根本原因分析法对糖尿病肾病血液透析患者不良事件、负性情绪及满意度的影响

目的观察根本原因分析法对糖尿病肾病血液透析患者不良事件、负性情绪及满意度的影响。方法回顾性选取2021年1月—2023年7月于山东国欣颐养集团枣庄中心医院进行血液透析治疗的76例糖尿病肾病患者的临床资料,按照护理方式的不同分为观察组和对照组,各38例。对照组实施常规护理,观察组在对照组的基础上实施根本原因分析法。比较两组不良事件发生率、负性情绪评分(焦虑自评量表、抑郁自评量表)及护理满意度。结果干预后,观察组不良事件发生率为2.63%(1/38),低于对照组的18.42%(7/38),差异有统计学意义(χ^(2)=4.537,P<0.05)。干预后,观察组抑郁、焦虑评分均低于对照组,差异有统计学意义(P均<0.05)。观察组满意度高于对照组,差异有统计学意义(P<0.05)。结论根本原因分析法干预可降低不良事件发生率,改善患者不良情绪,提高其护理满意度。

FPGA based hardware platform for trapped-ion-based multi-level quantum systems

We report a design and implementation of a field-programmable-gate-arrays(FPGA)based hardware platform,which is used to realize control and signal readout of trapped-ion-based multi-level quantum systems.This platform integrates a four-channel 2.8 Gsps@14 bits arbitrary waveform generator,a 16-channel 1 Gsps@14 bits direct-digital-synthesisbased radio-frequency generator,a 16-channel 8 ns resolution pulse generator,a 10-channel 16 bits digital-to-analogconverter module,and a 2-channel proportion integration differentiation controller.The hardware platform can be applied in the trapped-ion-based multi-level quantum systems,enabling quantum control of multi-level quantum system and highdimensional quantum simulation.The platform is scalable and more channels for control and signal readout can be implemented by utilizing more parallel duplications of the hardware.The hardware platform also has a bright future to be applied in scaled trapped-ion-based quantum systems.

口腔护士参与北京冬奥会闭环管理下医疗保障工作体验的质性研究

目的了解口腔护士参与北京冬奥会闭环管理下医疗保障过程中的经历和感受。方法采用质性研究方法,于2022年3月对11名口腔护士进行半结构式访谈,并采用Colaizzi 7步分析法进行资料分析、归纳、总结。结果共提炼出5个主题:口腔护士综合专业实践技能不足;口腔护士在二级防护下缺乏工作经验;闭环管理中充满焦虑和紧张;收获来自家庭和社会的支持和帮助;提升认知,满足自我实现的需求。结论提示护理管理者需加强口腔综合型护理人才的培养和建设;完善模拟疫情下的演练;关注负面情绪,及时采取心理疏导;重视护士工作-家庭冲突,倡导社会支持系统,满足职业获益感。

内蒙古兴和县低山丘陵立地类型划分与林草适宜性评价

为了解半干旱区宜林荒山合理利用方式,于2016年7月和8月对兴和县低山丘陵造林地进行立地类型划分与林草适宜性评价。通过对38个样地的油松Pinus tabulaeformis树高和草本植物地上部分生物量进行多元线性回归分析,结果表明:影响油松生长的主导因子包括土壤厚度和平均土壤含水率,影响草本生物量的主导因子包括表土含水率、表土全氮质量分数、表土全磷质量分数和土壤砾石含量。通过单因素方差分析,选取和验证了地貌、地形、坡向、土壤质地、土层厚度、砾石含量作为立地划分因子,最终将研究区荒山荒坡造林地划分出8种立地类型。林草适宜性评价结果表明:第1级的立地同时适宜油松和草本植物的生长;第2级的立地较适宜油松生长,但不适宜草本植物生长;第3级的立地较为适宜草本植物生长,油松生长状况略差;第4级的立地不适宜油松生长,但草本植物生长稍好。根据各立地类型情况提出相应的林草措施建议。

基于抗差岭估计的相机标定方法

数字工业摄影测量中,十参数模型是目前应用最广泛的相机畸变模型,但也存在过度参数化问题。相机标定以像点坐标为观测值,当标定模型同时受到复共线和观测异常的影响时,相机标定结果也会受到影响。为提高相机标定精度,提出基于抗差岭估计的相机标定方法,该方法不仅可以改善模型中由于过度参数化导致的复共线问题,而且可以有效抵制观测值异常的影响,提高相机参数求解精度。

针灸对阿尔茨海默病大鼠的治疗作用及相关信号通路研究

目的 研究针灸对阿尔茨海默病大鼠的治疗作用及相关信号通路。方法 取40只SD大鼠,随机分为对照组(正常大鼠,不处理)、假手术组(大鼠双侧海马CA1区注射等量生理盐水)、模型组(建立阿尔茨海默病模型)、干预组(建立阿尔茨海默病模型+针灸),每组各10只。取各组大鼠脑组织,经苏木精-伊红染色行病理学检查;比较各组大鼠脑皮层区Janus激酶2(JAK2)、信号转导子和转录激活子3(STAT3)和炎性细胞因子白细胞介素1 (IL-1)、白细胞介素6(IL-6)阳性细胞数比率;采用免疫印迹法检测大鼠脑皮层区JAK2和STAT3蛋白表达情况。结果 病理学检查提示,模型组和干预组大鼠均出现不同程度的病理改变,且干预组的病理损伤程度明显轻于模型组。模型组、干预组大鼠脑皮层区JAK2、STAT3、IL-1和IL-6阳性细胞数比率较对照组、假手术组均明显升高(P <0. 01);干预组大鼠脑皮层区JAK2、STAT3、IL-1和IL-6阳性细胞数比率较模型组明显下降(P <0. 01)。免疫印迹法检测可知,相比对照组、假手术组,模型组、干预组大鼠脑皮层区JAK2和STAT3蛋白表达水平均明显升高(P <0. 01);相比模型组,干预组大鼠脑皮层区JAK2和STAT3蛋白表达水平均明显下降(P <0. 01)。结论 针灸治疗可有效减轻阿尔茨海默病大鼠脑损伤,其原因可能在于针灸治疗可下调JAK2和STAT3蛋白表达,阻滞JAK2/STAT3信号通路的异常活化,抑制炎性细胞因子的表达,从而可抑制慢性炎症反应的进展,减轻病情程度,起到良好的治疗作用。

Ph染色体阴性骨髓增殖性肿瘤的治疗进展

Ph染色体阴性骨髓增殖性肿瘤(MPN)是一组起源于多能造血干细胞的恶性骨髓增殖性疾病,Janus激酶(JAK)-信号转导及转录激活因子信号转导通路等多种信号通路的异常活化可能参与MPN的发病机制,并与不同临床表型相关。近年来,针对不同信号通路的新型药物(如JAK抑制剂、鼠双微体2抑制剂、组蛋白脱乙酰酶抑制剂)的临床研究成为MPN治疗的新方向,弥补了传统药物缺乏基于发病机制治疗的不足,使提高MPN疗效、降低突变基因负荷、逆转纤维化成为可能。未来,新型药物的不断涌现有望为MPN的治疗带来新的选择。

Expression of serum Dickkopf-1 in gastric cancer patients

Objective:To explore the expression and clinical significance of DKK-1 protein in patients with gastric cancers.Methods:Enzyme linked imtnuno sorbent assay was used to detect expressions of serum DKK-1 protein in 90 cases of gastric cancers,50 cases of gastric benign disease and 40 healthy cases.The dynamic change in serum DKK-1 protein of gastric cancer patients who accepted radical operation for a month was also observed.Results:The expression of serum DKK-1 protein in gastric cancer groups was significantly higher than that in gastric benign group's(P<0.01) and in health control(P<0.01).Serum DKK 1 level was increased gradually along with the progress of the disease.Serum DKK 1 levels were significantly higher in patients at TNM staging Ⅲ and Ⅳ than patients at TNM staging Ⅰ andⅡ.Level of serum DKK-1 was related to microvascular invasion,differentiation degree and infiltration depth.Level of serum DKK-1 was significantly reduced in patients after radical surgery(P<0.01).Conclusions:The expression of serum DKK-1 protein in gastric cancer patients is increased.Level of serum DKK-1 is related to TNM staging,microvascular invasion,differentiation degree and infiltration depth.DKK-1 detection can be used as a reference index in monitoring gastric cancer progress and biological behavior.

Readout electronics of a prototype spectrometer for measuring low-energy ions in solar wind plasma

Readout electronics is developed for a prototype spectrometer for in situ measurement of low-energy ions of30 e V/e–20 ke V/e in the solar wind plasma.A low-noise preamplifier/discriminator(A111F) is employed for each channel to process the signal from micro-channel plate(MCP) detectors.A high-voltage(HV) supply solution based on a HV module and a HV optocoupler is adopted to generate a fast sweeping HV and a fixed HV.Due to limitation of telemetry bandwidth in space communication,an algorithm is implemented in an FPGA(field programmable gate array) to compress the raw data.Test results show that the electronics achieves a 1 MHz event rate and a large input dynamic range of 95 p C.A slew rate of 0.8 V/ls and an integral nonlinearity of 0.7-LSB for the sweeping HV,and a precision of less than 0.8 % for the fixed HV are obtained.A vacuum beam test shows an energy resolution of 12 ± 0.7 % full width at half maximum(FWHM) is achieved,and noise counts are less than10/sec,indicating that the performance meets the physical requirement.

Cloning,expression,identification and bioinformatics analysis of Rv3265c gene from Mycobacterium tuberculosis in Escherichia coli

Objective:To clone and express Rv3265c gene of Mycobacterium tuberculosis in Escherichia coli (E.coli) under optimistic conditions,obtain and identify protein expressed,analyze the structure and characteristics of the protein using bioinformatics methods for future applications.Methods: Rv3265c gene from Mycobacterium tuberculosis H37Rv was amplified by polymerase chain reaction,and was cloned into the pET-30a vector after purification and recovery.The recombinant plasmid was sequenced and expressed in E.coli BL21(DE3),and then purified and identified by western blotting.The essential physical-chemical properties of the protein were predicated by bioinformatics tools,including subcellular location,secondary structure,domains,antigenic epitopes,etc.Tertiary structure of the protein based on homology modeling was estabUshed,while multi-sequence homological alignment and phylogenetic analysis were preformed.Results:The recombinant protein was obtained in soluble fraction from expression system in E.coli B121(DE3) carrying pET30- Rv3265c plasmid,and Rv3265c gene was expressed correctly.Bioinformatics analysis showed the protein contained no signal peptide and transmembrane helices,located outside of membrane.Secondary structure analysis revealed it containedα-helix,extended strand and random coil,46.8%,14.6%,38.6%,respectively.Furthermore,it possessed six potential antigenic epitopes,one glycosyl transferase domain.A simple three-dimensional model of this protein was constructed by Swiss-model sever.Both sequences and structures were conservative and especial either in gene or in protein.Conclusions:Rv3265c gene might be a desirable molecular target for anti-tuberculosis drug and vaccine.The purified protein from expression will be utilized to study the kinetics of L-rhamnosyltransferase and to develope an enzyme assay for screening vaccine or drug.

省级新一轮林地保护利用规划编制探讨——以贵州省为例

总结了林地保护利用规划特点。结合贵州省新一轮林保规划编制,分析了国土空间规划体系下林地界定标准衔接、基础数据源融合及规划发展蓝图定位等问题。为衔接协同国土空间规划,提出了新一轮省级林地保护利用规划用地分类协同衔接、联动校核科学整合及传导指引空间布局等机制策略。

A high performance fast-Fourier-transform spectrum analyzer for measuring spin noise spectrums

A high performance fast-Fourier-transform (FFT) spectrum analyzer, which is developed for measure spin noise spectrums, is presented in this paper. The analyzer is implemented with a field-programmable-gate-arrays (FPGA) chip for data and command management. An analog-to-digital-convertor chip is integrated for analog signal acquisition. In order to meet the various requirements of measuring different types of spin noise spectrums, multiple operating modes are designed and realized using the reprogrammable FPGA logic resources. The FFT function is fully managed by the programmable resource inside the FPGA chip. A 1 GSa/s sampling rate and a 100 percent data coverage ratio with non-dead-time are obtained. 30534 FFT spectrums can be acquired per second, and the spectrums can be on-board accumulated and averaged. Digital filters, multi-stage reconfigurable data reconstruction modules, and frequency down conversion modules are also implemented in the FPGA to provide flexible real-time data processing capacity, thus the noise floor and signals aliasing can be suppressed effectively. An efficiency comparison between the FPGA-based FFT spectrum analyzer and the software-based FFT is demonstrated, and the high performance FFT spectrum analyzer has a significant advantage in obtaining high resolution spin noise spectrums with enhanced efficiency.

中医治疗痤疮(肺风粉刺)的进展分析

痤疮(肺风粉刺)近年来发病率较高,严重影响着患者的身心健康。本文从中医学角度简要综述了其病机理论和治疗方法两方面的研究进展,以期为临床工作提供更多依据。

相对论黏滞流体动力学演化

基于相对论流体动力学,推导出了黏滞夸克-胶子等离子体的流体动力学演化方程。在大型强子对撞机能标下得到了系统温度随时间和径向半径的分布关系,发现温度对径向半径的依赖十分微弱。数值计算结果表明,横向流效应会加快夸克-胶子等离子的冷却速度,同时降低夸克-胶子等离子体的寿命。

Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon a2b

Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products.

Magnetic Phase Transition in Two-Dimensional CrBr_(3) Probed by a Quantum Sensor

Recently,magnetism in two-dimensional(2 D)van der Waals(vd W)materials has attracted wide interests.It is anticipated that these materials will stimulate discovery of new physical phenomena and novel applications.The capability to quantitatively measure the magnetism of 2 D magnetic vd W materials is essential to understand these materials.Here we report on quantitative measurements of ferromagnetic-to-paramagnetic phase transition of an atomically thin(down to 11 nm)vd W magnet,namely Cr Br_(3),with a Curie point of 37.5 K.This experiment demonstrates that surface magnetism can be quantitatively investigated,which is useful for a wide variety of potential applications.

Experimental Protection of the Spin Coherence of a Molecular Qubit Exceeding a Millisecond

Molecular qubits are promising as they can benefit from tailoring and versatile design of chemistry.It is essential to reduce the decoherence of molecular qubits caused by their interactions with the environment.Herein the dynamical decoupling(DD)technique is utilized to combat such decoherence.The coherence time for a transitionmetal complex(PPh_(4))_(2)[Cu(mnt)_(2)]is prolonged from 6.8μs to 1.4 ms.The ratio of the coherence time and the length ofπ/2 pulse,defined as the single qubit figure of merit(QM),reaches 1.4×10^(5),which is 40 times greater than what previously reported for this molecule.Our results show that molecular qubits,with milliseconds coherence time,are promising candidates for quantum information processing.

Expression level and clinical significance of KLK7 and E-CAD in gastric cancer

Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.

RNA m^(6)A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progression

As the most common internal modification of mRNA,Ne-methyladenosine(m^(6)A)and its regulators modulate gene expression and play critical roles in various biological and patholog-ical processes including tumorigenesis.It was reported previously that m^(6)A methyltransferase(writer),methyltransferase-like 3(METTL3)adds m^(6)A in primary microRNAs(pri-miRNAs)and fa-cilitates its processing into precursor miRNAs(pre-miRNAs).However,it is unknown whether m^(6)A modification also plays a role in the maturation process of pre-miRNAs and(if so)whether such a function contributes to tumorigenesis.Here,we found that YTHDF2 is aberrantly overexpressed in acute myeloid leukemia(AML)patients,especially in relapsed patients,and plays an onco-genic role in AML.Moreover,YTHDF2 promotes expression of miR-126-3p(also known as miR-126,as it is the main product of precursor miR-126(pre-miR-126)),a miRNA that was reported as an oncomiRNA in AML,through facilitating the processing of pre-miR-126 into mature miR-126.Mechanistically,YTHDF2 recognizes m^(6)A modification in pre-miR-126 and recruits AGO2,a regulator of pre-miRNA processing,to promote the maturation of pre-miR-126.YTHDF2 posi-tively and negatively correlates with miR-126 and miR-126's downstream target genes,respec-tively,in AML patients,and forced expression of miR-126 could largely rescue YTHDF2/Ythdf2 depletion-mediated suppression on AML cell growth/proliferation and leukemogenesis,indi-cating that miR-126 is a functionally important target of YTHDF2 in AML.Overall,our studies not only reveal a previously unappreciated YTHDF2/miR-126 axis in AML and highlight the ther-apeutic potential of targeting this axis for AML treatment,but also suggest that m^(6)A plays a role in pre-miRNA processing that contributes to tumorigenesis.

Simultaneous Dehalogenation and Hydrogenation in Sonogashira Coupling

We herein report a simultaneous dehalogenation and hydrogenation(DHH)reaction in Sonogashira coupling involving hexahalogenobenzene C6X6(X=I,Br),in which a halogen on the aryl polyhalide substrate was substituted by a hydrogen atom.First,a steric bulky terminal alkyne 5 was designed and synthesized to study the influence of the reaction conditions(e.g.,catalyst,solvent,temperature)and the halogens on the substrates C6X6 on the DHH reaction.Moreover,based on the optimized conditions,a terminal alkyne 6 with less steric hindrance was further synthesized to investigate the influence of alkyne on the DHH reaction.As a result,two aromatic polyynes 7 and 8 were were further studied and compared.The influence produced by the alkynes and aryl polyhalides substrate provide insights into Sonogashira coupling involving terminal alkyne with huge steric hindrance and polyhalogenated aromatic hydrocarbons.