Objective:To study the key target genes and signaling pathways in the treatment of Allergic Rhinitis(AR)with Radix Aconiti Lateralis Preparata(aka Fuzi).Methods:The TCMPS and PubChem databases were used to screen the ...Objective:To study the key target genes and signaling pathways in the treatment of Allergic Rhinitis(AR)with Radix Aconiti Lateralis Preparata(aka Fuzi).Methods:The TCMPS and PubChem databases were used to screen the active ingredients and target genes of Fuzi using oral bioavailability and drug similarity as screening conditions,and the GeneCards database was used to screen the target genes of AR.The online tool Venny2.1 was used to screen the target genes of Fuzi for the treatment of Allergic Rhinitis;the STRING database was used to obtain the protein-protein interaction(PPI)network of drug-disease targets,and the key target genes were identified by the MCC algorithm.The potential biological processes and signaling pathways were identified by GO enrichment and KEGG enrichment analysis.Finally,animal experiments were conducted to demonstrate the therapeutic effect ofFuzi on Allergic Rhinitis.Results:The TCMSP,PubChem and GeneCards databases were used to screen the 21 active compound components of Fuzi and 68 potential therapeutic target genes of Fuzi for Allergic Rhinitis.PPI network analysis identified the top ten key target genes,namely:PTGS2,TNF,IL6,AKT1,ALB,STAT3,CCL2,CXCL8,VEGFA and JUN,GO functional and KEGG pathway enrichment analysis showed that the significantly enriched functions and pathways of Fuzi on Allergic Rhinitis were closely related to Allergic Rhinitis.Finally,animal experiments were conducted to verify that Fuzi is effective in the treatment of Allergic rhinitis.Conclusion:Increased expression of IL-6 and TNF-αin nasal mucosal tissues of patients with Allergic Rhinitis was positively correlated with indicators related to the disease activity of AllergicRhinitis.Fuzi ameliorated the inflammatory changes in mice with Allergic Rhinitis by inhibiting the activation of Toll-like signaling pathway in the nasal mucosa and decreasing the expression activity of IL-6 and TNF-α.展开更多
目的旨在探讨髋部双能X线吸收测量仪(dual energy X-ray absorptiometer,DXA)检查体位对骨密度测量结果的影响。方法采用美国Hologic公司Discovery-Wi型DXA,对292例≥50岁男性和绝经后女性骨质疏松志愿者,分别进行两次不同摆位的左侧髋...目的旨在探讨髋部双能X线吸收测量仪(dual energy X-ray absorptiometer,DXA)检查体位对骨密度测量结果的影响。方法采用美国Hologic公司Discovery-Wi型DXA,对292例≥50岁男性和绝经后女性骨质疏松志愿者,分别进行两次不同摆位的左侧髋部DXA扫描:(1)内旋位:股骨干连同双脚一起内旋15~25°;(2)自然位:股骨干连同双脚不内旋或自然摆放。采用世界卫生组织(World Health Organization,WHO)亚洲人骨质疏松诊断标准,对两次扫描分析后得到的股骨颈、大转子、转子间、总面积和质量、Ward's区的DXA结果数据进行详细对比分析。结果内旋位即股骨干连同双脚一起内旋15~25°的髋部DXA扫描,得到不含有小转子结构的图像;而自然位即股骨干连同双脚不内旋或自然摆放的髋部DXA扫描,得到含有小转子结构的图像。自然位DXA扫描的转子骨密度、总骨矿盐含量、总骨密度均高于内旋位测定结果,但差异无统计学意义(均P>0.05)。内旋位和自然位体位下转子T值(-1.23±0.68 vs.-0.45±0.74)和Z值(-0.63±0.82 vs.0.16±0.94)、总T值(-1.12±0.77 vs.-0.74±0.73)和总Z值(-0.48±0.89 vs.-0.05±0.95)的差异有统计学意义(P<0.001)。两种不同体位DXA检查半定量分析结果显示:DXA自然位的WHO分类评分高于内旋位(2.65±0.49)分vs.(1.74±0.45)分],相应的骨折风险低于内旋位(1.35±0.49)分vs.(2.26±0.45)分]。结论内旋位是标准的检查方法,自然位DXA检查可能会“高估”骨密度,“低估”骨折风险。展开更多
基金Natural Science Foundation of Hainan Province(No.820RC627)。
文摘Objective:To study the key target genes and signaling pathways in the treatment of Allergic Rhinitis(AR)with Radix Aconiti Lateralis Preparata(aka Fuzi).Methods:The TCMPS and PubChem databases were used to screen the active ingredients and target genes of Fuzi using oral bioavailability and drug similarity as screening conditions,and the GeneCards database was used to screen the target genes of AR.The online tool Venny2.1 was used to screen the target genes of Fuzi for the treatment of Allergic Rhinitis;the STRING database was used to obtain the protein-protein interaction(PPI)network of drug-disease targets,and the key target genes were identified by the MCC algorithm.The potential biological processes and signaling pathways were identified by GO enrichment and KEGG enrichment analysis.Finally,animal experiments were conducted to demonstrate the therapeutic effect ofFuzi on Allergic Rhinitis.Results:The TCMSP,PubChem and GeneCards databases were used to screen the 21 active compound components of Fuzi and 68 potential therapeutic target genes of Fuzi for Allergic Rhinitis.PPI network analysis identified the top ten key target genes,namely:PTGS2,TNF,IL6,AKT1,ALB,STAT3,CCL2,CXCL8,VEGFA and JUN,GO functional and KEGG pathway enrichment analysis showed that the significantly enriched functions and pathways of Fuzi on Allergic Rhinitis were closely related to Allergic Rhinitis.Finally,animal experiments were conducted to verify that Fuzi is effective in the treatment of Allergic rhinitis.Conclusion:Increased expression of IL-6 and TNF-αin nasal mucosal tissues of patients with Allergic Rhinitis was positively correlated with indicators related to the disease activity of AllergicRhinitis.Fuzi ameliorated the inflammatory changes in mice with Allergic Rhinitis by inhibiting the activation of Toll-like signaling pathway in the nasal mucosa and decreasing the expression activity of IL-6 and TNF-α.
文摘目的旨在探讨髋部双能X线吸收测量仪(dual energy X-ray absorptiometer,DXA)检查体位对骨密度测量结果的影响。方法采用美国Hologic公司Discovery-Wi型DXA,对292例≥50岁男性和绝经后女性骨质疏松志愿者,分别进行两次不同摆位的左侧髋部DXA扫描:(1)内旋位:股骨干连同双脚一起内旋15~25°;(2)自然位:股骨干连同双脚不内旋或自然摆放。采用世界卫生组织(World Health Organization,WHO)亚洲人骨质疏松诊断标准,对两次扫描分析后得到的股骨颈、大转子、转子间、总面积和质量、Ward's区的DXA结果数据进行详细对比分析。结果内旋位即股骨干连同双脚一起内旋15~25°的髋部DXA扫描,得到不含有小转子结构的图像;而自然位即股骨干连同双脚不内旋或自然摆放的髋部DXA扫描,得到含有小转子结构的图像。自然位DXA扫描的转子骨密度、总骨矿盐含量、总骨密度均高于内旋位测定结果,但差异无统计学意义(均P>0.05)。内旋位和自然位体位下转子T值(-1.23±0.68 vs.-0.45±0.74)和Z值(-0.63±0.82 vs.0.16±0.94)、总T值(-1.12±0.77 vs.-0.74±0.73)和总Z值(-0.48±0.89 vs.-0.05±0.95)的差异有统计学意义(P<0.001)。两种不同体位DXA检查半定量分析结果显示:DXA自然位的WHO分类评分高于内旋位(2.65±0.49)分vs.(1.74±0.45)分],相应的骨折风险低于内旋位(1.35±0.49)分vs.(2.26±0.45)分]。结论内旋位是标准的检查方法,自然位DXA检查可能会“高估”骨密度,“低估”骨折风险。
