Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We rece...Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.展开更多
Snow samples were collected over a 3-year period from 2012 to 2014 at the Hailuogou glacier of Mountain Gongga(Mt. Gongga) and analyzed for 16 priority polycyclic aromatic hydrocarbons(PAHs) using Gas Chromatography–...Snow samples were collected over a 3-year period from 2012 to 2014 at the Hailuogou glacier of Mountain Gongga(Mt. Gongga) and analyzed for 16 priority polycyclic aromatic hydrocarbons(PAHs) using Gas Chromatography–Mass Spectrometry(GC–MS). The results show that total average levels of the 16 PAHs ranged from 452 to 290 ng·L^(-1) with a possible declining trend from 2012 to 2014. Distances between the sampling sites and the emission sources were estimated at typically less than 500 km. The results suggest that the major source of PAHs was from coal combustion, while contributions from automobile exhaust played an important role in more recent years. This finding was in agreement with the characteristics of presence of local industry, residence, and recent development of tourism of the surrounding areas.展开更多
Endothelial-to-mesenchymal transition(EndMT)is a key driver of atherosclerosis.Aerobic glycolysis is increased in the endothelium of atheroprone areas,accompanied by elevated lactate levels.Histone lactylation,mediate...Endothelial-to-mesenchymal transition(EndMT)is a key driver of atherosclerosis.Aerobic glycolysis is increased in the endothelium of atheroprone areas,accompanied by elevated lactate levels.Histone lactylation,mediated by lactate,can regulate gene expression and participate in disease regulation.However,whether histone lactylation is involved in atherosclerosis remains unknown.Here,we report that lipid peroxidation could lead to EndMT-induced atherosclerosis by increasing lactatedependent histone H3 lysine 18 lactylation(H3K18la)in vitro and in vivo,as well as in atherosclerotic patients’arteries.Mechanistically,the histone chaperone ASF1A was first identified as a cofactor of P300,which precisely regulated the enrichment of H3K18la at the promoter of SNAI1,thereby activating SNAI1 transcription and promoting EndMT.We found that deletion of ASF1A inhibited EndMT and improved endothelial dysfunction.Functional analysis based on Apoe^(KO)Asf1a^(ECKO) mice in the atherosclerosis model confirmed the involvement of H3K18la in atherosclerosis and found that endotheliumspecific ASF1A deficiency inhibited EndMT and alleviated atherosclerosis development.Inhibition of glycolysis by pharmacologic inhibition and advanced PROTAC attenuated H3K18la,SNAI1 transcription,and EndMT-induced atherosclerosis.This study illustrates precise crosstalk between metabolism and epigenetics via H3K18la by the P300/ASF1A molecular complex during EndMT-induced atherogenesis,which provides emerging therapies for atherosclerosis.展开更多
以饲料玉米为研究对象,采用化学计量学方法,利用全波段光谱数据建立玉米粗蛋白预测的简单快速精准预测模型。结果表明:原始光谱经去趋势算法预处理后,Rank-KS算法选择校正集和预测集,使用偏最小二乘(Partial least square,PLS)方法进行...以饲料玉米为研究对象,采用化学计量学方法,利用全波段光谱数据建立玉米粗蛋白预测的简单快速精准预测模型。结果表明:原始光谱经去趋势算法预处理后,Rank-KS算法选择校正集和预测集,使用偏最小二乘(Partial least square,PLS)方法进行建模,校正集和预测集的相关系数分别为0.9915和0.9813,校正集和预测集的均方根误差分别为0.0634和0.1138。预测集的相对分析误差RPD为5.02,大于评估阈值3.0。所建模型精度和稳定性较为理想,可满足在线生成检测的要求。展开更多
The past several decades have witnessed great progress in the research on coordination polymers since the conception of supramolecular chemistry was advanced by Prof. Lehn, especially after the report of Prof. Robson
Electrolyte regeneration is an important goal for environmental protection and sustainable development efforts.Herein,we report a facile strategy inspired by the transformation of edible dough from flour to regenerate...Electrolyte regeneration is an important goal for environmental protection and sustainable development efforts.Herein,we report a facile strategy inspired by the transformation of edible dough from flour to regenerate hydrogel electrolytes from their dehydrated copolymer granules(CGs)via direct addition of water or salt solution.With the aid of heating,this procedure is efficient,relatively quick,and easily implemented.The dehydrated CGs are lightweight,reusable and stable under long-term storage.Even after 5 cycles of dehydration and regeneration,the regeneration efficiency of the hydrogel electrolytes,as evaluated based on retention of mechanical strength,is over 60%.The regenerated electrolytes possess considerable ionic conductivity,reprocessability,and 3D-printability.Furthermore,an all-gel supercapacitor assembled from the regenerated hydrogel electrolyte and activated carbon electrode with CGs as binder demonstrates excellent interfacial compatibility.The assembled all-gel supercapacitor can maintain 98.7% of its original specific capacitance after 100 bending tests,and can operate in a wide temperature range spanning from-15 to 60°C.This work may provide a new access to the development of renewable materials for various applications in the fields of intelligent devices,wearable electronics and soft robotics.展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000)。
文摘Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.
基金supported by the National Natural Science Foundation of China(41073085,41573014)the program of Sichuan Province for research innovation team of universities(12TD001)
文摘Snow samples were collected over a 3-year period from 2012 to 2014 at the Hailuogou glacier of Mountain Gongga(Mt. Gongga) and analyzed for 16 priority polycyclic aromatic hydrocarbons(PAHs) using Gas Chromatography–Mass Spectrometry(GC–MS). The results show that total average levels of the 16 PAHs ranged from 452 to 290 ng·L^(-1) with a possible declining trend from 2012 to 2014. Distances between the sampling sites and the emission sources were estimated at typically less than 500 km. The results suggest that the major source of PAHs was from coal combustion, while contributions from automobile exhaust played an important role in more recent years. This finding was in agreement with the characteristics of presence of local industry, residence, and recent development of tourism of the surrounding areas.
基金supported by the National Natural Science Foundation of China(82270421,81970428,31771334,81800385,82270484,82370376,82121001,82030013,and 82241211)Major Research Plan of the National Natural Science Foundation of China(91649125)+3 种基金the National Key R&D Program of China(2019YFA0802704)The major project of Natural Science Foundation of the Jiangsu Higher Education Institution of China(21KJA310006)Jiangsu Provincial Social Development Project(BE2021749,China)Special Program for Top Innovative Talents(NJMUTY20230082,China).
文摘Endothelial-to-mesenchymal transition(EndMT)is a key driver of atherosclerosis.Aerobic glycolysis is increased in the endothelium of atheroprone areas,accompanied by elevated lactate levels.Histone lactylation,mediated by lactate,can regulate gene expression and participate in disease regulation.However,whether histone lactylation is involved in atherosclerosis remains unknown.Here,we report that lipid peroxidation could lead to EndMT-induced atherosclerosis by increasing lactatedependent histone H3 lysine 18 lactylation(H3K18la)in vitro and in vivo,as well as in atherosclerotic patients’arteries.Mechanistically,the histone chaperone ASF1A was first identified as a cofactor of P300,which precisely regulated the enrichment of H3K18la at the promoter of SNAI1,thereby activating SNAI1 transcription and promoting EndMT.We found that deletion of ASF1A inhibited EndMT and improved endothelial dysfunction.Functional analysis based on Apoe^(KO)Asf1a^(ECKO) mice in the atherosclerosis model confirmed the involvement of H3K18la in atherosclerosis and found that endotheliumspecific ASF1A deficiency inhibited EndMT and alleviated atherosclerosis development.Inhibition of glycolysis by pharmacologic inhibition and advanced PROTAC attenuated H3K18la,SNAI1 transcription,and EndMT-induced atherosclerosis.This study illustrates precise crosstalk between metabolism and epigenetics via H3K18la by the P300/ASF1A molecular complex during EndMT-induced atherogenesis,which provides emerging therapies for atherosclerosis.
文摘以饲料玉米为研究对象,采用化学计量学方法,利用全波段光谱数据建立玉米粗蛋白预测的简单快速精准预测模型。结果表明:原始光谱经去趋势算法预处理后,Rank-KS算法选择校正集和预测集,使用偏最小二乘(Partial least square,PLS)方法进行建模,校正集和预测集的相关系数分别为0.9915和0.9813,校正集和预测集的均方根误差分别为0.0634和0.1138。预测集的相对分析误差RPD为5.02,大于评估阈值3.0。所建模型精度和稳定性较为理想,可满足在线生成检测的要求。
文摘The past several decades have witnessed great progress in the research on coordination polymers since the conception of supramolecular chemistry was advanced by Prof. Lehn, especially after the report of Prof. Robson
基金financially supported by the National Key R&D Program of China(2018YFC1803100 and 2016YFA0100800)the National Natural Science Foundation of China(51873156).
文摘Electrolyte regeneration is an important goal for environmental protection and sustainable development efforts.Herein,we report a facile strategy inspired by the transformation of edible dough from flour to regenerate hydrogel electrolytes from their dehydrated copolymer granules(CGs)via direct addition of water or salt solution.With the aid of heating,this procedure is efficient,relatively quick,and easily implemented.The dehydrated CGs are lightweight,reusable and stable under long-term storage.Even after 5 cycles of dehydration and regeneration,the regeneration efficiency of the hydrogel electrolytes,as evaluated based on retention of mechanical strength,is over 60%.The regenerated electrolytes possess considerable ionic conductivity,reprocessability,and 3D-printability.Furthermore,an all-gel supercapacitor assembled from the regenerated hydrogel electrolyte and activated carbon electrode with CGs as binder demonstrates excellent interfacial compatibility.The assembled all-gel supercapacitor can maintain 98.7% of its original specific capacitance after 100 bending tests,and can operate in a wide temperature range spanning from-15 to 60°C.This work may provide a new access to the development of renewable materials for various applications in the fields of intelligent devices,wearable electronics and soft robotics.
