BACKGROUND Chronic renal failure(CRF) patients are predisposed to arrhythmias, while the detailed mechanisms are unclear. We hypothesized the chronic inflammatory state of CRF patients may lead to cardiac sympathetic ...BACKGROUND Chronic renal failure(CRF) patients are predisposed to arrhythmias, while the detailed mechanisms are unclear. We hypothesized the chronic inflammatory state of CRF patients may lead to cardiac sympathetic remodeling, increasing the incidence of ventricular arrhythmia(VA) and sudden cardiac death. And explored the role of atorvastatin and etanercept in this process.METHODS A total of 48 rats were randomly divided into sham operation group(Sham group), CRF group, CRF + atorvastatin group(CRF + statin group), and CRF + etanercept group(CRF + rhTNFR-Fcgroup). Sympathetic nerve remodeling was assessed by immunofluorescence of growth-associated protein 43(GAP-43) and tyrosine hydroxylase positive area fraction. Electrophysiological testing was performed to assess the incidence of VA by assessing the ventricular effective refractory period and ventricular fibrillation threshold. The levels of tumor necrosis factor-alpha(TNF-α) and interleukin-1beta were determined by Western blotting and enzyme-linked immunosorbent assay.RESULTS Echocardiogram showed that compared with the Sham group, left ventricular end-systolic diameter and ventricular weight/body weight ratio were significantly higher in the CRF group. Hematoxylin-eosin and Masson staining indicated that myocardial fibers were broken, disordered, and fibrotic in the CRF group. Western blotting, enzyme-linked immunosorbent assay,immunofluorescence and electrophysiological examination suggested that compared with the Sham group, GAP-43 and TNF-α proteins were significantly upregulated, GAP-43 and tyrosine hydroxylase positive nerve fiber area was increased, and ventricular fibrillation threshold was significantly decreased in the CRF group. The above effects were inhibited in the CRF + statin group and the CRF + rhTNFR-Fcgroup.CONCLUSIONS In CRF rats, TNF-α was upregulated, cardiac sympathetic remodeling was more severe, and the nephrogenic cardiac sympathetic remodeling existed. Atorvastatin and etanercept could downregulate the expression of TNF-α or inhibit its activity, thus inhibited the above effects, and reduced the occurrence of VA and sudden cardiac death.展开更多
Chalcone is a plant metabolite widely found in fruits,vegetables,spices and tea,and has anti-tumor,anti-inflammation,immunomodulation,antibacterial and anti-oxidation activities,as well as many other pharmacological a...Chalcone is a plant metabolite widely found in fruits,vegetables,spices and tea,and has anti-tumor,anti-inflammation,immunomodulation,antibacterial and anti-oxidation activities,as well as many other pharmacological and biological effects.Our team has shown that its analogs have antioxidant activity,and oxidative stress is a pathological hallmark of retinal ischemia/reperfusion injury that can lead to retinal damage and visual loss.This investigation aims to identify a chalcone that protects retinal ganglion cells in vitro from the effects of oxidative stress and examine its mechanism.Rat retinal ganglion cell-5 cells were pretreated with chalcones and then exposed to tert-butyl hydroperoxide that causes oxidative damage.Controls received dimethyl sulfoxide only or tert-butyl hydroperoxide in dimethyl sulfoxide.Only（E）-3,4-dihydroxy-2′-methylether ketone（L2 H17）,of the five chalcone analogs,markedly increased the survival rate of oxidatively injured RGC-5 cells.Thus,subsequent experiments only analyzed the results of the L2 H17 intervention.Cell viability and apoptosis were measured.Intracellular superoxide dismutase and reactive oxygen species levels were used to assess induced oxidative stress.The mechanism of action by L2 H17 was explored by measuring the ER stress/UPR pathway and the expression and localization of Nrf2.All results demonstrated that L2 H17 could reduce the apoptosis of oxidatively injured cells,inhibit caspase-3 activity,increase Bcl-2 expression,decrease Bad expression,increase the activity of superoxide dismutase,inhibit the production of reactive oxygen species,increase Nrf2 immunoreactivity,and reduce the activating transcription factor 4,phospho-eukaryotic initiation factor 2 and CHOP expression.L2 H17 protects retinal ganglion cells induced by oxidative stress by regulating Nrf2,which indicates that it has the potential to become a drug for retinal ischemia/reperfusion.展开更多
A kind of novel electroluminescence polymer has been prepared by oxidative-coupling polymerization of N-alkyl carbazole and trans-stilbene. The structure of the copolymers was characterized by elemental analysis, UV-v...A kind of novel electroluminescence polymer has been prepared by oxidative-coupling polymerization of N-alkyl carbazole and trans-stilbene. The structure of the copolymers was characterized by elemental analysis, UV-vis and FTIR spectra. The copolymers have good solubility in chloroform and acetone. Fluorescence spectra showed that these polymers are blue-light emitting materials.展开更多
Objective:To detect serum contents of IgE, Eotaxin and explore the relationship between serum IgE, Eotaxin and asthma severity.Methods: 140 cases of asthma children diagnosed in our hospital during January 2017 to Mar...Objective:To detect serum contents of IgE, Eotaxin and explore the relationship between serum IgE, Eotaxin and asthma severity.Methods: 140 cases of asthma children diagnosed in our hospital during January 2017 to March 2018 were chosen as Asthma group, 100 cases of healthy children vaccinated in our hospital at the same time were chosen as Normal control group. Serum contents of IgE, Eotaxin, inflammatory mediators and airway remodeling indexes were detected in two groups. Correlation analysis was used to evaluate the relationship between serum IgE, Eotaxin content and severity of asthma.Results: Serum contents of IgE and Eotaxin in Asthma group were significantly higher than those in Normal control group, and contents of IL-9, IL-17, IL-18 and IL-33 were higher than those in Normal control group, IL-35 was lower than that in Normal control group;serum contents of MMP-2, MMP-9, TGF-β and VEGF in Asthma group were significantly higher than those in Normal control group (P<0.05). Pearson test found that serum contents of IgE, Eotaxin in asthma children were positively correlated with inflammatory mediators such as IL-9, IL-17, IL-18, IL-33, negatively correlated with IL-35;positively correlated with airway remodeling indexes such as MMP-2, MMP-9, TGF-β and VEGF.Conclusion: Serum contents of IgE, Eotaxin in asthma children are significantly higher than those in normal children, and the specific contents were closely related to the degree of systemic inflammatory response and airway remodeling.展开更多
Objective:To investigate the efficacy of botulinum toxin type A combined with nanofat in the treatment of facial rejuvenation.Methods:from January 2020 to January 2021,20 patients with facial wrinkles in our hospital(...Objective:To investigate the efficacy of botulinum toxin type A combined with nanofat in the treatment of facial rejuvenation.Methods:from January 2020 to January 2021,20 patients with facial wrinkles in our hospital(The First Affiliated Hospital of Dalian Medical University)were treated with botulinum toxin type A combined with nanofat,and the effect,duration and complications were observed.Results:After 1,3 and 6 months of injection,the effective rates were 90%,75%and 40%,respectively;there was no occurrence of postoperative infection,fat mass,granuloma,and other adverse reactions.Conclusion:Botulinum toxin type A combined with nanofat filling has a clear therapeutic effect in the facial rejuvenation treatment,and can improve the skin texture while improving wrinkles,which is worthy of further popularization and application in clinic.展开更多
Background:Cytochrome b561(CYB561)plays a critical role in neuroendocrine function,cardiovascular regulation,and tumor growth;however,the prognostic value of CYB561 in patients with breast cancer and the relationship ...Background:Cytochrome b561(CYB561)plays a critical role in neuroendocrine function,cardiovascular regulation,and tumor growth;however,the prognostic value of CYB561 in patients with breast cancer and the relationship between CYB561 expression and immune infiltration in breast cancer remain unclear.Methods:The mRNA expression and clinical data of patients with breast cancer were obtained from The Cancer Genome Atlas database.Functional enrichment analysis was used to explore underlying biological functions associated with CYB561.The methylation status of CYB561 was analyzed using the MethSurv database.The enrichment score of immune cell infiltration for CYB561 in breast cancer was calculated using single-sample gene set enrichment analysis.The prognostic value of CYB561 was evaluated using Kaplan-Meier method and Cox regression analysis.Based on the results of the multivariate Cox analysis,a nomogram was constructed to predict the effect of CYB561 expression on overall survival(OS).Results:The results showed that CYB561 was highly expressed in breast cancer tissues.Hypomethylation of CYB561 is associated with an unfavorable prognosis.In multivariate Cox regression analysis,CYB561 was an independent prognostic factor for OS.Functional enrichment analysis indicated that estrogen signaling pathway,inflammatory response,KRAS signaling pathway,epithelial-mesenchymal transition,leukocyte migration,and regulation of lymphocyte activation were strongly enriched in the low CYB561 expression group.Additionally,CYB561 expression was negatively correlated with immune infiltration of B cells,plasmacytoid dendritic cells,dendritic cells,and neutrophils.Conclusion:CYB561 may serve as a potential biomarker for breast cancer diagnosis and prognosis.展开更多
In this work,we developed a simple and direct circuit model with a dual two-diode model that can be solved by a SPICE numerical simulation to comprehensively describe the monolithic perovskite/crystalline silicon(PVS/...In this work,we developed a simple and direct circuit model with a dual two-diode model that can be solved by a SPICE numerical simulation to comprehensively describe the monolithic perovskite/crystalline silicon(PVS/c-Si)tandem solar cells.We are able to reveal the effects of different efficiency-loss mechanisms based on the illuminated current density-voltage(J-V),semi-log dark J-V,and local ideality factor(m-V)curves.The effects of the individual efficiency-loss mechanism on the tandem cell’s efficiency are discussed,including the exp(V/VT)and exp(V/2VT)recombination,the whole cell’s and subcell’s shunts,and the Ohmic-contact or Schottky-contact of the intermediate junction.We can also fit a practical J-V curve and find a specific group of parameters by the trial-and-error method.Although the fitted parameters are not a unique solution,they are valuable clues for identifying the efficiency loss with the aid of the cell’s structure and experimental processes.This method can also serve as an open platform for analyzing other tandem solar cells by substituting the corresponding circuit models.In summary,we developed a simple and effective methodology to diagnose the efficiency-loss source of a monolithic PVS/c-Si tandem cell,which is helpful to researchers who wish to adopt the proper approaches to improve their solar cells.展开更多
Biodiversity is declining at an unprecedented rate.Many grassland species have been lost and are now of conservation concern.Identifying efficient biodiversity indicators is a key pillar of the global conservation str...Biodiversity is declining at an unprecedented rate.Many grassland species have been lost and are now of conservation concern.Identifying efficient biodiversity indicators is a key pillar of the global conservation strategy.Mongolian Lark(Melanocorypha mongolica) is a charismatic bird species abound in Mongolian steppes,and recent studies demonstrated that this species share similar habitat requirements an d likely co-occur with other grassland birds.We tested the hypothesis that Mongolian Lark would make a suitable indicator for different aspects of avian biodiversity.We made a large-scale bird survey in Inner Mongolia,and classified point counts into three groups based on different taxonomic,functional,and phylogenetic biodiversity measures.We identified species indicators of each group using the indicator value(IndVal) analysis,and examined the bird compositional difference among groups.Linear models were used for studying the patterns of occurrence of Mongolian Lark in relation to various biodiversity measures.Mon golian Lark showed the highest indicator value in comparison with other potential species indicators.Bird species richness is significantly higher at sites with Mongolian Lark,an d its occurrence was negatively correlated with species abundance,functional richness,Rao’s quadratic entropy and phylogenetic diversity.Mongolian Lark is therefore a suitable indicator species for avian biodiversity in Mongolian steppes.It is highly distinctive in flight and usually seen singly.The widespread distribution and charismatic appearance make it easy to monitor and adequate for citizen science,and may provide useful information on the paradigm of surrogacy in conservation ecology.展开更多
Objective Gastrointestinal stromal tumors(GISTs)can rapidly proliferate through angiogenesis.Previous studies indicated the potential influence of microRNA on the progression of tumor immature angiogenesis.This study ...Objective Gastrointestinal stromal tumors(GISTs)can rapidly proliferate through angiogenesis.Previous studies indicated the potential influence of microRNA on the progression of tumor immature angiogenesis.This study aimed to explore the specific mechanism by which microRNA-409-5p(miR-409-5p)contributes to GIST.Methods To identify genes potentially involved in the development and progression of GIST,the differences of miR-409-5p between tumors and adjacent tissues were first analyzed.Following this analysis,target genes were predicted.To further investigate the function of miRNA in GIST cells,two GIST cell lines(GIST-T1 and GIST882)were transfected with lentiviruses that stably expressed miR-409-5p and scrambled miRNA(negative control).Later,the cells were subjected to Western blotting and ELSA to determine any differences in angiogenesis-related genes.Results In GISTs,there was a decrease in the expression levels of miR-409-5p compared to the adjacent tissues.It was observed that the upregulation of miR-409-5p in GIST cell lines effectively inhibited the proteins hypoxia-inducible transcription factor 1β(HIF1β)and vascular endothelial growth factor A(VEGF-A).Further investigations revealed that miR-409-5p acted as an inhibitor of angiogenesis by binding to the 3′-UTR of Lysine-specific demethylase 4D(KDM4D)mRNA.Moreover,the combination of miR-409-5p with imatinib enhanced its inhibitory effect on angiogenesis.Conclusion This study demonstrated that the miRNA-409-5p/KDM4D/HIF1β/VEGF-A signaling pathway could serve as a novel target for the development of therapeutic strategies for the treatment of imatinib-resistance in GIST patients.展开更多
Read-write dependency is an important factor restricting software efficiency.Timing Speculative(TS)is a processing architecture aiming to improve energy efficiency of microprocessors.Timing error rate,influenced by th...Read-write dependency is an important factor restricting software efficiency.Timing Speculative(TS)is a processing architecture aiming to improve energy efficiency of microprocessors.Timing error rate,influenced by the read-write dependency,bottlenecks the voltage down-scaling and so the energy efficiency of TS processors.We proposed a method called Read-Write Dependency Aware Register Allocation.It is based on the Read-Write Dependency aware Interference Graph(RWDIG)conception.Registers are reallocated to loosen the read-write dependencies,so resulting in a reduction of timing errors.The traditional no operation(Nop)padding method is also redesigned to increase the distance value to above 2.We analyzed the dependencies of registers and maximized the average distance value of read and write dependencies.Experimental results showed that we can reduce all read-write dependency by Nop padding,as well as the overhead timing errors.An energy saving of approximately 7%was achieved.展开更多
Arteriovenous malformations(AVMs)are serious congenital vascular anomalies in which the arteries connect directly with veins without capillaries.This condition will continue to worsen without proper intervention and c...Arteriovenous malformations(AVMs)are serious congenital vascular anomalies in which the arteries connect directly with veins without capillaries.This condition will continue to worsen without proper intervention and cause ulcers,repeated hemorrhages,and even cardiac insufficiency.Primary treatment options for AVMs include surgery and interventional treatment;however,they are associated with high risk and recurrence rates.Recent studies revealed that excessive activation of the Ras/MAPK pathway can induce the formation and development of peripheral AVM,whereas MEK inhibitors can effectively control nidus progression,making them a potential novel treatment for AVM.This review provides an up-to-date overview of correlated laboratory and clinical research to provide information for further research and clinical practice.展开更多
基金supported by the Key Project of Tianjin Natural Science Foundation (No.21JCZDJC01080)the Tianjin Key Medical Discipline (Specialty) (TJYXZDX K-029A)+1 种基金the Academic Backbone of “Clinical Talent Training and Climbing Plan” of Tianjin Medical University and the Tianjin Health Research ProjectScience and Technology Development Fund of Nanjing Medical University (NMUB20210180)。
文摘BACKGROUND Chronic renal failure(CRF) patients are predisposed to arrhythmias, while the detailed mechanisms are unclear. We hypothesized the chronic inflammatory state of CRF patients may lead to cardiac sympathetic remodeling, increasing the incidence of ventricular arrhythmia(VA) and sudden cardiac death. And explored the role of atorvastatin and etanercept in this process.METHODS A total of 48 rats were randomly divided into sham operation group(Sham group), CRF group, CRF + atorvastatin group(CRF + statin group), and CRF + etanercept group(CRF + rhTNFR-Fcgroup). Sympathetic nerve remodeling was assessed by immunofluorescence of growth-associated protein 43(GAP-43) and tyrosine hydroxylase positive area fraction. Electrophysiological testing was performed to assess the incidence of VA by assessing the ventricular effective refractory period and ventricular fibrillation threshold. The levels of tumor necrosis factor-alpha(TNF-α) and interleukin-1beta were determined by Western blotting and enzyme-linked immunosorbent assay.RESULTS Echocardiogram showed that compared with the Sham group, left ventricular end-systolic diameter and ventricular weight/body weight ratio were significantly higher in the CRF group. Hematoxylin-eosin and Masson staining indicated that myocardial fibers were broken, disordered, and fibrotic in the CRF group. Western blotting, enzyme-linked immunosorbent assay,immunofluorescence and electrophysiological examination suggested that compared with the Sham group, GAP-43 and TNF-α proteins were significantly upregulated, GAP-43 and tyrosine hydroxylase positive nerve fiber area was increased, and ventricular fibrillation threshold was significantly decreased in the CRF group. The above effects were inhibited in the CRF + statin group and the CRF + rhTNFR-Fcgroup.CONCLUSIONS In CRF rats, TNF-α was upregulated, cardiac sympathetic remodeling was more severe, and the nephrogenic cardiac sympathetic remodeling existed. Atorvastatin and etanercept could downregulate the expression of TNF-α or inhibit its activity, thus inhibited the above effects, and reduced the occurrence of VA and sudden cardiac death.
基金supported by the National Natural Science Foundation of China,No.81473295(to ZMS),81373312(to XZ)and 81371028(to WCW)
文摘Chalcone is a plant metabolite widely found in fruits,vegetables,spices and tea,and has anti-tumor,anti-inflammation,immunomodulation,antibacterial and anti-oxidation activities,as well as many other pharmacological and biological effects.Our team has shown that its analogs have antioxidant activity,and oxidative stress is a pathological hallmark of retinal ischemia/reperfusion injury that can lead to retinal damage and visual loss.This investigation aims to identify a chalcone that protects retinal ganglion cells in vitro from the effects of oxidative stress and examine its mechanism.Rat retinal ganglion cell-5 cells were pretreated with chalcones and then exposed to tert-butyl hydroperoxide that causes oxidative damage.Controls received dimethyl sulfoxide only or tert-butyl hydroperoxide in dimethyl sulfoxide.Only（E）-3,4-dihydroxy-2′-methylether ketone（L2 H17）,of the five chalcone analogs,markedly increased the survival rate of oxidatively injured RGC-5 cells.Thus,subsequent experiments only analyzed the results of the L2 H17 intervention.Cell viability and apoptosis were measured.Intracellular superoxide dismutase and reactive oxygen species levels were used to assess induced oxidative stress.The mechanism of action by L2 H17 was explored by measuring the ER stress/UPR pathway and the expression and localization of Nrf2.All results demonstrated that L2 H17 could reduce the apoptosis of oxidatively injured cells,inhibit caspase-3 activity,increase Bcl-2 expression,decrease Bad expression,increase the activity of superoxide dismutase,inhibit the production of reactive oxygen species,increase Nrf2 immunoreactivity,and reduce the activating transcription factor 4,phospho-eukaryotic initiation factor 2 and CHOP expression.L2 H17 protects retinal ganglion cells induced by oxidative stress by regulating Nrf2,which indicates that it has the potential to become a drug for retinal ischemia/reperfusion.
基金National Natural Science Foundation of ChinaEducation Ministry of China.
文摘A kind of novel electroluminescence polymer has been prepared by oxidative-coupling polymerization of N-alkyl carbazole and trans-stilbene. The structure of the copolymers was characterized by elemental analysis, UV-vis and FTIR spectra. The copolymers have good solubility in chloroform and acetone. Fluorescence spectra showed that these polymers are blue-light emitting materials.
文摘Objective:To detect serum contents of IgE, Eotaxin and explore the relationship between serum IgE, Eotaxin and asthma severity.Methods: 140 cases of asthma children diagnosed in our hospital during January 2017 to March 2018 were chosen as Asthma group, 100 cases of healthy children vaccinated in our hospital at the same time were chosen as Normal control group. Serum contents of IgE, Eotaxin, inflammatory mediators and airway remodeling indexes were detected in two groups. Correlation analysis was used to evaluate the relationship between serum IgE, Eotaxin content and severity of asthma.Results: Serum contents of IgE and Eotaxin in Asthma group were significantly higher than those in Normal control group, and contents of IL-9, IL-17, IL-18 and IL-33 were higher than those in Normal control group, IL-35 was lower than that in Normal control group;serum contents of MMP-2, MMP-9, TGF-β and VEGF in Asthma group were significantly higher than those in Normal control group (P<0.05). Pearson test found that serum contents of IgE, Eotaxin in asthma children were positively correlated with inflammatory mediators such as IL-9, IL-17, IL-18, IL-33, negatively correlated with IL-35;positively correlated with airway remodeling indexes such as MMP-2, MMP-9, TGF-β and VEGF.Conclusion: Serum contents of IgE, Eotaxin in asthma children are significantly higher than those in normal children, and the specific contents were closely related to the degree of systemic inflammatory response and airway remodeling.
文摘Objective:To investigate the efficacy of botulinum toxin type A combined with nanofat in the treatment of facial rejuvenation.Methods:from January 2020 to January 2021,20 patients with facial wrinkles in our hospital(The First Affiliated Hospital of Dalian Medical University)were treated with botulinum toxin type A combined with nanofat,and the effect,duration and complications were observed.Results:After 1,3 and 6 months of injection,the effective rates were 90%,75%and 40%,respectively;there was no occurrence of postoperative infection,fat mass,granuloma,and other adverse reactions.Conclusion:Botulinum toxin type A combined with nanofat filling has a clear therapeutic effect in the facial rejuvenation treatment,and can improve the skin texture while improving wrinkles,which is worthy of further popularization and application in clinic.
基金supported by grants from the National Natural Science Foundation of China(Grant No.82060483)Guangxi Research Foundation for Science&Technology Base and Talent Special(Grant No.AD19110079)Natural Science Foundation of Guangxi Province(Grant No.2020GXNSFBA238002).
文摘Background:Cytochrome b561(CYB561)plays a critical role in neuroendocrine function,cardiovascular regulation,and tumor growth;however,the prognostic value of CYB561 in patients with breast cancer and the relationship between CYB561 expression and immune infiltration in breast cancer remain unclear.Methods:The mRNA expression and clinical data of patients with breast cancer were obtained from The Cancer Genome Atlas database.Functional enrichment analysis was used to explore underlying biological functions associated with CYB561.The methylation status of CYB561 was analyzed using the MethSurv database.The enrichment score of immune cell infiltration for CYB561 in breast cancer was calculated using single-sample gene set enrichment analysis.The prognostic value of CYB561 was evaluated using Kaplan-Meier method and Cox regression analysis.Based on the results of the multivariate Cox analysis,a nomogram was constructed to predict the effect of CYB561 expression on overall survival(OS).Results:The results showed that CYB561 was highly expressed in breast cancer tissues.Hypomethylation of CYB561 is associated with an unfavorable prognosis.In multivariate Cox regression analysis,CYB561 was an independent prognostic factor for OS.Functional enrichment analysis indicated that estrogen signaling pathway,inflammatory response,KRAS signaling pathway,epithelial-mesenchymal transition,leukocyte migration,and regulation of lymphocyte activation were strongly enriched in the low CYB561 expression group.Additionally,CYB561 expression was negatively correlated with immune infiltration of B cells,plasmacytoid dendritic cells,dendritic cells,and neutrophils.Conclusion:CYB561 may serve as a potential biomarker for breast cancer diagnosis and prognosis.
基金This work was supported by Zhejiang Energy Group(znkj-2018-118)Key Research and Development Program of Zhejiang Province(2021C01006)+5 种基金Key Project of Zhejiang Province(2021C04009)Science and technology projects in Liaoning Province 2021(2021JH1/10400104)Ningbo“Innovation 2025”Major Project(2020Z098)National Key R&D Program of China(2018YFB1500403)National Natural Science Foundation of China(61974178,61874177,62004199)Youth Innovation Promotion Association(2018333).
文摘In this work,we developed a simple and direct circuit model with a dual two-diode model that can be solved by a SPICE numerical simulation to comprehensively describe the monolithic perovskite/crystalline silicon(PVS/c-Si)tandem solar cells.We are able to reveal the effects of different efficiency-loss mechanisms based on the illuminated current density-voltage(J-V),semi-log dark J-V,and local ideality factor(m-V)curves.The effects of the individual efficiency-loss mechanism on the tandem cell’s efficiency are discussed,including the exp(V/VT)and exp(V/2VT)recombination,the whole cell’s and subcell’s shunts,and the Ohmic-contact or Schottky-contact of the intermediate junction.We can also fit a practical J-V curve and find a specific group of parameters by the trial-and-error method.Although the fitted parameters are not a unique solution,they are valuable clues for identifying the efficiency loss with the aid of the cell’s structure and experimental processes.This method can also serve as an open platform for analyzing other tandem solar cells by substituting the corresponding circuit models.In summary,we developed a simple and effective methodology to diagnose the efficiency-loss source of a monolithic PVS/c-Si tandem cell,which is helpful to researchers who wish to adopt the proper approaches to improve their solar cells.
基金the National Natural Science Foundation of China(No.32201304)the Fundamental Research Funds for the Central Universities(No.2412022QD026).
文摘Biodiversity is declining at an unprecedented rate.Many grassland species have been lost and are now of conservation concern.Identifying efficient biodiversity indicators is a key pillar of the global conservation strategy.Mongolian Lark(Melanocorypha mongolica) is a charismatic bird species abound in Mongolian steppes,and recent studies demonstrated that this species share similar habitat requirements an d likely co-occur with other grassland birds.We tested the hypothesis that Mongolian Lark would make a suitable indicator for different aspects of avian biodiversity.We made a large-scale bird survey in Inner Mongolia,and classified point counts into three groups based on different taxonomic,functional,and phylogenetic biodiversity measures.We identified species indicators of each group using the indicator value(IndVal) analysis,and examined the bird compositional difference among groups.Linear models were used for studying the patterns of occurrence of Mongolian Lark in relation to various biodiversity measures.Mon golian Lark showed the highest indicator value in comparison with other potential species indicators.Bird species richness is significantly higher at sites with Mongolian Lark,an d its occurrence was negatively correlated with species abundance,functional richness,Rao’s quadratic entropy and phylogenetic diversity.Mongolian Lark is therefore a suitable indicator species for avian biodiversity in Mongolian steppes.It is highly distinctive in flight and usually seen singly.The widespread distribution and charismatic appearance make it easy to monitor and adequate for citizen science,and may provide useful information on the paradigm of surrogacy in conservation ecology.
基金supported by the National Natural Science Foundation of China(No.81372323 and No.81802426).
文摘Objective Gastrointestinal stromal tumors(GISTs)can rapidly proliferate through angiogenesis.Previous studies indicated the potential influence of microRNA on the progression of tumor immature angiogenesis.This study aimed to explore the specific mechanism by which microRNA-409-5p(miR-409-5p)contributes to GIST.Methods To identify genes potentially involved in the development and progression of GIST,the differences of miR-409-5p between tumors and adjacent tissues were first analyzed.Following this analysis,target genes were predicted.To further investigate the function of miRNA in GIST cells,two GIST cell lines(GIST-T1 and GIST882)were transfected with lentiviruses that stably expressed miR-409-5p and scrambled miRNA(negative control).Later,the cells were subjected to Western blotting and ELSA to determine any differences in angiogenesis-related genes.Results In GISTs,there was a decrease in the expression levels of miR-409-5p compared to the adjacent tissues.It was observed that the upregulation of miR-409-5p in GIST cell lines effectively inhibited the proteins hypoxia-inducible transcription factor 1β(HIF1β)and vascular endothelial growth factor A(VEGF-A).Further investigations revealed that miR-409-5p acted as an inhibitor of angiogenesis by binding to the 3′-UTR of Lysine-specific demethylase 4D(KDM4D)mRNA.Moreover,the combination of miR-409-5p with imatinib enhanced its inhibitory effect on angiogenesis.Conclusion This study demonstrated that the miRNA-409-5p/KDM4D/HIF1β/VEGF-A signaling pathway could serve as a novel target for the development of therapeutic strategies for the treatment of imatinib-resistance in GIST patients.
基金This work was supported by the Project of Hunan Social Science Achievement Evaluation Committee(XSP20YBZ090,Sheng Xiao,2020).
文摘Read-write dependency is an important factor restricting software efficiency.Timing Speculative(TS)is a processing architecture aiming to improve energy efficiency of microprocessors.Timing error rate,influenced by the read-write dependency,bottlenecks the voltage down-scaling and so the energy efficiency of TS processors.We proposed a method called Read-Write Dependency Aware Register Allocation.It is based on the Read-Write Dependency aware Interference Graph(RWDIG)conception.Registers are reallocated to loosen the read-write dependencies,so resulting in a reduction of timing errors.The traditional no operation(Nop)padding method is also redesigned to increase the distance value to above 2.We analyzed the dependencies of registers and maximized the average distance value of read and write dependencies.Experimental results showed that we can reduce all read-write dependency by Nop padding,as well as the overhead timing errors.An energy saving of approximately 7%was achieved.
基金sponsored by 2023 Shanghai Jiao Tong University Medical-Industrial Intersection Key Project(grant no.YG2023ZD13):Screening of Novel Targeted Inhibitors Based on Genetic Vascular Malformation Organoid Models.
文摘Arteriovenous malformations(AVMs)are serious congenital vascular anomalies in which the arteries connect directly with veins without capillaries.This condition will continue to worsen without proper intervention and cause ulcers,repeated hemorrhages,and even cardiac insufficiency.Primary treatment options for AVMs include surgery and interventional treatment;however,they are associated with high risk and recurrence rates.Recent studies revealed that excessive activation of the Ras/MAPK pathway can induce the formation and development of peripheral AVM,whereas MEK inhibitors can effectively control nidus progression,making them a potential novel treatment for AVM.This review provides an up-to-date overview of correlated laboratory and clinical research to provide information for further research and clinical practice.
