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运脾开胃方对小鼠下丘脑神经元细胞NPY水平的干预作用及机制研究 被引量:2
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作者 胡云霞 倪永骋 +6 位作者 张彪 徐奚如 武相 张静 方媛 高慧 白宇 《中医药学报》 CAS 2023年第6期25-30,共6页
目的:观察运脾开胃方对小鼠下丘脑神经元细胞神经肽Y(NPY)的干预作用及其与Ghrelin受体(GHSR)的关系,研究运脾开胃方对中枢能量代谢途径的影响及机制。方法:培养永生化下丘脑神经元细胞,制备不同浓度的运脾开胃方水煎剂,运用CCK-8法筛... 目的:观察运脾开胃方对小鼠下丘脑神经元细胞神经肽Y(NPY)的干预作用及其与Ghrelin受体(GHSR)的关系,研究运脾开胃方对中枢能量代谢途径的影响及机制。方法:培养永生化下丘脑神经元细胞,制备不同浓度的运脾开胃方水煎剂,运用CCK-8法筛选药物浓度;采用激光共聚焦检测细胞内Ca^(2+)水平,免疫荧光检测细胞内GHSR水平,Western Blot法检测NPY、Sirt 1、mTOR、AMPK蛋白表达水平;采用GHSR抑制剂[D-Lys3]-GHRP-6(DLS)阻断GHSR。结果:运脾开胃方能在一定时间范围内上调下丘脑神经元细胞内的Ca^(2+)相对荧光强度;能够上调下丘脑神经元细胞上的GHSR、Sirt 1、NPY、p-mTOR水平,下调p-AMPK水平(P<0.05),且这种效应会被GHSR受体抑制剂(D-Lys3)-GHRP-6阻断(P<0.01)。结论:运脾开胃方可通过激活GHSR提高下丘脑神经元细胞NPY表达水平,其机制可能与Sirt 1、mTOR、AMPK有关。 展开更多
关键词 运脾开胃方 神经肽Y CA^(2+) GHRELIN GHSR
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论“病痰饮者,当以温药和之”及其临床运用 被引量:2
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作者 徐奚如 郑开明 《长春中医药大学学报》 2023年第1期32-35,共4页
痰饮是一类致病因素或病理产物的统称,可引起一系列类似的症状群,张仲景提出了“病痰饮者,当以温药和之”的治则。临床每遇症状复杂之疑难杂症,一旦辨为痰饮病,从该治法出发,以温药治痰饮病之本,以其它不同属性的药物治痰饮病之标,既治... 痰饮是一类致病因素或病理产物的统称,可引起一系列类似的症状群,张仲景提出了“病痰饮者,当以温药和之”的治则。临床每遇症状复杂之疑难杂症,一旦辨为痰饮病,从该治法出发,以温药治痰饮病之本,以其它不同属性的药物治痰饮病之标,既治痰饮病之共性,又治痰饮病之个性,疗效甚佳。通过从“温药和之”治疗痰饮病的条件、依据和临床意义分析,结合笔者临床感悟及实践,以飨同道。 展开更多
关键词 痰饮 温药和之 临床运用
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2000—2014年呼伦贝尔草原植被覆盖度时空变化分析 被引量:36
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作者 彭飞 范闻捷 +1 位作者 徐希孺 刘星 《北京大学学报(自然科学版)》 EI CAS CSCD 北大核心 2017年第3期563-572,共10页
以呼伦贝尔草原核心区的新巴尔虎右旗、新巴尔虎左旗、陈巴尔虎旗和鄂温克族自治旗为主要研究区,基于MODIS NDVI数据,利用像元二分模型反演得到植被覆盖度,并结合土地覆盖分类产品,构建2000—2014年研究区植被覆盖度时间序列。通过时间... 以呼伦贝尔草原核心区的新巴尔虎右旗、新巴尔虎左旗、陈巴尔虎旗和鄂温克族自治旗为主要研究区,基于MODIS NDVI数据,利用像元二分模型反演得到植被覆盖度,并结合土地覆盖分类产品,构建2000—2014年研究区植被覆盖度时间序列。通过时间序列分析,从不同的时间和空间尺度分析草原植被覆盖度的变化规律;同时引入覆盖度异常变化点检测算法,并结合该地区同期气象数据,进一步探讨研究区植被覆盖度变化与气象因子之间的内在驱动力关系。结果表明,植被覆盖度在空间分布上主要表现为:从东往西依次递减,特别是研究区西南部,覆盖度最低;15年来研究区植被年际变化总体上呈现前10年下降、后5年缓慢上升的趋势。对植被覆盖度的异常变化进行分析,结果显示:返青期和枯萎期覆盖度的剧烈变化与温度的相关性较大,生长旺季内(7—8)月覆盖度的剧烈变化主要与降水量有关。 展开更多
关键词 呼伦贝尔草原 像元二分模型 植被覆盖度(FVC) 时间序列 气象因子 植被时序变化点检测算法 相关性
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高速转子连接结构刚度损失及振动特性 被引量:14
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作者 洪杰 徐翕如 +1 位作者 苏志敏 马艳红 《北京航空航天大学学报》 EI CAS CSCD 北大核心 2019年第1期18-25,共8页
高负荷航空发动机转子的转速和支点跨度不断加大,使得转子弯曲刚度下降,并在工作中具有一定弯曲变形。转子弯曲变形时,连接界面会存在刚度损失,需考虑转子弯曲变形对连接界面刚度特性及转子系统振动特性的影响。提出了定量描述连接界面... 高负荷航空发动机转子的转速和支点跨度不断加大,使得转子弯曲刚度下降,并在工作中具有一定弯曲变形。转子弯曲变形时,连接界面会存在刚度损失,需考虑转子弯曲变形对连接界面刚度特性及转子系统振动特性的影响。提出了定量描述连接界面刚度损失的力学模型,并针对非连续转子系统的动力学设计,提出了基于应变能分布优化的连接结构刚度损失抑制方法。数值仿真结果表明:转子弯曲变形下,连接界面刚度损失显著,会使转子弯曲临界转速大幅降低;通过转子应变能分布优化设计可有效降低连接界面刚度损失对转子系统振动特性的影响,对转子系统振动特性优化设计具有重要的指导意义。 展开更多
关键词 连接界面 刚度损失 结构非连续性 转子系统 振动特性 应变能
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系统生物学在认知功能障碍研究中的应用 被引量:1
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作者 徐奚如 张彪 顾晓群 《生物技术进展》 2020年第2期124-129,共6页
认知功能障碍可导致患者日常生活能力、社会适应能力明显下降,严重影响患者的生活质量。根据近年来众多研究显示,认知功能障碍的发生发展与基因、蛋白质、微生物等内环境因素的失调和紊乱有关。基于系统生物学的研究,梳理了近年来国内... 认知功能障碍可导致患者日常生活能力、社会适应能力明显下降,严重影响患者的生活质量。根据近年来众多研究显示,认知功能障碍的发生发展与基因、蛋白质、微生物等内环境因素的失调和紊乱有关。基于系统生物学的研究,梳理了近年来国内外研究学者在代谢组学、蛋白质组学、基因组学和肠道微生物组学层面对认知功能障碍的研究,并对系统生物学在认知功能障碍中的应用前景进行了展望,以期为认知功能障碍相关研究提供参考。 展开更多
关键词 系统生物学 认知功能障碍 代谢组学 蛋白质组学 基因组学 肠道微生态
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糖脂清方对高糖诱导HT22细胞损伤的保护作用 被引量:2
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作者 石崯力 王旭 +3 位作者 赵云 邱玲艳 徐奚如 孙斯凡 《中医药信息》 2022年第3期7-15,共9页
目的:探讨“糖脂清”对高糖诱导的HT22细胞损伤的保护作用机制。方法:将Wistar大鼠随机分成正常组和“糖脂清”、二甲双胍含药血清组,依据成人临床剂量进行灌胃,灌胃后取每组大鼠腹主动脉血并进行离心、灭活、过滤,由此制备不同剂量的... 目的:探讨“糖脂清”对高糖诱导的HT22细胞损伤的保护作用机制。方法:将Wistar大鼠随机分成正常组和“糖脂清”、二甲双胍含药血清组,依据成人临床剂量进行灌胃,灌胃后取每组大鼠腹主动脉血并进行离心、灭活、过滤,由此制备不同剂量的“糖脂清”和二甲双胍大鼠含药血清。通过CCK-8法筛选干预HT22细胞最适造模高糖浓度和最佳干预时间,将生长稳定的HT22细胞分为空白组、模型组、二甲双胍含药血清组、低剂量含药血清组、中剂量含药血清组和高剂量含药血清组,造模组采用最适高糖浓度进行诱导,再对高糖环境下的HT22细胞给予“糖脂清”高、中、低剂量和二甲双胍含药血清进行干预,检测各组HT22细胞氧化应激指标超氧化物歧化酶(SOD)、丙二醛(MDA)和乳酸脱氢酶(LDH)含量;免疫荧光法和Hoechst 33342染色法检测神经元凋亡和Lamp2的表达;WesternBlot法检测各组HT22细胞凋亡、自噬相关蛋白Bax、Bcl-2、Caspase-3、SIRT1和JNK表达水平。结果:“糖脂清”能够调节和改善小鼠海马HT22细胞在长期高糖环境下的生长和形态变化,升高SOD含量(P<0.01),降低MDA和LDH水平(P<0.01),对HT22细胞遭受的氧化损伤起到一定程度的保护作用;与模型组相比,“糖脂清”的干预可下调炎症凋亡、自噬相关因子Bax、Caspase-3、SIRT1、JNK蛋白的表达,上调Bcl-2、Lamp2蛋白表达水平(P<0.05)。结论:“糖脂清”可显著缓解海马区域神经元凋亡和氧化应激异常状态,并在一定程度上抑制细胞自噬,调控自噬体与溶酶体的结合过程,这些结果为“糖脂清”成为改善2型糖尿病合并认知功能的有效药物提供了全新的思路和方向。 展开更多
关键词 中医药 HT22细胞 糖尿病认知功能障碍 细胞凋亡 细胞自噬
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冰菖散吸嗅剂制备及其对MCI模型小鼠的干预效果研究 被引量:2
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作者 周春梅 顾晓群 +2 位作者 白宇 徐奚如 张彪 《中国野生植物资源》 CSCD 2021年第3期1-6,共6页
目的:使冰菖散吸嗅剂更加科学规范并验证其对轻度认知障碍(Mild cognitive impairment,MCI)模型小鼠的干预效果。方法:采用正交试验法,以挥发油的提取量为考察指标,优选挥发油最佳提取工艺条件,并采用GC-MS技术分析其主要化学成分,再加... 目的:使冰菖散吸嗅剂更加科学规范并验证其对轻度认知障碍(Mild cognitive impairment,MCI)模型小鼠的干预效果。方法:采用正交试验法,以挥发油的提取量为考察指标,优选挥发油最佳提取工艺条件,并采用GC-MS技术分析其主要化学成分,再加入冰片制备成冰菖散吸嗅剂,经鼻给药两周后检测其对MCI小鼠的筑巢行为以及β-淀粉样蛋白(Amyloidβ-protein,Aβ)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)等指标的影响。结果:(1)最佳提取工艺为:药材加入17.5倍水量,2%NaCl,50℃水浴3 h;经GC-MS分析,冰菖散混合挥发油成分中丁香酚的相对含量超过一半。(2)冰菖散吸嗅剂显著提高5.5月龄MCI小鼠的筑巢质量。(3)中药组(冰菖散嗅吸剂)血清TNF-α浓度显著降低,与模型组相比差异非常显著(P<0.001)。(4)病理切片显示:5.5月龄时,模型组MCI模型小鼠的脑皮层和海马均出现Aβ斑块,中药组(冰菖散嗅吸剂)皮层以及海马均没有Aβ沉积,而西药组(多奈哌齐)只有皮层中存在少量Aβ沉积,与模型组相比差异非常显著(P<0.001)。结论:优选出的提取工艺出油量较高,且稳定可行;冰菖散吸嗅剂经鼻给药可以减少Aβ沉积、降低血清TNF-α浓度从而提高5.5月龄MCI模型小鼠的认知功能。 展开更多
关键词 正交试验 气质联用 筑巢实验 Β-淀粉样蛋白 肿瘤坏死因子-α
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从心智为用和肾精为体角度探讨认知的功能和物质基础
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作者 张彪 徐奚如 顾晓群 《中国临床研究》 CAS 2023年第5期755-757,共3页
中医基础理论认为,心主神明,肾精充养脑髓,心肾两脏与脑的认知功能紧密相关,心-肾功能失调可致髓减脑消、神机失用之中医内科学之“呆病”,即认知功能障碍这类进行性神经退行性疾病。笔者认为,心主神明,为智,为功能,为用;肾藏精,生髓,... 中医基础理论认为,心主神明,肾精充养脑髓,心肾两脏与脑的认知功能紧密相关,心-肾功能失调可致髓减脑消、神机失用之中医内科学之“呆病”,即认知功能障碍这类进行性神经退行性疾病。笔者认为,心主神明,为智,为功能,为用;肾藏精,生髓,为物质基础,为体。在认知功能障碍的治疗中,激发心主神明之功能、补充肾精之物质基础是主要治法。笔者在前期研究中,亦发现名老中医治疗认知功能障碍类疾病,最喜用心经药物,并佐以补肾之品。故本文主要从体用角度,结合生理病理及现代医学进展,阐述中医基础理论中心、肾两脏与认知的关系,为临床治疗认知功能障碍提供思路。 展开更多
关键词 心肾相交 体用关系 认知障碍 神经退行性疾病
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The concept of effective emissivity of nonisothermal mixed pixel and its test 被引量:17
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作者 CHEN Liangfu ZHUANG Jiali +3 位作者 xu xiru NIU Zheng ZHANG Renhua XIANG Yueqing 《Chinese Science Bulletin》 SCIE EI CAS 2000年第9期788-795,共8页
Based on two preconditions, the local thermal equilibrium is satisfied and emissivities donot change with temperature, the concept of component effective emissivity of nonisothermal mixed pixel has been put forward an... Based on two preconditions, the local thermal equilibrium is satisfied and emissivities donot change with temperature, the concept of component effective emissivity of nonisothermal mixed pixel has been put forward and then the radiant directionality model of nonisothermal mixed pixel constructed from it. Our study shows that the component effective emissivity is associated with geometric structure, optical properties of target, and viewing angle, but does not depend on the component temepratures. The component temperatures can only change the ratio of component radiance to the total radiance of the mixed-pixel. The total effective emissivity of this pixel is the complement of its directional-hemisphere reflectance. After the simulation of component effective emissivity of the discrete cones and continuous vegetation canopy (winter wheat) by the Monte Carlo method, our model of radiant directionality of nonisothermal mixed pixel have been proved by lab and field measurements. 展开更多
关键词 NONISOTHERMAL MIXED PIXEL component EFFECTIVE EMISSIVITY multiple scattering.
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The spatial scaling effect of continuous canopy Leaves Area Index retrieved by remote sensing 被引量:13
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作者 xu xiru FAN WenJie TAO Xin 《Science China Earth Sciences》 SCIE EI CAS 2009年第3期393-401,共9页
Leave Area Index (LAI) is one of the most basic parameters to describe the geometric structure of plant canopies. It is also important input data for climatic model and interaction model between Earth surface and atmo... Leave Area Index (LAI) is one of the most basic parameters to describe the geometric structure of plant canopies. It is also important input data for climatic model and interaction model between Earth surface and atmosphere, and some other things. The spatial scaling of retrieved LAI has been widely studied in recent years. Based on the new canopy reflectance model, the mechanism of the scaling effect of con- tinuous canopy Leaf Area Index is studied, and the scaling transform formula among different scales is found. Both the numerical simulation and the field validation show that the scale transform formula is reliable. 展开更多
关键词 spatial scaling EFFECT remotely sensed LAI the TRUE LAI the reflectance model of CONTINUOUS CANOPY
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Crop area and leaf area index simultaneous retrieval based on spatial scaling transformation 被引量:6
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作者 Fan WenJie Yan BinYan xu xiru 《Science China Earth Sciences》 SCIE EI CAS 2010年第11期1709-1716,共8页
Accurate estimation of crop yields is crucial for ensuring food security. However, crops are distributed so fragmentally in China that mixed pixels account for a large proportion in moderate and coarse resolution remo... Accurate estimation of crop yields is crucial for ensuring food security. However, crops are distributed so fragmentally in China that mixed pixels account for a large proportion in moderate and coarse resolution remote sensing images. As a result, unmixing of mixed pixel becomes a major problem to estimate crop yield by means of remote sensing method. Aimed at mixed pixels, we developed a new method to introduce additional information contained in the spatial scaling transformation equation to the canopy reflectance model. The crop area and LAI can be retrieved simultaneously. On the basis of a precise and simple canopy reflectance model, directional second derivative method was chosen to retrieve LAI from optimal bands of hyper-spectral data; this method can reduce the impact of the canopy non-isotropic features and soil background. To evaluate the performance of the method, Yingke Oasis, Zhangye City, Gansu Province, was chosen as the validation area. This area was covered mainly by maize and wheat. A Hyperion/EO-1 image with the 30 m spatial resolution was acquired on July 15, 2008. Images of 180 m and 1080 m resolutions were generated by linearly interpolating the original Hyperion image to coarser resolutions. Then a multi-scale image serial was obtained. Using the proposed method, we calculated crop area and the average LAI of every 1080 m pixel. A SPOT-5 classification figure serves as the validation data of crop area proportion. Results show that the pattern of crop distribution accords with the classification figure. The errors are restrained mainly to -0.1-0.1, and approximate a Normal Distribution. Meanwhile, 85 LAI values obtained using LAI-2000 Plant Canopy Analyzer, equipped with GPS, were taken as the ground reference. Results show that the standard deviation of the errors is 0.340. The method proposed in the paper is reliable. 展开更多
关键词 scale TRANSFORMATION remote sensing CROP YIELD estimation SIMULTANEOUS RETRIEVAL CROP area LEAF area index
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A unified model of bidirectional reflectance distribution function for the vegetation canopy 被引量:6
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作者 xu xiru FAN WenJie +2 位作者 LI JuCai ZHAO Peng CHEN GaoXing 《Science China Earth Sciences》 SCIE EI CAS CSCD 2017年第3期463-477,共15页
An accurate and operational bidirectional reflectance distribution function (BDRF) canopy model is the basis of quantitative vegetation remote sensing. The canopy reflectance should be approximated as the sum of the... An accurate and operational bidirectional reflectance distribution function (BDRF) canopy model is the basis of quantitative vegetation remote sensing. The canopy reflectance should be approximated as the sum of the single scattering reflectance arising from the sun, pl, and the multiple scattering reflectance arising from the canopy, fin, as their directional characteristics are dramatically different. Based on the existing BRDF model, we obtain a new analytical expression of ρ1 and ρm in this paper, which is suitable for different illumination conditions and different vegetation canopies. According to the geometrical optic model at the leaf scale, the anisotropy of ρ1 can be ascribed to the geometry of the object, sun and the sensor, multiple scale clumping, and the fraction of direct solar radiation and diffuse sky radiation. Then, we parameterize the area ratios of four components: the sunlit foliage, sunlit ground, shadow foliage and shadow ground based on a Poisson distribution, and develop a new approximate analytical single scattering reflectance model. Assuming G=0.5, a recollision probability theory based scattering model is developed which considers the effects of diffuse sky radiation, scattering inside the canopy and rebounds between the canopy and soil. Validation using ground measurements of maize and black spruce forest proves the reliability of the model. 展开更多
关键词 Vegetation BRDF Unified model Clumping Index Proportion of direct solar radiation and diffuse sky radiation Recollision probability
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A unified canopy bidirectional reflectance (BRDF) model for row ceops 被引量:3
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作者 YAN BinYan xu xiru FAN WenJie 《Science China Earth Sciences》 SCIE EI CAS 2012年第5期824-836,共13页
Row sowing is a basic crop sowing method in China,and thus an accurate Bidirectional Reflectance Distribution Function (BRDF) model of row crops is the foundation for describing the canopy bidirectional reflectance ch... Row sowing is a basic crop sowing method in China,and thus an accurate Bidirectional Reflectance Distribution Function (BRDF) model of row crops is the foundation for describing the canopy bidirectional reflectance characteristics and estimating crop ecological parameters.Because of the macroscopically geometric difference,the row crop is usually regarded as a transition between continuous and discrete vegetation in previous studies.Were row treated as the unit for calculating the four components in the Geometric Optical model (GO model),the formula would be too complex and difficult to retrieve.This study focuses on the microscopic structure of row crops.Regarding the row crop as a result of leaves clumped at canopy scale,we apply clumping index to link continuous vegetation and row crops.Meanwhile,the formula of clumping index is deduced theoretically.Then taking leaf as the basic unit,we calculate the four components of the GO model and develop a BRDF model for continuous vegetation,which is gradually extended to the unified BRDF model for row crops.It is of great importance to introduce clumping index into BRDF model.In order to evaluate the performance of the unified BRDF model,the canopy BRDF data collected in field experiment,"Watershed Allied Telemetry Experiment Research (WATER)",from May 30th to July 1st,2008 are used as the validation dataset for the simulated values.The results show that the unified model proposed in this paper is able to accurately describe the non-isotropic characteristics of canopy reflectance for row crops.In addition,the model is simple and easy to retrieve.In general,there is no irreconcilable conflict between continuous and discrete vegetation,so understanding their common and individual characteristics is advantageous for simulating canopy BRDF.It is proven that the four components of the GO model is the basic motivational factor for bidirectional reflectance of all vegetation types. 展开更多
关键词 row crop BRDF CLUMPING clumping index
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Validation methods of LAI products on the basis of scaling effect 被引量:2
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作者 LIAO Yanran GAI Yingying +3 位作者 YAO Yanjuan FAN Wenjie xu xiru YAN Binyan 《遥感学报》 EI CSCD 北大核心 2015年第1期134-142,共9页
Validation is one of the most important processes used to evaluate whether remotely sensed products can accurately reflect land surface configuration. Leaf Area Index( LAI) is a key parameter that represents vegetatio... Validation is one of the most important processes used to evaluate whether remotely sensed products can accurately reflect land surface configuration. Leaf Area Index( LAI) is a key parameter that represents vegetation canopy structures and growth conditions. Accurate evaluation of LAI products is the basis for applying them to land surface models. In this study,validation methods of coarse resolution MODIS and GLASS LAI products for heterogeneous pixels are established on the basis of the scaling effect and the scaling transformation. Considering spatial heterogeneity and growth difference,we transformed LAI from field measurements into a 1 km resolution scale with the aid of middle resolution images. We used average LAI and apparent LAI separately to validate the algorithms and products of MODIS and GLASS LAI. Two study areas,Hebi City and the Yingke Oasis,were selected for validation. Both MODIS and GLASS LAI products underestimate the true LAI in crop area. However,this result cannot be completely attributed to their algorithms. Instead,the primary reason is the heterogeneity and nonuniformity of the coarse pixels.Underestimation is evident in the Yingke Oasis,where heterogeneity is significant. Given that GLASS LAI product is the fusion of multiple LAI products,the mean value of this product is closer to the real situation,but the dynamic range is narrower than that of MODIS LAI product. 展开更多
关键词 VALIDATION leaf area index scaling effect scaling transformation
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The spatial scaling effect of the discrete-canopy effective leaf area index retrieved by remote sensing 被引量:5
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作者 FAN WenJie GAI YingYing +1 位作者 xu xiru YAN BinYan 《Science China Earth Sciences》 SCIE EI CAS 2013年第9期1548-1554,共7页
The leaf area index(LAI) is a critical biophysical variable that describes canopy geometric structures and growth conditions.It is also an important input parameter for climate,energy and carbon cycle models.The scali... The leaf area index(LAI) is a critical biophysical variable that describes canopy geometric structures and growth conditions.It is also an important input parameter for climate,energy and carbon cycle models.The scaling effect of the LAI has always been of concern.Considering the effects of the clumping indices on the BRDF models of discrete canopies,an effective LAI is defined.The effective LAI has the same function of describing the leaf density as does the traditional LAI.Therefore,our study was based on the effective LAI.The spatial scaling effect of discrete canopies significantly differed from that of continuous canopies.Based on the directional second-derivative method of effective LAI retrieval,the mechanism responsible for the spatial scaling effect of the discrete-canopy LAI is discussed and a scaling transformation formula for the effective LAI is suggested in this paper.Theoretical analysis shows that the mean values of effective LAIs retrieved from high-resolution pixels were always equal to or larger than the effective LAIs retrieved from corresponding coarse-resolution pixels.Both the conclusions and the scaling transformation formula were validated with airborne hyperspectral remote sensing imagery obtained in Huailai County,Zhangjiakou,Hebei Province,China.The scaling transformation formula agreed well with the effective LAI retrieved from hyperspectral remote sensing imagery. 展开更多
关键词 The spatial scaling effect of the discrete-canopy effective leaf area index retrieved by remote sensing
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