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杜长大猪UCP3基因克隆及白藜芦醇对其表达的影响 被引量:3
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作者 崔悦悦 张广杰 +8 位作者 徐晓芙 夏琴 邹辉 江雨航 瞿秋红 蒋钦杨 黄艳娜 郭亚芬 兰干球 《南方农业学报》 CAS CSCD 北大核心 2019年第3期439-445,共7页
【目的】明确白藜芦醇对杜长大猪UCP3基因表达及脂肪代谢的影响,为揭示猪优良肉质性状形成机制打下基础。【方法】提取2月龄杜长大猪皮下脂肪组织RNA,采用RT-PCR扩增杜长大猪UCP3基因编码区(CDS)序列,运用在线软件对其进行生物信息学分... 【目的】明确白藜芦醇对杜长大猪UCP3基因表达及脂肪代谢的影响,为揭示猪优良肉质性状形成机制打下基础。【方法】提取2月龄杜长大猪皮下脂肪组织RNA,采用RT-PCR扩增杜长大猪UCP3基因编码区(CDS)序列,运用在线软件对其进行生物信息学分析,并以实时荧光定量PCR检测白藜芦醇(400 mg/kg,饲喂30 d)对杜长大猪脂肪中UCP3基因表达的影响。【结果】从杜长大猪皮下脂肪组织中成功克隆获得UCP3基因CDS序列,全长927 bp,共编码308个氨基酸,其蛋白分子质量33673.09 Da,理论等电点(pI)9.44;与普通猪的UCP3基因序列相比,杜长大猪UCP3基因编码序列共有9处发生碱基突变,其中4处为错义突变,导致UCP3蛋白有4处氨基酸突变,分别为第74位脯氨酸(Pro)→亮氨酸(Leu)、第150位甘氨酸(Gly)→精氨酸(Arg)、第203位Arg→Gly和第259位缬氨酸(Val)→Leu。杜长大猪UCP3基因CDS序列与普通猪UCP3基因CDS序列的同源性为99.0%,其UCP3蛋白二级结构中以α-螺旋占比最高(46.43%)、β-转角占比最低(7.47%),属于亲水性蛋白。饲喂白藜芦醇(400 mg/kg)能极显著上调杜长大猪皮下脂肪UCP3基因表达(P<0.01),其相对表达量是对照组(不饲喂白藜芦醇)的2.06倍。【结论】白藜芦醇是通过上调杜长大猪UCP3基因表达而影响其脂肪代谢。 展开更多
关键词 杜长大猪 解偶联蛋白(UCP) UCP3基因 生物信息学分析 白藜芦醇 基因表达
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天津大神堂海域人工鱼礁区砂壳纤毛虫群落的季节变化
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作者 于莹 徐晓甫 +2 位作者 王硕 张博伦 张武昌 《海洋科学》 CAS CSCD 北大核心 2021年第11期96-104,共9页
于2015年夏季、秋季和2016年春季在天津大神堂海域人工鱼礁区进行砂壳纤毛虫群落的调查。3个季节共鉴定出砂壳纤毛虫10属31种,拟铃虫属(Tintinnopsis)种类最多,夏季砂壳纤毛虫种类最多。砂壳纤毛虫丰度、生物量和群落多样性指数存在明... 于2015年夏季、秋季和2016年春季在天津大神堂海域人工鱼礁区进行砂壳纤毛虫群落的调查。3个季节共鉴定出砂壳纤毛虫10属31种,拟铃虫属(Tintinnopsis)种类最多,夏季砂壳纤毛虫种类最多。砂壳纤毛虫丰度、生物量和群落多样性指数存在明显的季节差异。砂壳纤毛虫丰度夏季最高[(379±355)个/L],秋季最低[(173±190)个/L];生物量夏季最高[(2.74±2.82)μg C/L],秋季最低[(0.23±0.21)μg C/L]。砂壳纤毛虫壳的平均口径夏季最大[(49.64±6.90)μm],秋季最小(26.76±4.55μm)。砂壳纤毛虫群落Shannon多样性指数夏季最高(2.025±0.574),春季最低(0.922±0.437);Pielou均匀度指数秋季最高(0.682±0.276),春季最低(0.448±0.266)。春季鱼礁区和对照区砂壳纤毛虫优势种存在差异,夏季和秋季鱼礁区和对照区砂壳纤毛虫优势种相同;3个季节鱼礁区第Ⅰ优势种的优势度均明显高于对照区。3个季节鱼礁区砂壳纤毛虫丰度和生物量均高于对照区,这可能是由于投礁后砂壳纤毛虫的适口饵料——nano-浮游植物丰度增加导致;3个季节鱼礁区Shannon多样性指数和Pielou均匀度指数均低于对照区,可能是因为本次调查是在投礁1~2 a后开展的,时间较短,鱼礁区砂壳纤毛虫群落尚不稳定,鱼礁区独特生态系统的变化尚需更长的时间来进行跟踪监测。 展开更多
关键词 砂壳纤毛虫 群落结构 季节变化 人工鱼礁 大神堂海域
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退火温度对Zn0.3Fe2.7O4薄膜电磁性能的影响
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作者 林映燕 许晓赋 +1 位作者 高忠坚 高立华 《三明学院学报》 2020年第2期26-30,共5页
采用化学共沉淀法制备Zn0.3Fe2.7O4粉体,压制成高密度靶材,用射频磁控溅射法制备成膜。使用XRD、VSM、磁-电综合测试系统研究不同退火温度对样品磁性能及磁电阻的影响。结果表明:退火温度影响薄膜的晶化程度,600℃退火样品可得到较高的... 采用化学共沉淀法制备Zn0.3Fe2.7O4粉体,压制成高密度靶材,用射频磁控溅射法制备成膜。使用XRD、VSM、磁-电综合测试系统研究不同退火温度对样品磁性能及磁电阻的影响。结果表明:退火温度影响薄膜的晶化程度,600℃退火样品可得到较高的饱和磁化强度;磁电阻随着退火温度的升高大致呈先增加后减小的趋势;常温测得300℃退火样品,磁电阻达到最大值2.93%。 展开更多
关键词 锌铁氧体 退火温度 磁化强度 磁电阻
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Linking Structural Equation Modeling with Bayesian Network and Its Application to Coastal Phytoplankton Dynamics in the Bohai Bay
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作者 xu xiao-fu SUN Jian +2 位作者 NIE Hong-tao YUAN De-kui TAO Jian-hua 《China Ocean Engineering》 SCIE EI CSCD 2016年第5期733-748,共16页
Bayesian networks (BN) have many advantages over other methods in ecological modeling, and have become an increasingly popular modeling tool. However, BN are flawed in regard to building models based on inadequate e... Bayesian networks (BN) have many advantages over other methods in ecological modeling, and have become an increasingly popular modeling tool. However, BN are flawed in regard to building models based on inadequate existing knowledge. To overcome this limitation, we propose a new method that links BN with structural equation modeling (SEM). In this method, SEM is used to improve the model structure for BN. This method was used to simulate coastal phytoplankton dynamics in the Bohai Bay. We demonstrate that this hybrid approach minimizes the need for expert elicitation, generates more reasonable structures for BN models, and increases the BN model's accuracy and reliability. These results suggest that the inclusion of SEM for testing and verifying the theoretical structure during the initial construction stage improves the effectiveness of BN models, especially for complex eco-environment systems. The results also demonstrate that in the Bohai Bay, while phytoplankton biomass has the greatest influence on phytoplankton dynamics, the impact of nutrients on phytoplankton dynamics is larger than the influence of the physical environment in summer. Furthermore, although the Redfield ratio indicates that phosphorus should be the primary nutrient limiting factor, our results show that silicate plays the most important role in regulating phytoplankton dynamics in the Bohai Bay. 展开更多
关键词 structural equation modeling Bayesian networks ecological modeling Bohai Bay phytoplankton dynamics
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Polarization Beam Splitter Based on a Self-Collimation Michelson Interferometer in a Silicon Photonic Crystal
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作者 CHEN Xi-Yao LIN Gui-Min +5 位作者 LI Jun-Jun xu xiao-fu JIANG Jun-Zhen QIANG Ze-xuan QIU Yi-Shen LI Hui 《Chinese Physics Letters》 SCIE CAS CSCD 2012年第1期144-147,共4页
A polarization beam splitter based on a self-collimation Michelson interferometer (SMI) in a hole-type silicon photonic crystal is proposed and numerically demonstrated.Utilizing the polarization dependence of the tra... A polarization beam splitter based on a self-collimation Michelson interferometer (SMI) in a hole-type silicon photonic crystal is proposed and numerically demonstrated.Utilizing the polarization dependence of the transmission spectra of the SMI and polarization peak matching method,the SMI can work as a polarization beam splitter (PBS) by selecting an appropriate path length difference in the structure.Based on its novel polarization beam splitting mechanics,the polarization extinction ratios (PERs) for TM and TE modes are as high as 18.4 dB and 24.3 dB,respectively.Since its dimensions are only several operating wavelengths,the PBS may have practical applications in photonic integrated circuits. 展开更多
关键词 POLARIZATION Michelson INTERFEROMETER
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Corrosion life of artificial reef under natural seawater conditions
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作者 WANG Hong xu xiao-fu LIU Chang-gen 《Marine Science Bulletin》 CAS 2019年第1期16-26,共11页
Corrosion of steel bars with chloride salt erosion is one of the main reasons for the deterioration of the performance of reinforced concrete structures.The key to the service life of concrete members is the transport... Corrosion of steel bars with chloride salt erosion is one of the main reasons for the deterioration of the performance of reinforced concrete structures.The key to the service life of concrete members is the transport rate of chloride ions and the time for rusting of steel bars.In this paper,the artificial reef concrete member is taken as the research object,and the diffusion coefficient of seawater chloride ion in C30,C35 and steel slag composite artificial reef concrete is analyzed.The critical chloride ion concentration of steel corrosion in concrete is used as the boundary condition for life prediction.The chloride ion diffusion model predicts the corrosion life of C30,C35 and steel slag composite artificial reefs in seawater.The results show that the diffusion law of chloride ions in concrete artificial reefs basically meets Fick's second law.The corrosion life of C30,C35 and steel slag composite concrete reefs was calculated by the model to be 51.6,54.8 and 56.8 years,respectively. 展开更多
关键词 artificial reef CONCRETE chloride ion diffusion coefficient corrosion life
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The characteristic of phytoplankton community and its variation at artificial reef area of the coastal sea of Tianjin
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作者 ZHANG xue xu xiao-fu +3 位作者 FANG En-jun HOU Chun-qiang CHEN Wei ZHANG Bo-lun 《Marine Science Bulletin》 CAS 2019年第1期40-55,共16页
Three cruises were investigated respectively in May,July and November 2016 in the artificial reef area of 2010,2012,and 2014 and the non-artificial reef area(control area)in the coastal area of Tianjin.Each area had 3... Three cruises were investigated respectively in May,July and November 2016 in the artificial reef area of 2010,2012,and 2014 and the non-artificial reef area(control area)in the coastal area of Tianjin.Each area had 3 sampling stations,a total of 12 sites.The result showed that 58 taxa of phytoplankton were identified which belongs to 2 phyla 28 genera,Among these species,bacillariophyta including 19 genera 44 species,pyrrhophyta including 9 genera and 14 species.Give priority to with diatom species,accounting for 75.9%of the total species,followed by dinoflagellates,accounting for 24.1%.Dominant phytoplankton species give priority to with coscinodiscus and Chaetoceros of bacillariophyta,mainly including Coscinodiscus wailesii,C.asteromphalus,C.granii,C.oculus-iridis,C.spp.,Chaetoceros castracanei,C.lorenzianus,C.curvisetus,Pseudo-nitzschiapungens,Guianardia delicatula,Rhizosolenia setigera,Proboscia alata f.indica and Noctilluca scintillans.Phytoplankton cell abundance revealed obviously seasonal changes,the average cell abundance respectively was 94.79×10^4 cells/m^4、39.53×10^4 cells/m^4 and 21.5×10^4 cells/m^3.Phytoplankton diversity index has obviously seasonal variation,and in November,shannon-wiener diversity index,Margalef index and Pielou index are higher than in May and July.Comparing the artificial reef areas with the control area,the phytoplankton cells abundance of the control area in May was significantly higher than the artificial reef areas.In July and November,the artificial reef area of 2014 phytoplankton cell abundance was significantly higher than other artificial reef area and the control area.Diversity index of phytoplankton in the control area and the artificial reef of 2012 was lower than the artificial reef area of 2010 and 2014,and lowest in non-artificial reef areas.Obviously,the construction of artificial reef had significant effect for the improvement of the phytoplankton community diversity,but the phytoplankton community diversity is not always increasing and fluctuating to a certain extent as time goes by. 展开更多
关键词 PHYTOPLANKTON artificial reef community structure
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