The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylam...The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylammonio]-1-propane-sulphonate (CHAPS)can enhance the dissolution of BBMV, and phosphatidylinositol-specific phosopholipase C(PI-PLC) can cleave the APN from midgut membrane. The APN was primarily purified usinga Mono-Q column. The results of immunoblotting showed that the 120 and 170kDa proteinsin the BBMV could bind Cry1Ac, and 120kDa APN was a glycosylphosphalidylinositol(GPI)-anchored protein. Two Bt-resistant strains (Bt-P, Bt-M) were obtained after beingselected for more than five years in laboratory using Bt insecticides and Bt transgeniccotton incorporated into diet separately. The resistance of Bt-P and Bt-M were 1083.3and 48.7 times that of susceptible strain. The genes encoding APN1 in midgut ofsusceptible and resistant H.armigera were cloned by PCR and RACE techniques. Theinferred amino acid sequences of APN1 possessed the common character of APN family ininsects. In comparison with APN1 in susceptible strain, three nucleotide mutations wereobserved in the APN1 of Bt-M strain and resulted in two amino acid replace in theputative protein sequences, and eight nucleotide mutations were observed in Bt-P strainand resulted in five amino acid replace.展开更多
The probability of 5He particle emission has been affirmed theoretically [J.S. Zhang, Science in China G47 (2004) 137]. In order to describe the 5He emission, the theoretical formula of the double-differential cross s...The probability of 5He particle emission has been affirmed theoretically [J.S. Zhang, Science in China G47 (2004) 137]. In order to describe the 5He emission, the theoretical formula of the double-differential cross section of emitted 5He is to be established. Based on the pick-up mechanism, used for calculating the formula of d, t, 3He, α emissions, the theoretical formula of double-differential cross section of 5 He is obtained, which is expressed in the form of Legendre coefficients. In the case of low incident energies, the configuration [J.S. Zhang, Science in China G47 (2004)137; J.S. Zhang, Commun. Theor. Phys. (Beijing, China) 39 (2003) 83] is the dominant part in the reaction processes.The calculated result indicates that the forward peaked angular distribution of the composite particle emission is weaker than that of the emitted single nucleon due to pick-up nucleon from the Fermi sea. As an example, the reactions of n + 14N have been calculated, and the Legendre coefficients of d, t, 3He, α, 5He emissions are obtained respectively.The results show that the forward tendency is decided by the average momentum per nucleon in the emitted composite particles. The larger the average momentum is, the stronger the forward tendency is.展开更多
基金Research was supported by the National Natural Science Fundation of China(30200182)973 Program of Ministry of Science and Techno1ogy,China(001CB109004).
文摘The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylammonio]-1-propane-sulphonate (CHAPS)can enhance the dissolution of BBMV, and phosphatidylinositol-specific phosopholipase C(PI-PLC) can cleave the APN from midgut membrane. The APN was primarily purified usinga Mono-Q column. The results of immunoblotting showed that the 120 and 170kDa proteinsin the BBMV could bind Cry1Ac, and 120kDa APN was a glycosylphosphalidylinositol(GPI)-anchored protein. Two Bt-resistant strains (Bt-P, Bt-M) were obtained after beingselected for more than five years in laboratory using Bt insecticides and Bt transgeniccotton incorporated into diet separately. The resistance of Bt-P and Bt-M were 1083.3and 48.7 times that of susceptible strain. The genes encoding APN1 in midgut ofsusceptible and resistant H.armigera were cloned by PCR and RACE techniques. Theinferred amino acid sequences of APN1 possessed the common character of APN family ininsects. In comparison with APN1 in susceptible strain, three nucleotide mutations wereobserved in the APN1 of Bt-M strain and resulted in two amino acid replace in theputative protein sequences, and eight nucleotide mutations were observed in Bt-P strainand resulted in five amino acid replace.
文摘The probability of 5He particle emission has been affirmed theoretically [J.S. Zhang, Science in China G47 (2004) 137]. In order to describe the 5He emission, the theoretical formula of the double-differential cross section of emitted 5He is to be established. Based on the pick-up mechanism, used for calculating the formula of d, t, 3He, α emissions, the theoretical formula of double-differential cross section of 5 He is obtained, which is expressed in the form of Legendre coefficients. In the case of low incident energies, the configuration [J.S. Zhang, Science in China G47 (2004)137; J.S. Zhang, Commun. Theor. Phys. (Beijing, China) 39 (2003) 83] is the dominant part in the reaction processes.The calculated result indicates that the forward peaked angular distribution of the composite particle emission is weaker than that of the emitted single nucleon due to pick-up nucleon from the Fermi sea. As an example, the reactions of n + 14N have been calculated, and the Legendre coefficients of d, t, 3He, α, 5He emissions are obtained respectively.The results show that the forward tendency is decided by the average momentum per nucleon in the emitted composite particles. The larger the average momentum is, the stronger the forward tendency is.
