QuEChERS-三重四极杆液相色谱质谱联用法快速筛查花生中52种农药残留

目的建立三重四极杆液相色谱质谱联用法快速筛选和确认花生中52种农药残留的分析方法。方法样品经0.1%甲酸乙腈提取,提取液经MgSO4、乙二胺-N-丙基硅烷化硅胶(primary secondary amine, PSA)和C18净化后,采用Waters C18 (100 mm×2.1 mm, 1.7μm)色谱柱分离,在三重四极杆正离子反应监测模式下测定,基质标准曲线结合外标法定量。结果 52种农药在0.001-0.1μg/mL范围内线性良好,相关系数均大于0.996,方法的定量限为1-5μg/kg,通过实际样品加标回收实验,52种农药在10μg/kg的加标回收率为76.4%-107.6%,相对标准偏差为3.5%-12.3%。结论该方法适用于花生中农药残留的筛查检测及定量分析。

Preliminary Study on Immuno-gold Chromatography Assay for Rapid Detection of Vibrio parahaemolyticus

[ Objective] Vibrio parahaemolyticus was chosen as the material to make rabbit anti- V. parahaemolyticus polyclonal antibody, the immuno-gold chromatography assay （IGCA） was used to develop immunogold labeling test strips for detecting V. parahaemolyticus. [Methods] Rabbit anti-V, parahaemolyticus IgG-2 was used to wrap nitrocellulose membrane detection line, goat anti-rabbit IgG was used to wrap control line, and im- muno-gold was used to label rabbit anti-V, parahaemolyticus IgG-1 to establish the immuno-gold chromatography assay （IGCA） for detection of V. parahaemolyticus. [Results] Test strip detection lines of positive results along with the control line were all red, test results could be got in only five to fifteen minutes, the minimum detectable amount of this method to V. parahaemolyticus was 3.60 x 104 cfu/ml, and cross reactions wouldnt occur when detecting common intestinal bacteria like Vibrio alginolyticus, vibrio cholerae, Vibrio damsela, Vibrio metschnikovii, Citrobacter freundii and salmonella etc. The test results were undifferentiated when the test strips were stored at 4 ~C for six months, stored at room temperature for three months or stored at 37 ~C for one month. [ Condusionl The established test strip assay was simple and rapid during operation, with high sensitivity, strong specificity and good stability, the test results were easy to be observed and judged, and this assay was very suitable for grassroots breeding department application.

高效液相色谱-串联质谱法快速测定鸡肉中阿奇霉素残留

[目的]建立高效液相色谱-串联质谱法测定鸡肉中阿奇霉素残留的检测方法。[方法]鸡肉样品采用乙腈提取后,加入5 g NaSO_(4),盐析后,用饱和乙腈正己烷溶液去除脂质层。净化后,采用Waters C_(18)(100 mm×2.1 mm×1.7μm)色谱柱分离,采用高效液相色谱-串联质谱法测定,基质标准曲线结合外标法定量。[结果]阿奇霉素在1.0~200.0μg/L线性关系良好(R^(2)=0.999)。在添加量为5、10、50μg/kg时,平均回收率分别为83.2%、91.2%、99.4%,相对标准偏差(RSD)分别为4.56%、4.72%、5.31%。[结论]该研究建立的方法精密度、准确度较高,选择性好,操作简便,适用于鸡肉中阿奇霉素残留的日常监测。

COⅠ和16S rRNA基因条形码在海马物种鉴定中的应用

本研究基于DNA条形码技术,应用通用引物扩增了6种海马的线粒体细胞色素氧化酶Ⅰ基因(COⅠ)和16S核糖体RNA基因(16S rRNA)部分序列,将测定的6种海马基因序列和GenBank数据库下载其他已知海马物种的参考序列进行系统发育分析。结果表明,COⅠ基因或16S rRNA基因可以对大部分海马物种进行准确鉴定,其中COⅠ基因具有更高的种间分辨率。对于亲缘关系较近的海马物种,应同时结合形态特征和地理分布等信息进一步鉴别。本研究提供的海马物种鉴定方案可对海马物种进行准确快速地鉴定,为打击海马走私、保护海马资源提供技术支持。