Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis

AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time points.In a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs group.Serum AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into rats.In both studies,the protective effect of MSCs was evaluated.RESULTS:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time point.In vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP group.Serum SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 h.Intestinal mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting MSCs.CONCLUSION:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic inflammation in rats with SAP.

Polymer electrolyte with composite cathode for solid-state Li–CO_2 battery

The rechargeable Li-CO2 battery has attracted much attention for energy storage because of the high energy density and efficient utilization of greenhouse gas. However, it＇s still suffered by low safety issue of liquid electrolyte. Herein, a composite cathode consisting of CNTs and polymer electrolytes was fabricated by the insitu polymerization process for the polymer electrolyte-based solid-state Li-CO2 batteries. With the good dispersion of CNTs and polymer electrolyte, the composite cathode is covered by film-like discharge products Li2CO3.Furthermore, the Li-CO2 battery shows high reversible capacity （- 11,000 mAh·g^-1）, excellent cycle stability （1000 mAb·g^-1 for 100 cycles） under low charge potential （〈 4.5 V）, and outstanding rate performances at room temperature, which are much better than those of liquid electrolyte-based battery. Therefore, the polymer electrolyte-based Li-CO2 battery prepared by this strategy can be a promising candidate to meet the demands of high safety and high-performance energy storage devices.