Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pai...Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.展开更多
目的利用静息态功能磁共振成像技术(resting-state functional magnetic resonance imaging,rs-fMRI)局部一致性(regional homogeneity,ReHo)的方法探讨首发抑郁症患者静息态脑活动的变化,并分析汉密尔顿抑郁量表(the scores of 17-item...目的利用静息态功能磁共振成像技术(resting-state functional magnetic resonance imaging,rs-fMRI)局部一致性(regional homogeneity,ReHo)的方法探讨首发抑郁症患者静息态脑活动的变化,并分析汉密尔顿抑郁量表(the scores of 17-item Hamilton Rating Scale for Depression,HAMD-17)评分与ReHo值异常脑区的相关性。材料与方法选取首发抑郁症患者14例和健康对照组16例,进行静息态功能磁共振扫描,运用ReHo方法分析数据,两独立样本t检验比较两组受试者的ReHo值,并对首发抑郁症组HAMD-17评分与差异脑区ReHo均值进行相关分析。结果与对照组相比,首发抑郁症组患者右前扣带回、右中央后回的ReHo值减低(Alphasim校正,校正后P<0.05);首发抑郁症患者右扣带前回ReHo均值与HAMD-17评分呈负相关(r=-0.566,P=0.035)。结论静息状态下首发抑郁症患者右侧前扣带回及右侧中央后回同步一致性活动减低,且可能与抑郁症的发病机制有关。展开更多
基金supported by the National Natural Science Foundation of China,No.81671891
文摘Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.
文摘目的利用静息态功能磁共振成像技术(resting-state functional magnetic resonance imaging,rs-fMRI)局部一致性(regional homogeneity,ReHo)的方法探讨首发抑郁症患者静息态脑活动的变化,并分析汉密尔顿抑郁量表(the scores of 17-item Hamilton Rating Scale for Depression,HAMD-17)评分与ReHo值异常脑区的相关性。材料与方法选取首发抑郁症患者14例和健康对照组16例,进行静息态功能磁共振扫描,运用ReHo方法分析数据,两独立样本t检验比较两组受试者的ReHo值,并对首发抑郁症组HAMD-17评分与差异脑区ReHo均值进行相关分析。结果与对照组相比,首发抑郁症组患者右前扣带回、右中央后回的ReHo值减低(Alphasim校正,校正后P<0.05);首发抑郁症患者右扣带前回ReHo均值与HAMD-17评分呈负相关(r=-0.566,P=0.035)。结论静息状态下首发抑郁症患者右侧前扣带回及右侧中央后回同步一致性活动减低,且可能与抑郁症的发病机制有关。
