Latent profile analysis of perceived stress and influencing factors in colorectal cancer patients

Objective:To understand the latent categories of perceived stress in colorectal cancer patients and analyze the characteristics of different categories of patients.Methods:A total of 255 colorectal cancer patients receiving treatment in the gastrointestinal surgery and oncology depar tments of a ter tiary Grade A hospital in Sichuan Province,from January 2023 to June 2023,were selected as the study subjects.General information questionnaire,Chinese version of the Perceived Stress Scale(CPSS),and Comprehensive Score Table for Patient-Repor ted Outcome Measures of Economic Toxicity(COST-PROM)were used for data collection.Results:Perceived stress in colorectal cancer patients was classified into 3 latent categories:C1“Low stress-stable type”(19.2%),C2“Moderate stress-uncontrolled type”(23.9%),and C3“High stress-anxious type”(56.9%).The average score of perceived stress was(34.07±5.08).Compared with C1 type,patients with a monthly household income of≤3000 RMB were more likely to belong to the C2 and C3 types(P<0.05),and patients without a stoma were less likely to belong to C3 type(P<0.05).Compared with C2 type,male patients were more likely to belong to C3 type(P<0.05),and patients without a stoma were less likely to belong to C3 type(P<0.05).Compared with C3 type,patients with higher economic toxicity scores were more likely to be classified into C1 and C2 types(P<0.05).Conclusions:Perceived stress in colorectal cancer patients exhibits distinct categorical features.Male gender,lower income,presence of a stoma,and higher economic toxicity are associated with higher levels of perceived stress in colorectal cancer patients.

In-situ investigation of deformation behavior and fracture forms of Ti/Al/Mg/Al/Ti laminates

In-situ bending and stretching were conducted on hot-rolled and annealed Ti/Al/Mg/Al/Ti laminates,with a focus on crack initiation and propagation of intermetallics and component layers,which helps to clarify their deformation behavior and fracture forms.The results show that delamination is the early fracture form of laminate with or without intermetallics at Al/Mg interface,so Al/Mg interfacial bonding strength determines the mechanical properties of laminate.Various and irregular intermetallics cracks lead to Al/Mg interface delamination in annealed laminate and help to release stress.Necking and fracture of component layers are observed at the late deformation stage,and the sequence is Al,Mg and Ti layers,resulting from their strength.Angle between crack propagation direction and stretching direction of Mg layer both in rolled and annealed laminates is around 45°due to the effect of shear deformation,and crack convergence leads to final complete fracture of Mg layer.

Identification and activity evaluation of Astragalus Radix from different germplasm resources based on specific oligosaccharide fragments

Objective: Based on trifluoroacetic acid(TFA) hydrolysis, polyacrylamide gel electrophoresis(PAGE) and high performance thin layer chromatography(HPTLC) analysis, the carbohydrate responsible for immunomodulatory activity are used as quality indicators for Astragalus Radix(AR).Methods: In this study, 24 batches of AR from different germplasm resources were selected as the research object, and AR polysaccharides were extracted. PAGE and HPTLC methods were used to analyze the partial acid hydrolyzate of AR polysaccharides and obtain a series of saccharide fingerprints. The data were analyzed by principal component analysis to obtain the difference between AR from different germplasm resources.Results: The results showed that trisaccharide and tetrasaccharide could be used as differential fragments to distinguish AR of different cultivation methods;Disaccharides and trisaccharides can be used as differential fragments to distinguish different species of AR. The immunological activity analysis of the specific oligosaccharide fragment of AR showed that the specific oligosaccharide fragment of AR could promote the secretion of TNF-α, IL-1β, IL-6, and NO in THP-1 cells in a concentration-dependent manner.Conclusion: Both PAGE and HPTLC methods can be used to evaluate AR from different germplasm resources. This study laid the foundation for the quality evaluation of AR medicinal herbs.