文摘Soybean is a global principal source of edible plant oil. As more soybean oil-related quantitative trait loci(QTLs) have been located in the collective genome, it is urgent to establish a classification system for these distributed QTLs. A collinear platform may be useful to characterize and identify relationships among QTLs as well as aid in novel gene discovery. In this study, the collinearity MCScan X algorithm and collective soybean genomic information were used to construct collinearity blocks, to which soybean oil-related QTLs were mapped. The results demonstrated that 666 collinearity blocks were detected in the soybean genome across 20 chromosomes, and 521 collinearity relationships existed in 231 of the 242 effective soybean oil-related QTLs. This included 214 inclusion relationships and 307 intersecting relationships. Among them, the collinearity among QTLs that are related to soybean oil content was shown on a maximum of seven chromosomes and minimum of one chromosome, with the majority of QTLs having collinearity on two chromosomes. Using overlapping hotspot regions in the soybean oil QTLs with collinearity, we mined for novel oil content-related genes. Overall, we identified 23 putatively functional genes associated with oil content in soybean and annotated them using a number of annotation databases. Our findings provide a valuable framework for elucidating evolutionary relationships between soybean oil-related QTLs and lay a foundation for functional marker-assisted breeding relating to soybean oil content.