Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus(AcMNPV),and hence can serve as an effective test vehicle to understand the AcMNPV infectio...Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus(AcMNPV),and hence can serve as an effective test vehicle to understand the AcMNPV infection mechanism.In this study,through high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection,3463 pieces of time-series differentially expressed RNA(1,200 mRNA and 2,263 lncRNA)are identified and justified by experimental verification of randomly selected samples from them,proving the validity of the bioinformatical analysis on this topic.Functional enrichment analysis and target prediction are performed on those differentially expressed RNA,from which the major functional enrichment distribution of those differentially expressed mRNA is derived.It has been found that the differential genes are mainly in the cellular anatomical entity and intracellular in terms of the cellular component,and in the binding and catalytic activity in terms of the molecular function.Also,the differential mRNA are mainly concentrated in global and overview maps,signal transduction,infectious diseases,and viral,etc.Moreover,those mRNA targeted by lncRNA are predicted.The correlation between those differentially expressed lncRNA and mRNA indicates that lncRNA is very likely playing an important role in the interaction between virus and host.Aided by an advanced co-expression analysis approach,the“hub”RNA is also identified.The study in this work pave the way for further analyzing and understanding how AcMNPV escapes from the host’s immunity,manipulates the host to realize the selfmultiplication,and realizes the timely conversion between its two particle forms,laying the foundation for uncovering the host’s immune response process.展开更多
The feasibility and safety of intracardiac echocardiography(ICE)-guided catheter ablation for atrial fibrillation(AF)using a minimal/zero-fluoroscopy approach have recently been reported.This approach helps to reduce ...The feasibility and safety of intracardiac echocardiography(ICE)-guided catheter ablation for atrial fibrillation(AF)using a minimal/zero-fluoroscopy approach have recently been reported.This approach helps to reduce ionizing radiation exposure and orthopedic complications resulting from using lead aprons.The objectives of this planned prospective,multicenter randomized controlled trial(RCT)(paroxysmal AF(PAF)-ICE trial;ChiCTR2000033624)are to evaluate the efficacy and safety of ICE-guided minimal-fluoroscopy ablation in patients with PAF and the impact on occupational hazards among lab staff.Patients will be randomized in a 1:1 ratio to 2 groups:minimal fluoroscopy group(n=216)and traditional approach group(n=216).In the minimal fluoroscopy group,an ICE catheter will be used for geometry/anatomic construction,transseptal puncture,catheter tracking,and effusion monitoring.Pulmonary vein isolation(PVI)will be performed using an open-irrigated radiofrequency SmartTouch Surround Flow or SmartTouch catheter(Biosense Webster,Diamond Bar,California,USA),and confirmed by a multipolar Lasso or PentaRay catheter(Biosense Webster).In the traditional approach group,an ICE catheter will not be used.Transseptal puncture will be performed under fluoroscopic guidance,with all geometries constructed by mapping the catheters.The primary efficacy endpoint is freedom from AF recurrence(without antiarrhythmic medications)at 12months after ablation.Other endpoints include duration of lead apron use,measures of intra-procedural efficiency,and peri-procedural complications.This RCT will evaluate the efficacy and safety of ICE-guided minimal-fluoroscopy ablation in patients with PAF,also evaluate the benefits to lab staff(regarding reducing occupational hazards)related to this“minimal/zero-fluoroscopy”and“leadless”mode.展开更多
文摘Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus(AcMNPV),and hence can serve as an effective test vehicle to understand the AcMNPV infection mechanism.In this study,through high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection,3463 pieces of time-series differentially expressed RNA(1,200 mRNA and 2,263 lncRNA)are identified and justified by experimental verification of randomly selected samples from them,proving the validity of the bioinformatical analysis on this topic.Functional enrichment analysis and target prediction are performed on those differentially expressed RNA,from which the major functional enrichment distribution of those differentially expressed mRNA is derived.It has been found that the differential genes are mainly in the cellular anatomical entity and intracellular in terms of the cellular component,and in the binding and catalytic activity in terms of the molecular function.Also,the differential mRNA are mainly concentrated in global and overview maps,signal transduction,infectious diseases,and viral,etc.Moreover,those mRNA targeted by lncRNA are predicted.The correlation between those differentially expressed lncRNA and mRNA indicates that lncRNA is very likely playing an important role in the interaction between virus and host.Aided by an advanced co-expression analysis approach,the“hub”RNA is also identified.The study in this work pave the way for further analyzing and understanding how AcMNPV escapes from the host’s immunity,manipulates the host to realize the selfmultiplication,and realizes the timely conversion between its two particle forms,laying the foundation for uncovering the host’s immune response process.
基金supported by the Investigator-Initiated Study Program of Biosense Webster,Inc.(Diamond Bar,California,USA).
文摘The feasibility and safety of intracardiac echocardiography(ICE)-guided catheter ablation for atrial fibrillation(AF)using a minimal/zero-fluoroscopy approach have recently been reported.This approach helps to reduce ionizing radiation exposure and orthopedic complications resulting from using lead aprons.The objectives of this planned prospective,multicenter randomized controlled trial(RCT)(paroxysmal AF(PAF)-ICE trial;ChiCTR2000033624)are to evaluate the efficacy and safety of ICE-guided minimal-fluoroscopy ablation in patients with PAF and the impact on occupational hazards among lab staff.Patients will be randomized in a 1:1 ratio to 2 groups:minimal fluoroscopy group(n=216)and traditional approach group(n=216).In the minimal fluoroscopy group,an ICE catheter will be used for geometry/anatomic construction,transseptal puncture,catheter tracking,and effusion monitoring.Pulmonary vein isolation(PVI)will be performed using an open-irrigated radiofrequency SmartTouch Surround Flow or SmartTouch catheter(Biosense Webster,Diamond Bar,California,USA),and confirmed by a multipolar Lasso or PentaRay catheter(Biosense Webster).In the traditional approach group,an ICE catheter will not be used.Transseptal puncture will be performed under fluoroscopic guidance,with all geometries constructed by mapping the catheters.The primary efficacy endpoint is freedom from AF recurrence(without antiarrhythmic medications)at 12months after ablation.Other endpoints include duration of lead apron use,measures of intra-procedural efficiency,and peri-procedural complications.This RCT will evaluate the efficacy and safety of ICE-guided minimal-fluoroscopy ablation in patients with PAF,also evaluate the benefits to lab staff(regarding reducing occupational hazards)related to this“minimal/zero-fluoroscopy”and“leadless”mode.
