During insect larval-pupal metamorphosis,proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis;however,the type of proteins and how these proteins are internalized int...During insect larval-pupal metamorphosis,proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis;however,the type of proteins and how these proteins are internalized into the fat body are unclear.In Bombyx mori,the developmental profiles o f total proteins in the hemolymph and fat body showed that hemolymph-decreased protein bands(55-100 kDa)were in accordance with those protein bands that increased in the fat body.Inhibition o f clathrin-dependent endocytosis predominantly blocked the transportation o f 55-100 kDa proteins from the hemolymph into the fat body,which was further verified by RNA interference treatment o f Bmclathrin.Six hexamerins were shown to comprise〜90%of the total identified proteins in both the hemolymph and fat body by mass spectrum(MS)analysis.In addition,hemolymphspecific proteins were mainly involved in material transportation,while fat body-specific proteins particularly participated in metabolism.In this paper,four hexamerins were found for the first time,and potential proteins absorbed by the fat body from the hemolymph through clathrin-dependent endocytosis were identified.This study sheds light on the protein absorption mechanism during insect metamorphosis.展开更多
基金This study was supported by the National Natu-ral Science Foundation of China(grants 31472042 and 31672368 to LT,31702053 to KL and 31702065 to HY)the Natural Science Foundation of Guangdong Province(grant 2017A030311024 to LT)。
文摘During insect larval-pupal metamorphosis,proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis;however,the type of proteins and how these proteins are internalized into the fat body are unclear.In Bombyx mori,the developmental profiles o f total proteins in the hemolymph and fat body showed that hemolymph-decreased protein bands(55-100 kDa)were in accordance with those protein bands that increased in the fat body.Inhibition o f clathrin-dependent endocytosis predominantly blocked the transportation o f 55-100 kDa proteins from the hemolymph into the fat body,which was further verified by RNA interference treatment o f Bmclathrin.Six hexamerins were shown to comprise〜90%of the total identified proteins in both the hemolymph and fat body by mass spectrum(MS)analysis.In addition,hemolymphspecific proteins were mainly involved in material transportation,while fat body-specific proteins particularly participated in metabolism.In this paper,four hexamerins were found for the first time,and potential proteins absorbed by the fat body from the hemolymph through clathrin-dependent endocytosis were identified.This study sheds light on the protein absorption mechanism during insect metamorphosis.
