CWMV-CP1 target gene and bar selection gene were co-transferred into commercial wheat variety of Yangmai158 by particle bombardment. In total, 145 resistant plants to 3 -5 mg L-1 Bialaphos were obtained, 21 plants wer...CWMV-CP1 target gene and bar selection gene were co-transferred into commercial wheat variety of Yangmai158 by particle bombardment. In total, 145 resistant plants to 3 -5 mg L-1 Bialaphos were obtained, 21 plants were identified to be positive in T0 generation by PCR-Southern test, and the transformation frequency had 0.99%. T1 plants were further tested by PCR and Southern hybridization. Results demonstrated that the alien resistance gene had been integrated into the wheat genome. The segregation ratio of CP1+ to CP1- in T1 generation was 1.0 to 1. 3, and didn't agree with Mendelian rule. RT-PCR result from T2 plants showed that the alien gene CWMV-CP1 had stable expression in wheat genetic background.展开更多
The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Resu...The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Results of histochemical stain assay showed that the Gus activity increased markedly during the recovery phase(21℃)after heat shock treatment at 42℃ for 2 h. Results of quantitative fluorometric assays indicated that maximal Gus activity after heat shock treatment could reach 5-6 times higher than that before heat shock treatment in transgenic lines HG6 and HG9. Based on several advantages of the heat shock promoter such as easy to induce, sensitivity to heat shock and high inductive activity, it can be potentially used to control expression of gene of interest in transgenic plants.展开更多
基金Thig study was supported by Europe Confederation International Cooperation with Developing Countries(ECINCO)Program(IC18-CT96-0049)Chinese“863”Program(2001-AA212111).
文摘CWMV-CP1 target gene and bar selection gene were co-transferred into commercial wheat variety of Yangmai158 by particle bombardment. In total, 145 resistant plants to 3 -5 mg L-1 Bialaphos were obtained, 21 plants were identified to be positive in T0 generation by PCR-Southern test, and the transformation frequency had 0.99%. T1 plants were further tested by PCR and Southern hybridization. Results demonstrated that the alien resistance gene had been integrated into the wheat genome. The segregation ratio of CP1+ to CP1- in T1 generation was 1.0 to 1. 3, and didn't agree with Mendelian rule. RT-PCR result from T2 plants showed that the alien gene CWMV-CP1 had stable expression in wheat genetic background.
基金supported by the National High Tech R&D Program of China(863 Program,2002AA2244011)the National Special Program for Research and Industrialization of Ttansgenic Plants,China(J2000-B-021).
文摘The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Results of histochemical stain assay showed that the Gus activity increased markedly during the recovery phase(21℃)after heat shock treatment at 42℃ for 2 h. Results of quantitative fluorometric assays indicated that maximal Gus activity after heat shock treatment could reach 5-6 times higher than that before heat shock treatment in transgenic lines HG6 and HG9. Based on several advantages of the heat shock promoter such as easy to induce, sensitivity to heat shock and high inductive activity, it can be potentially used to control expression of gene of interest in transgenic plants.
