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哈维氏弧菌引起的条纹海马表皮溃疡综合征的研究 被引量:4
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作者 李营 王波 +5 位作者 张培玉 刘洪展 郑风荣 王宗兴 徐宗军 赵超 《水产学报》 CAS CSCD 北大核心 2019年第5期1298-1307,共10页
近年来,条纹海马的养殖在我国发展迅速,但由于多种养殖疾病的频繁暴发,严重制约了条纹海马养殖业的健康发展。自2017年以来,我国北方地区在条纹海马的养殖过程中,多次发生表皮溃疡综合征,患病海马游泳能力减弱,皮肤大面积溃疡,同时伴随... 近年来,条纹海马的养殖在我国发展迅速,但由于多种养殖疾病的频繁暴发,严重制约了条纹海马养殖业的健康发展。自2017年以来,我国北方地区在条纹海马的养殖过程中,多次发生表皮溃疡综合征,患病海马游泳能力减弱,皮肤大面积溃疡,同时伴随烂尾症状,解剖后发现其肠道发白,肝脏及鳃颜色暗淡,死亡率较高,给海马养殖业造成巨大的经济损失。本研究从患病条纹海马的溃烂肌肉及肠道中分离出13株优势菌株,经回归感染实验证实菌株HDM-2可引起条纹海马出现表皮溃疡综合征的类似症状,且半数致死量(LD_(50))约为2.89×10~8 CFU/mL。为确定菌株HDM-2的分类地位,采用生理生化及基于16S rDNA的分子生物学方法对其进行鉴定,结果显示,HDM-2为哈维氏弧菌;为了解菌株HDM-2的菌体特征,采用透射电镜(TEM)对菌株HDM-2进行观察,结果显示,HDM-2为周生鞭毛,有荚膜,大小约2.20μm×4.75μm;为获知HDM-2的抗生素敏感性,采用纸片扩散法进行药敏试验,结果显示,菌株HDM-2对氟苯尼考和头孢他啶等较敏感;患病条纹海马的组织病理学研究表明,菌株HDM-2对条纹海马的皮肤、肝脏以及鳃组织损伤较严重。本文可为条纹海马表皮溃疡综合征的防治及深入研究提供基础资料。 展开更多
关键词 条纹海马 表皮溃疡综合征 病原菌 哈维氏弧菌 药敏试验
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蔓荆子黄素对人非小细胞肺癌细胞H322的作用及机制研究 被引量:6
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作者 李遂新 雷光焰 +3 位作者 马晓军 杨得振 徐宗君 陈正浤 《现代肿瘤医学》 CAS 2020年第7期1072-1076,共5页
目的:探究蔓荆子黄素对人非小细胞肺癌细胞H322增殖和周期的影响及机制。方法:选择体外培养的人非小细胞肺癌H322细胞,给不同浓度(0,1,10,20,40μmol/L)蔓荆子黄素溶液作用不同时间(24 h,48 h,72 h)后,采取MTT法检测细胞的增殖情况、流... 目的:探究蔓荆子黄素对人非小细胞肺癌细胞H322增殖和周期的影响及机制。方法:选择体外培养的人非小细胞肺癌H322细胞,给不同浓度(0,1,10,20,40μmol/L)蔓荆子黄素溶液作用不同时间(24 h,48 h,72 h)后,采取MTT法检测细胞的增殖情况、流式细胞仪检测细胞周期、Western blot法检测CDK1、c-myc和survivin基因的表达情况。结果:不同浓度组的蔓荆子黄素溶液作用H322细胞(24 h,48 h,72 h)后均能抑制其增殖(P<0.05),且表现出剂量、时间依赖性;蔓荆子黄素通过影响H322细胞的增殖,使得细胞周期阻滞在G 2/M期,同时发现c-myc和survivin的表达与蔓荆子黄素浓度成负相关,CDK1的表达随着蔓荆子黄素浓度的升高先升高后降低。结论:蔓荆子黄素可能通过抑制CDK1、c-myc和survivin的表达,阻滞细胞周期在G 2/M期,进而抑制人非小细胞肺癌细胞H322的增殖并促进凋亡。 展开更多
关键词 蔓荆子黄素 非小细胞肺癌 增殖 凋亡 CDK1 C-MYC SURVIVIN
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微塑料对浮游植物群落的影响研究 被引量:1
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作者 李宇佳 孙萍 +3 位作者 张翔宇 徐宗军 王清爽 王宗灵 《长春理工大学学报(自然科学版)》 2022年第5期107-116,共10页
微塑料对浮游植物群落影响研究与海洋环境保护息息相关。搭载向阳红18科考船,随船搭建船基围隔并通过现场采集表层海水,开展不同粒径尺寸、浓度梯度微塑料对小型浮游植物、微型浮游植物以及微微型浮游植物生长影响的实验研究,探索了更... 微塑料对浮游植物群落影响研究与海洋环境保护息息相关。搭载向阳红18科考船,随船搭建船基围隔并通过现场采集表层海水,开展不同粒径尺寸、浓度梯度微塑料对小型浮游植物、微型浮游植物以及微微型浮游植物生长影响的实验研究,探索了更加贴近海洋实际条件下微塑料对浮游植物群落生长影响规律。研究发现微塑料对绝大多数海洋浮游植物群落具有抑制作用,但在特定条件下却表现出一定促进作用。该发现为进一步保护海洋环境及后续实验研究提供参考。 展开更多
关键词 微塑料 海洋 浮游植物群落 影响
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Changes of nutrient concentrations and N:P:Si ratios and their possible impacts on the Huanghai Sea ecosystem 被引量:15
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作者 FU Mingzhu WANG Zongling +2 位作者 PU Xinming xu zongjun ZHU Mingyuan 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第4期101-112,共12页
To investigate the impacts of nutrient concentrations and N:P:Si ratios on the ecosystem of the Huanghai Sea (Yellow Sea), the current status and long-term variation of nutrients concentrations and ratios as well ... To investigate the impacts of nutrient concentrations and N:P:Si ratios on the ecosystem of the Huanghai Sea (Yellow Sea), the current status and long-term variation of nutrients concentrations and ratios as well as phytoplankton community structure in the Huanghai Sea were collected and analyzed. The results reveal great annual and seasonal fluctuations in the nutrient concentrations and N:P:Si ratios during 1998-2008 with no clear pattern observed in the whole region. Yet on a seasonal scale of spring and in the coastal regions such as the Jiaozhou Bay and Sanggou Bay, the increase of DIN concentration and N:P ratio as well as the decrease of phosphate and sili- cate concentrations and Si:N ratios were relatively significant. Many pelagic ecosystem changes have occurred concurrent with these changes of the nutrient regime, such as the recent increase of primary production, changes of phytoplankton chlorophyll a biomass and abundance, an increase of eutrophication, and occurrence of HABs. In addition, new trends in the variation of nutrients seem to be developing in some particular transect such as 36N, which suggests that long-term and systematic ecosystem monitoring in the Huanghai Sea is necessary. 展开更多
关键词 nutrient concentration and ratio long-term variation Huanghai Sea ecosystemresponse
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Interspecies competition for nutrients between Prorocentrum donghaiense Lu and Skeletonema costatum(Grev.) Cleve in mesocosm experiments 被引量:10
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作者 ZHU Mingyuan xu zongjun +2 位作者 LI Ruixiang WANG Zongling SHI Xiaoyong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2009年第1期72-82,共11页
Three mesocosm experiments were conducted in the East China Sea during blooms of Prorocentrum donghaiense Lu in May of 1998,2002 and 2003 to examine the role of nutrients in the formation of these harmful algae blooms... Three mesocosm experiments were conducted in the East China Sea during blooms of Prorocentrum donghaiense Lu in May of 1998,2002 and 2003 to examine the role of nutrients in the formation of these harmful algae blooms(HABs).The results showed that there was interspecies competition between P.donghaiense and Skeletonema costatum(Grev.) Cleve.The cell number of P.donghaiense and S.costatum accounted for about 85% and 11% of the total cell number of phytoplankton respectively at the beginning of the experiment in May 1998.In May 2002,at the beginning of the experiment,the cell number of P.donghaiense accounted for 55%-66% of the total and S.costatum accounted for 32%-42%.The density of P.donghaiense and S.costatum was over 95% and 1%-5% respectively in May 2003.The results of these three mesocosm experiments showed that the dominant species in this specific community varied with different nutrient availability.Under low-phosphate conditions,the dominant species was P.donghaiense,while S.costatum became dominant in phosphate-replete cases.The average growth rate(1.08 d-1) of S.costatum in exponential growth phase was higher than that(0.39 d-1) of P.donghaiense.In the mesocosm,S.costatum predominated in the mesocosm by its rapid growth.When phosphorus was depleted,the cell number of S.costatum dropped rapidly,while P.donghaiense Lu decreased more slowly.The results from mesocosm experiment may explain why:(1) P.donghaiense bloom usually occurs in May,when phosphate concentration is low;(2) the bloom of S.costatum appears in early spring and summer time,when nutrients increase with increasing Changjiang River(Yangtze River) runoff;(3) the bloom of S.costatum is short and that of P.donghaiense Lu can last more than a month in the East China Sea. 展开更多
关键词 HABs Fasl China Sea mesocosm experiment interspeeies eompetition
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TEMPERATURE FIELD CONTROL PRINCIPLE AND CONTROL MODELS FOR CRYOGENIC MACHINING SYSTEM
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作者 Liu Fei Kang Geweng +1 位作者 xu zongjun Chongqing University 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 1995年第3期222-227,共17页
Cryogenic machining is a new technology which makes use of the special mechanicalprcperties of niaterials in a cryogenic or a 'super cold' state for machining. The control of acryogenic machining system(CMS)i... Cryogenic machining is a new technology which makes use of the special mechanicalprcperties of niaterials in a cryogenic or a 'super cold' state for machining. The control of acryogenic machining system(CMS)is one of the key problems which need to be solved for practi-cal cryogenic machining A temperature field control principle is presented which calculates theheat source temperature in the light of the tool temperature field information, and a control mod-el of three-dimensional dynamic temperature field for CMS is established, and the boundary con-ditions and the heat source of temperature field in orthogonal cutting are discussed. Based on theinvestigation of the control feature and technique. a prototype system for controlling CMS is crea-ted. 展开更多
关键词 Cryogenic-machining Temperature field Control model Control system
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