There are mainly four kinds of models to record and deal with historical information. By taking them as reference, the spatio-temporal model based on event semantics is proposed. In this model, according to the way fo...There are mainly four kinds of models to record and deal with historical information. By taking them as reference, the spatio-temporal model based on event semantics is proposed. In this model, according to the way for describing an event, all the information are divided into five domains. This paper describes the model by using the land parcel change in the cadastral information system, and expounds the model by using five tables corresponding to the five domains. With the aid of this model, seven examples are given on historical query, trace back and recurrence. This model can be implemented either in the extended relational database or in the object-oriented database.展开更多
Previously, we isolated a vernalization-related gene, VER2, from winter wheat (Triticum aestivum L.) and its expression was restricted in the immature leaves of vernal-ized wheat seedlings. To further investigate the ...Previously, we isolated a vernalization-related gene, VER2, from winter wheat (Triticum aestivum L.) and its expression was restricted in the immature leaves of vernal-ized wheat seedlings. To further investigate the regulation of VER2 expression and the function of its promoter, we iso-lated a 41.7 kb genomic clone containing VER2 gene from a transformation-competent artificial chromosome (TAC) li-brary of wheat (Triticum aestivum-Haynaldia villosa). The sequence analysis showed that there were eleven predicted genes in the TAC. The exons of gene 3 corresponded to the cDNA sequence of VER2 gene. Analysis of VER2 promoter structure showed that there were three small repeat se-quences divided by two large repeat sequences. The putative response elements, such as abscisic acid response elements (ABRE), MeJA-response elements (Me-JARE), low-tempe- rature response elements (LTR), endosperm expression ele-ments, MYB binding sites and similar elements to GA re-sponse elements (GARE), were involved in the VER2 pro-moter region. Construct containing the VER2 promoter (-5895 to +73) driving GFP reporter gene was bombarded into vernalized or non-vernalized immature leaves in wheat. The vernalized immature leaves showed bright green fluo-rescence after incubation for 24 h, however, the green fluo-rescence was not observed in the non-vernalization leaves under the same condition. These results suggested that vernalization was essential for the function of VER2 promoter in the immature leaves of winter wheat.展开更多
文摘There are mainly four kinds of models to record and deal with historical information. By taking them as reference, the spatio-temporal model based on event semantics is proposed. In this model, according to the way for describing an event, all the information are divided into five domains. This paper describes the model by using the land parcel change in the cadastral information system, and expounds the model by using five tables corresponding to the five domains. With the aid of this model, seven examples are given on historical query, trace back and recurrence. This model can be implemented either in the extended relational database or in the object-oriented database.
文摘Previously, we isolated a vernalization-related gene, VER2, from winter wheat (Triticum aestivum L.) and its expression was restricted in the immature leaves of vernal-ized wheat seedlings. To further investigate the regulation of VER2 expression and the function of its promoter, we iso-lated a 41.7 kb genomic clone containing VER2 gene from a transformation-competent artificial chromosome (TAC) li-brary of wheat (Triticum aestivum-Haynaldia villosa). The sequence analysis showed that there were eleven predicted genes in the TAC. The exons of gene 3 corresponded to the cDNA sequence of VER2 gene. Analysis of VER2 promoter structure showed that there were three small repeat se-quences divided by two large repeat sequences. The putative response elements, such as abscisic acid response elements (ABRE), MeJA-response elements (Me-JARE), low-tempe- rature response elements (LTR), endosperm expression ele-ments, MYB binding sites and similar elements to GA re-sponse elements (GARE), were involved in the VER2 pro-moter region. Construct containing the VER2 promoter (-5895 to +73) driving GFP reporter gene was bombarded into vernalized or non-vernalized immature leaves in wheat. The vernalized immature leaves showed bright green fluo-rescence after incubation for 24 h, however, the green fluo-rescence was not observed in the non-vernalization leaves under the same condition. These results suggested that vernalization was essential for the function of VER2 promoter in the immature leaves of winter wheat.
