Objective To filtrate breast cancer resistance protein （BCRP）-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance. Methods MTT assay was performed to filtra...Objective To filtrate breast cancer resistance protein （BCRP）-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance. Methods MTT assay was performed to filtrate BCRP-mediated resistant agents with BCRP expression cell model and to detect chemosensitivity of breast cancer tissue specimens to these agents. A high performance liquid chromatography （HPLC） assay was established, and was used to measure the relative dose of intracellular retention resistant agents. RT-PCR and immunohistochemistry （IHC） were employed to investigate the BCRP expression in breast cancer tissue specimens. Results MTT assay showed that the expression of BCRP increased with the increasing resistance of 5-fluorouracil （5-Fu） （P〈0.05, n=3） in the cell model, while HPLC assay indicated that the intracellular retention dose of 5-Fu was significantly correlated with the expression of BCRP （t=-0.897, P〈0.05, n=3）. A total of 140 breast cancer tissue specimens were collected. BCRP-positive expression was detected in forty-seven specimens by both RT-PCR and IHC. As shown by MTT assay subsequently, the resistance index （RI） of 47 BCRP-positive breast cancer tissue specimens to 5-Fu was 7-12 times as high as that of adjacent normal tissue samples. BCRP expression was related to 5-Fu resistance （R2=0.8124, P〈0.01）. Conclusion Resistance to 5-Fu can be mediated by BCRR Clinical chemotherapy for breast cancer patients can be optimized based on BCRP-positive expression.展开更多
Industrial application of superhydrophobic surfaces is often hindered by complicated process and sophisticated machines. A facile wet etching method （sandblast, HCl and sandblast/HCl） with vapor deposition of PFDS ...Industrial application of superhydrophobic surfaces is often hindered by complicated process and sophisticated machines. A facile wet etching method （sandblast, HCl and sandblast/HCl） with vapor deposition of PFDS （1H, 1H, 2H, 2H- perfluorodecyltriethoxysilane） was applied to fabricate superhydrophobic surface of heat-resistant steel used for vane. The coating component, surface morphology and surface roughness parameters of sample were observed by attenuated total reflectance Fourier transform infrared spectroscopy, scanning electron microscopy and atomic force microscopy. Static water contact angle （WCA） of samples with and without PFDS coating was measured by contact angle goniometer. The results showed that WCA values of polished, sandblast, HCl and sandblast/HCl-etched samples are 98°, 97°, 100° and 101°, respectively, and increase to 112°, 148°, 151 ° and 154v after vapor deposition of PFDS. The sandblast/HCl-etched sample with PFDS coating shows higher superhydrophobicity because of very large surface roughness and lotus protrusionlike structure. The superhydrophobicity of this fabricated surface has no obvious change after 38 cycles of the film adhesion test, indicating excellent durability.展开更多
基金the National Basic Research Program of China (No. 2002CB512903)the National Natural Science Foundation of China (No. 30500599)
文摘Objective To filtrate breast cancer resistance protein （BCRP）-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance. Methods MTT assay was performed to filtrate BCRP-mediated resistant agents with BCRP expression cell model and to detect chemosensitivity of breast cancer tissue specimens to these agents. A high performance liquid chromatography （HPLC） assay was established, and was used to measure the relative dose of intracellular retention resistant agents. RT-PCR and immunohistochemistry （IHC） were employed to investigate the BCRP expression in breast cancer tissue specimens. Results MTT assay showed that the expression of BCRP increased with the increasing resistance of 5-fluorouracil （5-Fu） （P〈0.05, n=3） in the cell model, while HPLC assay indicated that the intracellular retention dose of 5-Fu was significantly correlated with the expression of BCRP （t=-0.897, P〈0.05, n=3）. A total of 140 breast cancer tissue specimens were collected. BCRP-positive expression was detected in forty-seven specimens by both RT-PCR and IHC. As shown by MTT assay subsequently, the resistance index （RI） of 47 BCRP-positive breast cancer tissue specimens to 5-Fu was 7-12 times as high as that of adjacent normal tissue samples. BCRP expression was related to 5-Fu resistance （R2=0.8124, P〈0.01）. Conclusion Resistance to 5-Fu can be mediated by BCRR Clinical chemotherapy for breast cancer patients can be optimized based on BCRP-positive expression.
文摘Industrial application of superhydrophobic surfaces is often hindered by complicated process and sophisticated machines. A facile wet etching method （sandblast, HCl and sandblast/HCl） with vapor deposition of PFDS （1H, 1H, 2H, 2H- perfluorodecyltriethoxysilane） was applied to fabricate superhydrophobic surface of heat-resistant steel used for vane. The coating component, surface morphology and surface roughness parameters of sample were observed by attenuated total reflectance Fourier transform infrared spectroscopy, scanning electron microscopy and atomic force microscopy. Static water contact angle （WCA） of samples with and without PFDS coating was measured by contact angle goniometer. The results showed that WCA values of polished, sandblast, HCl and sandblast/HCl-etched samples are 98°, 97°, 100° and 101°, respectively, and increase to 112°, 148°, 151 ° and 154v after vapor deposition of PFDS. The sandblast/HCl-etched sample with PFDS coating shows higher superhydrophobicity because of very large surface roughness and lotus protrusionlike structure. The superhydrophobicity of this fabricated surface has no obvious change after 38 cycles of the film adhesion test, indicating excellent durability.
