A novel histone methyltransferase gene Cg SDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening

The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human health.It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo.However,the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood.In this study,we identified a novel histone methyltransferase gene,CgSDG40,involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo,red-fleshed pummelo and extreme-colored F1 hybrids from a segregated pummelo population.Expression of CgSDG40 corresponded to flesh color change and was highly coexpressed with CgPSY1.Interestingly,CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions,sharing a partially overlapping promoter region.Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus.Overexpression of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild type.In addition,expression of CgPSY1 was significantly activated in overexpression lines relative to wild type.Taken together,our findings reveal a novel histone methyltransferase regulator,CgSDG40,involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.

N^(6)-甲基腺苷修饰在肿瘤程序性细胞死亡中的作用

非突变表观遗传重编程是肿瘤的关键特征之一,抵抗程序性细胞死亡是肿瘤的另一关键特征。作为体内最丰富的转录后表观遗传修饰方式,N^(6)-甲基腺苷(N^(6)-methyladenosine,m^(6)A)通过靶向调节程序性细胞死亡关键因子在肿瘤细胞凋亡、自噬、焦亡、铁死亡、坏死性凋亡、铜死亡中发挥重要作用。现已有靶向异常DNA甲基化、组蛋白修饰的表观遗传调节剂应用于临床,靶向m^(6)A修饰调控药物仍待探索。本文阐述了m^(6)A修饰调控肿瘤细胞程序性死亡的机制,旨在为通过调节m^(6)A修饰水平介导肿瘤细胞死亡这一潜在肿瘤治疗策略提供理论基础。

Comprehensive Survey of the Landscape of Digital Twin Technologies and Their Diverse Applications

The concept of the digital twin,also known colloquially as the DT,is a fundamental principle within Industry 4.0 framework.In recent years,the concept of digital siblings has generated considerable academic and practical interest.However,academia and industry have used a variety of interpretations,and the scientific literature lacks a unified and consistent definition of this term.The purpose of this study is to systematically examine the definitional landscape of the digital twin concept as outlined in scholarly literature,beginning with its origins in the aerospace domain and extending to its contemporary interpretations in the manufacturing industry.Notably,this investigationwill focus on the research conducted on Industry 4.0 and smartmanufacturing,elucidating the diverse applications of digital twins in fields including aerospace,intelligentmanufacturing,intelligent transportation,and intelligent cities,among others.

Mitigation of polycyclic aromatic hydrocarbons(PAHs)in roasted beef patties by cold plasma treatment and products quality evaluation

The cold plasma(CP)technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon(PAH)in this investigation.Two different CP treatments methods were implemented in the production of beef patties,to investigate their inhibition and degradation capacity on PAHs.With 5 different cooking oils and fats addition,the inhibition mechanism of in-package cold plasma(ICP)pretreatment was explored from the aspect of raw patties fatty acids composition variation.The results of principal component analysis showed that the first two principal components accounted for more than 80%of the total variation in the original data,indicating that the content of saturated fatty acids was significantly positively correlated with the formation of PAHs.ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids,which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased.Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls,utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality.In conclusion,CP treatment degraded PAHs through stepwise ring-opening oxidation in 2 reported pathways,the toxicity of PAHs contaminated products was alleviated after CP treatment.

Two-dimensional Sb net generated nontrivial topological states in SmAgSb_(2) probed by quantum oscillations

The REAgSb_(2)(RE = rare earth and Y) family has drawn considerable research interest because the two-dimensional Sb net in their crystal structures hosts topological fermions and hence rich topological properties. We report herein the magnetization and magnetotransport measurements of SmAgSb_(2) single crystal, which unveil very large magnetoresistance and high carrier mobility up to 6.2 × 10^(3)% and 5.58 × 10^(3)cm^(2)·V^(-1)·s^(-1), respectively. The analysis of both Shubnikov–de Haas and de Haas–van Alphen quantum oscillations indicates nontrivial Berry phases in the paramagnetic state while trivial Berry curvature in the antiferromagnetic state, indicating a topological phase transition induced by the antiferromagnetic order. It is also supported by the first-principles calculations. The results not only provide a new interesting topological material but also offer valuable insights into the correlation between magnetism and nontrivial topological states.

The Epstein-Barr virus-miRNA-BART6-5p regulates TGF-β/SMAD4 pathway to induce glycolysis and enhance proliferation and metastasis of gastric cancer cells

Background:EBV-miR-BARTs exhibit significant relevance in epithelial tumors,particularly in EBVassociated gastric and nasopharyngeal cancers.However,their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored.Material and Methods:Initially,EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines.Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45,and downregulation in EBVpositive cells(SUN-719).The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation,migration,and glycolytic processes,with the TGF-β/SMAD4 signaling pathway value clarified using a TGF-βinhibitor.Results:EBV-miRNA-BART6-5p exhibits pronounced upregulation in EBV-associated gastric cancer tissues and EBV-positive cells,while its target gene SMAD4 demonstrates downregulated expression.Upregulation of it can promote the proliferation and migration of gastric cancer cells.Additionally,We found EBV-miRNA-BART6-5p promotes glycolysis of gastric cancer cells.Inhibition of the TGF-β/SMAD4 signaling pathway resulted in suppressed proliferation and migration of gastric cancer cells,concomitant with a diminished glycolytic capacity.Conclusion:In this study,we found that EBV-miRNA-BART6-5p can target SMAD4,effectively increasing glycolysis in gastric cancer cells by regulating the TGF-β/SMAD4 signaling pathway,thereby enhancing the proliferation and metastasis of gastric cancer cells.Our findings may offer new insights into the metabolic aspects of gastric cancer.

Medical imaging for the diagnosis,recurrence and metastasis evaluation of clear cell sarcoma

Clear cell sarcoma(CCS)of soft tissue is extremely rare,accounting for approximately 1%of all soft tissue tumours.It is very difficult to diagnose CCS based on clinical manifestations.Magnetic resonance imaging(MRI)provides highresolution images of soft tissues and pathological features such as mucus,necrosis,bleeding,and fat through high and low signals on T1 weighted image(T1WI)and T2 weighted image(T2WI).On the other hand,the paramagnetism of melanin in CCS shortens the relaxation time of T1 and T2,and high signal intensity on T1WI and low signal intensity on T2WI can be found.This is different from most other soft tissue sarcomas.At present,the treatment method for CCS is surgical resection.MRI can effectively display the tumour edge,extent of surrounding oedema,and extent of fat involvement,which is highly important for guiding surgical resection and predicting postoperative recurrence.As an invasive sarcoma,CCS has a high risk of metastasis.Regardless of the pathological condition of the resected tumour,MRI or computed tomography(CT)should be performed every 1-2 years to assess recurrence at the primary site and to screen for metastasis in the lungs,liver,and bones.If necessary,PET-CT can be performed to evaluate the overall condition of the patient.

Small-diameter acellular porcine corneal stroma for peripheral corneal ulceration treatment

AIM:To evaluate the clinical efficacy of small-diameter acellular porcine corneal stroma(SAPS)for the treatment of peripheral corneal ulceration(PCU).METHODS:This retrospective clinical study included 18 patients(18 eyes)with PCU between April 2018 and December 2020.All patients had PCU and underwent lamellar keratoplasty with SAPS.Observation indicators included preoperative and postoperative best-corrected visual acuity(BCVA)and transparency of SAPS.The infection control rate in the surgical eye-lesion area was also calculated.RESULTS:Eighteen patients underwent lamellar keratoplasty with SAPS to treat PCU.None of the patients experienced rejection after 6mo(18/18)and 12mo(16/16)of follow-up.The BCVA(0.47±0.30)at the 6mo followup after operation was significantly improved compared with the baseline(0.99±0.80),and the difference was statistically significant(Z=-3.415,P<0.05).The BCVA at the 12mo follow-up after operation was not statistically significant compared to the 6mo(Z=0,P=1).With time,the SAPS graft gradually became transparent.At the 6mo(18/18)and 12mo(16/16)follow-up,none of the patients had recurrent corneal infection.CONCLUSION:SAPS is clinically effective in the treatment of PCU,improving the patient’s BCVA and reducing the incidence of rejection after keratoplasty.

基于卷积神经网络的绝缘子异常检测方法

输电网络是电网“大动脉”,而绝缘子运行状态直接影响整条线路的运行安全和可靠性。为了快速评估绝缘子运行状态,有效提升运行可靠性。传统的故障识别主要依赖人工经验,识别的准确性依赖工程师经验。为此,该文提出一种基于卷积神经网络的电网故障识别方法,相较直接使用故障数据的传统方法,先将故障波形转换为图片,充分发挥卷积神经网络强大的图片处理能力。实验结果表明,该文所训练的卷积神经网络具有很好的准确率,正确率可达到98%。

Safety and efficacy of peroral endoscopic myotomy for treating achalasia in pediatric and geriatric patients:A

BACKGROUND As a less invasive technique,peroral endoscopic myotomy(POEM)has recently been widely accepted for treating achalasia with an excellent safety profile,durability,and efficacy in adults.In pediatric and geriatric patients,the treatment is more difficult.AIM To discuss the clinical outcomes of POEM in pediatric and geriatric patients with achalasia.METHODS We conducted a comprehensive search of PubMed,Embase and Cochrane Library databases from inception to July 2024.The primary outcomes were technical and clinical success.Secondary outcomes of interest included adverse events and gastroesophageal reflux disease(GERD).The pooled event rates were calculated by comprehensive meta-analysis software.RESULTS A total of 32 studies with 547 pediatric patients and 810 geriatric patients were included in this study.The pooled event rates of technical success,clinical success,GERD and adverse events of POEM for treating achalasia in pediatric patients were 97.1%[95%confidence interval(CI):95.0%-98.3%;I²=0%;P<0.000],93.2%(95%CI:90.5%-95.2%;I²=0%;P<0.000),22.3%(95%CI:18.4%-26.7%;I²=43.874%;P<0.000)and 20.4%(95%CI:16.6%-24.8%;I²=67.217%;P<0.000),respectively.Furthermore,in geriatric patients,the pooled event rates were 97.7%(95%CI:95.8%-98.7%;I²=15.200%;P<0.000),93.2%(95%CI:90.3%-95.2%;I²=0%;P<0.000),23.9%(95%CI:19.4%-29.1%;I²=75.697%;P<0.000)and 10.8%(95%CI:8.3%-14.0%;I²=62.938%;P<0.000],respectively.CONCLUSION Our findings demonstrated that POEM was an effective and safe technique for pediatric and geriatric patients with achalasia.

一种基于选权迭代的样本数据自动清洗方法

基于数字线划图和真正射影像可以自动生成大量满足深度学习要求的样本数据,但是其中存在部分错误信息,会导致神经网络模型训练难度加大,并且限制地物提取精度提升。因此,提出一种基于选权迭代的样本数据自动清洗方法,首先构建数据清洗深度神经网络模型,并给出基于选权迭代的网络训练方法,该方法打破传统网络模型训练时认为所有样本对损失函数计算权重相同的假设,利用数据清洗网络模型训练过程中样本预测精度作为样本权重并带入网络训练中,再通过迭代训练不断更新样本权重,最终将权重低的样本剔除,以实现自动数据清洗和样本库精化。使用数据清洗前后的样本库,对5种经典语义分割网络模型进行训练和精度对比实验,结果表明,利用数据清洗后的样本库训练模型,建筑提取精度平均提高2.36%,道路提取精度平均提高3.48%,水体提取精度平均提高1.88%,证明提出的数据清洗方法可以有效提高网络模型的提取精度。

Assessment of visual outcomes of cataract surgery in Tujia nationality in Xianfeng County, China

AIM: To evaluate the visual outcome and factors influencing visual outcome of manual small incision cataract surgery(MSICS) in the rural area in the Xianfeng County.METHODS: Eighty-two eyes of 82 patients who underwent cataract surgery performed by using MSICS technique were identified. Data collected included each patient’s age, gender, the level of education. Uncorrected and corrected distance visual acuity(UDVA and CDVA) at presentation and at 1, 6, 8wk postoperatively, pre-existing eye disease, operative findings and complications, the risk factors were evaluated.RESULTS: In 82 patients, the average age was 69.6±0.6y, illiterate were 52(63.4%). Of 82 eyes, pseudophakia was present in 77 eyes(93.9%). At 1wk postoperatively,47 eyes(57.3%) had the UDVA of ≥6/18, and 52 eyes(63.4%) had the CDVA of ≥6/18. At 6 to 8wk postoperatively, 50 eyes(61.0%) had UDVA of ≥6/18, and57 eyes(69.5%) had the CDVA of ≥6/18. Postoperative visual status was significantly related to the co-morbidities, such as corneal pathology, glaucoma(P 【0.001).Operative complications, such as posterior capsule opacity and cystoid macular edema were main operative cause for the poor visual outcome.CONCLUSION: MSICS provides a good visual recovery in our study but the vision outcome did not fulfill the standards proposed by WHO, which highlights the need for an improvement in local socioeconomic understanding, population education and surgery quality.

25G玻璃体切除联合DEX治疗PDR继发玻璃体积血伴DME

目的:评价25G玻璃体切除(PPV)联合地塞米松玻璃体内植入剂(DEX)治疗增殖性糖尿病视网膜病变(PDR)继发玻璃体积血伴糖尿病性黄斑水肿(DME)的临床效果。方法:前瞻性临床病例研究。选取2020-07/2022-01天津市眼科医院收治的PDR继发玻璃体积血伴DME的患者40例40眼,所有患者均行玻璃体切除和白内障超声乳化手术,随机分为PPV组(20眼)和PPV+DEX组(20眼)。比较两组患者术前,术后1、3、6mo最佳矫正视力(BCVA)、眼压及黄斑中心凹厚度(CMT)。结果:所有患者均完成术后6mo随访。术后1、3、6mo PPV+DEX组患者BCVA均优于PPV组(P<0.05)。术后1mo,PPV+DEX组CMT低于PPV组(P<0.05)。随访6mo,PPV组术后出现视网膜新生血管或CMT消退小于5%者8眼行补充抗VEGF治疗,PPV+DEX组仅有1眼(P<0.05)。结论:玻璃体切除手术联合应用地塞米松植入剂可以产生协同效应,为PDR继发玻璃体积血伴DME的患者提供更优的治疗效果。

自支撑过渡金属海水电解析氧催化剂研究进展

海水电解是一种很有前景的可持续绿氢生产技术。然而,由于受到缓慢的动力学、阳极上竞争性的氯释放反应、氯离子腐蚀和电极表面中毒等的影响,致使阳极材料的性能和耐久性下降,析氧反应(OER)的选择性降低。与传统的粉末催化剂相比,自支撑纳米阵列材料具有较低的界面电阻、较大的活性表面和优异的稳定性,已成为先进的催化剂。特别是在需要高电流密度的实际大规模制氢应用中,自支撑催化剂比粉末催化剂更具优势。电解过程中,在电极表面所产生气泡的强烈冲击下,粉末状纳米材料很容易剥离,导致催化活性降低,甚至频繁更换催化剂。相比之下,自支撑纳米材料的活性物质和基底之间具有很强的粘附性,确保了良好的电子导电性和高机械稳定性,有利于长期和循环使用。本文综述了用于海水电解自支撑过渡金属催化剂的最新进展,包括(氧)氢氧化物、氮化物、磷化物、硫族化物等。为确保OER过程中的高活性和高选择性,着重总结了各析氧催化剂在应对耐腐蚀性和屏蔽竞争反应方面所采取的策略。通常,构建具有高孔隙率和粗糙度的3D多孔纳米结构可以使催化剂具有较大的表面积和丰富的活性位点,是提高传质、OER活性和催化效率的有效策略。其次,催化剂表面的Cl−阻挡层,特别是兼具催化活性和保护作用的保护层,可以有效抑制Cl−的竞争性氧化和腐蚀,提高催化剂的催化活性、选择性和稳定性。此外,设计具有超亲水和超疏水表面的催化材料,以增加电解质的渗透性,促进活性位点的有效利用,并避免大量气泡的积累。最后,简要总结了OER催化剂在海水电解中的发展前景及建议。具体来说,海水电解的介质应从模拟盐水转移到天然海水中。鉴于天然海水电解中面临的诸多挑战,除了设计和合成具有高活性、高选择性和高稳定性的自支撑催化剂外,开发简单、低成本的天然海水预处理技术以最大限度地减少腐蚀和中毒问题也是海水电解未来发展的重要课题。更重要的是,应合理构建自支撑OER电催化剂的标准化评价体系。评估过程中应充分考虑催化剂内在活性、可达活性位点密度、尺寸、质量负荷、基质效应和试验条件等因素。

Factors associated with heterochronic gastric cancer development post-endoscopic mucosal dissection in early gastric cancer patients

BACKGROUND Endoscopic mucosal resection is an innovative method for treating early gastric cancer and has been widely used in clinical practice.AIM To analyze the factors associated with the development of heterochronic gastric cancer in patients with early gastric cancer who had undergone endoscopic mucosal dissection(EMD).METHODS A cohort of patients with early gastric cancer treated using EMD was retrospectively analyzed,and patients who developed heterochronic gastric cancer after the surgery were compared with those who did not.The effects of patient age,sex,tumor size,pathological type,and surgical technique on the development of heterochronic gastric cancer were assessed using statistical analysis.RESULTS Of the 300 patients with early gastric cancer,150 patients developed heterochronic gastric cancer after EMD.Statistical analysis revealed that patient age(P value=XX),sex(P value=XX),tumor size(P value=XX),pathological type(P value=XX),and surgical technique(P value=XX)were significantly associated with the occurrence of heterochronic gastric cancer.CONCLUSION Age,sex,tumor size,pathological type,and surgical technique are key factors influencing the occurrence of heterochronic gastric cancer after EMD in patients with early gastric cancer.To address these factors,postoperative follow-up and management should be strengthened to improve the prognosis and survival rate of patients.

Simulation study of hydrogen sulfide removal in underground gas storage converted from the multilayered sour gas field

A simulation study was carried out to investigate the temporal evolution of H_(2)S in the Huangcaoxia underground gas storage (UGS), which is converted from a depleted sulfur-containing gas field. Based on the rock and fluid properties of the Huangcaoxia gas field, a multilayered model was built. The upper layer Jia-2 contains a high concentration of H_(2)S (27.2 g/m^(3)), and the lower layer Jia-1 contains a low concentration of H_(2)S (14.0 mg/m^(3)). There is also a low-permeability interlayer between Jia-1 and Jia-2. The multi-component fluid characterizations for Jia-1 and Jia-2 were implemented separately using the Peng-Robinson equation of state in order to perform the compositional simulation. The H_(2)S concentration gradually increased in a single cycle and peaked at the end of the production season. The peak H_(2)S concentration in each cycle showed a decreasing trend when the recovery factor (RF) of the gas field was lower than 70%. When the RF was above 70%, the peak H_(2)S concentration increased first and then decreased. A higher reservoir RF, a higher maximum working pressure, and a higher working gas ratio will lead to a higher H_(2)S removal efficiency. Similar to developing multi-layered petroleum fields, the operation of multilayered gas storage can also be divided into multi-layer commingled operation and independent operation for different layers. When the two layers are combined to build the storage, the sweet gas produced from Jia-1 can spontaneously mix with the sour gas produced from Jia-2 within the wellbore, which can significantly reduce the overall H_(2)S concentration in the wellstream. When the working gas volume is set constant, the allocation ratio between the two layers has little effect on the H_(2)S removal. After nine cycles, the produced gas’s H_(2)S concentration can be lowered to 20 mg/m^(3). Our study recommends combining the Jia-2 and Jia-1 layers to build the Huangcaoxia underground gas storage. This plan can quickly reduce the H_(2)S concentration of the produced gas to 20 mg/m^(3), thus meeting the gas export standards as well as the HSE (Health, Safety, and Environment) requirements in the field. This study helps the engineers understand the H_(2)S removal for sulfur-containing UGS as well as provides technical guidelines for converting other multilayered sour gas fields into underground storage sites.

A new transition metal diphosphideα-MoP_(2) synthesized by a high-temperature and high-pressure technique

Monoclinicα-MoP_(2),with the OsGe2-type structure(space group C2/m,Z=4)and lattice parameters a=8.7248(11)Å,b=3.2322(4)Å,c=7.4724(9)Å,andβ=119.263°,was synthesized under a pressure of 4~GPa at a temperature between 1100℃and 1200℃.The structure ofα-MoP_(2) and its relationship to other transition metal diphosphides are discussed.Surprisingly,the ambient pressure phase orthorhombicβ-MoP_(2)(space group Cmc21)is denser in structure thanα-MoP_(2).Room-temperature high-pressure x-ray diffraction studies exclude the possibility of phase transition fromβ-MoP_(2) to α-MoP_(2),suggesting thatα-MoP_(2) is a stable phase at ambient conditions;this is also supported by the total energy and phonon calculations.

Doping tuned anomalous Hall effect in the van der Waals magnetic topological phases Mn(Sb_(1-x)Bi_(x))_(4)Te_(7)

The van der Waals(vdW)MnSb4Te7is a newly synthesized antiferromagnetic(AFM)topological insulator hosting a robust axion insulator state irrelative to the specific spin structure.However,the intrinsic hole doped character of MnSb_4Te_7makes the Fermi level far away from the Dirac point of about 180 meV,which is unfavorable for the exploration of exotic topological properties such as the quantum anomalous Hall effect(QAHE).To shift up the Fermi level close to the Dirac point,the strategy of partially replacing Sb with Bi as Mn(Sb_(1-x)Bi_(x))_(4)Te_(7)was tried and the magnetotransport properties,in particular,the anomalous Hall effect,were measured and analyzed.Through the electron doping,the anomalous Hall conductanceσAH changes from negative to positive between x=0.3 and 0.5,indicative of a possible topological transition.Besides,a charge neutrality point(CNP)also appears between x=0.6 and 0.7.The results would be instructive for further understanding the interplay between nontrivial topological states and the magnetism,as well as for the exploration of exotic topological properties.

Effects of platelets on characteristics of lymphocytes cultured in vitro and optimization of adoptive immunotherapy

T lymphocytes,the main participants of cellular immunity,can express a variety of surface molecules and form different lymphocyte subsets under the induction of different factors to play the functions of immune regulation and immune killing.Studies have shown that platelets play a crucial role in maintaining the stable differentiation of lymphocytes and the balance in immunomodulation.Therefore,it is necessary to study the effect of platelets on lymphocytes in vitro to better understand the role of platelets in the immune system and broaden the application of adoptive immunotherapy.Methods:Cell counting and microscopic observation were used to detect the effect of activated platelets on lymphocyte proliferation in vitro;Flow cytometry was used to detect whether changes in platelet activity affect the proportion of lymphocyte subpopulations in vitro,and to detect differences in the expression of granzyme B;lactate dehydrogenase assay(LDH)was used to determine the difference in lymphocyte killing activity caused by platelet activity in vitro.Results:This was the first to promote lymphocyte proliferation through the expression or release of certain molecules in vitro,demonstrating that platelet activation is one of the key factors.Secondly,activated platelets or inactivated platelets promoted lymphocyte subset differentiation by enhancing the proportion of CD3+CD8+T lymphocytes(CTL cells)but had a slight effect on the proportion of CD3+CD4+T(Th cells)and CD4+CD25+T lymphocytes(Treg cells).Then,it was found that either activated platelets or inactivated platelets down-regulated the proportion of natural killer(NK)T lymphocytes,while activated platelets significantly enhance the proportion of NK lymphocytes.Therefore,by further detecting the killing activity of PBMCs treated with platelets,it was found that activated platelets promoted the extensive anti-tumor activity of lymphocytes and significantly increased the expression of granzyme B.Conclusion:Our results suggest that activated platelets promote lymphocyte proliferation,optimize lymphocyte subpopulation ratio,and promote cytotoxic effect of lymphocytes in vitro,which may provide a new strategy for optimizing the adoptive immunotherapy culture system and improving its efficacy.

A Survey of the Researches on Grid-Connected Solar Power Generation Systems and Power Forecasting Methods Based on Ground-Based Cloud Atlas

Photovoltaic power generating is one of the primary methods of utilizing solar energy resources,with large-scale photovoltaic grid-connected power generation being the most efficient way to fully utilize solar energy.In order to provide reference strategies for pertinent researchers as well as potential implementation,this paper tries to provide a survey investigation and technical analysis of machine learning-related approaches,statistical approaches and optimization techniques for solar power generation and forecasting.Deep learning-related methods,in particular,can theoretically handle arbitrary nonlinear transformations through proper model structural design,such as hidden layer topology optimization and objective function analysis to save information that can increase forecasting accuracy while filtering out irrelevant or less affected data for forecasting.The research’s results indicate that RBFNN-AG performed the best when applying the predetermined number of days,with an NRMSE value of 4.65%.RBFNN-AG performs better than sophisticated models like DenseNet(5.69%),SLFN-ELM(5.95%),and ANN-k-means-linear regression correction(6.11%).Additionally,scenario application and PV system investment techniques are provided to evaluate the current condition of new energy development and market trends both domestically and internationally.