BACKGROUND Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep.Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia,and long-term use i...BACKGROUND Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep.Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia,and long-term use is prone to drug resistance and other adverse reactions.Acupuncture has a good curative effect and unique advantages in the treatment of insomnia.AIM To explore the molecular mechanism of acupuncture at Back-Shu point for the treatment of insomnia.METHODS We first prepared a rat model of insomnia,and then carried out acupuncture for 7 consecutive days.After treatment,the sleep time and general behavior of the rats were determined.The Morris water maze test was used to assess the learning ability and spatial memory ability of the rats.The expression levels of inflammatory cytokines in serum and the hippocampus were detected by ELISA.qRTPCR was used to detect the mRNA expression changes in the ERK/NF-κB signaling pathway.Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1,MEK-2,ERK1/2 and NF-κB.RESULTS Acupuncture can prolong sleep duration,and improve mental state,activity,diet volume,learning ability and spatial memory.In addition,acupuncture increased the release of 1L-1β,1L-6 and TNF-αin serum and the hippocampus and inhibited the mRNA and protein expression of the ERK/NF-κB signaling pathway.CONCLUSION These findings suggest that acupuncture at Back-Shu point can inhibit the ERK/NF-κB signaling pathway and treat insomnia by increasing the release of inflammatory cytokines in the hippocampus.展开更多
Two cases of herpes zoster neuralgia treated by shallow needling combined with electroacupuncture were introduced. The literature basis and possible mechanisms were discussed.
From our clinical practice,it was found that,using the back-shu acupoints as the main ones together with the symptomized acupoints according to the different syndromes,the therapeutic effect in the treatment of arrhyt...From our clinical practice,it was found that,using the back-shu acupoints as the main ones together with the symptomized acupoints according to the different syndromes,the therapeutic effect in the treatment of arrhythmia was good.Our experience in acupuncture treatment for arrhythmia is introduced as follows with two cases attached.展开更多
One case of seborrheic dermatitis,male patient,aged 73 years is reported.After treated with acupuncture,penetrating moxibustion and bloodletting therapies,skin lesions on the neck and shoulder regions disappeared,the ...One case of seborrheic dermatitis,male patient,aged 73 years is reported.After treated with acupuncture,penetrating moxibustion and bloodletting therapies,skin lesions on the neck and shoulder regions disappeared,the epidermis returned to normal and itching disappeared.There was no recurrence in the follow-up visit 3 months later.Hence,acupuncture,combined with penetrating moxibustion and bloodletting has a definite effect on this case in the patient and it is worthy of promotion and application in clinical practice.展开更多
文摘BACKGROUND Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep.Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia,and long-term use is prone to drug resistance and other adverse reactions.Acupuncture has a good curative effect and unique advantages in the treatment of insomnia.AIM To explore the molecular mechanism of acupuncture at Back-Shu point for the treatment of insomnia.METHODS We first prepared a rat model of insomnia,and then carried out acupuncture for 7 consecutive days.After treatment,the sleep time and general behavior of the rats were determined.The Morris water maze test was used to assess the learning ability and spatial memory ability of the rats.The expression levels of inflammatory cytokines in serum and the hippocampus were detected by ELISA.qRTPCR was used to detect the mRNA expression changes in the ERK/NF-κB signaling pathway.Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1,MEK-2,ERK1/2 and NF-κB.RESULTS Acupuncture can prolong sleep duration,and improve mental state,activity,diet volume,learning ability and spatial memory.In addition,acupuncture increased the release of 1L-1β,1L-6 and TNF-αin serum and the hippocampus and inhibited the mRNA and protein expression of the ERK/NF-κB signaling pathway.CONCLUSION These findings suggest that acupuncture at Back-Shu point can inhibit the ERK/NF-κB signaling pathway and treat insomnia by increasing the release of inflammatory cytokines in the hippocampus.
文摘Two cases of herpes zoster neuralgia treated by shallow needling combined with electroacupuncture were introduced. The literature basis and possible mechanisms were discussed.
文摘From our clinical practice,it was found that,using the back-shu acupoints as the main ones together with the symptomized acupoints according to the different syndromes,the therapeutic effect in the treatment of arrhythmia was good.Our experience in acupuncture treatment for arrhythmia is introduced as follows with two cases attached.
文摘One case of seborrheic dermatitis,male patient,aged 73 years is reported.After treated with acupuncture,penetrating moxibustion and bloodletting therapies,skin lesions on the neck and shoulder regions disappeared,the epidermis returned to normal and itching disappeared.There was no recurrence in the follow-up visit 3 months later.Hence,acupuncture,combined with penetrating moxibustion and bloodletting has a definite effect on this case in the patient and it is worthy of promotion and application in clinical practice.
